会议论文集名称：
2010 4th International Conference on Bioinformatics and Biomedical Engineering. [v.1.1]
关键词：
BBP;MDA;DPC;Carbonyl;Hepatic cells
摘要：
In order to study the effect of butyl benzyl phthalatee (BBP) on hepatic cells of rat, hepatic cells of rat were exposed to different dose (5 μmol / L, 20 μmol / L, 80 μmol / L) of BBP in vitro for 1 hour. Thiobarbituric acid (TBA) method , KCl-SDS precipitation method and Dinitrophenylhydrazone (DNPH) colorimetric method were used to detect malondialdehyde (MDA) content, DPC coefficient changes and content, respectively. MDA levels in hepatic cells showed a dose-dependent enhancement after BBP exposure. Significant MDA content was observed in all groups compared to control group. The content of carbonyl was all increased in exposure groups, especially in the group of 5μmol / L. DPC coefficient presented a slight increase compared with the control group but no significant difference in the dose of 5 μmol / L and 20 μmol / L. When the concentration of BBP increased to 80 μmol / L, DPC coefficient increased, and there was a significant difference compared with the control group. The carbonyl content was significantly increased in the dose of 5μmol / L. These results suggest that BBP can cause liver damage in rats, and might have toxic effects on the human body.
摘要：
目的探讨携带黑素瘤分化抗原MART-1基因的减毒单核细胞增多性李斯特菌(LM)对恶性黑素瘤的抑制作用及其机制.方法构建pERL3-MART-l载体,通过电转化建立携带MART-1基因的 Ainl B LM-MART-1和△act A/Ainl B LM-MART-1重组李斯特菌.浓度梯度稀释法测定各减毒菌株半数致死量(LDso).C57BU6小鼠随机分为磷酸盐缓冲液(PBS)组,△inl B LM-MART-1组和△act A/Ainl B LM-MART-1组.首次免疫后第7天于小鼠腹部皮下接种B16Fl0细胞,第14天、21天重复免疫小鼠,观察并记录肿瘤大小及小鼠生存状态.应用实时定量PCR检测肿瘤组织中MART-1表达量.流式细胞术检测小鼠脾细胞中CD4~+CD25~+T细胞阳性率.结果经鉴定成功构建△inl B LM-MART-1和△act A/Ainl B LM-MART-1.减毒株△inl B LM和△act A/△inl B LM的LDso比LM-ECDe标准株毒力分别下降100倍和 10 000倍.体内抑瘤试验显示,与PBS组比较,△inl B LM-MART-1组抑瘤率为46.95%,△act A/Ainl B LM-MART-1抑瘤率为83.96%,差异均有统计学意义(P<0.05和0.01).PBS组、△inl B LM-MART-1组和 △act A/△inl B LM-MART-1组肿瘤组织中MART-1表达量递增,以PBS组表达量为1,后两组为8.988± 0.207和11.315±0.445,各组间差异均有统计学意义(P值均<0.05),且三组小鼠脾细胞中CD4+CD25+T细胞阳性率依次为(2.52±0.20)%、(1.14±0.13)%和(0.44±0.15)%,组间差异均有统计学意义(P值< 0.01).结论携带MART-1基因的减毒LM毒性明显低于LM标准株,而且可以有效抑制黑素瘤生长,延长小鼠生存期
期刊：
2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010),2010年:1-4 ISSN：2151-7614
通讯作者：
Luo, Qin
作者机构：
[Hu, Jing; Wang, Xingyun; Luo, Qin; Chu, Peili; Zhou, Qingchun] Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Luo, Qin] H;Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)
会议时间：
2010-06-18
会议地点：
成都
会议主办单位：
[Chu, Peili;Wang, Xingyun;Hu, Jing;Luo, Qin;Zhou, Qingchun] Huazhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.
会议论文集名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)论文集
关键词：
plinton;Nematostella vectensis
摘要：
With genome-wide screening of N. vectensis, two new groups of Polinton were identified, beside five Polinton groups deposited in Repbase. The typical N. vectensis Polinton is in length of 15-20kb and has long TIRs with a 6-bp TSDs. it encodes as many as 6 different proteins, including retroviral-like integrase (INT), adenovirus protease (PRO), DNA polymerase B (POLB) and putative ATPase (ATP). These ancient N. vectensis Polinton groups fall to two clades by phylogenetic analysis. One clade evolves evolves to vertebrates together with large scale gene linkage of N. vectensis, another clade following to the evolution of fly and nematode genome.
摘要：
The ability of Listeria monocytogenes resisting bile stress is very important for its successful infection and colonization in the human gastrointestinal tract. Some studies have indicated that the alternative sigma factor Sigma B (σB), encoded by sigB, is at least partly involved in bacteria resisting many adverse conditions. In order to investigate the role of L. monocytogenes Sigma B in bile tolerance in the gastrointestinal tract, sigB mutant strain EGDΔsigB was constructed, and in parallel with wild type strain EGD, exposed to 3% bile salt&#65288;sublethal concentration for bacteria&#65289;with or without pre-exposure to acid (pH 4.5), alkali (pH 9.0), or osmotic (0.3M NaCl) conditions by mimicking the human gastrointestinal juice for 30 min. Their growth and survival rates were examined after 5 hours cultivation. The results were shown (&#8560;) wild type strain EGD was more resistant to 3% bile salt stress than sigB mutant strain EGDΔsigB; (&#8561;) Both alkali- and osmotic-adapted EGD and EGDΔsigB were more resistant to 3% bile salt than nonadapted strains; (iii) acid-adapted EGD and EGDΔsigB didn't increase their ability to resist 3% bile salt. These data indicate that L. monocytogenes σB is required for bile tolerance in the gastrointestinal tract, and also reveal some σB-dependent cross-protection mechanisms involving in tolerance of alkaline (or osmolality) and bile salt stress.
