期刊：
Bulletin of Environmental Contamination and Toxicology,2007年78(1):61-65 ISSN：0007-4861
通讯作者：
Wang, X.-D.
作者机构：
[Wang, X.-D.] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, X.-D.] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Biosorption;Algal Cell;Anhydrous Sodium Sulfate;Relative Standard Deviation;Organophosphorus Pesticide
期刊：
JOURNAL OF SEPARATION SCIENCE,2007年30(15):2506-2512 ISSN：1615-9306
通讯作者：
Wang, Xuedong
作者机构：
[Wang, Xuedong] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, Xuedong] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
作者：
Juneau, Philippe;Qiu, Baosheng（邱保胜）;Deblois, Charles P.
期刊：
TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY,2007年89(4):609-625 ISSN：0277-2248
通讯作者：
Juneau, P.(juneau.philippe@uqam.ca)
作者机构：
[Juneau, Philippe; Deblois, Charles P.] Department of Biological Sciences–Environmental Toxicology Research Center (TOXEN), Canada Research Chair on Ecotoxicology of Aquatic Microorganisms, University of Québec in Montréal, C.P. 8888, Succursale Centre-Ville, Montreal, Quebec, Canada H3C 3P8;[Qiu, Baosheng] College of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, P. R. China
通讯机构：
[Philippe Juneau] D;Department of Biological Sciences–Environmental Toxicology Research Center (TOXEN), Canada Research Chair on Ecotoxicology of Aquatic Microorganisms, University of Québec in Montréal, C.P. 8888, Succursale Centre-Ville, Montreal, Quebec, Canada H3C 3P8
摘要：
Chlorophyll fluorescence-based methods have been used for many years to study the effect of environmental factors (including herbicides) on plants, and, with the development of very sensitive fluorometers, these methods have become very useful in ecotoxicological studies. This review provides a summary of the research done over the last 10 years concerning the study, by using chlorophyll fluorescence, of the herbicide toxic effects. Although numerous parameters are available, it is striking to notice that the majority of the studies done in the recent years used only a limited number of parameters, thus restraining in some way the interpretation of the obtained results. Furthermore, the use of the maximal photosystem II quantum yield to evaluate toxic effect of herbicides is probably not the best parameter due to its relatively low sensitivity. One should take the advantage of using other parameters such as the operational PSII quantum yield and the non-photochemical quenching since they integrate the entire physiological state of the plant and therefore should be more sensitive. We also recommend the systematic determination of EC50 values in future studies since such determination will permit a more accurate comparison between published reports.
期刊：
Bulletin of Environmental Contamination and Toxicology,2007年78(5):368-372 ISSN：0007-4861
通讯作者：
Wang, X.-D.
作者机构：
[Wang, X.-D.] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Col Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, X.-D.] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
作者机构：
[Zheng, Yonglian] Huazhong Normal Univ, Coll Life Sci & Technol, State Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China.;Huazhong Normal Univ, Coll Life Sci & Technol, Natl Ctr Crop Mol Breeding, Wuhan 430070, Peoples R China.
通讯机构：
[Zheng, Yonglian] H;Huazhong Normal Univ, Coll Life Sci & Technol, State Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China.
关键词：
cytoplasmic male sterility;mitochondria;mRNA decay;stem‐loop;polyadenylation
摘要：
The co-transcribed orf355-orf77 region of the mitochondrial genome is associated with S cytoplasmic male sterility (CMS-S) in maize; the amounts of its 1.6- and 2.8-kb transcripts were previously shown to be greatly reduced in fertility-restored microspores relative to the amounts in sterile plants. To investigate the mechanism underlying this reduction, detailed analysis of the 5' and 3' termini of these transcripts was conducted. Using 3' RACE analysis, the polyadenylation sites of the 1.6- and 2.8-kb transcripts were mapped adjacent to a 3' stem-loop, which may play an important role in stabilizing their 3' ends. No difference was found between the polyadenylation sites in sterile and fertility-restored microspores that could account for the differences in orf355-orf77 transcript levels. The 5' terminus of the 1.6-kb transcript was further studied by primer extension; the result revealed that there was a deletion of nine nucleotides only in fertility-restored microspores, and that this deletion eliminated a 5' stem-loop sequence. We propose that the elimination of the 5' stem-loop in the fertility-restored microspores could be the cause of the degradation of the 1.6-kb transcript. Because the 2.8-kb transcript can be cleaved to generate the 1.6-kb transcript, the amount of the 2.8-kb transcript is also reduced in fertility-restored microspores.
作者：
Li Ping;Liu De-li*;Nahimana Liberat;Chen Shu-li;Yang Xi;...
期刊：
环境科学学报(英文版),2006年18(3):525-529 ISSN：1001-0742
通讯作者：
Liu De-li
作者机构：
[Li Ping; Chen Shu-li; Liu De-li; Yang Xi; Zhao Li; Liu, DL; Nahimana Liberat] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Liu De-li] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
关键词：
high nitrogen removal;aerobic denitrification;wastewater treatment;identification
摘要：
Three new bacteria HS-03, HS-043 and HS-047 isolated from different ecosystems were found capable of aerobic denitrification. The potential application of these strains in wastewater treatment under aerobic conditions was investigated. These three bacteria all presented high nitrogen removal from wastewater that more than 98% of 10 mmol/L nitrate could be removed in 12-24 h by adding cheap external carbon source and low concentration of iron as well as molybdate. The mechanism at molecular level was analyzed. The success of this aerobic denitrification applied to wastewater treatment may serve as an alternative to enhance the practical nitrogen removal from wastewater. Main biochemical and physiological features of these strains were characterized. The 16S rDNA sequences were compared with the published data in GenBank by using BLAST. The results of phenotype and genotype proved that strain HS-03 and HS-047 belonged to Pseudomonas stutzeri and Pseudomonas pseudoalcaligenes respectively. Strain HS-043 was identified as Delftia acidovorans of which denitrifying activity has not previously been explored.
作者机构：
[程琴; 吴刚; 杨娇艳; 张勇; 梅娟] College of Life Science, Huazhong Normal University, Wuhan 430079, China;[宋冬林; 张勇] Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China;[王红梅] School of Environmental Studies, China University of Geosciences, Wuhan 430074, China
通讯机构：
College of Life Science, Huazhong Normal University, China
作者机构：
[Liu, DL] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hebei, Peoples R China.;Huazhong Univ, Minist Educ China, Key Lab Pesticide & Chem Biol, Wuhan, Peoples R China.;Univ Tokyo, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan.
通讯机构：
[Liu, DL] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hebei, Peoples R China.
摘要：
The difference of sensitivity to indole-3-acetic acid (IAA) combined with horseradish peroxidase (HRP) in K562 and BXPC-3 cells was investigated. The cell proliferation was determined by MTT assay. The cell cycle and apoptosis of K562 and BXPC-3 cells were examined by a fluorescence flow cytometer (FCM) and terminal deoxynucleotidyl transferase-mediated dUTP nickrend labeling (TUNEL) respectively. The experimental results show that IAA and HRP could inhibit BXPC-3 cell proliferation greatly compared with K562 cell during the first 48 h. The cell cycle was arrested predominantly at G2/M phase in K562 and BXPC-3 cells. The cell apoptosis of K562 and BXPC-3 was induced by IAA/HRP. There was a significant difference between the two cell lines since BXPC-3 cells were more sensitive than K562 cells by treatments with combination of IAA and HRP.
作者机构：
[Wei, Lei; Yu, Jin-Hong; Ding, Yi] Key Laboratory of MOE for Plant Developmental Biology/College of Life Sciences, Wuhan University, Wuhan, Hubei, China;College of Life Sciences, Hunan University of Science and Technology, Xiangtan, Hunan, China;College of Life Sciences, Huazhong Normal University, Wuhan, Hubei, China;[Hu, Yao-Jun] Key Laboratory of MOE for Plant Developmental Biology/College of Life Sciences, Wuhan University, Wuhan, Hubei, China<&wdkj&>College of Life Sciences, Hunan University of Science and Technology, Xiangtan, Hunan, China;[Liu, Shu-Nan] Key Laboratory of MOE for Plant Developmental Biology/College of Life Sciences, Wuhan University, Wuhan, Hubei, China<&wdkj&>College of Life Sciences, Huazhong Normal University, Wuhan, Hubei, China
通讯机构：
[Ding Yi] K;Key Laboratory of MOE for Plant Developmental Biology/College of Life Sciences, Wuhan University, Wuhan, Hubei, China
关键词：
Hybrid rice;Male-sterile;Protein;Two-dimensional polyacrylamide gel electrophoresis
摘要：
For searching out male sterility-related proteins (polypeptides) in rice (Oryza sativa L.), we examined the difference of panicle protein (polypeptides) between hybrid rice (Wujin2A/R168, Wujin5A/R988) and their parents (male-sterile line Wujin2A, Wujin5A, and restorer line R168, R988) at the formation stage of pollen mother cell by two-dimensional electrophoresis (2-DE). The results revealed that the 2-DE polypeptide maps were similar among these experimental materials. A small group of polypeptides were disappeared in 2-DE polypeptide maps of male-sterile line (Wujin2A, Wujin5A) by comparing to restorer line (R168, R988) and the first filial (F<sub>1</sub>) generation (Wujin2A/R168, Wujin5A/R988). The isoelectric points of these polypeptides were pI 5.8-6.5, molecular weight 42.7×10<sup>3</sup> - 66.2×10<sup>3</sup>.
