作者机构：
[Wang, Hanzhong] Chinese Acad Sci, State Key Lab Virol, WIV, Wuhan, Peoples R China.;Cent China Normal Univ, Wuhan, Hubei, Peoples R China.;Xian Ning Ctr Dis Control & Prevent, Xian Ning, Hubei, Peoples R China.;Chinese Acad Sci, Huazhong Normal Univ, Coll Life Sci, Wuhan, Peoples R China.
通讯机构：
[Wang, Hanzhong] C;Chinese Acad Sci, State Key Lab Virol, WIV, Wuhan, Peoples R China.
摘要：
Of 322 stool specimens collected from children with diarrhea from October 2005 through September 2006 in Wuhan, China, group A rotavirus was identified in 101 (31.4%). The most prevalent group A rotavirus genotype was G3P[8] (62.6%), followed by G1P[8](17.6%), G1+G3P[8](8.8%), G3P[4](6.6%), G1P[4](2.2%), and G9P[8](2.2%). The G9 strains were first detected in Wuhan.
作者机构：
[Haiyan Shi; Xiulan Wang; Dengdi Li; Wenkai Tang; Hong Wang; Wenliang Xu; Xuebao Li] College of Life Sciences,HuaZhong Normal University
通讯机构：
College of Life Sciences, HuaZhong Normal University, China
关键词：
cotton;14-3-3 protein;gene expression
摘要：
The 14-3-3 protein, highly conserved in all eukaryotic cells, is an important regulatory protein. It plays an important role in the growth, amplification, apoptosis, signal transduction, and other crucial life activities of cells. A cDNA encoding a putative 14-3-3 protein was isolated from cotton fiber cDNA library. The cDNA, designated as Gh14-3-3L (Gossypium hirsutum 14-3-3-like),is 1,029 bp in length (including a 762 bp long open reading frame and 5'-/3'-untranslated regions) and deduced a protein with 253 amino acids. The Gh14-3-3L shares higher homology with the known plant 14-3-3 proteins, and possesses the basic structure of 14-3-3 proteins: one dimeric domain, one phosphoralated-serine rich motif, four CC domains, and one EF Hand motif. Northern blotting analysis showed that Gh14-3-3L was predominantly expressed during early fiber development, and reached to the peak of expression in 10 days post anthers (DPA) fiber cells, suggesting that the gene may be involved in regulating fiber elongation. The gene is also expressed at higher level in both ovule and petal, but displays lower or undetectable level of activity in other tissues of cotton.
期刊：
JOURNAL OF EXPERIMENTAL BOTANY,2007年58(12):3227-3238 ISSN：0022-0957
通讯作者：
Li, Xue-Bao
作者机构：
[Huang, Geng-Qing; Li, Li; Li, Xue-Bao; Wang, Xiu-Lan] Huazhong Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Li, Xue-Bao] H;Huazhong Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
关键词：
Cotton;"expression profiles of GhTUA genes";"fibre elongation";fibre-specific;microtubule;"overexpression in yeast cells"
摘要：
The microtubule cytoskeleton may play an important role in the polarized growth of fibre cells that are single-cell trichomes on the surface of cotton ovules. To investigate whether the high expression levels of α-tubulin genes are correlated with fibre elongation, nine GhTUA genes (cDNAs) encoding α-tubulins with 449–451 amino acid residues were isolated and characterized in cotton. The GhTUA genes share high sequence homology at the nucleotide level (62–93% identity) in the coding region and at the amino acid level (89–99% identity), and can be classified into two subgroups. Real-time quantitative RT-PCR analysis revealed that seven out of the nine GhTUA genes are predominantly expressed in developing fibres. Among them, GhTUA9 displays the highest level of expression, revealing its fibre specificity. The GhTUA9 transcripts in fibres reached its peak value between 5–10 DPA, and dramatically declined to undetectable levels as the ovule matured further, suggesting that its expression is developmentally-regulated in fibres. The GhTUA9 gene including the promoter region was isolated from the cotton genome. To demonstrate the specificity of the GhTUA9 promoter, the 5′-flanking region, including the promoter and 5′-untranslated region, was fused with the GUS gene. Histochemical assays demonstrated that the GhTUA9:GUS gene was specifically expressed in elongating fibres. Overexpression of GhTUA9 in fission yeast (Schizosaccharomyces pombe) promoted atypical longitudinal growth of the host cells by 1.4–1.7-fold, indicating that the GhTUA9 gene is involved in cell elongation. Given all the above results, it is proposed that the GhTUA9 gene may play an important role in fibre elongation.
作者机构：
[Jen, Philip H. -S.] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA.;Univ Missouri, Interdisciplinary Neurosci Program, Columbia, MO 65211 USA.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Jen, Philip H. -S.] U;Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA.
作者机构：
[Chengwei Zhang; Linlin Guo; Xiulan Wang; Hui Zhang; Haiyan Shi; Wenliang Xu; Xuebao Li] College of Life Science, Central China Normal University
通讯机构：
College of Life Science, Central China Normal University, China
关键词：
cotton ADF gene;actin-depolymerizing factor;sequence analysis;molecular evolution;gene different expression
摘要：
Actin depolymerizing factor (ADF), highly conserved in all eukaryotic cells, is a low molecular mass of actin-binding protein, which plays a key role in modulating the polymerizing and depolymerizing of the actin filaments. Four cDNAs (designated GhADF2, GhADF3, GhADF4, and GhADF5, respectively) encoding ADF proteins were isolated from cotton (Gossypium hirsutum) fiber cDNA library. GhADF2 cDNA is 705 bp in length and deduces a protein with 139 amino acids. GhADF3 cDNA is 819 bp in length and encodes a protein of 139 amino acids. GhADF4 cDNA is 804 bp in length and deduces a protein with 143 amino acids. GhADF5 cDNA is 644 bp in length and encodes a protein of 141 amino acids. The molecular evolutionary relationship of these genes was analyzed by means of bioinformatics. GhADF2 is closely related to GhADF3 (99% identity) and PetADF2 (89% iden- tity). GhADF4 is closely related to AtADF6 (78% identity), and GhADF5 is closely related to AtADF5 (83% identity). These results demonstrated that the plant ADF genes are highly conserved in structure. RT-PCR analysis showed that GhADF2 is predominantly expressed in fiber, whereas, GhADF5 is mainly expressed in cotyledons. On the other hand, it seems that GhADF3 and GhADF4 have no tissue specificity. Expression levels of different ADF genes may vary considerably in the same cell type, suggesting that they might be involved in regulating tissue development of cotton and the each ADF isoform may diverge to form the functional difference from the other ADFs during evolution.
摘要：
Crystal structures of the L3MBTL1 MBT repeats in complex with histone H4 peptides dimethylated on Lys20 (H4K20me2) show that only the second of the three MBT repeats can bind mono- and dimethylated histone peptides. Its binding pocket has similarities to that of 53BP1 and is able to recognize the degree of histone lysine methylation. An unexpected mode of peptide-mediated dimerization suggests a possible mechanism for chromatin compaction by L3MBTL1.
期刊：
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,2007年26(2):62-65 ISSN：1554-0014
通讯作者：
Wang, Fengyang
作者机构：
Hainan Univ, Coll Life Sci & Agr, Haikou 570228, Peoples R China.;Huazhong Normal Univ, Coll Life Sci, Wuhan, Peoples R China.;[Wang, Fengyang] Hainan Univ, Coll Life Sci & Agr, Renmin Rd 58, Haikou 570228, Peoples R China.
通讯机构：
[Wang, Fengyang] H;Hainan Univ, Coll Life Sci & Agr, Renmin Rd 58, Haikou 570228, Peoples R China.
摘要：
The small molecule meso-Tetra (α,α,α,α-o-phenylacetamide benzene) porphyrin was synthesized through the condensation of o-nitrobenzaldehyde and pyrrole followed by reduction of the meso-tetra (o-nitrophenyl) porphyrin. The small molecule, without carrier, was used as complete antigen to immunize BALB/ C mice. Spleen cells producing high titer antibody were removed and fused with myeloma cells of SP2/0 origin. Using a conventional immunization protocol, stable murine monoclonal antibodies (MAbs) producing cell lines to meso-Tetra (α,α,α,α-o-phenylacetamide benzene) porphyrin 1F2 were obtained. Subclass determination showed that the clones produce IgG2a types of MAbs. The analytical results of HPLC and MALDI/TOFMS suggest that the purity of MAb 1F2 is 100%, and MAb 1F2 has a relative molecular weight of 156678.8 Da. Our results demonstrated that small molecule meso-Tetra (α,α,α,α-o-phenylacetamide benzene) porphyrin, as semiantigen without carrier, can elicit the formation of MAbs.
期刊：
Bulletin of Environmental Contamination and Toxicology,2007年78(1):61-65 ISSN：0007-4861
通讯作者：
Wang, X.-D.
作者机构：
[Wang, X.-D.] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, X.-D.] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Biosorption;Algal Cell;Anhydrous Sodium Sulfate;Relative Standard Deviation;Organophosphorus Pesticide
作者机构：
[杨劭; 杨娇艳; 肖敏] College of Life Sciences, Central China Normal University, wuhan, 430079, China;[肖文精] College of Chemistry, Central China Normal University, wuhan, 430079, China
通讯机构：
[YANG, S.] C;College of Life Sciences, , wuhan, 430079, China
作者机构：
[罗勤; 周青春; 邓灵福; 高强; 刘德立] College of Life Science, Central China Normal University
关键词：
PrfA
摘要：
To study some essential elements of a PrfA-dependent promoter of Listeria monocytogenes, a series of promoter mutants incorporated into upstream of a promoterless lacZ gene were constructed from a known listerial PrfA-dependent promoter, inlC promoter, by PCR-mediated site-directed mutagenesis and recombinant PCR technique and then electroporated into L. monocytogenes wild-type strain P14, prfA * mutant Pl4a and prfA deletion mutant A42. The corresponding transcription activities of altered promoters were measured by the beta-galactosidase assay. The results showed that a PrfA-box-like sequence ("pseudo-PrfA-box"), TTAACAGCGTTTGTTAA, 22bp downstream of the transcriptional start site of PinlC had no ability to enhance or inhibit the PrfA-dependent transcription of inlC promoter, even it was modified to the "ideal PrfA-box" TTAACATTTGTTAA. However, there was almost no PrfA-dependent transcriptional activity from the mutants deletion of the inlC original PrfA-box. Moreover, altered spacing between 3'-end of the PrfA-box and 5'-end of the -10 box in the inlC promoter region affected transcription efficiency dramatically, which also happened in another promoter-dependent promoter, plcA promoter. Those above suggested that besides the "PrfA-box", additional unknown PrfA-dependent promoter structure(s) or sequence(s) might be required for the PrfA binding to the promoter and initiation of transcription. Furthermore, the distance between the PrfA-box and the -10 box should be fixed to 22 or 23bp for the PrfA-dependent transcription.
作者机构：
[Peng, Jianxin] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Inst Entomol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Peng, Jianxin] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
关键词：
AfMNPV;Caspase 3;Cyclosporin A;Cytochrome c;Spodoptera litura cell line (SL-ZSU-1, SL-1)
摘要：
We investigated the influence of cytochrome c on apoptosis induced by Anagrapha (Syngrapha) falcifera multiple nuclear polyhedrosis virus (AfMNPV). Microscopic observation revealed that infection of SL-1 cells with AfMNPV resulted in apoptosis, displaying apoptotic bodies in fluorescent-stained nuclei of AfMNPV-infected SL-1cells. Western blot analysis demonstrated that AfMNPV-induced apoptosis in insect SL-1 cells was significantly inhibited by cyclosporin A which blocked a translocation of cytochrome c from the mitochondria to the cytosol. As determined by using AC-DEVD-AFC as substrate, the activity of caspase-3 in AfMNPV-induced cells was detected as early as 4 h post infection, gradually increased with time extension, and reached a highest level after 16 h of infection. However, activity of caspase-3 in apoptotic cells decreased in the presence of cyclosporin A (30 mu M), indicating that activation of caspase-3 in SfaMNPV-induced cells was dependent on the release of cytochrome c from the mitochondria. In addition, cyclosporin A could markedly inhibit mitochondrial transmembrane potential (Delta Psi m) disruption in undergoing apoptotic cells. These data indicate that cytochrome c plays a key role in AfMNPV-induced apoptosis in S. litura cells and may be required for caspase activation during the induction of apoptosis. (C) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
期刊：
JOURNAL OF SEPARATION SCIENCE,2007年30(15):2506-2512 ISSN：1615-9306
通讯作者：
Wang, Xuedong
作者机构：
[Wang, Xuedong] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, Xuedong] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
作者：
Jia Tang;Fei-Jian Wu;Dan Wang;Jen, Philip H. -S.;Qi-Cai Chen*
期刊：
CHINESE JOURNAL OF PHYSIOLOGY,2007年50(4):187-198 ISSN：0304-4920
通讯作者：
Qi-Cai Chen
作者机构：
[Qi-Cai Chen] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA.;Univ Missouri, Interdisciplinary Neurosci Program, Columbia, MO 65211 USA.
通讯机构：
[Qi-Cai Chen] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
Natural auditory environment consists of multiple sound sources that are embedded in ambient strong and weak noise. For effective sound communication and signal analysis, animals must somehow extract biologically relevant signals from the inevitable interference of ambient noise. The present study examined how a weak noise may affect the amplitude sensitivity of neurons in the mouse central nucleus of the inferior colliculus (IC) which receives convergent excitatory and inhibitory inputs from both lower and higher auditory centers. Specifically, we studied the amplitude sensitivity of IC neurons using a probe (best frequency pulse) and a masker (weak noise) under simultaneous masking paradigm. For most IC neurons, weak noise masking increases the minimum threshold and decreases the number of impulses. Noise masking also increased the slope and decreased the dynamic range of the rate amplitude function of these IC neurons. The strength of this noise masking was greater at low than at high sound amplitudes. This variation in the amplitude sensitivity of IC neurons in the presence of the weak noise was mostly mediated through GABAergic inhibition. These data indicate that in the real world the ambient weak noise improves amplitude sensitivity of IC neurons through GABAergic inhibition while inevitably decreases the range of overall auditory sensitivity of IC neurons.
