作者机构：
[储昭升; 尹延震; 贾永见; 叶碧碧; 吴爱平] State Environmental Protection Key Laboratory for Lake Pollution Control, Research Center of Lake Eco-environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;[吴爱平] Institute of Ecology, College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha 410128, China;[贾永见; 程凯] College of Life Science, Central China Normal University, Wuhan 430000, China;[储昭升; 尹延震; 贾永见; 叶碧碧; 吴爱平] State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
通讯机构：
State Environmental Protection Key Laboratory for Lake Pollution Control, Research Center of Lake Eco-environment, Chinese Research Academy of Environmental Sciences, China
摘要：
Cotton aphid (Aphis gossypii Glover) is one of the most important economic pests in the world. Long-term unreasonable usage of insecticides has made cotton aphid developing insecticide-resistance, which frequently leads to serious occurrences of cotton aphid in many regions. It is regarded effective and environmentally friendly to control aphids through utilizing plant resistance. In this study, a translationally controlled tumor protein gene, GhTCTP1, was isolated in cotton. It belongs to TCTP subfamily and encodes a protein of 168 amino acids. GhTCTP1 expression was suppressed in cotton plants under cotton aphid attack, but its expression level was up-regulated in the wounded cotton leaves. The choice test and no-choice test demonstrated that overexpression of GhTCTP1 in Arabidopsis enhanced plant resistance to green peach aphid (Myzus persicae). Quantitative RT-PCR analysis revealed that the defense response genes related to salicylic acid signaling pathway were activated in the GhTCTP1 overexpressing transgenic plants. Content of total amino acids was decreased, and phenylalanine ammonialyase activity was altered in leaves of the transgenic Arabidopsis plants, compared with those in wild type. Furthermore, the callose amount in transgenic Arabidopsis leaves was more than that of wild type. These data suggested that GhTCTP1 might be involved in regulation of plant tolerance to aphids, and can be potentially applied in improving aphid-resistance of crops by genetic manipulation.
摘要：
Plastics such as polyvinyl chlorides (PVC) are widely used in many indoor constructed environments; however, their unbound chemicals, such as di-(2-ethylhexyl) phthalates (DEHP), can leach into the surrounding environment. This study focused on DEHP's effect on the central nervous system by determining the precise DEHP content in mice brain tissue after exposure to the chemical, to evaluate the specific exposure range. Primary neuronal-astrocyte co-culture systems were used as in vitro models for chemical hazard identification of DEHP. Oxidative stress was hypothesized as a probable mechanism involved, and therefore the total reactive oxygen species (ROS) concentration was determined as a biomarker of oxidative stress. In addition, NeuriteTracer, a neurite tracing plugin with ImageJ, was used to develop an assay for neurotoxicity to provide quantitative measurements of neurological parameters, such as neuronal number, neuron count and neurite length, all of which could indicate neurotoxic effects. The results showed that with 1 nmol/L DEHP exposure, there was a significant increase in ROS concentrations, indicating that the neuronal-astrocyte cultures were injured due to exposure to DEHP. In response, astrocyte proliferation (gliosis) was initiated, serving as a mechanism to maintain a homeostatic environment for neurons and protect neurons from toxic chemicals. There is a need to assess the cumulative effects of DEHP in animals to evaluate the possible uptake and effects on the human neuronal system from exposure to DEHP in the indoor environment.
作者机构：
[张忠杰; 焦铭; 廖文莉; 马萍; 武阳; 陈姣娥] College of Basic Medical, Hubei University of Science and Technology, Xianning , China;[杨旭; 马萍; 武阳] Laboratory of Environment Biomedicine, School of Life Science, Central China Normal University, Wuhan , China
通讯机构：
College of Basic Medical, Hubei University of Science and Technology, Xianning, China
作者机构：
[Zhang, Weiying; Liu, Deli] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Du, Dan] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Zhang, Weiying; Tang, Yong; Timchalk, Charles; Du, Dan; Smith, Jordan; Lin, Yuehe] Pacific NW Natl Lab, Richland, WA 99352 USA.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Au nanoparticles;Biomarker;Immunochromatographic biosensor;Organophosphorus pesticides;Trichloropyridinol
摘要：
A portable immunochromatographic strip-based biosensor for direct detection of trichloropyridinol (TCP), a specific biomarker of exposure to chlorpyrifos, in human saliva sample is presented. In this approach, a series of immunoreactions was performed on the test strip, where the targeted analytes (TCP) bound to the Au nanoparticles-labeled antibodies on the conjugate pad to form analyte-Au-antibody conjugates, and then free Au-labeled antibodies were captured by TCP-BSA in the test zone. Captured Au nanoparticles, increased with decreased levels of analytes, can be observed visibly without any equipment and later quantified by a calorimetric reader. Several experimental parameters were optimized including Au nanoparticle-to-TCP antibody coupling ratio, the amount of Au-labeled TCP antibody, immunoreaction time, the pretreatment of sample pad and the preparation of stock solution of Au-TCP antibody that realize sensitivity, selectivity and direct detection of TCP. Under optimal conditions, this biosensor displays a highly linear range of 0.625-20 ng/mL TCP, with a detection limit of 0.47 ng/mL. Moreover, the immunosensor was successfully used for direct analysis of human saliva sample without any pretreatment. These results demonstrate that this Au nanoparticles-based immunochromatographic test strip (ITS) provides a simple, accurate, and quantitative tool for TCP detection and holds a great promise for point-of-care and in-field analysis of other biomarkers. (C) 2013 Elsevier B.V. All rights reserved.
作者机构：
[刘旭东; 熊棣; 李慧; 杨旭; 柯玉洁; 问华肖; 张玉超; 丁书茂; 秦晓丹; 李睿] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, Wuhan 430079, China
通讯机构：
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, China
作者：
Jie, Rui;Zhou, Quan;Wang, Jin Song;Liang, Yun He;Liao, Ting Ting;...
期刊：
Applied Mechanics and Materials,2013年380-384:4353-4358 ISSN：1660-9336
通讯作者：
Geng, H.(genghui@mail.ccnu.edu.cn)
作者机构：
[Yu, Jin Hui; Liu, De Li; Liao, Ting Ting; Zhou, Quan; Wang, Jin Song; Geng, Hui; Jie, Rui; Liang, Yun He] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan 430079, China
通讯机构：
[Geng, H.] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, , Wuhan 430079, China
会议名称：
2013 International Conference on Vehicle and Mechanical Engineering and Information Technology, VMEIT 2013
摘要：
Schima superba is a common dominant tree species in evergreen broad-leaved forest in subtropical China. Despite its multiple usages in wood industry, reforestation and traditional Chinese medicine, its genetic diversity is poorly studied. To help studying its genetic diversity and structure in the future, after microsatellite enrichment and screening, we identified 16 microsatellites in S. superba. These markers showed polymorphism in three populations. The number of alleles per locus ranged from 3 to 32 with a mean of 14. Within populations, the observed and unbiased expected heterozygosities ranged from 0.048 to 0.926 and from 0.048 to 0.949, respectively. The newly developed 16 microsatellites will be useful for investigating the genetic diversity and structure from large scale patterns to fine-scale structures in this species.
摘要：
In this study, interaction effects of high light (HL) and zinc on two Microcystis aeruginosa strains (toxic CPCC299 and non-toxic CPCC632) were investigated. Our results suggested that CPCC632 was more sensitive to high zinc (HZn; 7.8 × 10−6 M) concentration than CPCC299 when acclimated to HL (500 μmol photons m−2 s−1). When M. aeruginosa strains were treated 20 min to HL (2000 μmol photons m−2 s−1), the maximal and the operational PSII quantum yields (ΦM and ) of the two strains decreased, but CPCC632 (50 μmol photons m−2 s−1 (low light (LL)) + HZn) has the biggest decrease among the four growth conditions. Under LL growth conditions, CPCC632 was more sensitive to HL stress than CPCC299. However, these differences disappeared when they were grown under HL condition. These results suggested that CPCC632 had the lowest ability to acclimate HL stress under LL + HZn conditions. We also showed that the response mechanisms to HL stress of M. aeruginosa were linked to the change of pigments, energy dissipation processes, and microcystin content (for the toxic strain). Our data indicated that different effects of HL and zinc on M. aeruginosa strains may influence the dominance of different strains in aquatic system.
作者：
Yu, Jin Hui;Qi, Ting;Xiong, Li;Li, Qian;Wang, Jin Long;...
期刊：
Applied Mechanics and Materials,2013年380-384:4170-4174 ISSN：1660-9336
通讯作者：
Liu, D. L.(deliliu2002@yahoo.com.cn)
作者机构：
[Yuan, Yong Ze; Yu, Jin Hui; Li, Qian; Wang, Jin Long; Liu, De Li; Qi, Ting; Xiong, Li; Geng, Hui] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan 430079, China
通讯机构：
[Liu, D. L.] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, , Wuhan 430079, China
摘要：
Blue mold, caused by Penicillium. italicum, is one of the most damaging postharvest diseases of citrus fruit. P. italicum Sterol 14α-demethylase (PiCYP51), an important enzyme in membrance sterol biosynthesis, is a key target of antifungal compounds for citrus disease caused by P. italicum. The three-dimensional structure of PiCYP51 from P. italicum Chinese isolate (HS-1) was constructed through homology modeling basing on the crystal structure of human CYP51. After molecular dynamics (MD) simulation, the refined model was assessed by PROCHECK on the quality. Following evaluation on the reliability was performed by investigating the binding interaction of two commercial sterol 14α-demethylase inhibitors (DMIs) with the enzyme. The binding mode predicted by the molecular docking revealed that the DMIs interacted with PiCYP51 mainly through hydrogen-bonding and hydrophobic interactions. Furthermore, the results were compatible with the detected EC50 values, which were determined as 0.25 and 0.31mg/L for tebuconazole and diniconazole. The binding mode of antifungal agents with PiCYP51 can provide references for DMIs optimization, virtual screening, or de novo antifungal compounds design.
作者机构：
[张忠杰; 焦铭; Shan, Shi-Gang; 陈姣娥; 马萍] College of Basic Medical, Hubei University of Science and Technology, Xianning 437100, China;[杨旭; 武阳; 马萍] Laboratory of Environment Science, College of Life Science, Huazhong Normal University, Wuhan 430079, China
通讯机构：
Laboratory of Environment Science, College of Life Science, Huazhong Normal University, China
作者机构：
[叶染枫; 刘旭东; 李慧; 杨旭; 闵安娜; 张玉超; 陈明清; 刘锋明; 赵莉琴] Laboratory of Environmental Science, College of Life Sciences, Huazhong Normal University, Wuhan 430079, China
通讯机构：
Laboratory of Environmental Science, College of Life Sciences, Huazhong Normal University, China
