Ocnus is essential for male germ cell development in Drosophila melanogaster
作者：
Zheng, Y.;Bi, J.;Hou, M. -Y.;Shen, W.;Zhang, W.;...
期刊：
Insect Molecular Biology ,2018年27(5):545-555 ISSN：0962-1075
通讯作者：
Wang, Y. -F.
作者机构：
[Wang, Y. -F.; Bi, J.; Ai, H.; Zheng, Y.; Shen, W.; Yu, X. -Q.; Hou, M. -Y.; Zhang, W.] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.;[Yu, X. -Q.] Univ Missouri, Sch Biol Sci, Kansas City, MO 64110 USA.;[Wang, Y. -F.] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Wang, Y. -F.] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
关键词：
ocnus;Drosophila melanogaster;RNA-seq;testis;germ cell
摘要：
The ocnus (ocn) gene encodes a protein abundant in the testes, implying its role in testis development. When Drosophila melanogaster is infected with the endosymbiont wMel Wolbachia, which affects the spermatogenesis of its hosts, ocn is downregulated in the third-instar larval testes, suggesting a role of ocn in spermatogenesis. In this study, we knocked down ocn in the testes and found that the hatch rates of embryos derived from ocn-knockdown males were significantly decreased, and 84.38% of the testes were much smaller in comparison to controls. Analysis of the smaller testes showed no germ cells but they had an extended hub. Using RNA-sequencing (RNA-Seq), we identified 69 genes with at least a twofold change (q-value < 5%) in their expression after ocn knockdown; of these, eight testes-specific and three reproduction-related genes were verified to be significantly downregulated using quantitative reverse transcription-PCR. Three genes (orientation disruptor, p24-2 and CG13541) were also significantly downregulated in the presence of Wolbachia. Furthermore, 98 genes were not expressed when ocn was knocked down in testes. These results suggest that ocn plays a crucial role in male germ cell development in Drosophila, possibly by regulating the expression of multiple spermatogenesis-related genes. Our data provide important information to help understand the molecular regulatory mechanisms underlying spermatogenesis. © 2018 The Royal Entomological Society
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Exposure to a combination of formaldehyde and DINP aggravated asthma-like pathology through oxidative stress and NF-κB activation
作者：
Kang, Jun;Duan, Jiufei;Song, Jing;Luo, Chen;Liu, Hong;...
期刊：
Toxicology ,2018年404-405:49-58 ISSN：0300-483X
通讯作者：
Chen, Mingqing;Yu, Wei
作者机构：
[Chen, Mingqing; Duan, Jiufei; Yang, Xu; Kang, Jun; Song, Jing; Luo, Chen] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Yu, Wei; Li, Baizhan; Liu, Hong] Chongqing Univ, Minist Educ, Joint Int Lab Green Bldg & Built Environm, Chongqing 400045, Peoples R China.
通讯机构：
[Chen, Mingqing; Yu, Wei] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;Chongqing Univ, Minist Educ, Joint Int Lab Green Bldg & Built Environm, Chongqing 400045, Peoples R China.
关键词：
*Asthma;*Diisononyl phthalate (DINP);*Formaldehyde;*NF-kappaB;*Oxidative stress
摘要：
Several epidemiological and experimental studies indicate a positive association between exposure to formaldehyde or phthalates and allergic asthma. However, nothing is yet known about the effects of exposure to for-maldehyde and phthalates together, nor the role of each on allergic asthma. Here, we investigated the effects of a combined exposure to formaldehyde and diisononyl phthalate (DINP) on asthma-like pathology in mice, and determined the underlying mechanisms implicated in NF-kappa B and ROS. Mice were exposed to formaldehyde and/or DINP and sensitization with OVA. The results showed that exposure to 1.0 mg/m(3) formaldehyde or 20 mg/kg.d DINP slightly aggravated the airway wall remodeling, promoted the production of IgE and IgG1, and induced the occurrence of airway hyperresponsiveness (AHR). However, these pathological responses and AHR were greatly exacerbated by the combined exposure to formaldehyde and DINP. Administering melatonin to block oxidative stress, alleviated the pathological responses and AHR induced by formaldehyde and DINP, and inhibited the activation of the NF-kappa B and the secretion of TSLP. Blocking NF-kappa B with Dehydroxymethylepoxyquinimicin, inhibited the elevation of TSLP expression and Th2/Th17 cytokine secretion, and effectively alleviated the allergic asthma-like symptoms. The results suggested that exposure to both formaldehyde and DINP aggravated hypersensitivity asthma symptoms by promoting oxidative stress and activating NF-kappa B. These findings expand our understanding of how formaldehyde and DINP exposure affect the development of allergic asthma.
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Resistance to Bacillus thuringiensis linked with a cadherin transmembrane mutation affecting cellular trafficking in pink bollworm from China
作者：
Wang, Ling;Ma, Yuemin;Wan, Peng;Liu, Kaiyu;Xiao, Yutao;...
期刊：
Insect Biochemistry and Molecular Biology ,2018年94:28-35 ISSN：0965-1748
通讯作者：
Wu, Kongming
作者机构：
[Wu, Kongming; Wang, Ling] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.;[Wang, Jintao; Cong, Shengbo; Wan, Peng; Xu, Dong; Wang, Ling] Hubei Acad Agr Sci, Inst Plant Protect & Soil Fertil,Minist Agr, Key Lab Integrated Pest Management Crops Cent Chi, Hubei Key Lab Crop Dis Insect Pests & Weeds Contr, Wuhan 430064, Hubei, Peoples R China.;[Liu, Kaiyu; Ma, Yuemin] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Xiao, Yutao] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China.;[Wang, Jintao] Huazhong Agr Univ, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Hubei, Peoples R China.
通讯机构：
[Wu, Kongming] C;Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.
关键词：
*Bacillus thuringiensis;*Cry1Ac;*Genetically engineered crop;*Pectinophora gossypiella;*Transgenic cotton
摘要：
Evolution of pest resistance reduces the efficacy of insecticidal proteins from the gram-positive bacterium Bacillus thuringiensis (Bt) used widely in sprays and transgenic crops. In some previously studied strains of three major lepidopteran pests, resistance to Bt toxin Cry1Ac is associated with mutations disrupting the extracellular or cytoplasmic domains of cadherin proteins that bind Cry1Ac in the midgut of susceptible larvae. Here we report the first case of a cadherin transmembrane mutation associated with insect resistance to Bt. We discovered this mutation in a strain of the devastating global cotton pest, the pink bollworm (Pectinophora gossypiella), derived from a field population in the Yangtze River Valley of China. The mutant allele analyzed here has a 207 base pair deletion and encodes a cadherin protein lacking its transmembrane domain. Relative to a susceptible strain, a strain homozygous for this allele had 220-fold resistance to Cry1Ac and 2.1-fold cross-resistance to Cry2Ab. On transgenic cotton plants producing Cry1Ac, no susceptible larvae survived, but the resistant strain completed its life cycle. Inheritance of resistance to Cry1Ac was autosomal, recessive and tightly linked with the cadherin gene. Transportation of cadherin protein to the cell membrane and susceptibility to Cry1Ac occurred in transfected insect cells expressing the wild type cadherin allele, but not in transfected insect cells expressing the mutant cadherin allele. The results imply that the mutant allele analyzed here confers resistance to Cry1Ac by disrupting cellular trafficking of cadherin. © 2018 Elsevier Ltd
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Exposure to DBP and High Iodine Aggravates Autoimmune Thyroid Disease Through Increasing the Levels of IL-17 and Thyroid-Binding Globulin in Wistar Rats
作者：
Duan, Jiufei;Kang, Jun;Deng, Ting;Yang, Xu;Chen, Mingqing*
期刊：
Toxicological Sciences ,2018年163(1):196-205 ISSN：1096-6080
通讯作者：
Chen, Mingqing
作者机构：
[Chen, Mingqing; Duan, Jiufei; Deng, Ting; Yang, Xu; Kang, Jun] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Chen, Mingqing] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
High iodine;di-n-butyl phthalate;thyroxine-binding globulin;IL-17;AP-1
摘要：
Autoimmune thyroid disease (AITD) is the most common autoimmune disease that causes hypothyroidism. High iodine is a well-known factor that can induce thyroid disorders, including Hashimoto’s thyroiditis, one of the main types of AITD. Recent epidemiological studies have indicated that phthalates, especially di-n-butyl phthalate (DBP) may induce thyroid disease. In this study, we aim to determine the effects and underlying mechanisms of high iodine and/or DBP exposure on AITD. Female Wistar rats were modeled with thyroglobulin and exposed to high iodine and/or DBP. We investigated histopathological changes in the thyroid and measured thyroid hormone levels in serum to assess thyroid function. In the thyroid and liver, we detected oxidative stress, proinflammatory factors (IL-1β, IL-6, and IL-17) and the activation of activator protein 1 (AP-1), a transcription factor that is related to the synthesis of the thyroxine-binding globulin (TBG) and the activation of Th17. After blocking AP-1 with SP600125, we detected TBG and the Th17 related cytokines (IL-6 and IL-17). The data showed that thyroid damage and the alteration of thyroid hormones were greater when the rats were exposed to both high iodine and DBP. Coexposure to DBP and high iodine enhanced the activation of AP-1 in the liver and thyroid, and induced an increase in the levels of TBG in serum and IL-17 in the thyroid. Blocking AP-1 activation prevented the increase of TBG and IL-17. The results indicate that high iodine and/or DBP exposure exacerbated AITD through altering TBG levels in serum and aggravating IL-17 in the thyroid.
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英文
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Heterostyly promotes compatible pollination in buckwheats: Comparisons of intraflower, intraplant, and interplant pollen flow in distylous and homostylous Fagopyrum
作者：
Wu, Ling-Yun;Chang, Fang-Fang;Liu, Shu-Juan;Armbruster, W. Scott;Huang, Shuang-Quan*
期刊：
American Journal of Botany ,2018年105(1):108-116 ISSN：0002-9122
通讯作者：
Huang, Shuang-Quan
作者机构：
[Huang, Shuang-Quan; Chang, Fang-Fang; Wu, Ling-Yun] Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Hubei, Peoples R China.;[Liu, Shu-Juan] Jiangxi Acad Sci, Inst Biol Resources, Nanchang 330012, Jiangxi, Peoples R China.;[Armbruster, W. Scott] Univ Portsmouth, Sch Biol Sci, King Henry Bldg, Portsmouth PO1 2DY, Hants, England.;[Armbruster, W. Scott] Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK 99709 USA.
通讯机构：
[Huang, Shuang-Quan] C;Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Hubei, Peoples R China.
关键词：
disassortative pollination;distyly;homostyly;pollen fate;pollen transfer proficiency;Polygonaceae;self pollination;stigmatic pollen loads
摘要：
Premise of the Study: Heterostyly, the reciprocal positioning of stigmas and anthers in different floral morphs, has long been thought to promote intermorph pollination. However, extensive intramorph pollination occurs commonly in heterostylous species, leading to recurrent questions about the functional and evolutionary significance of heterostyly. Methods: To identify the sources of stigmatic pollen (autogamous [intraflower], geitonogamous [intraplant], vs. interplant), we emasculated either one flower or entire plants in experimental populations of the two closely related buckwheat species, distylous Fagopyrum esculentum and homostylous F. tataricum. Differences in pollen size allowed unambiguous identification of pollen on stigmas. Results: Only 2.4% of F. tataricum pollen and 1.5% of F. esculentum pollen arrived successfully on compatible stigmas of other plants. In the former (homostylous) species, 71.3% of the pollen load on stigmas was autogamous, 10.8% was geitonogamous, and 17.9% was interplant. In the latter (distylous) species, 37.45% of the pollen on stigmas was autogamous, 13.8% was geitonogamous, 17.0% was intramorph, and 31.75% was intermorph. The amount of incompatible pollen arriving on stigmas was greatly decreased by both one-flower and whole-plant emasculations, and thus, the proportion of compatible pollen deposited increased with one-flower emasculation and increased even more with whole-plant emasculation. Conclusions: Our quantification of pollen-donor sources in these two species indicated that heterostyly in Fagopyrum esculentum provided a nearly 2-fold fitness advantage (in terms of compatible pollination) over expected (random) pollen transfers between morphs. Because of reduced herkogamy, the homostylous F. tataricum was highly autogamous. © 2018 Botanical Society of America
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英文
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Sinorhizobium meliloti, a Slow-Growing Bacterium, Exhibits Growth Rate Dependence of Cell Size under Nutrient Limitation
作者：
Dai, Xiongfeng;Shen, Zichu;Wang, Yiheng;Zhu, Manlu*
期刊：
mSphere ,2018年3(6) ISSN：2379-5042
通讯作者：
Zhu, Manlu
作者机构：
[Shen, Zichu; Dai, Xiongfeng; Zhu, Manlu; Wang, Yiheng] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Zhu, Manlu] C;Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.
关键词：
*Sinorhizobium meliloti;*cell cycle;*cell size;*growth rate
摘要：
Bacterial cells need to coordinate the cell cycle with biomass growth to maintain cell size homeostasis. For fast-growing bacterial species like Escherichia coli and Bacillus subtilis , it is well-known that cell size exhibits a strong dependence on the growth rate under different nutrient conditions (known as the nutrient growth law). However, cell size changes little with slow growth (doubling time of >90 min) for E. coli , posing the interesting question of whether slow-growing bacteria species also observe the nutrient growth law. Here, we quantitatively characterize the cell size and cell cycle parameter of a slow-growing bacterium, Sinorhizobium meliloti , at different nutrient conditions. We find that S. meliloti exhibits a threefold change in its cell size when its doubling time varies from 2 h to 6 h. Moreover, the progression rate of its cell cycle is much longer than that of E. coli , suggesting a delicate coordination between the cell cycle progression rate and the biomass growth rate. Our study shows that the nutrient growth law holds robustly regardless of the growth capacity of the bacterial species, generalizing its applicability among the bacterial kingdom. IMPORTANCE The dependence of cell size on growth rate is a fundamental principle in the field of bacterial cell size regulation. Previous studies of cell size regulation mainly focus on fast-growing bacterial species such as Escherichia coli and Bacillussubtilis . We find here that Sinorhizobium meliloti , a slow-growing bacterium, exhibits a remarkable growth rate-dependent cell size pattern under nutrient limitation, generalizing the applicability of the empirical nutrient growth law of cell size. Moreover, S. meliloti exhibits a much slower speed of cell cycle progression than E. coli does, suggesting a delicate coordination between the cell cycle progression rate and the biomass growth rate.
语种：
英文
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Characterization of bHLH/HLH genes that are involved in brassinosteroid (BR) signaling in fiber development of cotton (Gossypium hirsutum)
作者：
Lu, Rui;Zhang, Jiao;Liu, Dong;Wei, Ying-Li;Wang, Yao;...
期刊：
BMC Plant Biology ,2018年18(1):1-13 ISSN：1471-2229
通讯作者：
Li, Xue-Bao
作者机构：
[Wang, Yao; Zhang, Jiao; Wei, Ying-Li; Lu, Rui; Li, Xue-Bao; Liu, Dong] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Li, Xue-Bao] C;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
关键词：
Cotton (Gossypium hirsutum);bHLH/HLH transcription factor;Fiber development;Phylogenetic analysis;Brassinosteroid (BR) signaling;Gene expression
摘要：
Background: Basic helix-loop-helix/helix-loop-helix (bHLH/HLH) transcription factors play important roles in plant development. Many reports have suggested that bHLH/HLH proteins participate in brassinosteroid (BR) hormone signaling pathways to promote cell elongation. Cotton fibers are single-cells and derived from seed surface. To explore the roles of bHLH/HLH proteins in cotton fiber development progress by modulating BR signaling pathway, we performed a systematic analysis of the bHLH/HLH gene family in upland cotton (Gossypium hirsutum) genome. Results: In this study, we identified 437 bHLH/HLH genes in upland cotton (G. hirsutum) genome. Phylogenetic analysis revealed that GhbHLH/HLH proteins were split into twenty six clades in the tree. These GhbHLH/HLH genes are distributed unevenly in different chromosomes of cotton genome. Segmental duplication is the predominant gene duplication event and the major contributor for amplification of GhbHLH/HLH gene family. The GhbHLH/HLHs within the same group have conserved exon/intron pattern and their encoding proteins show conserved motif composition. Based on transcriptome data, we identified 77 GhbHLH/HLH candidates that are expressed at relatively high levels in cotton fibers. As adding exogenous BR (brassinolide, BL) or brassinazole (Brz, a BR biosynthesis inhibitor), expressions of these GhbHLH/HLH genes were up-regulated or down-regulated in cotton fibers. Furthermore, overexpression of GhbHLH282 (one of the BR-response genes) in Arabidopsis not only promoted the plant growth, but also changed plant response to BR signaling. Conclusion: Collectively, these data suggested that these GhbHLH/HLH genes may participate in BR signaling transduction during cotton fiber development. Thus, our results may provide a valuable reference data as the basis for further studying the roles of these bHLH/HLH genes in cotton fiber development. © 2018 The Author(s).
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英文
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Acute formaldehyde exposure induced early Alzheimer-like changes in mouse brain
作者：
Liu, Xudong;Zhang, Yuchao;Wu, Ruliu;Ye, Meng;Zhao, Yuqing;...
期刊：
Toxicology Mechanisms and Methods ,2018年28(2):95-104 ISSN：1537-6516
通讯作者：
Yang, Xu
作者机构：
[Wu, Ruliu; Zhao, Yuqing; Li, Jinquan; Yang, Xu; Kang, Jun; Ye, Meng; Liu, Xudong] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Lab Environm Biomed, Wuhan, Hubei, Peoples R China.;[Zhang, Yuchao; Liu, Xudong] Moutai Coll, Dept Food Sci & Engn, Renhuai, Peoples R China.;[Ma, Ping] Hubei Univ Sci & Technol, Sch Basic Med Sci, Res Ctr Basic Med Sci, Xianning, Peoples R China.;[Li, Jinquan] NYU, Sch Med, Tuxedo Pk, NY USA.;[Yang, Xu] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Yang, Xu] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
Alzheimer's disease;early Alzheimer-like changes;formaldehyde;inflammation;oxidative stress
摘要：
Alzheimer’s disease (AD) is a serious, common, global disease, yet its etiology and pathogenesis are incompletely understood. Although an association between AD and exposure to air pollutants has been discussed, the effects of pollutants on the functioning of the brain remain unclear. The indoor environment is where exposure to formaldehyde (FA) can occur. Whether exposure to FA contributes to the development of AD needs to be investigated. To determine the objective, C57BL/6 mice were exposed daily to FA (0, 0.155, 1.55 and 15.5 mg/kg/day) for 1 week. After acute FA exposure, some early AD-like changes [cognitive deficits, pathological alterations in the mouse brain, accumulation of total β-amyloid plaques 1-42 (Aβ1-42) and hyper-phosphorylated tau (Tau-P) in the cerebral cortex] were detected after exposure to high concentrations of FA (1.55 or 15.5 mg/kg/day). The permeability of the blood–brain barrier (BBB), activation of astrocyte and microglia, oxidative stress (OS) and inflammation were analyzed to explore the toxicity mechanisms behind the development of early AD-like changes. While exposed to a low concentration of FA (0.155 mg/kg/day) had little or no adverse effects on the mouse brain. The results indicated that acute FA exposure induced early AD-like changes in mouse brain, increased the susceptibility of AD in mouse. © 2017 Informa UK Limited, trading as Taylor & Francis Group.
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英文
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Computational and functional characterization of four SNPs in the SOST locus associated with osteoporosis
作者：
Ye, Weiyuan;Wang, Ya;Mei, Bing;Hou, Sasa;Liu, Xinhong;...
期刊：
Bone ,2018年108:132-144 ISSN：8756-3282
通讯作者：
Huang, Qingyang
作者机构：
[Wu, Guiju; Hou, Sasa; Liu, Xinhong; Huang, Qingyang; Mei, Bing; Ye, Weiyuan; Wang, Ya; Zhao, Kehui; Qin, Longjuan] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Huang, Qingyang] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
*MicroRNA;*Osteoporosis;*SNPs;*SOST;*Transcription factors
摘要：
The SOST gene encodes sclerostin, a C-terminal cysteine knot-like domain containing key negative regulator of osteoblastic bone formation that inhibits LRP5/6-mediated canonical Wnt signaling. Numerous single nucleotide polymorphisms (SNPs) in the SOST locus are firmly associated with bone mineral density (BMD) and fracture in genome-wide association studies (GWAS) and candidate gene association studies. However, the validation and mechanistic elucidation of causal genetic variants, especially for SNPs located beyond the promoter-proximal region, remain largely unresolved. By employing computational and experimental approaches, here we identify four SNPs rs1230399, rs7220711, rs1107748 and rs75901553 as functional variants which display allelic variation in SOST gene expression. The osteoporosis associated SNP rs1230399 in the SOST distal upstream regulatory region shows FOXA1 binding activity with subsequent transinactivation in a T allele-specific manner. The BMD GWAS lead SNPs rs7220711 and rs1107748 both reside in the 52-kb regulatory element deletion 35-kb downstream of the SOST gene which leads to Van Buchem disease. The rs7220711-A has a higher affinity for the transcriptional repressors MAFF or MAFK homodimers than rs7220711-G, while rs1107748 confers C allele specific transcriptional enhancer activity via a CTCF binding element. The variant rs75901553 C > T located in a conserved site of the SOST 3′ UTR abolishes a target binding site for miR-98-5p which is negatively responsive to parathyroid hormone or 17β-estradiol in osteoblastic cell lines. Our findings uncover the biological consequences of four independent genetic variants in the SOST region and their important roles in SOST expression via diverse mechanisms, providing new insights into the genetics and molecular pathogenesis of osteoporosis. © 2018
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英文
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Formaldehyde regulates vascular tensions through nitric oxide-cGMP signaling pathway and ion channels
作者：
Zhang, Quanxi* ;Tian, Peiru;Zhai, Miaomiao;Lei, Xiaodong;Yang, Zhenhua;...
期刊：
Chemosphere ,2018年193(Feb.):60-73 ISSN：0045-6535
通讯作者：
Zhang, Quanxi;Yang, Xu
作者机构：
[Liu, Yan; Zhai, Miaomiao; Liu, Mengting; Zhang, Xiri; Huang, Hao; Lei, Xiaodong; Yang, Zhenhua; Tian, Peiru; Zhang, Quanxi; Meng, Ziqiang] Shanxi Univ, Inst Environm Sci, Inst Environm Med & Toxicol, Taiyuan 030006, Shanxi, Peoples R China.;[Yang, Xu; Zhao, Yun] Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Lab Environm Biomed, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Zhang, Quanxi] S;[Yang, Xu] H;Shanxi Univ, Inst Environm Sci, Inst Environm Med & Toxicol, Taiyuan 030006, Shanxi, Peoples R China.;Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Lab Environm Biomed, Wuhan 430079, Hubei, Peoples R China.
关键词：
Formaldehyde;Thoracic aorta ring;Vascular tension;Ion channels;Signal pathway
摘要：
Formaldehyde (FA) has been linked to the detrimental cardiovascular effects. Here, we explored the effects and mechanisms of FA on rat aortas both in vivo and in vitro. The results presented that FA evidently lowered the blood pressures of rats. The expression levels of BK<inf>Ca</inf>subunits αand β1 and iNOS of the aortas were up-regulated by FA in vivo. However, FA markedly reduced the levels of Ca<inf>v</inf>1.2 and Ca<inf>v</inf>1.3, which are the subunits of L-Ca<sup>2+</sup>channel. Furthermore, the contents of NO, cGMP and iNOS in the aortas were augmented by FA. To further confirm these findings, the mechanisms accredited to these effects were examined in vitro. The data showed that FA contracted the isolated aortic rings at low concentrations (<300 μM), while it relaxed the rings at high concentrations (>500 μM). The FA-induced vasoconstriction at low concentrations was blocked partly by an inhibitor of ACE. The relaxation caused by FA at high concentrations was attenuated by the inhibitors of NO-cGMP pathway, L-Ca<sup>2+</sup>channel and BK<inf>Ca</inf>channel, respectively. Similarly, the expression of iNOS was strongly enhanced by FA in vitro. The effects of FA on the aortic rings with endothelium were significantly greater than those on the rings without endothelium. Our results indicate that the vasoconstriction of FA at low concentrations might be partially pertinent to endothelin, and the FA-caused vasorelaxation at high concentrations is possibly associated with the NO-cGMP pathway, L-Ca<sup>2+</sup>channel and BK<inf>Ca</inf>channel. This study will improve our understanding of the pathogenic mechanisms for FA-related cardiovascular diseases.<br/> ©2017 Elsevier Ltd
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英文
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Histone deacetylase 1 promotes glioblastoma cell proliferation and invasion via activation of PI3K/AKT and MEK/ERK signaling pathways
作者：
Li, Shun;Chen, Xiangrong;Mao, Lifang;Zahid, Kashif Rafiq;Wen, Jun;...
期刊：
Brain Research ,2018年1692:154-162 ISSN：0006-8993
通讯作者：
Wang, Xiangyu;Xu, Guozheng
作者机构：
[Wang, Xiangyu; Xu, Guozheng; Li, Shun] Southern Med Univ, Grad Sch, Guangzhou 510515, Guangdong, Peoples R China.;[Mao, Lifang; Zhao, Long; Duan, Jie; Yin, Xiaohong; Tang, Xiaoping; Li, Shun; Wang, Yuanchuan; Duan, Junwei] North Sichuan Med Coll, Dept Neurosurg, Affiliated Hosp, Nanchong 637000, Sichuan, Peoples R China.;[Wang, Xiangyu; Wen, Jun; Zhang, Liu; Zhang, Maoying; Li, Shun] Jinan Univ, Affiliated Hosp 1, Dept Neurosurg, 613 West Huangpu Rd, Guangzhou 510630, Guangdong, Peoples R China.;[Xu, Guozheng; Li, Shun] Wuhan Gen Hosp PLA, Dept Neurosurg, 627 Wu Luo Rd, Wuhan 430070, Hubei, Peoples R China.;[Chen, Xiangrong] Fujian Med Univ, Affiliated Hosp 2, Dept Neurosurg, Quanzhou 362000, Fujian, Peoples R China.
通讯机构：
[Wang, Xiangyu] J;[Xu, Guozheng] W;Jinan Univ, Affiliated Hosp 1, Dept Neurosurg, 613 West Huangpu Rd, Guangzhou 510630, Guangdong, Peoples R China.;Wuhan Gen Hosp PLA, Dept Neurosurg, 627 Wu Luo Rd, Wuhan 430070, Hubei, Peoples R China.
关键词：
HDAC1;Glioblastoma;Proliferation;Invasion;Signaling pathway
摘要：
Histone deacetylase 1 (HDAC1) plays a crucial role in cancer progression and development. This enzyme has been confirmed to be a key regulator of tumor biology functions, such as tumor cell proliferation, migration and invasion. However, HDAC1 expression in glioma remains controversial, and its specific function and molecular mechanism in glioblastoma is poorly understood. In this study, our findings demonstrated that protein and mRNA levels of HDAC1 were increased in glioma cell lines and glioma tissues compared to normal glial cell lines and non-neoplastic brain tissues, respectively. Furthermore, HDAC1 knockdown cells displayed decreased proliferation and invasion capabilities, whereas HDAC1 overexpressing glioblastoma cells displayed more proliferation and invasion capabilities in vitro. These novel outcomes suggested that knockdown of HDAC1 possibly suppressed the expression of phosphory-fated AKT (p-AKT) and phosphorylated ERIC (p-ERIC) proteins, while overexpression of HDAC1 significantly increased p-AKT and p-ERIC protein in glioblastoma cells. In addition, knockdown of HDAC1 repressed subcutaneous tumor growth in vivo, and led to down-regulation of p-AKT and p-ERIC protein in U87 MG xenograft tumors. For the first time, we have demonstrated that HDAC1 promotes proliferation and invasion in glioblastoma cells by activating P13K/AKT and MEK/ERK signaling pathways in vitro and in vivo. These results suggest that HDAC1 may be a novel biomarker and potential therapeutic target in glioblastoma. (C) 2018 Elsevier B.V. All rights reserved.
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英文
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On the intrinsic constraint of bacterial growth rate: M. tuberculosis’s view of the protein translation capacity
作者：
Zhu, Manlu* ;Dai, Xiongfeng*
期刊：
Critical Reviews in Microbiology ,2018年44(4):455-464 ISSN：1040-841X
通讯作者：
Zhu, Manlu;Dai, Xiongfeng
作者机构：
[Dai, Xiongfeng; Zhu, Manlu; Dai, XF] Cent China Normal Univ, Coll Life Sci, 152 Luoyu Ave, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Zhu, ML; Dai, XF] C;Cent China Normal Univ, Coll Life Sci, 152 Luoyu Ave, Wuhan 430079, Hubei, Peoples R China.
关键词：
Bacterial growth rate;Mycobacterium tuberculosis;active ribosome fraction;fraction of RNA polymerase engaged in rRNA synthesis;polypeptide chain elongation rate;rRNA chain elongation rate
摘要：
In nature, the maximal growth rates vary widely among different bacteria species. Fast-growing bacteria species such as Escherichia coli can have a shortest generation time of 20 min. Slow-growing bacteria species are perhaps best known for Mycobacterium tuberculosis, a human pathogen with a generation time being no less than 16 h. Despite of the significant progress made on understanding the pathogenesis of M. tuberculosis, we know little on the origin of its intriguingly slow growth. From a global view, the intrinsic constraint of the maximal growth rate of bacteria remains to be a fundamental question in microbiology. In this review, we analyze and discuss this issue from the angle of protein translation capacity, which is the major demand for cell growth. Based on quantitative analysis, we propose four parameters: rRNA chain elongation rate, abundance of RNA polymerase engaged in rRNA synthesis, polypeptide chain elongation rate, and active ribosome fraction, which potentially limit the maximal growth rate of bacteria. We further discuss the relation of these parameters with the growth rate for M. tuberculosis as well as other bacterial species. We highlight future comprehensive investigation of these parameters for different bacteria species to understand how bacteria set their own specific growth rates. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
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英文
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Acetylome Profiling Reveals Extensive Lysine Acetylation of the Fatty Acid Metabolism Pathway in the Diatom Phaeodactylum tricornutum
作者：
Chen, Zhuo;Luo, Ling;Chen, Runfa;Hu, Hanhua;Pan, Yufang;...
期刊：
Molecular & Cellular Proteomics ,2018年17(3):399-412 ISSN：1535-9476
通讯作者：
Gong, Yangmin
作者机构：
[Chen, Zhuo; Luo, Ling; Gong, Yangmin; Wan, Xia; Chen, Runfa] Chinese Acad Agr Sci, Minist Agr, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China.;[Chen, Zhuo] Shandong Normal Univ, Coll Life Sci, Key Lab Plant Stress Res, Jinan 250014, Shandong, Peoples R China.;[Hu, Hanhua; Jin, Hu; Pan, Yufang] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China.;[Jiang, Haibo] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Jiang, Haibo] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Gong, Yangmin] C;Chinese Acad Agr Sci, Minist Agr, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China.
摘要：
N-lysine acetylation represents a highly dynamic and reversibly regulated post-translational modification widespread in almost all organisms, and plays important roles for regulation of protein function in diverse metabolic pathways. However, little is known about the role of lysine acetylation in photosynthetic eukaryotic microalgae. We integrated proteomic approaches to comprehensively characterize the lysine acetylome in the model diatom Phaeodactylum tricornutum. In total, 2324 acetylation sites from 1220 acetylated proteins were identified, representing the largest data set of the lysine acetylome in plants to date. Almost all enzymes involved in fatty acid synthesis were found to be lysine acetylated. Six putative lysine acetylation sites were identified in a plastid-localized long-chain acyl-CoA synthetase. Site-directed mutagenesis and site-specific incorporation of N-acetyllysine in acyl-CoA synthetase show that acetylation at K407 and K425 increases its enzyme activity. Moreover, the nonenzymatically catalyzed overall hyperacetylation of acyl-CoA synthetase by acetyl-phosphate can be effectively deacetylated and reversed by a sirtuin-type NAD-dependent deacetylase with subcellular localization of both the plastid and nucleus in Phaeodactylum. This work indicates the regulation of acyl-CoA synthetase activity by site-specific lysine acetylation and highlights the potential regulation of fatty acid metabolism by lysine actetylation in the plastid of the diatom Phaeodactylum. © 2018 by The American Society for Biochemistry and Molecular Biology, Inc.
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英文
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A single amino acid polymorphism in ABCC2 loop 1 is responsible for differential toxicity of Bacillus thuringiensis Cry1Ac toxin in different Spodoptera (Noctuidae) species
作者：
Liu, Leilei;Chen, Zuwen;Yang, Yanchao;Xiao, Yutao;Liu, Chenxi;...
期刊：
Insect Biochemistry and Molecular Biology ,2018年100:59-65 ISSN：0965-1748
通讯作者：
Yang, Yongbo;Liu, Kaiyu;Bravo, Alejandra
作者机构：
[Yang, Yanchao; Liu, Kaiyu; Yang, Yongbo; Chen, Zuwen; Liu, Leilei; Ma, Yuemin] Cent China Normal Univ, Sch Life Sci, Inst Entomol, Wuhan 430079, Hubei, Peoples R China.;[Xiao, Yutao] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China.;[Liu, Chenxi] Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, West Yuanmingyuan Rd, Beijing 100193, Peoples R China.;[Soberon, Mario; Bravo, Alejandra] Univ Nacl Autonoma Mexico, Inst Biotecnol, Apdo Postal 510-3, Cuernavaca 62250, Morelos, Mexico.
通讯机构：
[Yang, YB; Liu, KY] C;[Bravo, Alejandra] U;Cent China Normal Univ, Sch Life Sci, Inst Entomol, Wuhan 430079, Hubei, Peoples R China.;Univ Nacl Autonoma Mexico, Inst Biotecnol, Apdo Postal 510-3, Cuernavaca 62250, Morelos, Mexico.
关键词：
*ABCC2 transporter;*Bacillus thuringiensis;*Cry toxin-resistance;*Helicoverpa armigera;*Spodoptera frugiperda;*Spodoptera litura
摘要：
Bacillus thuringiensis Cry toxins exert their toxicity by forming membrane pores after binding with larval midgut membrane proteins known as receptors. Spodoptera litura and Spodoptera frugiperda belong to the same genus, but S. litura is tolerant to Cry1Ac, while S. frugiperda is susceptible. The mechanism involved in the differential toxicity of Cry1Ac to these insect species is not understood. Amino acid sequences analysis of ABCC2, a well-recognized Cry1Ac receptor, from both species showed high sequence identity. Hi5 insect cells expressing SfABCC2 from S. frugiperda were 65-fold more susceptible than those expressing the SlABCC2 from S. litura. Substitution of fragments, point mutations and deletions between the ABCC2 of the two species revealed that ABCC2 amino acid Q 125 from SfABCC2 or E 125 from SlABCC2 was key factor for the differential Cry1Ac toxicity to Hi5 cells expressing these receptors. Consistently with this, cells expressing Helicoverpa armigera HaABCC2 Q122 -GFP, were more susceptible to Cry1Ac than cells expressing HaABCC2 E122 -GFP mutant. Q 125 or E 125 is located in a predicted exposed loop 1 region of ABCC2 indicating that this region could be important for Cry1Ac binding. These findings identified a single amino acid residue located in loop 1 of ABCC2 transporter as responsible for the different levels of susceptibility to Cry1Ac among various lepidopteran species. © 2018 Elsevier Ltd
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英文
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Generation, safety and immunogenicity of an Actinobacillus pleuropneumoniae quintuple deletion mutant SLW07 (ΔapxICΔapxIICΔorf1ΔcpxARΔarcA)
作者：
Yuan, Fangyan;Liu, Jinlin;You, Wujin;Bei, Weicheng;Wang, Chunlai;...
期刊：
Vaccine ,2018年36(14):1830-1836 ISSN：0264-410X
通讯作者：
Liu, Siguo;Tian, Yiongxiang
作者机构：
[Yuan, Fangyan; Tian, Yiongxiang] Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Key Lab Prevent & Control Agents Anim Bacteriosis, Minist Agr,Hubei Key Lab Anim Embryo & Mol Breedi, Wuhan 430064, Hubei, Peoples R China.;[Wang, Chunlai; Liu, Siguo] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Heilongjiang, Peoples R China.;[You, Wujin; Bei, Weicheng] Huazhong Agr Univ, Coll Vet Med, Cooperat Innovat Ctr Sustainable Pig Prod, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China.;[Zhao, Jin; Liu, Jinlin] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Liu, Siguo] C;[Tian, Yiongxiang] H;Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Heilongjiang, Peoples R China.;Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Key Lab Prevent & Control Agents Anim Bacteriosis, Minist Agr,Hubei Key Lab Anim Embryo & Mol Breedi, Wuhan 430064, Hubei, Peoples R China.
关键词：
*Actinobacillus pleuropneumoniae;*Protective efficacy;*Quintuple deletion mutant;*Safety
摘要：
We inactivated a virulence determinant, ArcA, in an Actinobacillus pleuropneumoniae quadruple deletion mutant SLW06 (Delta apxIC Delta apxIIC Delta orf1 Delta cpxAR, serovar 1), and a quintuple deletion mutant SLW07 was generated. SLW07 showed decreased adherence to and invasion of host cells, compared to its parent strain SLW06. SLW07 was more sensitive in RAW264.7 macrophage-mediated phagocytosis and clearance. SLW07 was less virulent in mice. An immunization assay indicated that both SLW07 and SLW06 preferentially stimulated T helper cell type 2 response in mice. Live vaccines induced the production of interleukin-6 and tumor necrosis factor-alpha by splenic lymphocytes. Furthermore, the protective immunity of SLW07 was not affected after ArcA mutation. Immunization with SLW07 could provide a complete protection following virulent A. pleuropneumoniae challenge in mice. Our results suggest that SLW07 is a promising live vaccine candidate, which is further attenuated from and shares similar protective efficacy with its quadruple deletion parent SLW06. (C) 2018 Elsevier Ltd. All rights reserved.
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英文
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The identification of IsiA proteins binding chlorophyll d in the cyanobacterium Acaryochloris marina
作者：
Li, Zheng-Ke;Yin, Yan-Chao;Zhang, Lu-Dan;Zhang, Zhong-Chun;Dai, Guo-Zheng;...
期刊：
Photosynthesis Research ,2018年135(1-3):165-175 ISSN：0166-8595
通讯作者：
Qiu, Bao-Sheng;Chen, Min
作者机构：
[Qiu, Bao-Sheng; Zhang, Lu-Dan; Li, Zheng-Ke; Dai, Guo-Zheng; Yin, Yan-Chao; Zhang, Zhong-Chun] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Qiu, Bao-Sheng; Zhang, Lu-Dan; Li, Zheng-Ke; Dai, Guo-Zheng; Yin, Yan-Chao; Zhang, Zhong-Chun] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Chen, Min] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.
通讯机构：
[Qiu, Bao-Sheng] C;[Chen, Min] U;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.
会议名称：
Light Harvesting Satellite Meeting held in conjunction with the 17th International Congress on Photosynthesis Research
会议时间：
AUG, 2016
会议地点：
Egmond aan Zee, NETHERLANDS
会议主办单位：
[Li, Zheng-Ke;Yin, Yan-Chao;Zhang, Lu-Dan;Zhang, Zhong-Chun;Dai, Guo-Zheng;Qiu, Bao-Sheng] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.^[Li, Zheng-Ke;Yin, Yan-Chao;Zhang, Lu-Dan;Zhang, Zhong-Chun;Dai, Guo-Zheng;Qiu, Bao-Sheng] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.^[Chen, Min] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.
关键词：
Accessory chlorophyll-binding proteins (CBPs);Iron limitation;Iron-stress-induced protein A (IsiA);Photosynthesis;Six transmembrane helix family of chlorophyll-binding proteins/antenna;Acaryochloris marina
摘要：
The bioavailable iron in many aquatic ecosystems is extremely low, and limits the growth and photosynthetic activity of phytoplankton. In response to iron limitation, a group of chlorophyll-binding proteins known as iron stress-induced proteins are induced and serve as accessory light-harvesting components for photosystems under iron limitation. In the present study, we investigated physiological features of Acaryochloris marina in response to iron-deficient conditions. The growth doubling time under iron-deficient conditions was prolonged to ~3.4 days compared with 1.9 days under normal culture conditions, accompanied with dramatically decreased chlorophyll content. The isolation of chlorophyll-binding protein complexes using sucrose density gradient centrifugation shows six main green bands and three main fluorescence components of 712, 728, and 748 nm from the iron-deficient culture. The fluorescence components of 712 and 728 nm co-exist in the samples collected from iron-deficient and iron-replete cultures and are attributed to Chl d-binding accessory chlorophyll-binding antenna proteins and also from photosystem II. A new chlorophyll-binding protein complex with its main fluorescence peak at 748 nm was observed and enriched in the heaviest fraction from the samples collected from the iron-deficient culture only. Combining western blotting analysis using antibodies of CP47 (PSII), PsaC (PSI) and IsiA and proteomic analysis on an excised protein band at ~37 kDa, the heaviest fraction (−F6) isolated from iron-deficient culture contained Chl d-bound PSI–IsiA supercomplexes. The PSII-antenna supercomplexes isolated from iron-replete conditions showed two fluorescence peaks of 712 and 728 nm, which can be assigned as 6-transmembrane helix chlorophyll-binding antenna and photosystem II fluorescence, respectively, which is supported by protein analysis of the fractions (F5 and F6). © 2017, Springer Science+Business Media Dordrecht.
语种：
英文
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UV-B induced biosynthesis of a novel sunscreen compound in solar radiation and desiccation tolerant cyanobacteria
作者：
Shang, Jin-Long;Zhang, Zhong-Chun;Yin, Xiao-Yue;Chen, Min;Hao, Fu-Hua;...
期刊：
Environmental Microbiology ,2018年20(1):200-213 ISSN：1462-2912
通讯作者：
Qiu, Bao-Sheng
（ 作者机构：
[Qiu, Bao-Sheng; Shang, Jin-Long; Yin, Xiao-Yue; Feng, Jun-Li; Xu, Hai-Feng; Wang, Kai; Yin, Yan-Chao; Zhang, Zhong-Chun] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Chen, Min] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.;[Hao, Fu-Hua] Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China.;[Tang, Hui-Ru] Fudan Univ, State Key Lab Genet Engn, Sch Life Sci, Shanghai 200438, Peoples R China.;[Tang, Hui-Ru] Fudan Univ, Zhongshan Hosp, Collaborat Innovat Ctr Genet & Dev, Minist Educ,Key Lab Contemporary Anthropol Metabo, Shanghai 200438, Peoples R China.
通讯机构：
[Qiu, Bao-Sheng] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
摘要：
The small-molecule sunscreen compounds, mycosporine-like amino acids (MAAs), have strong ultraviolet (UV) absorption and can protect cyanobacteria against UV-B damage. However, the molecular mechanism underlying UV-B signaling and MAA chemical diversity remain largely unclear. Here, we identified a five-gene cluster for MAA biosynthesis in the solar radiation and desiccation tolerant cyanobacterium Nostoc flagelliforme. A LuxR family protein OrrA was identified as a positive UV-B responsive regulator binding to the promoter region of this gene cluster. OrrA functions as an activator mediating the UV-B induced MAA biosynthesis. Overexpression of orrA strengthened its UV-B tolerance during desiccation, and enhanced the photosynthetic recovery upon rehydration. Heterologous expression of this gene cluster in Anabaena PCC 7120 produces the same MAA as that in field samples of N. flagelliforme. The MAA structure is assigned as mycosporine-2-(4-deoxygadusolyl-ornithine) with a molecular weight of 756 Da, the structurally unique MAA compound reported to date. This MAA was catalyzed by mysD-mysC2-mysC1 encoding proteins from 4-deoxygadusol, which was synthesized through the catalysis of mysA-mysB products. Thus, we elucidated the transcriptional mechanism for a novel type MAA biosynthesis in solar radiation and desiccation tolerant cyanobacteria, which shed light on the identification of other components for UV-B signaling in cyanobacteria. © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd
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英文
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Nickel and cadmium-induced SLBP depletion: A potential pathway to metal mediated cellular transformation
作者：
Jordan, Ashley;Zhang, Xiaoru;Li, Jinquan;Laulicht-Glick, Freda;Sun, Hong;...
期刊：
PLOS ONE ,2017年12(3):e0173624 ISSN：1932-6203
通讯作者：
Costa, Max
作者机构：
[Sun, Hong; Jordan, Ashley; Zhang, Xiaoru; Laulicht-Glick, Freda; Costa, Max] NYU, Sch Med, Dept Environm Med, Tuxedo Pk, NY 10987 USA.;[Jordan, Ashley; Costa, Max] NYU, Sch Med, Dept Mol Pharmacol & Biochem, New York, NY USA.;[Li, Jinquan] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Costa, Max] N;NYU, Sch Med, Dept Environm Med, Tuxedo Pk, NY 10987 USA.;NYU, Sch Med, Dept Mol Pharmacol & Biochem, New York, NY USA.
关键词：
Nickel;Histones;Cadmium;Messenger RNA;Epigenetics;Synthesis phase;DNA methylation;Arsenic
摘要：
Both nickel and cadmium compounds have been established as group I carcinogens for several decades. Despite over-whelming evidence of these compounds' carcinogenicity in humans, the specific underlying molecular mechanisms that govern metal induced cellular transformation remain unclear. In this study, we found that there were slightly different effects on decreased SLBP mRNA and protein as well as increased polyA H3.1 in our nickel exposed cells. This suggested that nickel and arsenic have similar effects on canonical histone mRNA transcription and translation. We also saw that the depletion of SLBP protein was reversed by inhibiting the proteosome. Finally, we showed that inhibiting the SLBP mRNA and protein levels were rescued by epigenetic modifiers suggesting that nickel's effects on SLBP may be mediated via epigenetic mechanisms. Taken together these results suggest a similar mechanism by which both arsenic and nickel may exert their carcinogenic effects.
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英文
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Bisphenol A Removal by Submerged Macrophytes and the Contribution of Epiphytic Microorganisms to the Removal Process
作者：
Zhang, Guosen;Wang, Yu;Jiang, Jinhui* ;Yang, Shao*
期刊：
Bulletin of Environmental Contamination and Toxicology ,2017年98(6):770-775 ISSN：0007-4861
通讯作者：
Jiang, Jinhui;Yang, Shao
作者机构：
[Yang, Shao; Jiang, JH; Yang, S; Wang, Yu; Jiang, Jinhui; Zhang, Guosen] Cent China Normal Univ, Sch Life Sci, 152 Luoyu Ave, Wuhan 430079, Hubei Province, Peoples R China.
通讯机构：
[Jiang, JH; Yang, S] C;Cent China Normal Univ, Sch Life Sci, 152 Luoyu Ave, Wuhan 430079, Hubei Province, Peoples R China.
关键词：
Macrophyte;Bisphenol A;Ceratophyllum demersum;Epiphytic microorganisms;Biodegradation
摘要：
Bisphenol A (BPA), a typical endocrine disruptor, has been found in global aquatic environments, causing great concern. The capabilities of five common submerged macrophytes to remove BPA from water and the contributions of epiphytic microorganisms were investigated. Macrophytes removed 62%–100% of total BPA (5mg/L) over 12 days; much higher rates than that observed in the control (2%, F = 261.511, p = 0.000). Ceratophyllum demersum was the most efficient species. C. demersum samples from lakes with different water qualities showed no significant differences in BPA removal rates. Moreover, removal, inhibition or re-colonization of epiphytic microorganisms did not significantly change the BPA removal rates of C. demersum. Therefore, the contributions of epiphytic microorganisms to the BPA removal process were negligible. The rate of BPA accumulation in C. demersum was 0.1%, indicating that BPA was mainly biodegraded by the macrophyte. Hence, submerged macrophytes, rather than epiphytic microorganisms, substantially contribute to the biodegradation of BPA in water. © 2017, Springer Science+Business Media New York.
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英文
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The roles of call wall invertase inhibitor in regulating chilling tolerance in tomato
作者：
Xu, Xiao-xia;Hu, Qin;Yang, Wan-nian;Jin, Ye*
期刊：
BMC Plant Biology ,2017年17(1):1-13 ISSN：1471-2229
通讯作者：
Jin, Ye
作者机构：
[Xu, Xiao-xia; Jin, Ye; Hu, Qin; Yang, Wan-nian] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Jin, Ye] C;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
关键词：
Abscisic acid;Cell wall invertase inhibitor;Chilling tolerance;C-repeat binding factors;Solanum lycopersicum;Sugar signaling
摘要：
Background: Hexoses are important metabolic signals that respond to abiotic and biotic stresses. Cold stress adversely affects plant growth and development, limiting productivity. The mechanism by which sugars regulate plant cold tolerance remains elusive. Results: We examined the function of INVINH1, a cell wall invertase inhibitor, in tomato chilling tolerance. Cold stress suppressed the transcription of INVINH1 and increased that of cell wall invertase genes, Lin6 and Lin8 in tomato seedlings. Silencing INVINH1 expression in tomato increased cell wall invertase activity and enhanced chilling tolerance. Conversely, transgenic tomatoes over-expressing INVINH1 showed reduced cell wall invertase activity and were more sensitive to cold stress. Chilling stress increased glucose and fructose levels, and the hexoses content increased or decreased by silencing or overexpression INVINH1. Glucose applied in vitro masked the differences in chilling tolerance of tomato caused by the different expressions of INVINH1. The repression of INVINH1 or glucose applied in vitro regulated the expression of C-repeat binding factors (CBFs) genes. Transcript levels of NCED1, which encodes 9-cisepoxycarotenoid dioxygenase (NCED), a key enzyme in the biosynthesis of abscisic acid, were suppressed by INVINH1 after exposure to chilling stress. Meanwhile, application of ABA protected plant from chilling damage caused by the different expression of INVINH1. Conclusions: In tomato, INVINH1 plays an important role in chilling tolerance by adjusting the content of glucose and expression of CBFs. © 2017 The Author(s).
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英文
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