作者机构：
[Liu, Kaiyu] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Liu, Kaiyu] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
作者机构：
[Zheng, Yonglian] Huazhong Normal Univ, Coll Life Sci & Technol, State Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China.;Huazhong Normal Univ, Coll Life Sci & Technol, Natl Ctr Crop Mol Breeding, Wuhan 430070, Peoples R China.
通讯机构：
[Zheng, Yonglian] H;Huazhong Normal Univ, Coll Life Sci & Technol, State Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China.
关键词：
cytoplasmic male sterility;mitochondria;mRNA decay;stem‐loop;polyadenylation
摘要：
The co-transcribed orf355-orf77 region of the mitochondrial genome is associated with S cytoplasmic male sterility (CMS-S) in maize; the amounts of its 1.6- and 2.8-kb transcripts were previously shown to be greatly reduced in fertility-restored microspores relative to the amounts in sterile plants. To investigate the mechanism underlying this reduction, detailed analysis of the 5' and 3' termini of these transcripts was conducted. Using 3' RACE analysis, the polyadenylation sites of the 1.6- and 2.8-kb transcripts were mapped adjacent to a 3' stem-loop, which may play an important role in stabilizing their 3' ends. No difference was found between the polyadenylation sites in sterile and fertility-restored microspores that could account for the differences in orf355-orf77 transcript levels. The 5' terminus of the 1.6-kb transcript was further studied by primer extension; the result revealed that there was a deletion of nine nucleotides only in fertility-restored microspores, and that this deletion eliminated a 5' stem-loop sequence. We propose that the elimination of the 5' stem-loop in the fertility-restored microspores could be the cause of the degradation of the 1.6-kb transcript. Because the 2.8-kb transcript can be cleaved to generate the 1.6-kb transcript, the amount of the 2.8-kb transcript is also reduced in fertility-restored microspores.
摘要：
Formaldehyde (FA) is a genotoxic and mutagenic substance. In 2004, IARC (International Agency for Research on Cancer) concluded that FA is carcinogenic in humans after reevaluating the available evidence on the carcinogenicity of FA. Although many studies have shown that FA had extensive genotoxicity including DNA-protein crosslinks (DPC) and DNA single strand breaks (DSSB), most of these studies only discussed the effects of FA at high levels. In this study, KCl-SDS assay and single cell gel electrophoresis (SCGE) were used to investigate the formation and repair process of FA-induced DPC and DSSB in human peripheral blood lymphocytes and Hela cell lines. KCl-SDS assay was applied to detect DPC induced by liquid FA in human peripheral blood lymphocytes in vitro. The results showed that FA could induce DPC at high levels (≥50 micro M). By combining the results of KCl-SDS assay and SCGE, it could be determined that FA would induce DNA-DNA crosslinks (DDC) when FA concentration was more than 25 microM. The repair process of FA-induced DPC was studied with KCl-SDS assay in Hela cell lines and the results demonstrated that FA-induced DPC could be significantly repaired after 18 hours. The SCGE was also used to determine FA-induced DSSB and its repair process in Hela cell lines. The results demonstrated that DNA breakages, which is capable of being induced by FA at a low level (<30 microM), enabled to be repaired completely in 90 minutes.
作者：
Li Ping;Liu De-li*;Nahimana Liberat;Chen Shu-li;Yang Xi;...
期刊：
环境科学学报(英文版),2006年18(3):525-529 ISSN：1001-0742
通讯作者：
Liu De-li
作者机构：
[Li Ping; Chen Shu-li; Liu De-li; Yang Xi; Zhao Li; Liu, DL; Nahimana Liberat] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Liu De-li] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
关键词：
high nitrogen removal;aerobic denitrification;wastewater treatment;identification
摘要：
Three new bacteria HS-03, HS-043 and HS-047 isolated from different ecosystems were found capable of aerobic denitrification. The potential application of these strains in wastewater treatment under aerobic conditions was investigated. These three bacteria all presented high nitrogen removal from wastewater that more than 98% of 10 mmol/L nitrate could be removed in 12-24 h by adding cheap external carbon source and low concentration of iron as well as molybdate. The mechanism at molecular level was analyzed. The success of this aerobic denitrification applied to wastewater treatment may serve as an alternative to enhance the practical nitrogen removal from wastewater. Main biochemical and physiological features of these strains were characterized. The 16S rDNA sequences were compared with the published data in GenBank by using BLAST. The results of phenotype and genotype proved that strain HS-03 and HS-047 belonged to Pseudomonas stutzeri and Pseudomonas pseudoalcaligenes respectively. Strain HS-043 was identified as Delftia acidovorans of which denitrifying activity has not previously been explored.
作者：
Wang, Xu;Douglas, Steven D.;Peng, Jin-Song;Zhou, Dun-Jin;Wan, Qi;...
期刊：
AMERICAN JOURNAL OF PATHOLOGY,2006年169(5):1663-1670 ISSN：0002-9440
通讯作者：
Ho, Wen-Zhe
作者机构：
Univ Penn, Childrens Hosp Philadelphia, Sch Med,Joseph Stokes Jr Res Inst,Dept Pediat, Div Allergy & Immunol,Abramson Res Ctr, Philadelphia, PA 19104 USA.;Wuhan Ctr Dis Prevent & Control, Virol Lab, Wuhan, Peoples R China.;Wuhan Univ, Renmin Hosp, Dept Pediat, Wuhan 430072, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan, Peoples R China.;[Ho, Wen-Zhe] Univ Penn, Childrens Hosp Philadelphia, Sch Med,Joseph Stokes Jr Res Inst,Dept Pediat, Div Allergy & Immunol,Abramson Res Ctr, Room 1202B,34th St & Civ Ctr Blvd, Philadelphia, PA 19104 USA.
通讯机构：
[Ho, Wen-Zhe] U;Univ Penn, Childrens Hosp Philadelphia, Sch Med,Joseph Stokes Jr Res Inst,Dept Pediat, Div Allergy & Immunol,Abramson Res Ctr, Room 1202B,34th St & Civ Ctr Blvd, Philadelphia, PA 19104 USA.
作者机构：
[Liu, DL] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hebei, Peoples R China.;Huazhong Univ, Minist Educ China, Key Lab Pesticide & Chem Biol, Wuhan, Peoples R China.;Univ Tokyo, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan.
通讯机构：
[Liu, DL] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hebei, Peoples R China.
关键词：
small interfering RNA;adhesion molecule CD146;vascular endothelial cell
摘要：
Our previous study has demonstrated that CD146 molecule is a biomarker on vascular endothelium, which is involved in angiogenesis and tumor growth. However the mechanism behind is not clear. Here we have for the first time developed a novel CD146 blockade system using CD146 siRNA to study its function on endothelial cells. Our data showed that CD146 siRNA specifically blocked the expression of CD146 on both mRNA and protein levels, leading to the significant suppression of HUVEC proliferation, adhesion and migration. These results demonstrate that CD146 plays a key role in vascular endothelial cell activity and angiogenesis, and CD146 siRNA can be used as a new inhibitor for anti-angiogenesis therapy.
摘要：
B.S. Qiu and Y. Li. 2006. Photosynthetic acclimation and photoprotective mechanism of Haematococcus pluvialis (Chlorophyceae) during the accumulation of secondary carotenoids at elevated irradiation. Phycologia 45: 117–126. DOI: 10.2216/04-99.1 The photosynthetic acclimation of Haematococcus pluvialis Flotow to elevated irradiance and its photoprotective mechanisms were investigated. High light caused a remarkable increment in carotenoid content per cell. Cellular and volumetric chlorophyll contents were significantly increased after four days of high light treatment. Net photosynthesis of high light treated cells was decreased but their dark respiration was increased during the accumulation of secondary carotenoids (SC). The inactivation of reaction centers was observed in high light treated cells, and their normalized complementary area and turnover number were higher than those under low light. The PS II activity of red cells from high light was decreased by 17% compared with green cells from low light but their PS I activity was significantly increased. The K-step could not be observed in the fluorescence transients of red cells, indicating that the oxygen-evolving complex was not affected during SC accumulation. Haematococcus pluvialis could protect itself against strong irradiance through the D1 protein repair cycle and the xanthophyll cycle. The D1 protein repair cycle was the most important protective mechanism in H. pluvialis and its operation could alleviate photoinhibition by 49% in green cells and by 53~55% in red cells. The xanthophyll cycle could contribute to the protection of green cells subjected to strong irradiance but its role was negligible in red cells. Fv /Fo values were decreased in green cells and red cells by 45% and 32~34% respectively after 2.5 hours of photoinhibitory treatment. However, this may not necessarily indicate that the accumulated SC in red cells might play a photoprotective role.
期刊：
Molecular Genetics and Metabolism,2006年88(4):295-306 ISSN：1096-7192
通讯作者：
Huang, QY
作者机构：
[Huang, Qing-Yang] Department of Medicine, The University of Hong Kong, Hong Kong, PR China<&wdkj&>College of Life Sciences, Central China Normal University, Wuhan, Hubei 430079, PR China;[Kung, Annie Wai Chee] Department of Medicine, The University of Hong Kong, Hong Kong, PR China
通讯机构：
[Huang, QY ] ;Univ Hong Kong, Dept Med, Hong Kong, Hong Kong, Peoples R China.
关键词：
genetics;bone mineral density;osteoporosis;linkage;association
摘要：
Osteoporosis is a common disease with a strong genetic component. In recent years, some progress has been made in understanding the genetic basis of osteoporosis. Genetic factors contribute to osteoporosis by influencing not only bone mineral density but also bone size, bone quality, and bone turnover. Meta-analysis has been used to define the role of several candidate genes in osteoporosis. Some quantitative trait loci that regulate bone mass identified by linkage studies in humans and experimental animals have been replicated in multiple populations. Genes that cause monogenic bone diseases also contribute to regulation of bone mass in the normal population. Genome-wide association studies and functional genomics approaches have recently begun to apply to genetic studies of osteoporosis. In the future, not only single gene but also the entire gene networks involved in osteoporosis and regulation of bone mass will systematically be discovered through integrative genomics. (c) 2006 Elsevier Inc. All rights reserved.
期刊：
Journal of Bone and Mineral Metabolism,2006年24(2):132-137 ISSN：0914-8779
通讯作者：
Deng, HW
作者机构：
Creighton Univ, Osteoporosis Res Ctr, Omaha, NE 68131 USA.;Cent China Normal Univ, Coll Life Sci, Hubei, Peoples R China.;Hunan Normal Univ, Coll Life Sci, Hunan, Peoples R China.;Xian Jiaotong Univ, Key Lab Biomed Informat Engn, Minist Educ, Xian 710049, Peoples R China.;Xian Jiaotong Univ, Inst Mol Genet, Sch Life Sci & Technol, Xian 710049, Peoples R China.
通讯机构：
[Deng, HW] C;Creighton Univ, Osteoporosis Res Ctr, 601 N 30th St,Suite 6787, Omaha, NE 68131 USA.
摘要：
Osteoporosis has a strong genetic component, but the genes involved are poorly defined. Genome-wide scans in multiple populations have identified chromosome 1p36 as one region linked to bone mineral density (BMD). The tumor necrosis factor receptor 2 (TNFR2) at 1p36 is a positional and functional candidate gene in osteoporosis. In this study, we conducted linkage and association tests between the CA repeat polymorphism of the TNFR2 gene and BMD in two large independent samples using the quantitative transmission disequilibrium test (QTDT) program. The first group of subjects was composed of 1836 individuals from 79 multigeneration pedigrees. The second group was a randomly ascertained set of 636 individuals from 157 nuclear families. We found no evidence of association or linkage for spine or hip BMD in the samples of the multigenerational pedigrees or nuclear families. Through testing for association and for linkage, our data do not support the TNFR2 gene as a QTL underlying hip or spine BMD variation in our Caucasian populations.
摘要：
Abstract Copper is an important ingredient in fungicides, which are used for many economic plants. However, there is concern about the side‐effects of copper‐based fungicides due to their potential to affect beneficial mites. The purpose of the present paper was to investigate the effects of copper on the development of the fruit fly, Drosophila melanogaster. It was found that higher doses of copper significantly prolonged the developmental time of the fruit flies, especially during the larval stages. When 320 mg L‐1 copper‐contaminated food was used, most of the larvae died when they were small and before the pupal stage. The protein contents and esterase isozymes extracted from the larvae changed according to the doses of copper. The small‐molecular‐weight protein bands gradually became weaker or were lost as the copper levels increased. However, low doses of copper stimulated a stronger expression of a few proteins. These results indicate that low doses of copper generally have no lethal effects on D. melanogaster because a specific group of genes, which encode specific proteins, are probably activated in order to withstand the onslaught of stressful conditions. At high doses of copper in food (e.g. 320 mg L‐1), fly development and viability are significantly affected.
摘要：
The difference of sensitivity to indole-3-acetic acid (IAA) combined with horseradish peroxidase (HRP) in K562 and BXPC-3 cells was investigated. The cell proliferation was determined by MTT assay. The cell cycle and apoptosis of K562 and BXPC-3 cells were examined by a fluorescence flow cytometer (FCM) and terminal deoxynucleotidyl transferase-mediated dUTP nickrend labeling (TUNEL) respectively. The experimental results show that IAA and HRP could inhibit BXPC-3 cell proliferation greatly compared with K562 cell during the first 48 h. The cell cycle was arrested predominantly at G2/M phase in K562 and BXPC-3 cells. The cell apoptosis of K562 and BXPC-3 was induced by IAA/HRP. There was a significant difference between the two cell lines since BXPC-3 cells were more sensitive than K562 cells by treatments with combination of IAA and HRP.
作者机构：
[Chen, XW] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China.;Cent China Normal Univ, Inst Entomol, Wuhan 430070, Peoples R China.;Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China.
通讯机构：
[Chen, XW] C;Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China.
关键词：
HaSNPV;p78/83;Ha2;WASP;baculovirus;structural protein
摘要：
The open reading frame 2 (ha2) of the Helicoverpa armigera single nucleocaspid nucleopolyhedrovirus (HaSNPV), a conserved gene in 111ost baculoviruses from lepidopteran insects such as p78/83 of the Autographa californica MNPV, was characterized. It is 1242 bp long and potentially encodes a 45.9 kDa. Ha2 is conserved among baculoviruses from lepicdopteran insects. Ha2 transcripts were detected front 16 to 96 11 post infection (hpi) of HzAM1 cells. Rabbit polyclonal antiserum against a GST-HA2 fusion protein reacted with three protein of 50, 46 and 35 kDa at 24-72 hpi of HzAM1 cells. Anti OpMNPV ORF2 (homologue of HA2) antibody reacted only with the 46 and 35 kDa proteins in HaSNPV-infected cells. These results demonstrate that Ha2 is modified at the mRNA or protein levels. Western blot analysis showed that only the 50kDa product of HA2 is a structural component of proteins of both the budded virus (BV) and occlusion-derived virus (ODV) phenotypes. HA2-EGFP fusion protein showed that HA2 is localized primarily in the nucleus of HzAM1 infected cells. The HA2 was found to co-localize with actin by labelling of actin with Rhordamine-Phalloidin. In summary, the data indicated that HA2 is a structural protein and interacts with host cell actin. (c) 2005 Elsevier B.V. All rights reserved.
作者机构：
[Liu, Kaiyu; Peng, Jianxin; Peng, Rong; Hong, Huazhu; Hong, HZ; Yang, Hong; Zheng, Jin] Cent China Normal Univ, Inst Entomol, Wuhan 430079, Peoples R China.
通讯机构：
[Hong, Huazhu] C;Cent China Normal Univ, Inst Entomol, Wuhan 430079, Peoples R China.
关键词：
Bacillus thuringiensis;Chemical pesticide;Esterase;Osmotic lysis;Resistance;Trichoplusia ni cell line
摘要：
Cabbage looper moth (Trichoplusia ni) cell line BTI-Tn-5B1-4 (TnH5) has developed high-level resistance (>1000 fold) by the selection of Bt Cry1Ac10 toxin. In order to examine mechanisms of resistance to Cry1Ac10 toxin (biological pesticide), both general esterase activities and cell tolerance to osmotic lysis were compared between non-selected Cry1Ac10-susceptible Trichoplusia ni cell line TnH5-S and Cry1Ac10-resistant Trichoplusia ni cell line TnH5-R selected by Bt Cry1Ac10. The Cry1Ac10-resistant TnH5-R cells had lower general esterase activity than the non-selected TnH5-S cells, and the esterase isozyme bands for the Cry1Ac10-resistant TnH5-R cells were much weaker than that for the non-selected TnH5-S cells. Both activated Cry1Ac10 toxin and multi-toxin from Bacillus thuringiensis subsp. aizawai GC-91 (an engineering bacterium) could not inhibit the esterase activity both in the Cry1Ac10-susceptible and Cry1Ac10-resistant cells, but two chemical pesticides, chlopyrifos and methomyl, could greatly inhibit the esterase activities both in the TnH5-R and TnH5-S cells. On the other hand, cell tolerance to osmotic lysis caused by hypotonic solution for the Cry1Ac10-resistant TnH5-R cells was higher than that for the non-selected TnH5-S cells (2.5×). Based on these results, we made the following conclusions. The general esterase activities in the Cry1Ac10-resistant TnH5-R cells was not related to Bt Cry1Ac10 resistance, but the susceptibility to the two tested chemical pesticides increased in TnH5-R cells because of their lower esterase activity. The increase of cell tolerance to osmotic lysis for the Cry1Ac10-resistant TnH5-R cells may be one of the mechanisms for Bt toxin resistance because midgut cells of insects are also disrupted by an osmotic lysis caused by Bt toxin.
摘要：
A simple and sensitive electrochemical immunosensor with impedance labelless detection and novel data processing method was investigated. One-step copolymerization was used to electrochemically deposit an antibody impregnated polypyrrole film on a glassy carbon electrode surface for the immunosensor. Impedance measurements provided a labelless or reporterless method to detect antibody (Ab)-antigen (Ag) interactions. Dimensionless analysis was employed to successfully process the measured impedance data. Since the method derived unit impedance change to eliminate or reduce the variation of the bulk electronic properties of Ab/polypyrrole films, the signal to noise ratio (S/N) was significantly improved for high sensitivity and specificity. Nonspecific binding effect was studied by array electrode chips and was found out that the polypyrrole electrode without antibody attachment had much stronger nonspecific binding effect than the Ab/polypyrrole electrode; incubation followed by thoroughly washing significantly reduced the nonspecific interference. 10 pg/ml detection limit and superior specificity were achieved by the method, demonstrating a highly sensitive labelless immunosensor in comparison with the detection limit of ng -microgram/ml for the reported polypyrrole based immunosensors. The electrochemical immunosensors presented in this paper, due to its simplicity, low cost, high sensitivity and superior specificity, could be an invaluable tool for clinical diagnostics and could have potential applications in drug discovery, environmental and food analysis.
摘要：
The prevalence of asthma keeps on increasing worldwide, especially in western societies over last 40 years. The mechanism of asthma is unclear. Recently, concern about indoor air pollution as a risk factor for asthma has been arisen. In present study, 25 Kun Ming male mice were placed in an air chamber containing respective formaldehyde ( FA) concentration of 0, 0.5, 1.0, 3.0 mg/m(3), and 3.0 mg/m3 with Capsazepine ( CPZ, a specific antagonist of vanilloid receptor)-pretreatment in five testing groups ( n= 5 per group) for inhale experiments. The inhaled groups were exposed to gaseous FA for 6 hours each day in 10 successive days. After exposure, the concentrations of IL4 in blood serum and broncho alveolar lavage fluid (BALF) were measured. Experimental results showed that the IL4 level in serum was too low to be detected; and the concentrations of IL4 in BALF increased in a dose-dependent manner. However, for the CPZ-pretreated group the IL4 level in BALF decreased significantly ( compared with 3.0 mg/m(3) FA inhaled group, p < 0.01). This paper describes experimental animal methods to probe IL4 level, an important indicator for IgE response. The studies in this paper indicated that gaseous FA might induce acquired atopy by type II VR1 signaling system. These findings suggested that indoor air pollutants such as FA might be key risk factors for the rise in asthma cases, and type II VR1 signaling system might be one of the mechanisms for the rise.
