摘要：
Diverse stimuli induce stomatal closure by triggering the efflux of osmotic anions, which is mainly mediated by the main anion channel SLAC1 in plants, and the anion permeability and selectivity of SLAC1 channels from several plant species have been reported to be variable. However, the genetic identity as well as the anion permeability and selectivity of the main S-type anion channel ZmSLAC1 in maize are still unknown. In this study, we identified GRMZM2G106921 as the gene encoding ZmSLAC1 in maize, and the maize mutants zmslac1-1 and zmslac1-2 harboring a mutator (Mu) transposon in ZmSLAC1 exhibited strong insensitive phenotypes of stomatal closure in response to diverse stimuli. We further found that ZmSLAC1 functions as a nitrate-selective anion channel without obvious permeability to chloride, sulfate and malate, clearly different from SLAC1 channels of Arabidopsis thaliana, Brassica rapa ssp. chinensis and Solanum lycopersicum L. Further experimental data show that the expression of ZmSLAC1 successfully rescued the stomatal movement phenotypes of the Arabidopsis double mutant atslac1-3atslah3-2 by mainly restoring nitrate-carried anion channel currents of guard cells. Together, these findings demonstrate that ZmSLAC1 is involved in stomatal closure mainly by mediating the efflux of nitrate in maize.
期刊：
Journal of Bacteriology,2018年200(21):JB.00436-18 ISSN：0021-9193
通讯作者：
Qiu, Bao Sheri
作者机构：
[Zang, Sha-Sha; Li, Zheng-Ke; Qiu, Bao Sheri; Dai, Guo-Zheng; Liu, Ke; Song, Wei-Yu] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.;[Zang, Sha-Sha; Li, Zheng-Ke; Qiu, Bao Sheri; Dai, Guo-Zheng; Liu, Ke; Song, Wei-Yu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.;[Chen, Min] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW, Australia.
通讯机构：
[Qiu, Bao Sheri] C;Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.
作者：
Zhang, Zhongchun;Xie, Qingqing;Jobe, Timothy O.;Kau, Andrew R.;Wang, Cun;...
期刊：
分子植物,2016年9(3):481-484 ISSN：1674-2052
通讯作者：
Julian I. Schroeder
作者机构：
[Jobe, Timothy O.; Kau, Andrew R.; Wang, Cun; Schroeder, Julian I.] Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, La Jolla, CA 92093-0116, USA;School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, P.R. China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan 430079, Hubei, P.R. China;[Wang, Qiuquan] Department of Chemistry & the MOE Key Lab of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, 361005, China;[DavidG. Mendoza-Cózatl] Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA
通讯机构：
[JulianI. Schroeder] D;Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, La Jolla, CA 92093-0116, USA
关键词：
detoxification;Cadmium;phytochelatins
摘要：
Glutathione (GSH) is an essential tri-peptide important for several plant processes, including the control of cellular redox status, detoxification of xenobiotics, root development, heavy metal transport and resistance, and long-distance transport of organic sulfur.Cadmium and arsenic are detoxified by small GSH-derived peptides, called phytochelatins (PCs). Phytochelatins are rapidly synthesized in response to toxic metal exposure and have long been known to play an integral role in metal(loid) detoxification in plants. However, while phytochelatins are largely synthesized in roots, GSH is mainly synthesized in chloroplasts and transported in the phloem (Wachter et al., 2005; Mendoza-Cozatl et al., 2008). Thus, GSH transport from shoots to roots is critical for phytochelatin synthesis in plants. However, genes encoding plasma membrane GSH-specific transporters remain largely unknown (Zechmann, 2014; Pike et al., 2009; Cagnac et al., 2004).
作者机构：
[AI; GAO; QIU; CHEN; GONG] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, 430079, China
通讯机构：
[Xiang Gao] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China
关键词：
heavy metals;hyperaccummulator;lake water;phytoremediation;Sedum alfredii
摘要：
Sedum alfredii Hance is a terrestrial zinc/cadmium (Zn/Cd)-hyperaccumulating and lead (Pb)-accumulating plant. Previous studies on S. alfredii were mostly focused on its physiological mechanism of heavy metal uptake and the application in phytoextraction of metals from contaminated soils. In this study, we evaluated the application potential of S. alfredii in the cleanup of heavy metals from contaminated lake water. Our research revealed that changing pH in lake water would not make particular difference on the final accumulation amount of heavy metals, because the acidic water environment negatively affected plant growth compared with the neutral and alkaline environments, but was more conducive for heavy metal absorption and accumulation. In addition, S. alfredii showed an increase of approximately 2.2-fold in dry weight (DW) when cultured with lake water for 25 d. At the same time, it accumulated approximately 5.0 mg/kg DWof Cd and 41.4 mg/kg DWof Pb. The absorption of heavy metals was highly effective during the first 10 d of culture. Also, the quality of lake water was greatly improved after only 2-d cleanup by S. alfredii. In general, this hyperaccumulator exhibits great potential for application in the cleanup of heavy metals-polluted waters.
