摘要：
目的:探讨超氧化物歧化酶模型化合物(models of superoxide dismutase,MSOD)对离体人肝癌细胞毒作用.方法:采用离体培养的人肝癌细胞SSMC-7721细胞株,常规方法接种入24孔板,加入SOD模型化合物,以彗星实验为终点效应,检测其对肿瘤细胞的影响.结果:通过彗星试验检测,各剂量组所起的肝癌细胞DNA损伤或DNA断裂的程度呈明显的剂量效应关系.结论:这种模型化合物对离体培养的肝癌细胞有明显细胞毒作用.
作者机构：
[Deng, HW] Creighton Univ, Osteoporosis Res Ctr, Omaha, NE 68131 USA.;Creighton Univ, Dept Biomed Sci, Omaha, NE 68131 USA.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;Hunan Normal Univ, Coll Life Sci, Lab Mol & Stat Genet, Changsha 410081, Hunan, Peoples R China.;Yale Univ, Sch Med, Ctr Med Informat, New Haven, CT 06520 USA.
通讯机构：
[Deng, HW] C;Creighton Univ, Osteoporosis Res Ctr, Omaha, NE 68131 USA.
会议名称：
2nd International Conference on Osteoporosis in Men
会议时间：
APR 03-05, 2003
会议地点：
GENOA, ITALY
会议主办单位：
Creighton Univ, Osteoporosis Res Ctr, Omaha, NE 68131 USA.^Creighton Univ, Dept Biomed Sci, Omaha, NE 68131 USA.^Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.^Hunan Normal Univ, Coll Life Sci, Lab Mol & Stat Genet, Changsha 410081, Hunan, Peoples R China.^Yale Univ, Sch Med, Ctr Med Informat, New Haven, CT 06520 USA.
关键词：
Bone mineral density;Genome scan;Linkage;Osteoporosis
摘要：
Low bone mineral density (BMD) is a major risk factor for osteoporotic fracture. To identify genomic regions harboring quantitative trait loci (QTLs) contributing to BMD variation, we performed a two-stage genome screen. The first stage involved genotyping of a sample of 53 pedigrees with 630 individuals using 400 microsatellite markers spaced at approximately 10-cM intervals throughout the genome. Ten genomic regions with multi- and/or two-point LOD scores greater than 1.5 were observed. In the present second-stage study, 60 microsatellite markers, with a mean spacing of about 5 cM, were genotyped in these regions in an expanded sample of 79 pedigrees that contained 1816 subjects. Each pedigree was ascertained through a proband with extreme BMD at the hip or spine. BMD at the spine (L1-4), hip (the femoral neck, trochanter, and intertrochanteric region), and wrist (the ultradistal region) was measured by dual-energy X-ray absorptiometry (DXA) and was adjusted for age, sex, height, and weight. Two-point and multipoint linkage analyses were performed for each BMD site using statistical genetic methods that are implemented in the computer package SOLAR. Several regions (7q11, 10q26, 12q13, and 12q24) achieved LOD scores in excess of 1 in the second-stage followup study. The current results replicate some of our previous linkage findings and also highlight some of the difficulties facing microsatellite linkage mapping for complex human diseases.
作者机构：
[吴飞健; 陈其才] 华中师范大学生命科学学院;[Philip H. S. Jen] Division of Biological Sciences, University of Missouri-Columbia, Columbia, MO 65211,USA;[沈钧贤] 中国科学院生物物理研究所
