摘要：
In order to explore the molecular regulation of GSNO reductase (GSNOR), effects of environmental formaldehyde exposure and antioxidant alpha-lipoic acid (LA) injection on GSNOR activity and expression in mice lungs were investigated in the present study. Results showed that GSNOR activity was elevated upon 3.0 mg/m(3) FA exposure (compared with 0 mg/m(3) FA inhaled group, p<0.01) and the elevation was completely inhibited by LA injection (compared with 3.0 mg/m(3) FA inhaled group, p<0.01). Meanwhile, GSH levels were significantly decreased in 3.0 mg/m(3) FA inhaled group (compared with 0 mg/m(3) FA inhaled group, p<0.01), and were regenerated by LA injection (compared with 3.0 mg/m(3) FA inhaled group, p<0.01). The expression of GSNOR by using RT-PCR method correlated with the results of GSNOR activity assay. The results indicated that gaseous FA might activate GSNOR, and the activation may be related with the increase of oxidative stress. Since GSNOR has a central role in mediating SNO signaling and asthma development, these findings also suggested that indoor air pollutants such as FA might be key risk factors for the rise in asthma cases.
期刊：
BIOTECHNOLOGY AND SUSTAINABLE AGRICULTURE 2006 AND BEYOND,2007年:295-298
通讯作者：
Li, Xue-Bao
作者机构：
[Wang, Xiu-Lan; Wang, Hong; Li, Xue-Bao; Xu, Wen-Liang; Shi, Hai-Yan; Li, Deng-Di] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Li, Xue-Bao] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
14-3-3 protein was originally identified during a systematic classification of bovine brain proteins. Thereafter, a number of 14-3-3 proteins have been found in every eukaryotic organism such as animal, fungi, and plant species. In combination with signal proteins, the 14-3-3s prove to contribute to the regulation of many crucial cellular processes including metabolism, signal transduction, cell-cycle control, apoptosis, protein trafficking, transcription, stress responses, malignant transformation, as well as membrane-transportation of mitochondrion and chloroplast precursor proteins. 14-3-3 proteins are encoded by a multigene family in both animals and plants. Arabidopsis contains 13 functional genes encoding typical 14-3-3 proteins. The 14-3-3 genes have been shown to be expressed in cell or tissue specific manners during Arabidopsis development. Three 14-3-3 genes have been isolated in barley. In potato, up- or down-regulation of the 14-3-3 gene expression altered the composition of lipids, amino acids, and minerals. Furthermore, over-expression of 14-3-3 gene enhances antioxidant activity by 45%, and consequently, delays leaf senescence in potato. Although 14-3-3 genes in a few plant species, such as Arabidopsis, have been well characterized, little is known on cotton (Gossypium hirsutum) 14-3-3 gene, especially its role in regulation of fiber cell elongation. In this study, a cDNA encoding a putative 14-3-3 protein was isolated from fiber cDNA library of cotton. Here, we reported the molecular characterization of the Gh14-3-3L gene and its expression profile in cotton.
作者机构：
[Wang, Hanzhong] Chinese Acad Sci, State Key Lab Virol, WIV, Wuhan, Peoples R China.;Cent China Normal Univ, Wuhan, Hubei, Peoples R China.;Xian Ning Ctr Dis Control & Prevent, Xian Ning, Hubei, Peoples R China.;Chinese Acad Sci, Huazhong Normal Univ, Coll Life Sci, Wuhan, Peoples R China.
通讯机构：
[Wang, Hanzhong] C;Chinese Acad Sci, State Key Lab Virol, WIV, Wuhan, Peoples R China.
摘要：
Of 322 stool specimens collected from children with diarrhea from October 2005 through September 2006 in Wuhan, China, group A rotavirus was identified in 101 (31.4%). The most prevalent group A rotavirus genotype was G3P[8] (62.6%), followed by G1P[8](17.6%), G1+G3P[8](8.8%), G3P[4](6.6%), G1P[4](2.2%), and G9P[8](2.2%). The G9 strains were first detected in Wuhan.
作者机构：
[Lei Y.; Ma G.L.; Wang Zh.X.; You D.m.] College;[Lei Y.; Ma G.L.; Wang Zh.X.; You D.m.] of;[Lei Y.; Ma G.L.; Wang Zh.X.; You D.m.] Life;[Lei Y.; Ma G.L.; Wang Zh.X.; You D.m.] Sciences,Central;[Lei Y.; Ma G.L.; Wang Zh.X.; You D.m.] China
