期刊：
2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010),2010年:1-4 ISSN：2151-7614
通讯作者：
Luo, Qin
作者机构：
[Hu, Jing; Wang, Xingyun; Luo, Qin; Chu, Peili; Zhou, Qingchun] Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Luo, Qin] H;Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)
会议时间：
2010-06-18
会议地点：
成都
会议主办单位：
[Chu, Peili;Wang, Xingyun;Hu, Jing;Luo, Qin;Zhou, Qingchun] Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.
会议论文集名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)论文集
关键词：
plinton;Nematostella vectensis
摘要：
With genome-wide screening of N. vectensis, two new groups of Polinton were identified, beside five Polinton groups deposited in Repbase. The typical N. vectensis Polinton is in length of 15-20kb and has long TIRs with a 6-bp TSDs. it encodes as many as 6 different proteins, including retroviral-like integrase (INT), adenovirus protease (PRO), DNA polymerase B (POLB) and putative ATPase (ATP). These ancient N. vectensis Polinton groups fall to two clades by phylogenetic analysis. One clade evolves evolves to vertebrates together with large scale gene linkage of N. vectensis, another clade following to the evolution of fly and nematode genome.
摘要：
There are 15 strains of phosphorus-accumulating organisms were isolated from the soil of Phosphate Fertilizer Factory in HongHu. Among them, HS-P2 is one of the efficient phosphate accumulating organisms. Metachromatic granule staining displayed that poly-P granules exist in cells, and HS-P2 can be identified to Enterobacterspp according to morphological identification and 16SrDNA sequence alignment results. Under the aerobic condition, the curve of phosphorus dynamic showed that HS-P2 can remove the phosphorus to the maximum of 85% in the PO43- - P of 50mg / L medium at 48h, this study also showed that the optimal carbon source of HS-P2 is glucose, and the optimal pH is 7.0. It has a wide range of temperature for phosphorus removal of 30 degrees C to 37 degrees C, which indicated that HS-P2 has a strong ability in phosphorus-accumulating,and a broad prospects in practical application.
摘要：
The ability of Listeria monocytogenes resisting bile stress is very important for its successful infection and colonization in the human gastrointestinal tract. Some studies have indicated that the alternative sigma factor Sigma B (σB), encoded by sigB, is at least partly involved in bacteria resisting many adverse conditions. In order to investigate the role of L. monocytogenes Sigma B in bile tolerance in the gastrointestinal tract, sigB mutant strain EGDΔsigB was constructed, and in parallel with wild type strain EGD, exposed to 3% bile salt（sublethal concentration for bacteria）with or without pre-exposure to acid (pH 4.5), alkali (pH 9.0), or osmotic (0.3M NaCl) conditions by mimicking the human gastrointestinal juice for 30 min. Their growth and survival rates were examined after 5 hours cultivation. The results were shown (ⅰ) wild type strain EGD was more resistant to 3% bile salt stress than sigB mutant strain EGDΔsigB; (ⅱ) Both alkali- and osmotic-adapted EGD and EGDΔsigB were more resistant to 3% bile salt than nonadapted strains; (iii) acid-adapted EGD and EGDΔsigB didn't increase their ability to resist 3% bile salt. These data indicate that L. monocytogenes σB is required for bile tolerance in the gastrointestinal tract, and also reveal some σB-dependent cross-protection mechanisms involving in tolerance of alkaline (or osmolality) and bile salt stress.
作者机构：
[Qin, Long-juan; Yang, Xu; Wang, Meng; Wang, Jing; Cai, Feng-yun] Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
会议名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)
会议时间：
2010-06-18
会议地点：
成都
会议主办单位：
[Cai, Feng-yun;Wang, Meng;Wang, Jing;Qin, Long-juan;Yang, Xu] Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
会议论文集名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)论文集
关键词：
DBP;SOD;MDA;DPC;oxidative damage
摘要：
Di-n-butyl phthalate (DBP) was used in the plastic industry and widely distributed in the environment, which might cause adverse effects to living organisms. Previously, it had been shown that DBP produced reproductive, developmental toxicity and liver enlargement in rodents, and the mechanisms behind this could involve oxidative injury. To study the effects of DBP on the acute oxidative damage, the liver cells of Wistar rats were exposed to different concentration of DBP (0, 5, 20 and 80 mu mol.L-1) for one hour in vitro, then Nitro blue tetrazolium (NBT)-illumination method, Thiobarbituric acid (TBA) assay and KCl-SDS assay were used to measure the change of the activities of surperoxide dismutase (SOD), the contents of malondialdehyde (MDA) and the coefficient of DNA-protein crosslinks (DPC) respectively. With the increase of DBP concentrations, statistical significant decrease of the SOD activities, increase of MDA contents and DPC coefficient were observed. These results suggested that DBP could induce the acute oxidative damage in liver cells of rat.
摘要：
Melamine is an organic base and a trimer of cyanamide, with a 1,3,5-triazine skeleton or simply, it's an azocyclo-containing organic compound, and traditionally used in the chemical and sporadically mixed into animal feeds to boost protein content. This study was designed to test the melamine toxicity effects on aquatic ecosystem. S.obliquus was exposed to series melamine concentrations (0, 50, 200, 750 mg.L-1). The growth rate, super oxidase dismutase (SOD), photosynthetic pigments, free malondialdehyde (MDA) contents of S.obliquus were measured after 7d exposure. Melamine concentration of 50, 200mg.L-1 caused a significant increase in growth and photosynthetic pigments contents. The SOD activities of S. obliquus were stimulated first and then inhibited with the increasing of melamine concentrations, and reached the peak in 200mg.L-1 (compared with the control, p < 0.05). The contents of MDA were inhibited significantly under moderate and high concentration (50, 200, 750 mg.L-1) of melamine. This study indicates that, SOD was sensitive to melamine exposure and can be used as a potential biomarker for risk assessment of aquatic ecosystem safety.
摘要：
In this study, artificial antigens for Bensulfuron-methyl (BSM) were prepared by means of two-step procedure of glutaraldehyde method. The hapten BSM was covalently attached to ovalbumin to synthesis immunogen (BSM-OVA), and hapten Benzyl sulfonamide (BS) was linked to BSA to prepare coating antigen (BS-BSA). The conjugates were identified by UV spectrophotometry and SDS-PAGE. After immunized with BSM-OVA to BALB/c mice, high titer and sensitive polyclonal antibodies against bensulfuron-methyl herbicide were obtained. The titer was 1:1.28 × 10 5 . The IC 50 was 0.032 μg/mL. The results indicated that the artificial antigen for BSM synthesized successfully, and it could be used to produce monoclonal antibodies for BSM.
会议论文集名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)论文集
关键词：
Formaldehyde;Marrow cells;Cell cycle
摘要：
In order to explore the possibility of inhalation of formaldehyde on mouse bone marrow cells have an impact on cell cycle and use SPF level purely Kunming male mice for the study, with a concentration of 0.5 mg /m(3), 1.0 mg/m(3) and 3.0 mg/m(3) of gaseous Formaldehyde dynamics in mice exposed to continuous 72 hours after the exposure using flow cytometry analysis cells from mouse bone marrow cells, cell cycle changes. The results showed that: exposure group and control group the cell cycle there is an obvious difference between the data. These results suggest that: In the concentration range of formaldehyde on mouse bone marrow cells of the cell cycle changes in the role of clear.
摘要：
Methyl parathion hydrolase (MPD) is the key enzyme for methyl parathion degradation. MPD has been widely used to wipe off organophosphorus pesticides. Usually, recombinant MPD was expressed in E.coli BL21 cells predominantly as inclusion bodies with little active enzyme to collect. In this paper, we studied the denaturing of MPD inclusion bodies by urea as well as the protein renatured by diluting and dialyzing. We investigated the effect of urea concentration on the denature efficiency of MPD inclusion bodies;and the certain factors affecting the, efficiency of protein renaturation. These factors are including renature buffer components, pH, GSH/GSSG, and the protein concentration in the renature complex. The results suggested that the optimum urea concentration is 8 M for denature, and the optimum renature condition was 49 g/ml denature proteins diluted in Tris-HCl buffer (pH 8.5) plus 5 mM GSH, 0.5 mM GSSG and 1.5 M urea. Consequently, the renatured MPD reached the electrophoresis purity and its activity was 2.4 U/ml. The specific activity of the MPD reached at 48.8 U/mg, and the renature ratio of the recombinant MPD was 12.5% comparing with the activity of that expressed as soluble proteins.
摘要：
Owing to its good flame-retardant effect, polybrominated diphenyl ethers (PBDEs) were widely added to electronic, electrical appliances, as well as textiles and chemical products. Decabromodiphenyl Ether (BDE-209) is one class of PBDEs which was most demanded in international market. To illustrate the possible function of BDE-209 on rat liver, hepatic cells were expose to BDE-209 with different concentrations (1, 2, 4, 8, 16 mg center dot L-1) in vitro, the contents of malondialdehyde (MDA) and the levels of DNA-protein crosslinks (DPC) were investigated. The results indicated that: with the BDE-209 exposure concentrations increased, MDA content and DPC coefficient increased gradually, showed almost the same trend. Significant difference was observed in both MDA content and DPC coefficient in the 4,8 and 16 mg center dot L-1 (P < 0.05) groups compared to the control group, while extremely significant difference was observed in the 16 mg center dot L-1 groups (P < 0.01). Moreover, MDA content has a good dose-response relationship with the BDE-209 exposure concentrations(R= 0.995, P < 0.05), so does DPC (R= 0.996, P < 0.05). The results suggested that the BDE209 exposure can cause oxidative stress and genetic toxicity to rat liver, demonstrating hepatotoxic effect as well as genetic effect to the body.
摘要：
Nitrite reductase (nirS) is a key enzyme of denitrification catalyzing the one electron reduction of nitrite (NO2-) to nitrogen monoxide (NO). In this study, nirS gene was cloned from Pseudomonas aeruginosa HGP9 strain. The phylogenetic tree was constructed and the secondary structure was predicted by bioinformatics. Results showed that nirS gene was 99.8% similar to the nitrite reductase from Pseudomonas aeruginosa NCTC 6750 strain. Most of alpha-helices stretches are formed in the first 1/4 of the sequence and the beta-sheets are present in the last 3/4 sequences. Homology modeling based on using nitrite reductase (PDBID: 1gjqA) as template for nirS indicated that two independent subunits comprised a homodimer in nirS crystal structure, and each monomer is composed of a c-heme domain and a d1-heme domain. both of them are cytochrom super family. Compared to the three-dimensional structure of nirS, there was a nature mutation in nirS that located the site residue of Phe109 in P. aeruginosa HGP9 strain (the residue was Thr84 in P. aeruginosa NCTC 6750 strain), and the mutation site was in the heme c domain.
摘要：
15 bacterial strains with a capacity of poly-phosphate were isolated from enrichment culture of the soil sample collected from the phosphate fertilizer factory of Honghu City in Hubei province, of which one strain named as HS-P7 has the strongest effects of poly-phosphate. Ammonium molybdate spectrophotometric method was used to measure its ability of poly-phosphate. The results showed that phosphorus removal rate about the strain HS-P7 reached to 90.08% when it was cultivated in the phosphorus-rich medium in 30. during 72 hours. The homology between the strain HS-P7 and Pseudomonas cf. Monteilii is 98% by comparing their 16S rDNA. Based on the characteristics of morphological, physiological and biochemical, the strain HS-P7 was identified as Pseudomonas sp. The effects of different temperatures, pH, carbon sources and nitrogen sources on the growth of the strain HS-P7 were investigated and the optimum growth condition was determined as pH 7.0 and 30 degrees C.
摘要：
Phenol is an important and common environmental pollutant. In this study, Pseudomonas aeruginosa HS-D38 strain was used to degrade phenol, and the degradation characteristics were investigated. Results indicated that HS-D38 strain could degrade phenol and utilize it as the sole carbon, nitrogen and energy source. UV-visible spectra analysis showed that 300 mgL-1 phenols could be completely degraded within 10 h by HS-D38 strain after an initial lag phase of 2 h, and the peak decreased gradually with the degradation of phenol by HS-D38. At the same time, an increase of OD600 values in the culture was also observed. Phenol could be mineralized at higher concentration of 500 mgL-1 by HS-D38 strain, whereas the degradation did not occur when the concentration was exceeded 700 mgL-1 even after 72 h. A key intermediate was detected analyzing the degradation solution with HPLC and it was identified to p-hydroxybenzoic acid. The peaks disappeared after 15 h indicating intermediates were also degraded by HS-D38 strain and the metabolism proceeded through the p-hydroxybenzoic acid pathway.
摘要：
This study evaluated the effect of butyl benzyl phthalate(BBP), an important class of environmental endocrine disruptors on SOD, AChE, CAT of Danio rerio. Based on the BBP LC50, zebrafish were exposed to different BBP concentrations (0, 0.332, 0.665, 1.33 mg.L-1). The activities were determined in the fish collected at 7d, 14d, 21d and 28d post-exposure. It showed that as the extension of time and the exposure concentrations, from 0d to 28d, the AChE activity was significantly inhibited. The SOD activity increased firstly and then declined with time extension. The CAT activity, in.7d, except 0.665 mg . L-1 group was inhibited significantly (p < 0.01), the rest groups were of no significant difference compared with the control. At 14d, except 0.332 mg . L-1 group, which was always higher than the control, it showed a downward trend after the first rise for other groups; for 28d, the CAT activity of each treated group showed induction effect compared with the control.
摘要：
In order to explore distant-site genotoxic effect of gaseous formaldehyde on genetic material of marrow cells in mice. SPF-class Kun Ming male mice were exposed to gaseous formaldehyde for 72 hours in such concentration as 0.5mg/m(3), 1.0mg/m(3) and 3.0mg/m(3). We applied the random amplified polymorphic DNA (RAPD) method to evaluate the genotoxic effect of the gaseous formaldehyde. The results of the RAPD profiles showed obvious differences between normal and exposed groups. The number of the varied bands increased gradually with the concentration of gaseous formaldehyde. Genomic template stability (GTS) analysis showed that with the increase of the gaseous formaldehyde concentration, the polymorphisms were50%, 39.66%, 32.76% and the GTS were 50%, 60.34%, 67.24% respectively. These results suggest that gaseous formaldehyde has significant genotoxicity and damage effect on the genetic material in marrow cells in this concentration range. Meanwhile, the DNA polymorphisms detected by RAPD technique could be used as a biomarker for detecting genetic effects of formaldehyde stress on mice.
作者机构：
[Yang Xu; Wang Li; Wei Chen-xi] Huazhong Normal Univ, Coll Life Sci, Lab Environm Sci, Wuhan, Peoples R China.;[Tang Li-ya] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Hong Kong, Hong Kong, Peoples R China.
会议名称：
4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE)
会议时间：
JUN 18-20, 2010
会议地点：
Chengdu, PEOPLES R CHINA
会议主办单位：
[Wang Li;Wei Chen-xi;Yang Xu] Huazhong Normal Univ, Coll Life Sci, Lab Environm Sci, Wuhan, Peoples R China.^[Tang Li-ya] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Hong Kong, Hong Kong, Peoples R China.
会议论文集名称：
International Conference on Bioinformatics and Biomedical Engineering
摘要：
The effect of Di-(2-ethylhexyl) Phthalate was investigated when alga Scenedesmus obliquus has been exposed to different concentrations(5, 10, 20, 40 and 80 mg center dot L-1) at 24, 48, 72and 96 hours of exposure. Toxicity test was done by using four biomarkers including growth inhibition rate and the content of chlorophyll a, chlorophyll b and carotenoid. This study indicated that with the increase concentration of Di-(2-ethylhexyl) Phthalate, the alga inhibition rate went up in a dose dependent manner. The effect of Di-(2-ethylhexyl) Phthalate on the changes of pigment content showed the treatment groups were significantly decrease compared with control group(P < 0.01). The content of carotenoid was hardly changed while it significantly decreased in higher concentration (80 mg.L-1) (p < 0.01). It indicated that DEHP has acute toxicity on S. obliquus.
期刊：
2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010),2010年:1-3 ISSN：2151-7614
通讯作者：
Xu, Yang
作者机构：
[Xu, Yang; Yang, Wu; Qing, Chang] Huazhong Normal Univ, Coll Life Sci, Lab Environm Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Xu, Yang] H;Huazhong Normal Univ, Coll Life Sci, Lab Environm Sci, Wuhan 430079, Peoples R China.
会议名称：
International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2010)
会议时间：
2010-01-01
会议地点：
Chengdu, China
会议主办单位：
[Yang, Wu;Qing, Chang;Xu, Yang] Huazhong Normal Univ, Coll Life Sci, Lab Environm Sci, Wuhan 430079, Peoples R China.
会议论文集名称：
2010 4th International Conference on Bioinformatics and Biomedical Engineering. [v.1.1]
关键词：
8-OHdG;oxidative DNA damage;rat liver cell;formaldehyde
摘要：
we developed a quantitative detection method that can reflect the degree of visceral cell oxidative DNA damage induced by exogenous compound exposures using 8-OHdG as a biomarker in vitro. In this study formaldehyde (FA) was used as a model exogenous compound and malondialdehyde (MDA) was used as a reference indicator. Rat liver cell suspension was applied in this in vitro exposure experiments as testing material. The liver cells of rat were exposed to different concentrations of FA (0, 5, 15, 45 mu mol center dot L-1) for 1h, then the contents of 8-OHdG and MDA were measured. Research results showed that the 8-OHdG and MDA were increased gradually with the increase of formaldehyde concentration. At high FA concentration level (45 mu mol center dot L-1) the 8-OHdG and MDA contents are extremely different (P < 0.01) when compared with the control group, at the middle FA level (15 mu mol center dot L-1) their differences are significant (P < 0.01, P < 0.05), and at the low level (5 mu mol center dot L-1) there is no significant difference (P > 0.05). The experimental results also showed that the dose-effect relationship between FA and 8OHdG seems much better than that between FA and MDA. The conclusion is that 8-OHdG not only can be used for blood and urine sample testing, but also a good biomarker of visceral cell DNA oxidative damage, as it was explored in this study.
摘要：
We measured the airborne dust retention ability of leaves taken from 28 specie of plants planted along the main roads at centre urban area in Wuhan. We also ranked plants by their dust retention ability at first tree layer, second tree layer and shrub layer, respectively. The results showed that Platanus acerifolia, Firmiana simplex among 5 big tree species and Loropetalum chinense var., rubrum among 14 shrub species have the strongest dust retention ability. All the species at the second tree layer have weak dust retention ability. The principle and measure to dispose plants along the main roads should according to the plant ecology traits, ornamental value and the dust airborne quantity.
期刊：
2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010),2010年:1-4 ISSN：2151-7614
通讯作者：
Liao Xiaomei
作者机构：
[Liao Xiaomei; Xie Min; Wang Shaohui; Cheng Liang; Chen Qicai] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.
通讯机构：
[Liao Xiaomei] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.
会议名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)
会议时间：
2010-06-18
会议地点：
成都
会议主办单位：
[Cheng Liang;Xie Min;Wang Shaohui;Chen Qicai;Liao Xiaomei] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.
会议论文集名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)论文集
关键词：
white noise;oxidative stress;hippocampus;tau phosphorylation
摘要：
White noise makes peroxidative damage of the brain, dose it have effect on the phosphorylation of tau in hippocampus? We used 66 mice exposed to 80 dB SPL white noise for 3 days, 1 week, and 3 weeks respectively, then detected the changes of Superoxide dismulase (SOD) activity and malondialdehyde (MDA) content in inferior colliculus (IC), auditory cortex (AC), and hippocampus. Meanwhile we detected the levels of phosphorylation of microtubule-associated protein tau in hippocampus after 1 week and 3 weeks noise exposure. We found that compared with the control group, the value of SOD/MDA of the three brain regions especially the hippocampus of the noise group decreased significantly. At the same time the phosphporylation of tau increased obviously. Our results indicated that the moderate white noise exposure can induced peroxidative damage in IC, AC, and hippocampus; and the peroxidative damage may involved in tau hyperphosphorylation of hippocampus through the lemniscal path.
摘要：
In the past decade, rapid development happened in the field of nanotechnology. Recently, nano-material, especially inorganic hybrid molecule, has become an increasingly hot issue in medicine area for safe drug delivery system. In this paper, nano-layered double hydroxides (nano-LDHs) were chosen to be a potential drug carrier. In order to explore the biocompatibility of nano-LDHs with He la cells, a single cell gel electrophoresis and H2DCF-DA fluorescent coloration assay were used to analyze the DNA damage and the change of reactive oxygen species (ROS) in He la cells exposed to nano-LDHs. Results showed that, with the increase of concentrations of nano-LDHs (0 similar to 800 mu g.mL(-1)), the Tail DNA%, Tail Moment and ROS contents in He la cells increased gradually; there were significant differences (p < 0.05, p < 0.01) in those parameters between high concentration groups (200, 400, 800 mu g.mL-1) and the control group, while no significant differences (p > 0.05) were found between low concentration groups (50, 100 mu g.mL(-1)) and the control. The results suggested that the low concentration nano-LDHs (<= 100 mu g.mL(-1)) had a good biocompatibility with He la cells, indicating that nano-LDHs may be applicable in medicine.
