关键词：
cDNA-AFLP;Cytoplasmic male sterility;Restorer of fertility;PPR;26S protease;Maize (Zea mays L.)
摘要：
In CMS-S of maize (Zea mays L.), mitochondrial orf355-orf77 and the nuclear restorer of fertility designated as Rf3/rf3 interact to control fertility of gametophytes. Genes expressed differentially in young leaves and pollen were screened using complementary DNA amplified fragment length polymorphism (cDNA-AFLP) combined with a set of Rf3/rf3 near isogenic lines. Sixty-four combinations of AFLP primers produced 2,450 observed transcript-derived fragments (TDFs). The differentially expressed TDFs were assorted into seven different expressed types, of which, 33 TDFs expressed only in fertile pollen and three expressed in fertile materials specifically were cloned and sequenced. Sequencing analysis indicated that 33 tentative unique genes were obtained and assigned into eight different categories including protein synthesis and fate, post-transcript regulation, signal transduction, and so on. TDF-E1 was indicated representing a gene coding a protein containing pentatricopeptide repeat (PPR) motif by BLASTx analysis, which temporarily denominated as PPRE1. Real-time polymerase chain reaction analysis revealed that PPRE1 expressed far more strongly in pollen of S-Mo17
Rf3Rf3
than in other materials investigated. Polymorphism analysis of PPRE1 in genomes showed difference between S-Mo17
Rf3Rf3
and S-Mo17
rf3rf3
. Taken together, we proposed that PPRE1 may be a promising candidate gene for restoration of fertility in maize S-CMS, as a set of near-isogenic lines had identical cytoplasm and eliminated most noise from nuclear genetic backgrounds. Another gene, which corresponds to TDF-A5 and codes 26S protease regulatory particle non-ATPase subunit5 was also discussed about its potential role in participating in the repression of mitochondrial programmed cell death associated with sterile pollen in S-type cytoplasm.
作者机构：
[Zhang, Aidong; Du, Dan; Tu, Haiyang; Ye, Xiuping; Zhang, Jiande; Zeng, Yan] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Liu, Deli] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
zirconia nanoparticles;solid-phase extraction;square-wave voltammetry;methyl parathion;NERVE AGENTS;MULTILAYER FILMS
摘要：
A sensitive electrochemical stripping voltammetric method for analyzing organophosphate (OP) compounds was developed based on solid-phase extraction (SPE) at zirconia (ZrO2) nanoparticles modified electrode. ZrO2 nanoparticles were proved as a new sorbent for SPE of OP pesticides. Because of the strong affinity of ZrO2 for the phosphoric group, nitroaromatic OPs can strongly bind to the ZrO2 nanoparticle surface. The combination of SPE with square-wave voltammetry (SWV) provided a fast, sensitive, and selective electrochemical method for nitroaromatic OP compounds using methyl parathion (MP) as a model. The stripping response was highly linear over the MP range of 0.003-2.0 mu g/mL, with a detection limit of 0.001 mu g/mL. The fast extraction ability of ZrO2 nanoparticles makes it promising sorbent for various solid-phase extractions. (C) 2008 Elsevier B.V. All rights reserved.
作者：
Wang, Xin;Luo, Feng;Wu, Fei-Jian;Chen, Qi-Cai;Jen, Philip H. S.*
期刊：
NEUROREPORT,2008年19(8):861-865 ISSN：0959-4965
通讯作者：
Jen, Philip H. S.
作者机构：
[Chen, Qi-Cai; Luo, Feng; Wu, Fei-Jian; Wang, Xin] Cent China Normal Univ, Coll Life Sci, Wuhan, Peoples R China.;[Jen, Philip H. S.] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA.;[Jen, Philip H. S.] Univ Missouri, Interdisciplinary Neurosci Program, Columbia, MO 65211 USA.
通讯机构：
[Jen, Philip H. S.] U;Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA.
关键词：
bat, duration selectivity, inferior colliculus, pulse–echo pairs, recovery cycle
作者机构：
[Wu, F. J.; Chen, Q. C.; Bibikov, N. G.] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Bibikov, N. G.] NN Andreyev Acoust Inst, Moscow 117036, Russia.
通讯机构：
[Wu, F. J.] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
We recorded extracellular activity from 402 single units located in the inferior colliculus (IC) of barbiturate-anesthetized albino mice. The stimuli were pure tones at characteristic frequency (CF) with durations of 10, 40 and 100 ms and intensities ranged from 5 to 25 dB above unit's minimum threshold (MT). The tones were presented with different repetition rates (RRs) ranging from 0.2 to 20.0 Hz. At low intensities (5 dB above MT, determined at RR of 0.5 Hz) the great majority of units exhibited a strong decline of their responses when the stimulus RR was increased. About one-half of the units did not respond to 40 ms tones when they were stimulated with the RR of 3.0 Hz. This effect was even more pronounced for 100 ms tones. Generally, the increase in stimulus intensity led to an increase in the high-frequency border of RR. Nevertheless, even at intensities of 20-30 dB above MT, some units showed no response when the RR exceeded 5.0 Hz. In many cases the band-pass or high-pass duration tuning of the single unit was transformed to low-pass or all-pass when the rate was low enough to guarantee the independence of successive presentations of the stimuli. Responses of a very small group of IC units, however, were enhanced when the RR was increased. Our data have shown that the changes in the RR radically modify many features of the neural response (number of spikes, latency, discharge pattern, duration selectivity). We suggest that long-lasting inhibitory processes may be induced by low intensity stimuli in many units of the IC. (C) 2008 Elsevier B.V. All rights reserved.
作者机构：
[Yang, Jiao-Yan] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Lu, Liang-Qiu; Ming, Zhi-Hui; An, Jing; Hua, Qiu-Lin] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticides & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Lu, Liang-Qiu] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticides & Chem Biol, Wuhan 430079, Peoples R China.
摘要：
The crystal structure of the title compound, C14H11NO4, is influenced by N - H⋯O and O - H⋯O hydrogen bonds, linking mol-ecules into one-dimensional tapes running along the [010] direction.
通讯机构：
[Li, Xue-Bao] H;Hua Zhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
Fasciclin-like arabinogalactan proteins (FLAs), a subclass of arabinogalactan proteins (AGPs), are usually involved in cell development in plants. To investigate the expression profiling as well as the role of FLA genes in fiber development, 19 GhFLA genes (cDNAs) were isolated from cotton (Gossypium hirsutum). Among them, 15 are predicted to be glycosylphosphatidylinositol anchored to the plasma membranes. The isolated cotton FLAs could be divided into four groups. Real-time quantitative reverse transcriptase polymerase chain reaction results indicated that the GhFLA genes are differentially expressed in cotton tissues. Three genes (GhFLA1/2/4) were specifically or predominantly expressed in 10 days post-anthesis fibers, and the transcripts of the other four genes (GhFLA6/14/15/18) were accumulated at relatively high levels in cotton fibers. Furthermore, expressions of the GhFLA genes are regulated in fiber development and in response to phytohormones and NaCl. The identification of cotton FLAs will facilitate the study of their roles in cotton fiber development and cell wall biogenesis.
摘要：
Tobacco hydroxyproline-rich glycopeptide systemin precursor A (TobpreproHypSys-A), from which TobHypSys I and II are released, plays a crucial role in defense responses. Here, we investigated the expression of TobpreproHypSys-A and the activity of defense proteins in tobacco organs during wounding. Expression was induced more rapidly in upper, non-wounded leaves than in lower, wounded leaves. At 24 h after mechanical wounding, expression was low in the roots, but increased in the stems and flowers, although to a lesser extent than in the leaves. At 3 or 10 d after insect-wounding, expression did not differ among organs, suggesting that TobpreproHypSys-A could be induced globally and continuously throughout such stress. During that period, the activity of two defense proteins -- PPO and PI -- was consistent with the expression of TobpreproHypSys-A in various organs. This indicates that those proteins also could be regulated by TobHypSys, both globally and continuously.
作者：
Du, X. Z.;Zhou, L.;Zhao, H. B.;Wang, Y. F.*;Gui, J. F.
期刊：
Fish Physiology and Biochemistry,2008年34(2):175-184 ISSN：0920-1742
通讯作者：
Wang, Y. F.
作者机构：
[Zhao, H. B.; Du, X. Z.; Wang, Y. F.; Zhou, L.] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Gui, J. F.] Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
通讯机构：
[Wang, Y. F.] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
Hir/Hira (histone regulation) genes were first identified in yeast as negative regulators of histone gene expression. It has been confirmed that HIRA is a conserved family of proteins present in various animals and plants. In this paper, the cDNAs of the Hira homolog named CagHira and CaHira were isolated from gynogenetic gibel carp (gyno-carp) and gonochoristic color crucian carp (gono-carp) respectively. The full-length CagHira is 3,860 bp in length with an open reading frame (ORF) of 3,033 bp that encodes 1,011 amino acids, while the full-length CaHira is 3,748 bp in length and also has an ORF of 3,033 bp. The deduced amino acid sequences of both Hira homologs contain seven WD domains and show high identity with other HIRA family members. RT-PCR analyses revealed strong expression of Hira in the ovaries, whereas no expression was detected in the testes of either of the fishes. Hira transcription was not detected in the liver of gyno-carp, but a high level of Hira mRNA was observed in gono-carp. The temporal expression pattern showed that the Hira mRNA is consistently expressed during all embryonic development stages in gyno-carp. However, the abundance of CaHira mRNA significantly decreased (P < 0.05) shortly after fertilization and then increased again and remained stable from gastrula till hatching. The varying spatiotemporal expression patterns of Hira genes in gyno-carp and gono-carp may be associated with the differing reproductive modes used by these two closely related fishes. Our results suggest that Hira may play a role not only in the decondensation of sperm nucleus and the formation of pronucleus during fertilization, but also in gastrulation and the subsequent development of embryos.
作者机构：
[Yang, X.; Lu, Z.; Qiao, Y.; Yan, Y.] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Lu, Z.; Li, C. M.; Qiao, Y.; Yan, Y.] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore, Singapore.
通讯机构：
[Yang, X.] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Formaldehyde;Learning;Memory;Morris water maze;NMDA receptor;Oxidative stress
摘要：
UNLABELLED: In this study, we investigated the effect of inhaled formaldehyde on learning and memory capacity. After exposure to 0 (control), 1 and 3 mg/m(3) of gaseous formaldehyde respectively, the behavior of mice in a Morris water maze, the expression of NR1, NR2B mRNA and oxidative damage levels in mice brain were analyzed. The water maze performance, the activities of dismutase superoxide (SOD) and levels of glutathione (GSH) decreased significantly in 3 mg/m(3) group (P < 0.01, compared with control group); while malondialdehyde (MDA) contents and expression of NR1 and NR2B genes increased significantly after exposure to 3 mg/m(3) of gaseous formaldehyde (P < 0.05, <0.01, <0.01, compared with control group). These findings indicate that inhaled formaldehyde negatively affects learning and memory at 3 mg/m(3) of gaseous formaldehyde but not at lower levels. Oxidative stress-induced neuron damages in the brain may be the possible mechanism for these effects. PRACTICAL IMPLICATIONS: This study indicates that inhaled formaldehyde starts to negatively affect learning and memory at a middle concentration of formaldehyde without interference of other indoor air pollutants. Oxidative damage, and the alteration of NMDA receptor expression, which were induced by formaldehyde inhalation, may be the possible mechanism for gaseous formaldehyde-induced neurotoxicity.
期刊：
CHINESE JOURNAL OF PHYSIOLOGY,2008年51(1):13-26 ISSN：0304-4920
通讯作者：
Wu, Fei-Jian
作者机构：
[Wu, Fei-Jian] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Jen, Philip H. -S.] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA.;[Jen, Philip H. -S.] Univ Missouri, Interdisciplinary Neurosci Program, Columbia, MO 65211 USA.
通讯机构：
[Wu, Fei-Jian] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
期刊：
Plant and Soil,2007年292(1-2):291-304 ISSN：0032-079X
通讯作者：
Ke, Wenshan
作者机构：
[Ke, Wenshan] Hubei Univ, Sch Life Sci, Wuhan 430062, Peoples R China.;Wuhan Univ, Dept Environm Sci, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Ke, Wenshan] H;Hubei Univ, Sch Life Sci, Wuhan 430062, Peoples R China.
摘要：
Copper accumulation, subcellular localization and ecophysiological responses to excess copper were investigated using pot culture experiments with two Daucus carota L. populations, from a copper mine and an uncontaminated field site, respectively. Significant differences of malondialdehyde (MDA) and hydrogen peroxide (H2O2) concentrations and antioxidant enzyme [superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX)] activities of leaves under Cu treatment were observed between the two populations. At high Cu concentrations (400 and 800mgkg−1), a significant increase in contents of MDA and H2O2 but a significant decrease in activities of SOD, CAT and APX were observed in uncontaminated population. Contrarily, the population from copper mine maintained a lower level of MDA and H2O2 but higher activities of SOD, CAT and APX. Copper accumulation in roots and shoots increased significantly with the increase of copper concentrations in soils in the two populations. No significant difference of the total Cu in roots and shoots was found between the two populations at same copper treatment. There were also no striking differences of cell wall-bound Cu and protoplasts Cu of leaves between the two populations. The difference was that Cu concentration in vacuoles of leaves was 1.5-fold higher in contaminated site (CS) population than in uncontaminated site population. Hence, more efficient vacuolar sequestration for Cu and maintaining high activities of SOD, CAT and APX in the CS population played an important role in maintaining high Cu tolerance.
摘要：
Myriophyllum oguraense Miki subsp. yangtzense D. Wang subsp. nova (Haloragaceae) from China, is described and illustrated. An identification key to the morphologically most similar taxa is provided. The new subspecies differs from M. oguraense subsp. oguraense by having large fruits and mericarps with two longitudinal ridges on the dorsal surface. The two taxa exhibit a disjunct distribution pattern, M. oguraense subsp. yangtzense being confined to the lower Yangtze valley in China, whereas M. oguraense subsp. oguraense is restricted to Japan.
作者机构：
[Wang, Hanzhong] Chinese Acad Sci, State Key Lab Virol, WIV, Wuhan, Peoples R China.;Cent China Normal Univ, Wuhan, Hubei, Peoples R China.;Xian Ning Ctr Dis Control & Prevent, Xian Ning, Hubei, Peoples R China.;Chinese Acad Sci, Huazhong Normal Univ, Coll Life Sci, Wuhan, Peoples R China.
通讯机构：
[Wang, Hanzhong] C;Chinese Acad Sci, State Key Lab Virol, WIV, Wuhan, Peoples R China.
摘要：
Of 322 stool specimens collected from children with diarrhea from October 2005 through September 2006 in Wuhan, China, group A rotavirus was identified in 101 (31.4%). The most prevalent group A rotavirus genotype was G3P[8] (62.6%), followed by G1P[8](17.6%), G1+G3P[8](8.8%), G3P[4](6.6%), G1P[4](2.2%), and G9P[8](2.2%). The G9 strains were first detected in Wuhan.
摘要：
Effects of 111 days enhanced UV-B radiation on leaf pigments, grain quality, photosynthesis and growth were determined in Chinese superhigh-yield hybrid rice (Oryza sativa L.) Liangyoupeijiu (Peiai 64S × 9311). The chlorophyll concentrations of leaves under enhanced UV-B radiation were slightly reduced in the tillering stage, but recovered to levels equal to or above those of control in the later growth stages. Leaves treated with enhanced UV-B radiation for 99 days exhibited higher absorbance in the UV region than controls. Amylose concentration of UV-B treatment was significantly increased by 4.1%. Crude fat concentration, crude protein concentration and vitamin B1 showed no significant difference between control and treatment. The light-saturated photosynthetic activity of UV-B treated leaves normalized on fresh weight or chlorophyll concentration was, respectively, 45.2 and 35.3% higher than controls. Both indoor and field experiments indicated that UV-B treated leaves were more tolerant to photoinhibition, and its Do values tended to be higher than controls during the strong light period of midday. The D1 protein turnover played more important role in UV-B treated leaves against strong irradiance than in control leaves. Little difference was observed in the maximal PSII quantum efficiency of UV-B treated and control leaves during the process of leaf senescence. UV-B radiation reduced diseased grains per panicle and led to a higher seed setting rate. It may have a direct harmful effect on pathogens or reduced susceptibility of UV-B exposed plants to pathogens. Our results suggest that long-term exposure to enhanced UV-B radiation is unlikely to have detrimental effects on the photosynthetic capability of Liangyoupeijiu, and tends to inhibit its growth and biomass.
