作者： Li, Zheng-Ke;Yin, Yan-Chao;Zhang, Lu-Dan;Zhang, Zhong-Chun;Dai, Guo-Zheng;...

期刊： Photosynthesis Research,2018年135(1-3):165-175 ISSN：0166-8595

通讯作者： Qiu, Bao-Sheng;Chen, Min

作者机构： [Qiu, Bao-Sheng; Zhang, Lu-Dan; Li, Zheng-Ke; Dai, Guo-Zheng; Yin, Yan-Chao; Zhang, Zhong-Chun] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Qiu, Bao-Sheng; Zhang, Lu-Dan; Li, Zheng-Ke; Dai, Guo-Zheng; Yin, Yan-Chao; Zhang, Zhong-Chun] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Chen, Min] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.

通讯机构： [Qiu, Bao-Sheng] C;[Chen, Min] U;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.

会议名称： Light Harvesting Satellite Meeting held in conjunction with the 17th International Congress on Photosynthesis Research

会议时间： AUG, 2016

会议地点： Egmond aan Zee, NETHERLANDS

会议主办单位： [Li, Zheng-Ke;Yin, Yan-Chao;Zhang, Lu-Dan;Zhang, Zhong-Chun;Dai, Guo-Zheng;Qiu, Bao-Sheng] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.^[Li, Zheng-Ke;Yin, Yan-Chao;Zhang, Lu-Dan;Zhang, Zhong-Chun;Dai, Guo-Zheng;Qiu, Bao-Sheng] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.^[Chen, Min] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.

关键词： Accessory chlorophyll-binding proteins (CBPs);Iron limitation;Iron-stress-induced protein A (IsiA);Photosynthesis;Six transmembrane helix family of chlorophyll-binding proteins/antenna;Acaryochloris marina

摘要： The bioavailable iron in many aquatic ecosystems is extremely low, and limits the growth and photosynthetic activity of phytoplankton. In response to iron limitation, a group of chlorophyll-binding proteins known as iron stress-induced proteins are induced and serve as accessory light-harvesting components for photosystems under iron limitation. In the present study, we investigated physiological features of Acaryochloris marina in response to iron-deficient conditions. The growth doubling time under iron-deficient conditions was prolonged to ~3.4 days compared with 1.9 days under normal culture conditions, accompanied with dramatically decreased chlorophyll content. The isolation of chlorophyll-binding protein complexes using sucrose density gradient centrifugation shows six main green bands and three main fluorescence components of 712, 728, and 748 nm from the iron-deficient culture. The fluorescence components of 712 and 728 nm co-exist in the samples collected from iron-deficient and iron-replete cultures and are attributed to Chl d-binding accessory chlorophyll-binding antenna proteins and also from photosystem II. A new chlorophyll-binding protein complex with its main fluorescence peak at 748 nm was observed and enriched in the heaviest fraction from the samples collected from the iron-deficient culture only. Combining western blotting analysis using antibodies of CP47 (PSII), PsaC (PSI) and IsiA and proteomic analysis on an excised protein band at ~37 kDa, the heaviest fraction (−F6) isolated from iron-deficient culture contained Chl d-bound PSI–IsiA supercomplexes. The PSII-antenna supercomplexes isolated from iron-replete conditions showed two fluorescence peaks of 712 and 728 nm, which can be assigned as 6-transmembrane helix chlorophyll-binding antenna and photosystem II fluorescence, respectively, which is supported by protein analysis of the fractions (F5 and F6). © 2017, Springer Science+Business Media Dordrecht.

语种： 英文