关键词：
small interfering RNA;adhesion molecule CD146;vascular endothelial cell
摘要：
Our previous study has demonstrated that CD146 molecule is a biomarker on vascular endothelium, which is involved in angiogenesis and tumor growth. However the mechanism behind is not clear. Here we have for the first time developed a novel CD146 blockade system using CD146 siRNA to study its function on endothelial cells. Our data showed that CD146 siRNA specifically blocked the expression of CD146 on both mRNA and protein levels, leading to the significant suppression of HUVEC proliferation, adhesion and migration. These results demonstrate that CD146 plays a key role in vascular endothelial cell activity and angiogenesis, and CD146 siRNA can be used as a new inhibitor for anti-angiogenesis therapy.
作者机构：
[骆启桂; 王国秀; 吴少斌] College of Life Science, Huazhong Normal University, Wuhan 430079, China;Institute of Insect Resource, Huazhong Agricultural University, Wuhan 430070, China;[隗权; 邓长盛; 黄大钱] Houhe National Nature Reserve, Wufeng 440000, China;[Zha Y.] College of Life Science, Huazhong Normal University, Wuhan 430079, China, Institute of Insect Resource, Huazhong Agricultural University, Wuhan 430070, China
通讯机构：
[Zha, Y.] C;College of Life Science, , Wuhan 430079, China
期刊：
Molecular Genetics and Metabolism,2006年88(4):295-306 ISSN：1096-7192
通讯作者：
Huang, QY
作者机构：
[Huang, Qing-Yang] Department of Medicine, The University of Hong Kong, Hong Kong, PR China<&wdkj&>College of Life Sciences, Central China Normal University, Wuhan, Hubei 430079, PR China;[Kung, Annie Wai Chee] Department of Medicine, The University of Hong Kong, Hong Kong, PR China
通讯机构：
[Huang, QY ] ;Univ Hong Kong, Dept Med, Hong Kong, Hong Kong, Peoples R China.
关键词：
genetics;bone mineral density;osteoporosis;linkage;association
摘要：
Osteoporosis is a common disease with a strong genetic component. In recent years, some progress has been made in understanding the genetic basis of osteoporosis. Genetic factors contribute to osteoporosis by influencing not only bone mineral density but also bone size, bone quality, and bone turnover. Meta-analysis has been used to define the role of several candidate genes in osteoporosis. Some quantitative trait loci that regulate bone mass identified by linkage studies in humans and experimental animals have been replicated in multiple populations. Genes that cause monogenic bone diseases also contribute to regulation of bone mass in the normal population. Genome-wide association studies and functional genomics approaches have recently begun to apply to genetic studies of osteoporosis. In the future, not only single gene but also the entire gene networks involved in osteoporosis and regulation of bone mass will systematically be discovered through integrative genomics. (c) 2006 Elsevier Inc. All rights reserved.
期刊：
Journal of Bone and Mineral Metabolism,2006年24(2):132-137 ISSN：0914-8779
通讯作者：
Deng, HW
作者机构：
Creighton Univ, Osteoporosis Res Ctr, Omaha, NE 68131 USA.;Cent China Normal Univ, Coll Life Sci, Hubei, Peoples R China.;Hunan Normal Univ, Coll Life Sci, Hunan, Peoples R China.;Xian Jiaotong Univ, Key Lab Biomed Informat Engn, Minist Educ, Xian 710049, Peoples R China.;Xian Jiaotong Univ, Inst Mol Genet, Sch Life Sci & Technol, Xian 710049, Peoples R China.
通讯机构：
[Deng, HW] C;Creighton Univ, Osteoporosis Res Ctr, 601 N 30th St,Suite 6787, Omaha, NE 68131 USA.
摘要：
Osteoporosis has a strong genetic component, but the genes involved are poorly defined. Genome-wide scans in multiple populations have identified chromosome 1p36 as one region linked to bone mineral density (BMD). The tumor necrosis factor receptor 2 (TNFR2) at 1p36 is a positional and functional candidate gene in osteoporosis. In this study, we conducted linkage and association tests between the CA repeat polymorphism of the TNFR2 gene and BMD in two large independent samples using the quantitative transmission disequilibrium test (QTDT) program. The first group of subjects was composed of 1836 individuals from 79 multigeneration pedigrees. The second group was a randomly ascertained set of 636 individuals from 157 nuclear families. We found no evidence of association or linkage for spine or hip BMD in the samples of the multigenerational pedigrees or nuclear families. Through testing for association and for linkage, our data do not support the TNFR2 gene as a QTL underlying hip or spine BMD variation in our Caucasian populations.
摘要：
Although there has been a growing body of literature showing the neural correlation of forward masking caused by a pure tone masker in the auditory neurons, relative few studies have addressed the description of how the forward masking caused by a noise burst, especially a sequence of noise burst, is transformed into neuronal representation in the central auditory system. Using a noise forward masking paradigm under free field stimuli conditions, this in vivo study was devoted to exploring it in the inferior collicular (IC) neurons of the mouse (Mus musculus KM). A total of 96 IC neurons were recorded. Rate-intensity functions (RIFs) with and without the presentation of masker, sustained noise burst (SNB) or segmental noise burst (SGNB), were measured in 51 neurons. We found that the relative masker intensities were distributed over a wide range between 21 dB below the minimum threshold (MT) and 19 dB above the MT of the corresponding probe tone. The masking effect of the SGNB on firing rate in nearly half of neurons (type I, 45.10%) was stronger than that of the SNB (P<0.001), whereas in a smaller fraction of neurons (type III, 17.65%), it was weaker than that of the SNB (P<0.001). There was no significant difference in masking effect between the SNB and SGNB in type II neurons (37.25%, P>0.05). Irrespective of type I or type III neurons, the inhibitory effects of both kinds of maskers were all greater at lower probe intensities but decreased significantly with the increase of probe intensity (P<0.001). Interestingly, as the probe intensity increased, the difference of masking effect between the SNB and SGNB disappeared (P>0.05). In addition, we observed that temporal masking pattern could be transformed when the masker was changed from the SNB to SGNB. The main type of this transformation was from early-inhibition to proportional-inhibition pattern (53.85%, 7/13). Our data provide the evidence that the inhibitory effects of these two maskers have differential weights over time and intensity domains of the IC neurons responding to a pure tone. This suggests that the forward masking of noise is by no means the source of simply suppression in neuronal firing rate. There might be a few of active neural modulating ways in which the coding of temporal acoustical information can be operated.
作者机构：
[Chen, XW] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China.;Cent China Normal Univ, Inst Entomol, Wuhan 430070, Peoples R China.;Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China.
通讯机构：
[Chen, XW] C;Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China.
关键词：
HaSNPV;p78/83;Ha2;WASP;baculovirus;structural protein
摘要：
The open reading frame 2 (ha2) of the Helicoverpa armigera single nucleocaspid nucleopolyhedrovirus (HaSNPV), a conserved gene in 111ost baculoviruses from lepidopteran insects such as p78/83 of the Autographa californica MNPV, was characterized. It is 1242 bp long and potentially encodes a 45.9 kDa. Ha2 is conserved among baculoviruses from lepicdopteran insects. Ha2 transcripts were detected front 16 to 96 11 post infection (hpi) of HzAM1 cells. Rabbit polyclonal antiserum against a GST-HA2 fusion protein reacted with three protein of 50, 46 and 35 kDa at 24-72 hpi of HzAM1 cells. Anti OpMNPV ORF2 (homologue of HA2) antibody reacted only with the 46 and 35 kDa proteins in HaSNPV-infected cells. These results demonstrate that Ha2 is modified at the mRNA or protein levels. Western blot analysis showed that only the 50kDa product of HA2 is a structural component of proteins of both the budded virus (BV) and occlusion-derived virus (ODV) phenotypes. HA2-EGFP fusion protein showed that HA2 is localized primarily in the nucleus of HzAM1 infected cells. The HA2 was found to co-localize with actin by labelling of actin with Rhordamine-Phalloidin. In summary, the data indicated that HA2 is a structural protein and interacts with host cell actin. (c) 2005 Elsevier B.V. All rights reserved.
摘要：
A simple and sensitive electrochemical immunosensor with impedance labelless detection and novel data processing method was investigated. One-step copolymerization was used to electrochemically deposit an antibody impregnated polypyrrole film on a glassy carbon electrode surface for the immunosensor. Impedance measurements provided a labelless or reporterless method to detect antibody (Ab)-antigen (Ag) interactions. Dimensionless analysis was employed to successfully process the measured impedance data. Since the method derived unit impedance change to eliminate or reduce the variation of the bulk electronic properties of Ab/polypyrrole films, the signal to noise ratio (S/N) was significantly improved for high sensitivity and specificity. Nonspecific binding effect was studied by array electrode chips and was found out that the polypyrrole electrode without antibody attachment had much stronger nonspecific binding effect than the Ab/polypyrrole electrode; incubation followed by thoroughly washing significantly reduced the nonspecific interference. 10 pg/ml detection limit and superior specificity were achieved by the method, demonstrating a highly sensitive labelless immunosensor in comparison with the detection limit of ng -microgram/ml for the reported polypyrrole based immunosensors. The electrochemical immunosensors presented in this paper, due to its simplicity, low cost, high sensitivity and superior specificity, could be an invaluable tool for clinical diagnostics and could have potential applications in drug discovery, environmental and food analysis.
摘要：
The prevalence of asthma keeps on increasing worldwide, especially in western societies over last 40 years. The mechanism of asthma is unclear. Recently, concern about indoor air pollution as a risk factor for asthma has been arisen. In present study, 25 Kun Ming male mice were placed in an air chamber containing respective formaldehyde ( FA) concentration of 0, 0.5, 1.0, 3.0 mg/m(3), and 3.0 mg/m3 with Capsazepine ( CPZ, a specific antagonist of vanilloid receptor)-pretreatment in five testing groups ( n= 5 per group) for inhale experiments. The inhaled groups were exposed to gaseous FA for 6 hours each day in 10 successive days. After exposure, the concentrations of IL4 in blood serum and broncho alveolar lavage fluid (BALF) were measured. Experimental results showed that the IL4 level in serum was too low to be detected; and the concentrations of IL4 in BALF increased in a dose-dependent manner. However, for the CPZ-pretreated group the IL4 level in BALF decreased significantly ( compared with 3.0 mg/m(3) FA inhaled group, p < 0.01). This paper describes experimental animal methods to probe IL4 level, an important indicator for IgE response. The studies in this paper indicated that gaseous FA might induce acquired atopy by type II VR1 signaling system. These findings suggested that indoor air pollutants such as FA might be key risk factors for the rise in asthma cases, and type II VR1 signaling system might be one of the mechanisms for the rise.
作者：
Philip H.S.Jen;LUAN Rui-Hong;WU Fei-Jian;SUN Xin-de
期刊：
生理学报,2005年57(2):225-232 ISSN：0371-0874
通讯作者：
Luan, R.H.
作者机构：
[LUAN Rui-Hong; SUN Xin-de] College of Life Sciences,East China Normal University;[WU Fei-Jian] College of Life Sciences,Central China Normal University;[Philip H.S.Jen] Division of Biological Sciences,University of Missouri-Columbia
摘要：
Temporal features of sound convey information vital for behaviors as diverse as speech recognition by human and echolocation by bats. However, auditory stimuli presented in temporal proximity might interfere with each other. Although much progress has been made in the description of this phenomenon from psychophysical studies, the neural mechanism responsible for its formation at central auditory structures especially at the inferior colliculus (IC), a midbrain auditory nucleus which practically receives massive bilateral projections from all the major auditory structures in the brainstem, remains unclear. This study was designed to investigate it in vivo by using electrophysiological recording from the inferior collicular neurons of the big brown bat, Eptesicus fuscus. In our results, the responses of 12 (38%, n= 31) neurons to the test sound (leading sound) were obviously inhibited by the masker (lagging sound). The inhibitory effects in these neurons were correlated with the inter-stimulus level difference (SLD) and the inter-stimulus onset asynchrony (SOA) interval. The strength of backward masking increased with the masker intensity increasing, the test sound intensity decreasing and the SOA interval shortening. There were no obvious effects of backward masking on the responses of many other neurons (52%, 16/31), and yet in a part of these neurons, the neural inhibition of responses to the test sound was observed at the special SLD and the special SOA intervals. Moreover, few of the 31 sampled IC neurons (10%, 3/31) displayed facilitating responses to the test sound at the special SLD and the special SOA intervals. These data demonstrate that a lot of IC neurons are involved in the generation of the backward masking of acoustical perception. It is conjectured that the temporal dynamic integration between the leading inhibitory inputs evoked by the masker sound and the excitatory inputs evoked by the test sound might play a key role in shaping the acoustical response characteristics of the IC neurons.
作者：
Wang Dan;Pi Jianhui;Tan Jia;Tang Jia;Wu Feijian;...
期刊：
生理学报,2005年57(1):59-65 ISSN：0371-0874
通讯作者：
Wang, D.
作者机构：
[Wang Dan; Tang Jia; Tan Jia; Wu Feijian; Chen Qicai] School of Life Sciences, Central China Normal University;[Pi Jianhui] Department of Biology, Huaihua College of Hunan
摘要：
In order to explore the possible mechanisms by which ethologically relevant sounds can be extracted from complex auditory environments, this study examined the effects of weak noise on the rate-intensity functions (RIFs) of neurons responding to tone burst in the inferior colliculus (IC) of nine mice (Mus musculus Km). Under free field stimuli conditions, a total of 112 IC neurons were recorded. RIFs with and without simultaneous presentation of weak noise, of which the intensity was relative to 5 dB below minimum threshold of tone burst, were measured in 44 IC neurons. By means of evaluating the changes of dynamic range (DR), slope of RIFs, and percent inhibition at different tone burst intensities evoked by the weak noise, three types of variations in RIFs were observed, i. e.,inhibition (39/44, 88.6%), facilitation (2/44, 4.6%), and no effectiveness (3/44, 6.8%). Statistical analysis indicated that only inhibitory effect of weak noise was significant (P ＜ 0.001, n = 39). The inhibitory effect of weak noise was greater at lower stimulus intensity of tone burst but decreased significantly with increased stimulus intensity (P ＜ 0.0001, n = 39). In addition, the DR and slope of RIFs became narrower and steeper with weak noise presentation, respectively (P ＜ 0.01, n = 31). The results from the present study suggest that weak noise exerts a dynamic modulatory action on acoustical intensity sensitivity of IC neurons, which possibly leads to a better understanding of neural mechanisms underlying the extraction of sound signals from natural auditory scenes.
