核心素养导向的基于思维导图的分析化学教学实践与探讨
作者：
龚静鸣;张文华;李芳;徐晖;周燕平;...
期刊：
大学化学 ,2019年34(6):26-32 ISSN：1000-8438
作者机构：
华中师范大学化学学院,武汉,430079;[万坚; 高婷娟; 龚静鸣; 熊博; 徐晖; 原弘; 李芳; 梁沛; 周燕平; 张文华] 华中师范大学
关键词：
核心素养;思维导图;分析化学
摘要：
运用“思维导图”这一教学策略,引导学生在学习过程中自发有效地对各知识点进行关联,构建完整的知识网络,进而改变学生单纯依靠“机械式”记忆的学习方法,锤炼其对知识点的凝练总结能力,培养学生的自主学习能力,实现基于核心素养培养观导向的以培养具有良好分析化学素养,具备竞争力和创新能力的高端人才培养目标。
语种：
中文
展开
High Performance Soluble Polyimides from Ladder-Type Fluorinated Dianhydride with Polymorphism
作者：
Li, Fu;Liu, Jikang;Liu, Xiangfu;Wang, Yao;Gao, Xiang;...
期刊：
Polymers ,2018年10(5):546- ISSN：2073-4360
通讯作者：
Tu, Guoli;Meng, Xianggao
作者机构：
[Wang, Yao; Liu, Xiangfu; Tu, Guoli; Li, Fu; Liu, Jikang] Huazhong Univ Sci & Technol, Wuhan Natl Res Ctr Optoelect, Wuhan 430074, Hubei, Peoples R China.;[Gao, Xiang] Wuhan Inst Technol, Sch Mat Sci & Engn, Wuhan 403052, Hubei, Peoples R China.;[Meng, Xianggao] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Tu, Guoli] H;[Meng, Xianggao] C;Huazhong Univ Sci & Technol, Wuhan Natl Res Ctr Optoelect, Wuhan 430074, Hubei, Peoples R China.;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Hubei, Peoples R China.
关键词：
dianhydride;semi-alicyclic;polymorphic;coefficient of thermal expansion;polyimide
摘要：
A novel rigid semi-alicyclic dianhydride 9,10-difluoro-9,10-bis(trifluoromethyl)-9,10 -dihydroanthracene-2,3,6,7-tetracarboxylic acid dianhydride (8FDA) was reported, and its single crystal X-ray diffraction result revealed the existence of the polymorphic structure in this compound. The detail geometric configuration transition during the synthesized process was investigated, exhibiting a transition of from trans- to cis- when the hydroxyl groups were substituted by fluoride with diethylaminosulfur trifluoride (DAST). Compared with the dianhydride 4,4'-(Hexaflouroisopropylidene) diphthalic anhydride (6FDA) and 1S,2R,4S,5R-cyclohexanetetracarboxylic dianhydride (HPMDA), the resulting polyimide (PI) films based on 8FDA exhibited an obviously higher glass transition temperature (T<inf>g</inf>, 401 °C) and a much lower coefficient of thermal expansion (CTE, 14 ppm K<sup>-1</sup>). This indicates that 8FDA is an ideal building block in high-performance soluble PIs with low CTE.<br/> ©2018 by the authors.
语种：
英文
展开
Family-Wide Characterization of Histone Binding Abilities of PHD Domains of AL Proteins in Arabidopsis thaliana
作者：
Liang, Xiao;Lei, Ming;Li, Fangzhou;Yang, Xiajie;Zhou, Mengqi;...
期刊：
PROTEIN JOURNAL ,2018年37(6):531-538 ISSN：1572-3887
通讯作者：
Liu, Yanli
作者机构：
[Liu, Jinlin; Liang, Xiao; Yang, Xiajie; Gong, Siying; Li, Fangzhou; Qi, Chao; Lei, Ming; Liu, Ke; Li, Bing; Liu, Yanli; Zhou, Mengqi] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Cao, Yu] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Liu, Yanli] C;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
关键词：
AL protein;PHD domain;Histone binding
摘要：
Alfin1-like (AL) is a family of proteins homologous to the alfalfa Alfin1 in plant and bears an Alfin domain and a PHD domain at their N- and C-terminus, respectively. There are 7 AL proteins in Arabidopsis, and the PHD domains of most AL proteins are reported to bind to histone H3K4me3. Here we reported gene cloning, protein expression and purification of the PHD domains of all the AL family proteins in Arabidopsis. We then systematically characterized their histone binding abilities by quantitative isothermal titration calorimetry and fluorescence polarization binding assays. Our binding results indicate that all the PHD domains of the AL proteins bind to the histone H3K4me3 peptide with varying methylation state preference and binding affinities. Our study presented here provides the foundation for further studies of the peptide state-specific recognition by PHD domains of AL proteins.
语种：
英文
展开
Baseline susceptibility and cross-resistance of cycloxaprid, a novel cis-nitromethylene neonicotinoid insecticide, in Bemisia tabaci MED from China
作者：
Wang, Ran;Fang, Yong;Mu, Changqing;Qu, Cheng;Li, Fengqi;...
期刊：
Crop Protection ,2018年110:283-287 ISSN：0261-2194
通讯作者：
Luo, Chen
作者机构：
[Li, Fengqi; Wang, Ran; Luo, Chen; Qu, Cheng] Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China.;[Fang, Yong] Hunan Acad Agr Sci, Plant Protect Inst, Changsha 410125, Hunan, Peoples R China.;[Mu, Changqing] Beijing Stn Plant Protect, Beijing 100029, Peoples R China.;[Wang, Zhenyu] Cent China Normal Univ, Coll Chem, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Luo, Chen] B;Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China.
关键词：
Baseline susceptibility;Bemisia tabaci;Cross-resistance;Cycloxaprid;Neonicotinoids
摘要：
The tobacco whitefly Bemisia tabaci (Gennadius) is an important crop pest which threatens agriculture worldwide. It has evolved resistance to various classes of insecticides. Cycloxaprid is a new-generation neonicotinoid which is a (nitromethylene) imidazole analogue of imidacloprid which is highly efficient for the control of various Hemipteran pests in China. Studies were conducted to determine the baseline susceptibility to cycloxaprid of 18 field samples of B. tabaci collected from 9 geographical locations in China and the possibility of cross-resistance between cycloxaprid and other important neonicotinoids of one laboratory-selected resistant strain. The 50% lethal concentrations (LC50) of cycloxaprid to these 18 samples ranged from 0.84 to 12.17 mg/L. Furthermore, compared with the susceptible laboratory strain, the imidacloprid-resistant strain exhibited a 27.9-fold resistance to imidacloprid and lower level of cross-resistance to acetamiprid (16.3-fold), thiacloprid (13.7-fold) and nitenpyram (16.6-fold), but no cross-resistance to cycloxaprid (1.9-fold). These results demonstrate that cycloxaprid could be one effective alternative insecticide for whitefly management that could reduce imidacloprid selection pressure.
语种：
英文
展开
瓜蒌薤白复方抗急性心肌缺血的代谢组学研究
作者：
李迎浩;徐倩;闫换玲;何晓敏;张丽萍;...
作者机构：
[李迎浩; 徐倩; 闫换玲; 何晓敏; 张丽萍; 刘小鹏; 李芳] 华中师范大学化学学院农药与化学生物学教育部重点实验室
会议名称：
第十九届全国波谱学学术会议
会议时间：
2016-08-17
会议地点：
中国甘肃兰州
摘要：
<正>近年来,心肌缺血在许多国家和地区呈现出高发病率和高死亡率,严重影响着人类生活与健康[1]。在中医学中,心肌缺血属于"胸痹’’范畴,瓜蒌薤白复方是主治胸痹的驰名古方[2]。现代药理研究表明,单味瓜蒌、薤白对缺血心肌均有一定的保护作用,瓜蒌薤白复方对缺血心肌有显著的协同保护作用[3],然而其协同作用机制尚不清楚。本文利用核磁共振技术结合多变量数据分析方法研究瓜蒌薤白复方对急性心
语种：
中文
展开
Polymer monolithic column containing embedded graphene oxide sheets for sensitive determination of carbamate insecticides by HPLC (vol 183, pg 543, 2016)
作者：
Jin, Tingting;Li, Feng;Cheng, Jing* ;Wu, Shiju;Zhou, Hongbin;...
期刊：
Microchimica Acta ,2016年183(2):553-553 ISSN：0026-3672
通讯作者：
Cheng, Jing;Cheng, Min
作者机构：
[Wu, Shiju; Li, Feng; Zhou, Hongbin; Jin, Tingting; Cheng, Jing] Cent China Normal Univ, Coll Chem, Inst Environm Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.;[Cheng, Min] Huazhong Univ Sci & Technol, Sch Mech Sci & Engn, Wuhan 430074, Peoples R China.
通讯机构：
[Cheng, Jing] C;[Cheng, Min] H;Cent China Normal Univ, Coll Chem, Inst Environm Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.;Huazhong Univ Sci & Technol, Sch Mech Sci & Engn, Wuhan 430074, Peoples R China.
摘要：
We describe a new kind of polymer monolithic column for polymer monolith microextraction (PMME) of carbamate insecticide (methomyl, carbofuran and pirimicarb) residues from water samples. The monolith was prepared inside the tip of a micropipette via thermally initiated free-radical polymerization of methacrylic acid as the monomer, ethylene glycol dimethacrylate as the cross-linker, and graphene oxide (GO) dispersed in the mixed porogens (1-propanol and 1,4-butanediol). The resulting monolith was characterized by scanning electron microscopy and IR spectrometry. The effects of sample flow rate, sample volume, sample pH, desorption solvent and flow rate on PMME were optimized. Compared to a monolith without GO, the new monolith displays higher enrichment capacity, and is reusable and stable. Following extraction, the three carbamates were quantified by HPLC. The limits of detection (for S/N = 3) forthe three carbamates are in the range of 0.3–0.7μg∙L −1 , and the intra- and inter-day precisions are <5.7%. The new monolith was successfully applied for the determination of the insecticides in various (spiked) environmental water samples, with recoveries in the range from 78.9 to 103.4%. © 2015, Springer-Verlag Wien.
语种：
英文
展开
Extraction of endocrine disrupting phenols with iron-ferric oxide core-shell nanowires on graphene oxide nanosheets, followed by their determination by HPLC
作者：
Li, Feng;Cai, Cuicui;Cheng, Jing* ;Zhou, Hongbin;Ding, Kerong;...
期刊：
Microchimica Acta ,2015年182(15-16):2503-2511 ISSN：0026-3672
通讯作者：
Cheng, Jing
作者机构：
[Zhang, Lizhi; Li, Feng; Zhou, Hongbin; Cheng, Jing; Ding, Kerong; Cai, Cuicui] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Cheng, Jing] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Iron-ferric oxide/graphene oxide composite;Magnetic solid-phase extraction;Magnetic sorbent;Scanning electron microscopy;Transmission electron microscopy;High performance liquid chromatography;Environmental analysis
摘要：
We describe a novel magnetic nanosorbent that consists of nanowires consisting of a core of metallic iron and an iron (III) oxide shell. These nanowires were then deposited on graphene oxide to form a composite of the type Fe@Fe2O3/GO. Specifically, the magnetic composite is formed via electrostatic interaction between negatively charged GO nanosheets and positively charged Fe@Fe2O3 nanowires in aqueous solution. The material was successfully applied to the extraction of the endocrine-disrupting phenols bisphenol A, triclosan and2,4-dichlorophenol from water samples. Compared to neat graphene oxide, the composite material exhibits improved properties in terms of microextraction where both the hydrophilic graphene oxide and the Fe@Fe2O3 nanowires participate in the adsorption of the hydrophilic analytes. The amount of adsorbent, pH of water sample, extraction time and desorption time, type and volume of desorption solution were optimized. Following extraction for the absorbent, the phenols were quantified by HPLC. The three phenols can be determined in 0.5 to 100ng∙mL−1 concentration range, with limits of detection (at an S/N ratio of 3) ranging from 0.08 to 0.10ng∙mL−1. The repeatability was investigated by evaluating the intra- and inter-day precisions with relative standard deviations of lower than 7.5% (n = 5). The recoveries from spiked real water samples were in the range from 84.8 to 92.0%. The results indicate that the novel material can be successfully applied to the extraction and analysis of phenols from water samples. [Figure not available: see fulltext.] © 2015, Springer-Verlag Wien.
语种：
英文
展开
Polyaniline-coated chitosan-functionalized magnetic nanoparticles: Preparation for the extraction and analysis of endocrine-disrupting phenols in environmental water and juice samples
作者：
Jiang, Xilan;Cheng, Jing* ;Zhou, Hongbin;Li, Feng;Wu, Wenlin;...
期刊：
Talanta ,2015年141:239-246 ISSN：0039-9140
通讯作者：
Cheng, Jing
作者机构：
[Zhou, Hongbin; Li, Feng; Cheng, Jing; Jiang, Xilan; Wu, Wenlin; Ding, Kerong] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Inst Environm Chem,Coll Chem, Wuhan 430079, Peoples R China.
通讯机构：
[Cheng, Jing] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Inst Environm Chem,Coll Chem, Wuhan 430079, Peoples R China.
关键词：
Environmental water and juice samples;Fe(3)O(4)@chitosan@polyaniline magnetic nanoparticles;High performance liquid chromatography;Magentic solid phase extraction;Phenols
摘要：
In the present study, chitosan (CHI) functionalized Fe<inf>3</inf>O<inf>4</inf>magnetic microspheres coated with polyaniline (PANI) were synthesized for the first time. The chitosan-functionalized magnetic microspheres (Fe<inf>3</inf>O<inf>4</inf>@CHI) were synthesized by a co-precipitation method, and then aniline was polymerized on the magnetic core. The obtained Fe<inf>3</inf>O<inf>4</inf>@CHI@PANI microspheres were spherical core-shell structure with uniform size at about 100 nm with 20-30 nm diameter core. The microspheres had a high saturation magnetization of 32 emu g<sup>-</sup><sup>1</sup>, which was sufficient for magnetic separation. The obtained Fe<inf>3</inf>O<inf>4</inf>@CHI@PANI magnetic microspheres were applied as magnetic adsorbents for the extraction of aromatic compounds via π-πinteraction between polyaniline shell and aromatic compounds. Three endocrine-disrupting phenols, including bisphenol A (BPA), 2, 4-dichlorophenol (2, 4-DCP), and triclosan (TCS) were selected as the model analytes to verify the extraction ability of Fe<inf>3</inf>O<inf>4</inf>@CHI@PANI. The hydrophilic chitosan-functionalized Fe<inf>3</inf>O<inf>4</inf>core (Fe<inf>3</inf>O<inf>4</inf>@CHI) improved the dispersibility of Fe<inf>3</inf>O<inf>4</inf>@CHI@PANI microspheres, and then improve its extraction efficiency. The dominant parameters affecting enrichment efficiency were investigated and optimized. Under optimal condition, the proposed method was evaluated, and applied to the analysis of phenols in real water and juice samples. The results demonstrated the method based on Fe<inf>3</inf>O<inf>4</inf>@CHI@PANI magnetic microspheres had good linearity (R<sup>2</sup>>0.996), and limits of detection (0.10-0.13 ng mL<sup>-1</sup>), high repeatability (RSD<6.6%) and good recovery (85.0-106.7%). ©2015 Elsevier B.V. All rights reserved.
语种：
英文
展开
Metabonomic analysis of Allium macrostemon Bunge as a treatment for acute myocardial ischemia in rats
作者：
Li, Fang* ;Xu, Qian;Zheng, Ting;Huang, Fang;Han, Lintao
期刊：
Journal of Pharmaceutical and Biomedical Analysis ,2014年88:225-234 ISSN：0731-7085
通讯作者：
Li, Fang
作者机构：
[Li, Fang; Xu, Qian; Zheng, Ting] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticides & Chem Biol, Wuhan 430079, Hubei, Peoples R China.;[Huang, Fang; Han, Lintao] Hubei Univ Chinese Med, Minist Educ, Key Lab Chinese Med Resource & Compound Prescript, Wuchang, Peoples R China.
通讯机构：
[Li, Fang] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticides & Chem Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
amino acid;carbohydrate;carboxylic acid;Allium;Allium macrostemon Bunge;amino acid metabolism;animal experiment;animal model;article;biomics;discriminant analysis;energy metabolism;glycolysis;heart muscle ischemia;metabolic balance;metabolite;metabonomics;nonhuman;nuclear magnetic resonance spectroscopy;orthogonal projection to latent structures discriminate analysis;principal component analysis;priority journal;proton nuclear magnetic resonance;rat;Acute myocardial ischemia;Allium macrostemon Bunge;Metabonomics;Multivariate statistical analysis;NMR;Adenosine Triphosphate;Allium;Amino Acids;Animals;Biological Markers;Energy Metabolism;Glycolysis;Heart;Magnetic Resonance Spectroscopy;Male;Medicine, Chinese Traditional;Metabolomics;Multivariate Analysis;Myocardial Ischemia;Oxygen Consumption;Plant Extracts;Principal Component Analysis;Rats;Rats, Sprague-Dawley
摘要：
Myocardial ischemia (MI) refers to a pathological state of the heart caused by reduced cardiac blood perfusion, which leads to a decreased oxygen supply in the heart and an abnormal myocardial energy metabolism. Acute myocardial ischemia (AMI) has posed a significant health risk for humans. Allium macrostemon Bunge (AMB), a popular traditional Chinese medicine, is used for MI treatment. The therapeutic effects of AMB were assessed and the detailed mechanisms of AMB for AMI treatment were investigated. We characterized the metabonomic variations in rats from the sham surgery, AMI, and AMB-pretreated AMI groups through a combination of nuclear magnetic resonance (NMR) spectroscopy and multivariate statistical analysis. Thirty-five metabolites including carbohydrates, a range of amino acids, and organic acids were detected. The H-1 NMR spectra of the rat serum were analyzed using the principal component analysis (PCA) and orthogonal projection to latent structures discriminate analysis (OPLS-DA). Results showed that AMI induced some physiological changes in rats and also led to metabolic disorders related to glycolysis promotion, amino acid metabolism disruption, and other metabolite metabolism perturbation. AMB pretreatment reduced the AMI injury and maintained metabolic balance, possibly by limiting the change in energy metabolism and regulating amino acid metabolism. These findings provide a comprehensive insight on the metabolic response of AMI rats to AMB pretreatment and are important for the use of AMB for AMI therapy. Published by Elsevier B.V.
语种：
英文
展开
NMR spectroscopy and multivariate statistical analysis of metabonomic changes in Allium macrostemon Bunge extracts induced by different drying methods
作者：
Li, Fang* ;Zheng, Ting;Xu, Qian;Huang, Fang;Liu, Xiaopeng;...
期刊：
ANALYTICAL METHODS ,2013年5(21):6219-6227 ISSN：1759-9660
通讯作者：
Li, Fang
作者机构：
[Li, Fang; Xu, Qian; Liu, Xiaopeng; Zheng, Ting] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticides & Chem Biol, Wuhan 430079, Hubei, Peoples R China.;[Huang, Fang; Han, Lintao] Hubei Univ Chinese Med, Minist Educ, Key Lab Chinese Med Resource & Compound Prescript, Wuhan, Peoples R China.
通讯机构：
[Li, Fang] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticides & Chem Biol, Wuhan 430079, Hubei, Peoples R China.
摘要：
Phytomedicines are mostly dried, in different ways, before they are processed and used, which may induce metabolic changes and even lead to some changes in drug efficiency. In this study, we used NMR spectroscopy coupled with multivariate statistical analysis to investigate the metabolic consequences of Allium macrostemon Bunge (AMB) extracts induced by different drying methods, including freeze drying, shade drying, and sun drying. More than 30 metabolites have been detected in AMB extracts, among which two compounds have not been assigned yet. Multivariate statistical analysis showed that different drying methods gave rise to metabolite variations. As a plant osmoprotectant, the proline content was higher in three drying extracts than in the fresh extract. As plant-regulating agents, sugars and amino acids changed differently in each drying extract. Membrane degradation, putrescine formation, tricarboxylic acid cycle, gluconeogenesis and metabolisms mediated by shikimate were also influenced by drying processes. The result indicated that freeze drying may be the appropriate drying method for AMB because of the higher N-trans- feruloyltyramine content, which was a potential effective component of AMB. © 2013 The Royal Society of Chemistry.
语种：
英文
展开
高转速下采用S-RESIDOR方法测量~1H-~(27)Al偶极偶合常数
作者：
何义艳;王秀梅;李芳;陈雷
期刊：
波谱学杂志 ,2013年30(1):93-102 ISSN：1000-4556
作者机构：
华中师范大学化学学院,湖北武汉,430079;波谱与原子分子物理国家重点实验室,武汉磁共振中心(中国科学院武汉物理与数学研究所),湖北武汉430071;中国科学院大学,北京100049;[陈雷; 王秀梅] 中国科学院武汉物理与数学研究所;[李芳; 何义艳] 华中师范大学
关键词：
核磁共振(NMR);偶极偶合常数;丝光沸石
摘要：
提出利用S-RESIDOR(Symmetry-based Resonance Echo Saturation Irradiation DOubleResonance)方法测量~1H-~(27)Al偶极偶合常数.通过理论模拟,讨论了四极作用常数、饱和照射脉冲功率、照射时间对~1H/~(27)Al S-RESIDOR实验的影响.发现四极作用常数对~1H/~(27)Al S-RESIDOR偶极去相曲线影响较小,中等强度的饱和照射功率即能满足实验要求.高转速下,在氢型丝光沸石上进行了~1H/~(27)Al S-RESIDOR实验,测量得到Brnsted酸位上的~1H与骨架上~(27)Al的偶极偶合常数为874Hz.
语种：
中文
展开
Adsorption and reduction reactions of anthraquinone derivatives on gold electrodes studied with electrochemical surface-enhanced Raman spectroscopy
作者：
Dai, Ke;Huang, Rong;Jiang, Rui;Ke, Hui-Xian;Li, Fei;...
期刊：
Journal of Raman Spectroscopy ,2012年43(10):1367-1373 ISSN：0377-0486
通讯作者：
Jin, Shan
作者机构：
[Tian, Zhong-Qun; Wu, De-Yin; Dai, Ke; Huang, Rong; Ke, Hui-Xian] Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China.;[Jin, Shan; Li, Fei; Jiang, Rui] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.;[Tian, Zhong-Qun; Wu, De-Yin; Dai, Ke; Huang, Rong; Ke, Hui-Xian] Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China.
通讯机构：
[Jin, Shan] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
关键词：
anthraquinone;gold electrode;self-assembled monolayer;surface-enhanced Raman spectroscopy;density functional theory
摘要：
<jats:p>Electrochemical surface‐enhanced Raman spectroscopy (EC‐SERS), combined with cyclic voltammetry, and the density functional theoretical (DFT) method were used to investigate self‐assembled monolayer (SAM) adsorption and reduction processes. Here, we choose the system of interest, being thiolacetyl‐terminated 2‐phenylene ethynylene‐substituted anthraquinone molecule (2‐AQ) on gold electrodes in buffered aqueous and aprotic solutions. In the buffered aqueous solution, the results of cyclic voltammetry and EC‐SERS measurements, as well as DFT calculations, indicate that the adsorbed molecules pass through a two‐electron two‐proton reduction reaction with cathodic polarization. In particular, the latter two methods confirmed the structural changes of SAMs during the process of redox reaction, 2‐AQ + 2e + 2H<jats:sup>+</jats:sup> → 2‐AQH<jats:sub>2</jats:sub>, where 2‐AQ and 2‐AQH<jats:sub>2</jats:sub> are the oxidized and reduced forms, respectively. In aprotic solutions (acetonitile), a stepwise reaction mechanism was proposed on the basis of the results of EC‐SERS and DFT calculations. The first reduction peak should be a half reaction process 2‐AQ + e → 2‐AQ<jats:sup>−</jats:sup>, where 2‐AQ<jats:sup>−</jats:sup> is a single electron reduced form. Compared with that of 2‐AQ SAMs in the buffered aqueous solution, the results of EC‐SERS and DFT calculations in aprotic solution suggested that the solvent effect significantly influences the redox process of 2‐AQ in electrochemical interfaces. Copyright © 2012 John Wiley & Sons, Ltd.</jats:p>
语种：
英文
展开
Understanding the Interaction between Valsartan and Detergents by NMR Techniques and Molecular Dynamics Simulation
作者：
Cao, Chenyu;Mao, Jiezhen;Li, Fang;Yang, Minghui;He, Hongqing* ;...
期刊：
JOURNAL OF PHYSICAL CHEMISTRY B ,2012年116(25):7470-7478 ISSN：1520-6106
通讯作者：
He, Hongqing
作者机构：
[Yang, Minghui; Liu, Maili; Mao, Jiezhen; Cao, Chenyu; Jiang, Ling; He, Hongqing] Chinese Acad Sci, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China.;[Cao, Chenyu] Huazhong Univ Sci & Technol, Sch Life Sci & Technol, Wuhan 430074, Peoples R China.;[Li, Fang] Cent China Normal Univ, Dept Chem, Wuhan 430079, Peoples R China.
通讯机构：
[He, Hongqing] C;Chinese Acad Sci, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China.
关键词：
Binding energy;Bromine compounds;Cell membranes;Cytology;Hydrophobicity;Micelles;Molecular dynamics;Nuclear magnetic resonance;Raman spectroscopy;Sodium;Sodium sulfate;Spectroscopic analysis;Angiotensin II;Bi-layer;Binding free energy;Binding process;Concentration ratio;Conformational change;Cross peaks;Delivery process;Dipalmitoyl phosphatidylcholine;Head groups;Hexadecyl trimethyl ammonium bromide;Hydrophobic interactions;Lipid chains;MD simulation;Molecular dynamics simulations;NMR techniques;Nuclear magnetic resonance techniques;Phosphocholine;Polysorbate 20;Raman spectroscopic study;Receptor antagonists;Relaxation rates;Self-diffusion coefficients;Sodium dodecyl sulfate;Strong interaction;Tween-20;Valsartan;Soaps (detergents)
摘要：
Valsartan (VST) is one of the Angiotensin II receptor antagonists, which is widely used in clinical hypertension treatment. It is believed that VST incorporates into biological membranes before it binds to AT<inf>1</inf> receptor. Herein the interactions between VST and detergents, mimicking the membrane environment, were investigated by using nuclear magnetic resonance (NMR) techniques and molecular dynamics (MD) simulation. We observed that VST has two conformers (trans and cis) exchanging slowly in DPC (dodecyl- phosphocholine) micelles, a widely used detergent. The changes of chemical shifts, relaxation rates, and self-diffusion coefficients of VST protons indicate that both conformers have strong interactions with DPC. NOE cross peaks and MD simulation reveal that DPC interacts with VST not only through the hydrophobic lipid chain, but also the hydrophilic headgroup, locating VST at the charged headgroup and upper part of the micelles. Our results are in good agreement with the Raman spectroscopic studies of VST in the DPPC (dipalmitoyl-phosphatidylcholine) bilayers by Potamitis et al. (Biochim. Biophys. Acta.2011). The concentration ratio of trans over cis conformers is 0.94, showing that two conformers have the same affinities with the detergent, which is significantly smaller than our previous results obtained in SDS (sodium dodecyl sulfate) micelles. MD simulation suggested that the cis conformer has slightly lower binding free energy than the trans conformer when interacting with DPC. The conformational change of VST was further investigated in two detergents, CTAB (hexadecyltrimethylammonium bromide) and Tween-20 (polysorbate 20). Ratios of conformer A and B in the presence of detergents are in the order of DPC, CTAB <Tween-20 <SDS, which is correlated with the charge characters of their head groups. NMR investigations and MD simulations indicate that the electrostatic interaction plays an essential role in the binding process of VST with detergents, and the hydrophobic interaction influences the packing of the drug in the micelles. These results may be of help in understanding delivery processes of sartan drugs in cell membranes. ©2012 American Chemical Society.<br/>
语种：
英文
展开
Hydrogen-Bond-Mediated Asymmetric Cascade Reaction of Stable Sulfur Ylides with Nitroolefins: Scope, Application and Mechanism
作者：
Lu, Liang-Qiu;Li, Fang;An, Jing;Cheng, Ying;Chen, Jia-Rong;...
期刊：
CHEMISTRY-A EUROPEAN JOURNAL ,2012年18(13):4073-4079 ISSN：0947-6539
通讯作者：
Xiao, Wen-Jing
作者机构：
[Lu, Liang-Qiu; Cheng, Ying; Li, Fang; Xiao, Wen-Jing; Chen, Jia-Rong; An, Jing] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Hubei, Peoples R China.;[Xiao, Wen-Jing] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, 152 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Xiao, Wen-Jing] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, 152 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China.
关键词：
annulation;asymmetric synthesis;hydrogen bonds;rearrangement;sulfur;ylides
摘要：
A hydrogen-bond-mediated asymmetric [4+1] annulation/rearrangement cascade of stable sulfur ylides and nitroolefins was developed. This reaction provides a facile route to enantioenriched 4,5-substituted oxazolidinones in moderate to excellent isolated yields (65-96 %) with excellent stereocontrol (up to more than 95:5 d.r. and 97:3 e.r.). This methodology was successfully applied to the concise synthesis of two bioactive molecules. The stereocontrolled modes and mechanism have been proposed to explain the origin of this stereochemistry. Controlled cascade: A hydrogen-bond-mediated asymmetric [4+1] annulation/rearrangement cascade of stable sulfur ylides and nitroolefins was developed (see scheme). This reaction provides a facile route to enantioenriched 4,5-substituted oxazolidinones. Stereocontrolled modes and a mechanism are proposed to explain the origin of the stereochemistry. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
语种：
英文
展开
Hydrogen-Deuterium Exchange Reaction of 2-Benzylthio-5-Methyl-1,2,4-Triazolo [1,5-a] Pyrimidine Under Basic Conditions
作者：
Li, Fang;Chen, Qiong;Liu, Chen-Chen;Wu, Yue-Hong;Liu, Xiao-Peng;...
期刊：
Applied Magnetic Resonance ,2012年42(2):169-177 ISSN：0937-9347
通讯作者：
Yang, Guang-Fu
作者机构：
[Yang, Guang-Fu; Li, Fang; Liu, Xiao-Peng; Chen, Qiong; Liu, Chen-Chen; Wu, Yue-Hong] Cent China Normal Univ, Key Lab Pesticides & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
通讯机构：
[Yang, Guang-Fu] C;Cent China Normal Univ, Key Lab Pesticides & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
关键词：
Nuclear Magnetic Resonance;Exchange Rate;Nuclear Magnetic Resonance Spectrum;CD3OD;Nuclear Magnetic Resonance Spectroscopy
摘要：
Hydrogen-deuterium (H-D) exchange usually happens between D 2O/CD 3OD solvent and N-heterocyclic compounds in the presence of a base. 2-Benzylthio-5-methyl-1,2,4-triazolo[1,5-a]pyrimidine (BMTP) is an important intermediate for the synthesis of triazolopyrimidines with bioactivities. No exchange was observed when BMTP was dissolved in a CD 3OD solvent. However, a significant H-D exchange was observed after a small amount of NaOD had been added to the solution. According to the time-varying proton intensity, the rates (k ex) of H-D exchange were achieved under various temperatures and different amounts of NaOD. The results showed that the H-D exchange rates of H3, H4, and H5 were different under identical conditions, but were enhanced with increasing temperature and concentration of NaOD. A mechanism of H-D exchange that might be instructive in the ring-open reaction of 1,2,4-triazolo[1,5-a]pyrimidine derivatives was proposed. © 2011 Springer-Verlag.
语种：
英文
展开
Synthesis, Characterization, and Electrochemical Properties of Diruthenium Complexes Bridged by Anthraquinones
作者：
Li, Fei;Cheng, Jie;Chai, Xiaohong;Jin, Shan* ;Wu, Xianghua;...
期刊：
Organometallics ,2011年30(7):1830-1837 ISSN：0276-7333
通讯作者：
Jin, Shan
作者机构：
[Cheng, Jie; Wu, Xianghua; Liu, Sheng Hua; Jin, Shan; Chai, Xiaohong; Yu, Guang-Ao; Li, Fei] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.;[Chen, George Z.] Univ Nottingham, Sch Chem Environm & Min Engn, Nottingham NG7 2RD, England.
通讯机构：
[Jin, Shan] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
关键词：
[carbonyl;Bimetallic complexes;Binuclear ruthenium complexes;Diruthenium complex;Electronwithdrawing;Elemental analysis;Metal centers;UV/vis spectrophotometry;Aromatic compounds;Electrochemical properties;Electrochemistry;Ketones;Ruthenium;X ray crystallography;Ruthenium compounds
摘要：
We have prepared four isomeric binuclear ruthenium complexes, in which two ruthenium units have been attached to the 1,4- (4a), 1,5- (4b), 1,8- (4c), or 2,6-positions (4d) of a central anthraquinone (Aq) moiety, leading to packed (4c) or extended (4a,b,d) topologies. All of these bimetallic complexes were fully characterized by elemental analysis, <sup>1</sup>H, <sup>13</sup>C{ <sup>1</sup>H}, and <sup>31</sup>P NMR{<sup>1</sup>H} spectrometry, and UV/vis spectrophotometry. Moreover, the structures of 4a,b were established by X-ray crystallography. The electrochemical properties of the stable binuclear ruthenium complexes 4a-d were investigated, revealing that the two metal centers in 4a-c could interact with each other through an anthraquinone bridge, suggesting that the electron-withdrawing carbonyl chain actually functions as an effective bridge. ©2011 American Chemical Society.
语种：
英文
展开
A Highly Enantioselective Access to Chiral 1-(beta-Arylalkyl)-1H-1,2,4-triazole Derivatives as Potential Agricultural Bactericides
作者：
Lu, Wen-Chang;Cao, Xiu-Fang;Hu, Ming;Li, Fei;Yu, Guang-Ao;...
期刊：
Chemistry & Biodiversity ,2011年8(8):1497-1511 ISSN：1612-1872
通讯作者：
Liu, Sheng-Hua
作者机构：
[Yu, Guang-Ao; Li, Fei; Hu, Ming; Liu, Sheng-Hua; Lu, Wen-Chang] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Lu, Wen-Chang] Huazhong Agr Univ, Period Agcy, Wuhan 430070, Peoples R China.;[Cao, Xiu-Fang] Huazhong Agr Univ, Coll Sci, Wuhan 430070, Peoples R China.
通讯机构：
[Liu, Sheng-Hua] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
1,2,4-Triazoles;Antifungal activities;Enantioselective syntheses
摘要：
<jats:title>Abstract</jats:title><jats:p>A series of chiral 1‐(<jats:italic>β</jats:italic>‐arylalkyl)‐1<jats:italic>H</jats:italic>‐1,2,4‐triazole derivatives has been designed as potential antifungal agents. The target triazoles have been synthesized by using a chiral auxiliary as a controlling reagent. All of the compounds were obtained with high ee values, reaching up to 99%. Preliminary bioassay results have revealed that most of the synthesized compounds display significantly higher fungicidal activities against the species <jats:italic>Fusarium oxysporium, Rhizoctonia solani, Botrytis cinereapers, Gibberella zeae, Dothiorella gregaria</jats:italic>, and <jats:italic>Colletotrichum gossypii</jats:italic> than the commercial agent <jats:italic>triadimefon.</jats:italic> Moreover, some of the enantiomers have been found to display significant differences in activity.</jats:p>
语种：
英文
展开
Brønsted acid catalyzed Diels–Alder reactions of 2-vinylindoles and 3-nitrocoumarins: an expedient synthesis of coumarin-fused tetrahydrocarbazoles
作者：
Tan, Fen;Li, Fang;Zhang, Xiao-Xiao;Wang, Xu-Fan;Cheng, Hong-Gang;...
期刊：
Tetrahedron ,2011年67(2):446-451 ISSN：0040-4020
通讯作者：
Chen, Jia-Rong
作者机构：
[Wang, Xu-Fan; Cheng, Hong-Gang; Li, Fang; Tan, Fen; Xiao, Wen-Jing; Chen, Jia-Rong; Zhang, Xiao-Xiao] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.;[Chen, Jia-Rong] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, 152 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Chen, Jia-Rong] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, 152 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China.
关键词：
Organocatalysis;Diels-Alder reaction;Coumarin;Tetrahydrocarbazole
摘要：
A Brønsted acid catalyzed Diels–Alder reaction of 2-vinylindoles and 3-nitrocoumarins has been described. The methodology allows a rapid and expedient synthesis of a variety of coumarin-fused polycyclic indoles in good yields (up to 82%) with high diastereoselectivities (up to >19:1).
语种：
英文
展开
NMR study on iridium(III) complexes for identifying disulfonate substituted bathophenanthroline regio-isomers
作者：
Liu, Chenchen;Yu, Linpo;Liu, Yang;Li, Fang;Zhou, Ming*
期刊：
Magnetic Resonance in Chemistry ,2011年49(12):816-823 ISSN：0749-1581
通讯作者：
Zhou, Ming
作者机构：
[Yu, Linpo; Liu, Chenchen; Liu, Yang; Zhou, Ming] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Ctr Vitro Diagnost Technol, Suzhou 215123, Jiangsu, Peoples R China.;[Li, Fang; Liu, Chenchen] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.;[Zhou, Ming] SunaTech Inc, BioBay, Suzhou 215123, Jiangsu, Peoples R China.;[Zhou, Ming] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Ctr Vitro Diagnost Technol, 398 Ruoshui Rd,Suzhou Ind Pk, Suzhou 215123, Jiangsu, Peoples R China.
通讯机构：
[Zhou, Ming] C;Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Ctr Vitro Diagnost Technol, 398 Ruoshui Rd,Suzhou Ind Pk, Suzhou 215123, Jiangsu, Peoples R China.
关键词：
NMR;1H NMR;13C NMR;gHSQC;gHMBC;gCOSY;NOESY;bathophenanthroline;iridium(III) complexes
摘要：
<jats:p>A series of novel biscyclometalated iridium (III) complexes with an ancillary disulfonated bathophenanthroline (DSBP<jats:sup>2‐</jats:sup>) ligand, Ir(<jats:italic>L</jats:italic>)<jats:sub>2</jats:sub>DSBPNa, <jats:italic>L</jats:italic> = 2‐phenylpyridine (ppy), 2,4‐difluorophenylpyridine (fppy), and 1‐phenylisoquinoline (piq) were found to have two isomeric forms. The chemical structures of the isomers were determined by the one‐ and two‐dimensional <jats:sup>1</jats:sup>H and <jats:sup>13</jats:sup> C NMR studies. The isomeric state was proved to have originated from the disulfonate‐related regio‐isomer of the DSBP<jats:sup>2‐</jats:sup> ligand. Copyright © 2011 John Wiley & Sons, Ltd.</jats:p>
语种：
英文
展开
碱催化下***并嘧啶化合物氢氘交换的NMR研究
作者：
刘晨琛;陈琼;刘小鹏;李芳
作者机构：
[刘晨琛; 陈琼; 刘小鹏; 李芳] 华中师范大学化学学院,湖北 武汉 430079
会议名称：
第十六届全国波谱学学术会议
会议时间：
2010-11-23
会议地点：
海口
会议主办单位：
中国物理学会波谱学专业委员会
会议论文集名称：
第十六届全国波谱学学术会议论文集
摘要：
<正>***并嘧啶类杂环衍生物由于其分子结构中同时含有***和嘧啶这两类重要的活性结构单元,因而表现出了广谱的生物活性,在医药、农药以及材料等研究领域受到了人们的普遍关注。研究表明1,2,4-***并[1,5-a]嘧啶类化合物在碱催化下能够发生
语种：
中文
展开
