作者：
Philip H.-S.Jen;LUAN Rui-Hong;WU Fei-Jian;SUN Xin-De
期刊：
生理学报,2007年59(6):805-813 ISSN：0371-0874
通讯作者：
Luan, R.H.
作者机构：
[LUAN Rui-Hong; SUN Xin-De] School of Life Sciences, East China Normal University;[WU Fei-Jian] School of Life Sciences, Central China Normal University;[Philip H.-S.Jen] Division of Biological Sciences, University of Missouri-Columbia
摘要：
The echolocating big brown bats (Eptesicus fuscus) emit trains of frequency-modulated (FM) biosonar signals with duration, amplitude, repetition rate, and sweep structure changing systematically during interception of their prey. In the present study, the sound stimuli of temporally patterned pulse trains at three different pulse repetition rates (PRRs) were used to mimic the sounds received during search, approach, and terminal stages of echolocation. Electrophysiological method was adopted in recordings from the inferior colliculus (IC) of midbrain. By means of iontophoretic application of bicuculline, the effect of GABAergic inhibition on the intensity sensitivity of IC neurons responding to three different PRRs of 10, 30 and 90 pulses per second (pps) was examined. The rate-intensity functions (RIFs) were acquired. The dynamic range (DR) of RIFs was considered as a criterion of intensity sensitivity. Comparing the average DR of RIFs at different PRRs, we found that the intensity sensitivity of some neurons improved, but that of other neurons decayed when repetition rate of stimulus trains increased from 10 to 30 and 90 pps. During application of bicuculline, the number of impulses responding to the different pulse trains increased under all stimulating conditions, while the DR differences of RIFs at different PRRs were abolished. The results indicate that GABAergic inhibition was involved in modulating the intensity sensitivity of IC neurons responding to pulse trains at different PRRs. Before and during bicuculline application, the percentage of changes in responses was maximal in lower stimulus intensity near to the minimum threshold (MT), and decreased gradually with the increment of stimulus intensity. This observation suggests that GABAergic inhibition contributes more effectively to the intensity sensitivity of the IC neurons responding to pulse trains at lower sound level.
期刊：
Bulletin of Environmental Contamination and Toxicology,2007年78(5):368-372 ISSN：0007-4861
通讯作者：
Wang, X.-D.
作者机构：
[Wang, X.-D.] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Col Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, X.-D.] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
期刊：
Pest Management Science,2007年63(3):296-300 ISSN：1526-498X
通讯作者：
Peng, Jianxin
作者机构：
[Peng, Jianxin] Cent China Normal Univ, Inst Entomol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Peng, Jianxin] C;Cent China Normal Univ, Inst Entomol, Wuhan 430079, Peoples R China.
关键词：
Spodoptera exigua;Syngrapha falcifera multiple nucleopolyhedrovirus;peritrophic membrane;environmental scanning electron microscope;Calcofluor white M2R
作者机构：
[Zheng, Yonglian] Huazhong Normal Univ, Coll Life Sci & Technol, State Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China.;Huazhong Normal Univ, Coll Life Sci & Technol, Natl Ctr Crop Mol Breeding, Wuhan 430070, Peoples R China.
通讯机构：
[Zheng, Yonglian] H;Huazhong Normal Univ, Coll Life Sci & Technol, State Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China.
关键词：
cytoplasmic male sterility;mitochondria;mRNA decay;stem‐loop;polyadenylation
摘要：
The co-transcribed orf355-orf77 region of the mitochondrial genome is associated with S cytoplasmic male sterility (CMS-S) in maize; the amounts of its 1.6- and 2.8-kb transcripts were previously shown to be greatly reduced in fertility-restored microspores relative to the amounts in sterile plants. To investigate the mechanism underlying this reduction, detailed analysis of the 5' and 3' termini of these transcripts was conducted. Using 3' RACE analysis, the polyadenylation sites of the 1.6- and 2.8-kb transcripts were mapped adjacent to a 3' stem-loop, which may play an important role in stabilizing their 3' ends. No difference was found between the polyadenylation sites in sterile and fertility-restored microspores that could account for the differences in orf355-orf77 transcript levels. The 5' terminus of the 1.6-kb transcript was further studied by primer extension; the result revealed that there was a deletion of nine nucleotides only in fertility-restored microspores, and that this deletion eliminated a 5' stem-loop sequence. We propose that the elimination of the 5' stem-loop in the fertility-restored microspores could be the cause of the degradation of the 1.6-kb transcript. Because the 2.8-kb transcript can be cleaved to generate the 1.6-kb transcript, the amount of the 2.8-kb transcript is also reduced in fertility-restored microspores.
摘要：
Formaldehyde (FA) is a genotoxic and mutagenic substance. In 2004, IARC (International Agency for Research on Cancer) concluded that FA is carcinogenic in humans after reevaluating the available evidence on the carcinogenicity of FA. Although many studies have shown that FA had extensive genotoxicity including DNA-protein crosslinks (DPC) and DNA single strand breaks (DSSB), most of these studies only discussed the effects of FA at high levels. In this study, KCl-SDS assay and single cell gel electrophoresis (SCGE) were used to investigate the formation and repair process of FA-induced DPC and DSSB in human peripheral blood lymphocytes and Hela cell lines. KCl-SDS assay was applied to detect DPC induced by liquid FA in human peripheral blood lymphocytes in vitro. The results showed that FA could induce DPC at high levels (≥50 micro M). By combining the results of KCl-SDS assay and SCGE, it could be determined that FA would induce DNA-DNA crosslinks (DDC) when FA concentration was more than 25 microM. The repair process of FA-induced DPC was studied with KCl-SDS assay in Hela cell lines and the results demonstrated that FA-induced DPC could be significantly repaired after 18 hours. The SCGE was also used to determine FA-induced DSSB and its repair process in Hela cell lines. The results demonstrated that DNA breakages, which is capable of being induced by FA at a low level (<30 microM), enabled to be repaired completely in 90 minutes.
作者机构：
[Meng-Rong CHENG; Sen-Lin JI] College of Life Science, Central China Normal University, Wuhan 430079, China;Department of Medicine, the University of Hong Kong, Hong Kong, China;[Qing-Yang HUANG] College of Life Science, Central China Normal University, Wuhan 430079, China<&wdkj&>Department of Medicine, the University of Hong Kong, Hong Kong, China
关键词：
type 2 diabetes;genetics;association studies;candidate genes;genome-wide scans
摘要：
Type 2 diabetes mellitus （T2DM） is a complex disease characterized by hyperglycemia, insulin resistance, and impaired insulin secretion. T2DM is under strong genetic control. Identification and characterization of genes involved in determining T2DM will contribute to a greater understanding of the pathogenesis of T2DM, and ultimately might lead to the development of better diagnosis, prevention and treatment strategies. Efforts to identify T2DM susceptibility genes have focused on candidate gene approach （association studies） and genome-wide scans （linkage analyses）. In this article, we review the current status for mapping and identification of genes for T2DM, with a focus on some promising regions （or genes） and future prospects.
作者：
Li Ping;Liu De-li*;Nahimana Liberat;Chen Shu-li;Yang Xi;...
期刊：
环境科学学报(英文版),2006年18(3):525-529 ISSN：1001-0742
通讯作者：
Liu De-li
作者机构：
[Li Ping; Chen Shu-li; Liu De-li; Yang Xi; Zhao Li; Liu, DL; Nahimana Liberat] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Liu De-li] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
关键词：
high nitrogen removal;aerobic denitrification;wastewater treatment;identification
摘要：
Three new bacteria HS-03, HS-043 and HS-047 isolated from different ecosystems were found capable of aerobic denitrification. The potential application of these strains in wastewater treatment under aerobic conditions was investigated. These three bacteria all presented high nitrogen removal from wastewater that more than 98% of 10 mmol/L nitrate could be removed in 12-24 h by adding cheap external carbon source and low concentration of iron as well as molybdate. The mechanism at molecular level was analyzed. The success of this aerobic denitrification applied to wastewater treatment may serve as an alternative to enhance the practical nitrogen removal from wastewater. Main biochemical and physiological features of these strains were characterized. The 16S rDNA sequences were compared with the published data in GenBank by using BLAST. The results of phenotype and genotype proved that strain HS-03 and HS-047 belonged to Pseudomonas stutzeri and Pseudomonas pseudoalcaligenes respectively. Strain HS-043 was identified as Delftia acidovorans of which denitrifying activity has not previously been explored.
作者：
Wang, Xu;Douglas, Steven D.;Peng, Jin-Song;Zhou, Dun-Jin;Wan, Qi;...
期刊：
AMERICAN JOURNAL OF PATHOLOGY,2006年169(5):1663-1670 ISSN：0002-9440
通讯作者：
Ho, Wen-Zhe
作者机构：
Univ Penn, Childrens Hosp Philadelphia, Sch Med,Joseph Stokes Jr Res Inst,Dept Pediat, Div Allergy & Immunol,Abramson Res Ctr, Philadelphia, PA 19104 USA.;Wuhan Ctr Dis Prevent & Control, Virol Lab, Wuhan, Peoples R China.;Wuhan Univ, Renmin Hosp, Dept Pediat, Wuhan 430072, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan, Peoples R China.;[Ho, Wen-Zhe] Univ Penn, Childrens Hosp Philadelphia, Sch Med,Joseph Stokes Jr Res Inst,Dept Pediat, Div Allergy & Immunol,Abramson Res Ctr, Room 1202B,34th St & Civ Ctr Blvd, Philadelphia, PA 19104 USA.
通讯机构：
[Ho, Wen-Zhe] U;Univ Penn, Childrens Hosp Philadelphia, Sch Med,Joseph Stokes Jr Res Inst,Dept Pediat, Div Allergy & Immunol,Abramson Res Ctr, Room 1202B,34th St & Civ Ctr Blvd, Philadelphia, PA 19104 USA.
作者机构：
[Liu, DL] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hebei, Peoples R China.;Huazhong Univ, Minist Educ China, Key Lab Pesticide & Chem Biol, Wuhan, Peoples R China.;Univ Tokyo, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan.
通讯机构：
[Liu, DL] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hebei, Peoples R China.
