摘要：
The functional mevalonate pathway of the Class-II HMG-CoA reductase (Class-II HMGR) from bacterial pathogens is essential for the survival of these pathogens. So, Class-II HMGR has been identified as one of the most important targets of antibiotics, which could be used to treat infections caused by these pathogens. With the aim of designing novel inhibitors of Class-II HMGR, we created a theoretical model of the three-dimensional dimmer structure of Class-II HMGR from Streptococcus pneumoniae by using homology modeling and molecular dynamic simulation procedures. Subsequently, we analyzed ligand–receptor interactions by docking lovastatin and HMG into the proposed three-dimensional dimmer structure. Results revealed that the binding model of lovastatin and HMG were similar to the binding model in their crystal structure. These results further confirmed the reliability of the homology model of Class-II HMGR, and also showed that the selected parameter and method for the docking process were reasonable. In the absence of a crystal structure, our study provides an early insight into the structure and its binding to the substrate and ligand, thus providing a new starting point for the identification or rational design of more potent inhibitors.
期刊：
JOURNAL OF EXPERIMENTAL BOTANY,2007年58(12):3227-3238 ISSN：0022-0957
通讯作者：
Li, Xue-Bao
作者机构：
[Huang, Geng-Qing; Li, Li; Li, Xue-Bao; Wang, Xiu-Lan] Huazhong Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Li, Xue-Bao] H;Huazhong Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
关键词：
Cotton;"expression profiles of GhTUA genes";"fibre elongation";fibre-specific;microtubule;"overexpression in yeast cells"
摘要：
The microtubule cytoskeleton may play an important role in the polarized growth of fibre cells that are single-cell trichomes on the surface of cotton ovules. To investigate whether the high expression levels of α-tubulin genes are correlated with fibre elongation, nine GhTUA genes (cDNAs) encoding α-tubulins with 449–451 amino acid residues were isolated and characterized in cotton. The GhTUA genes share high sequence homology at the nucleotide level (62–93% identity) in the coding region and at the amino acid level (89–99% identity), and can be classified into two subgroups. Real-time quantitative RT-PCR analysis revealed that seven out of the nine GhTUA genes are predominantly expressed in developing fibres. Among them, GhTUA9 displays the highest level of expression, revealing its fibre specificity. The GhTUA9 transcripts in fibres reached its peak value between 5–10 DPA, and dramatically declined to undetectable levels as the ovule matured further, suggesting that its expression is developmentally-regulated in fibres. The GhTUA9 gene including the promoter region was isolated from the cotton genome. To demonstrate the specificity of the GhTUA9 promoter, the 5′-flanking region, including the promoter and 5′-untranslated region, was fused with the GUS gene. Histochemical assays demonstrated that the GhTUA9:GUS gene was specifically expressed in elongating fibres. Overexpression of GhTUA9 in fission yeast (Schizosaccharomyces pombe) promoted atypical longitudinal growth of the host cells by 1.4–1.7-fold, indicating that the GhTUA9 gene is involved in cell elongation. Given all the above results, it is proposed that the GhTUA9 gene may play an important role in fibre elongation.
作者机构：
[Wang, Xuedong] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, Xuedong] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
作者机构：
[Jen, Philip H. -S.] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA.;Univ Missouri, Interdisciplinary Neurosci Program, Columbia, MO 65211 USA.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Jen, Philip H. -S.] U;Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA.
摘要：
The small molecule, meso-tetra(alpha,alpha,alpha,alpha-o-phenylacetamidophenyl) porphyrin (Mr1147.0) was used as complete antigen to elicit MAb through the immunization and cell fusion techniques. The MAb 1F2 obtained was demonstrated to be very pure by MALDI/TOFMS. The subtype of MAb 1F2 is IgG2a, which has a relative molecular weight of 156,678.8 Da. No significant change in the intensity of absorption peaks in UV and CD spectra was observed over a pH range between 6 and 12. The high stability of the abzyme and the tight binding between Fe porphyrin and antibody were also demonstrated. V-max, K-m, kappa(cat), kappa(cat)/K-m for abzyme are 5.18 x 10- 8 Ms- 1, 1.50 x 10(-8) Ms--1,Ms- 0.518 s(-1), 3.45 x 10(7) M(-1)s(-1), respectively. The data obtained indicate that catalytic antibody has high catalytic activity. The chloroperoxidase activity of MAb 1F2-Fe porphyrin complex is stable from 10 degrees C to 60 degrees C.
摘要：
Crystal structures of the L3MBTL1 MBT repeats in complex with histone H4 peptides dimethylated on Lys20 (H4K20me2) show that only the second of the three MBT repeats can bind mono- and dimethylated histone peptides. Its binding pocket has similarities to that of 53BP1 and is able to recognize the degree of histone lysine methylation. An unexpected mode of peptide-mediated dimerization suggests a possible mechanism for chromatin compaction by L3MBTL1.
期刊：
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy,2007年26(2):62-65 ISSN：1554-0014
通讯作者：
Wang, Fengyang
作者机构：
Hainan Univ, Coll Life Sci & Agr, Haikou 570228, Peoples R China.;Huazhong Normal Univ, Coll Life Sci, Wuhan, Peoples R China.;[Wang, Fengyang] Hainan Univ, Coll Life Sci & Agr, Renmin Rd 58, Haikou 570228, Peoples R China.
通讯机构：
[Wang, Fengyang] H;Hainan Univ, Coll Life Sci & Agr, Renmin Rd 58, Haikou 570228, Peoples R China.
摘要：
The small molecule meso-Tetra (α,α,α,α-o-phenylacetamide benzene) porphyrin was synthesized through the condensation of o-nitrobenzaldehyde and pyrrole followed by reduction of the meso-tetra (o-nitrophenyl) porphyrin. The small molecule, without carrier, was used as complete antigen to immunize BALB/ C mice. Spleen cells producing high titer antibody were removed and fused with myeloma cells of SP2/0 origin. Using a conventional immunization protocol, stable murine monoclonal antibodies (MAbs) producing cell lines to meso-Tetra (α,α,α,α-o-phenylacetamide benzene) porphyrin 1F2 were obtained. Subclass determination showed that the clones produce IgG2a types of MAbs. The analytical results of HPLC and MALDI/TOFMS suggest that the purity of MAb 1F2 is 100%, and MAb 1F2 has a relative molecular weight of 156678.8 Da. Our results demonstrated that small molecule meso-Tetra (α,α,α,α-o-phenylacetamide benzene) porphyrin, as semiantigen without carrier, can elicit the formation of MAbs.
作者机构：
[Yang, Shao] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Yang, Shao] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
期刊：
Bulletin of Environmental Contamination and Toxicology,2007年78(1):61-65 ISSN：0007-4861
通讯作者：
Wang, X.-D.
作者机构：
[Wang, X.-D.] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, X.-D.] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Biosorption;Algal Cell;Anhydrous Sodium Sulfate;Relative Standard Deviation;Organophosphorus Pesticide
作者机构：
[Peng, Jianxin] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Inst Entomol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Peng, Jianxin] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
关键词：
AfMNPV;Caspase 3;Cyclosporin A;Cytochrome c;Spodoptera litura cell line (SL-ZSU-1, SL-1)
摘要：
We investigated the influence of cytochrome c on apoptosis induced by Anagrapha (Syngrapha) falcifera multiple nuclear polyhedrosis virus (AfMNPV). Microscopic observation revealed that infection of SL-1 cells with AfMNPV resulted in apoptosis, displaying apoptotic bodies in fluorescent-stained nuclei of AfMNPV-infected SL-1cells. Western blot analysis demonstrated that AfMNPV-induced apoptosis in insect SL-1 cells was significantly inhibited by cyclosporin A which blocked a translocation of cytochrome c from the mitochondria to the cytosol. As determined by using AC-DEVD-AFC as substrate, the activity of caspase-3 in AfMNPV-induced cells was detected as early as 4 h post infection, gradually increased with time extension, and reached a highest level after 16 h of infection. However, activity of caspase-3 in apoptotic cells decreased in the presence of cyclosporin A (30 mu M), indicating that activation of caspase-3 in SfaMNPV-induced cells was dependent on the release of cytochrome c from the mitochondria. In addition, cyclosporin A could markedly inhibit mitochondrial transmembrane potential (Delta Psi m) disruption in undergoing apoptotic cells. These data indicate that cytochrome c plays a key role in AfMNPV-induced apoptosis in S. litura cells and may be required for caspase activation during the induction of apoptosis. (C) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
期刊：
JOURNAL OF SEPARATION SCIENCE,2007年30(15):2506-2512 ISSN：1615-9306
通讯作者：
Wang, Xuedong
作者机构：
[Wang, Xuedong] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, Xuedong] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
作者：
Jia Tang;Fei-Jian Wu;Dan Wang;Jen, Philip H. -S.;Qi-Cai Chen*
期刊：
CHINESE JOURNAL OF PHYSIOLOGY,2007年50(4):187-198 ISSN：0304-4920
通讯作者：
Qi-Cai Chen
作者机构：
[Qi-Cai Chen] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA.;Univ Missouri, Interdisciplinary Neurosci Program, Columbia, MO 65211 USA.
通讯机构：
[Qi-Cai Chen] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
Natural auditory environment consists of multiple sound sources that are embedded in ambient strong and weak noise. For effective sound communication and signal analysis, animals must somehow extract biologically relevant signals from the inevitable interference of ambient noise. The present study examined how a weak noise may affect the amplitude sensitivity of neurons in the mouse central nucleus of the inferior colliculus (IC) which receives convergent excitatory and inhibitory inputs from both lower and higher auditory centers. Specifically, we studied the amplitude sensitivity of IC neurons using a probe (best frequency pulse) and a masker (weak noise) under simultaneous masking paradigm. For most IC neurons, weak noise masking increases the minimum threshold and decreases the number of impulses. Noise masking also increased the slope and decreased the dynamic range of the rate amplitude function of these IC neurons. The strength of this noise masking was greater at low than at high sound amplitudes. This variation in the amplitude sensitivity of IC neurons in the presence of the weak noise was mostly mediated through GABAergic inhibition. These data indicate that in the real world the ambient weak noise improves amplitude sensitivity of IC neurons through GABAergic inhibition while inevitably decreases the range of overall auditory sensitivity of IC neurons.
期刊：
Bulletin of Environmental Contamination and Toxicology,2007年78(5):368-372 ISSN：0007-4861
通讯作者：
Wang, X.-D.
作者机构：
[Wang, X.-D.] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Col Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, X.-D.] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
期刊：
Pest Management Science,2007年63(3):296-300 ISSN：1526-498X
通讯作者：
Peng, Jianxin
作者机构：
[Peng, Jianxin] Cent China Normal Univ, Inst Entomol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Peng, Jianxin] C;Cent China Normal Univ, Inst Entomol, Wuhan 430079, Peoples R China.
关键词：
Spodoptera exigua;Syngrapha falcifera multiple nucleopolyhedrovirus;peritrophic membrane;environmental scanning electron microscope;Calcofluor white M2R
作者机构：
[Zhang, Shu-Yi] E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China.;Chinese Acad Sci, Inst Zool, Beijing 100080, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;Univ Calif Los Angeles, Dept Physiol Sci, Los Angeles, CA 90095 USA.;Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China.
通讯机构：
[Zhang, Shu-Yi] E;E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China.
摘要：
Echolocation calls and neurophysiological correlations with auditory response properties in the inferior colliculus of Pipistrellus abramus (Microchiroptera: Vespertilionidae). Zoological Studies 46(5): 622-630. The present study examines the echolocation calls and auditory responses of single neurons in the inferior colliculus (IC) of Pipistrellus abramus (Microchiroptera: Vespertilionidae). The data showed that there was a neurophysiological correlation of the auditory response properties with echolocation calls in IC neurons. The echolocation calls of P. abramus were broad-band swept from 86.6 to 43.2 kHz. The ending frequencies of the first harmonics which centered around 40 (average, 43.2; range, 37.0-47.0) kHz, were relatively more stable than the initial high frequencies. The average peak frequency was 52.1 (range, 43.3-57.6) kHz of which the majority (81%, 154 of 190 calls) ranged from 50.1 to 60 kHz. We recorded the responses of 75 single IC neurons to pure tones. Most IC neurons had the best frequency (BF) at between 30 and 50 kHz (centered around 40 kHz) (73%, 54 of 75) and between 50.1 and 60 kHz (19%, 14 of 75), respectively corresponding to the ending frequencies of the first harmonics and peak frequencies. The minimum threshold (MT) distribution was wider, and the average MT was significantly higher for neurons with a BF of 30-50 kHz than for neurons with a BF of 50.1-60 kHz (62 +/- 11 vs. 49 +/- 8 dB SPL, p < 0.001, t-test). The latency distribution was also slightly wider for neurons with a BF of 30-50 kHz (71% between 6.1 and 8.0 ms) than for neurons with a BF of 50.1-60 kHz (79% between 4.0 and 6.0 ms). Our study of echolocation calls and auditory response properties of IC neurons suggests that the IC of P. abramus can effectively process emitted pulses and echoes during hunting. http://zoolstud.sinica.edu.tw/Journals/46.5/622.pdf.
