作者：
Du, X. Z.;Zhou, L.;Zhao, H. B.;Wang, Y. F.*;Gui, J. F.
期刊：
Fish Physiology and Biochemistry,2008年34(2):175-184 ISSN：0920-1742
通讯作者：
Wang, Y. F.
作者机构：
[Zhao, H. B.; Du, X. Z.; Wang, Y. F.; Zhou, L.] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Gui, J. F.] Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
通讯机构：
[Wang, Y. F.] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
Hir/Hira (histone regulation) genes were first identified in yeast as negative regulators of histone gene expression. It has been confirmed that HIRA is a conserved family of proteins present in various animals and plants. In this paper, the cDNAs of the Hira homolog named CagHira and CaHira were isolated from gynogenetic gibel carp (gyno-carp) and gonochoristic color crucian carp (gono-carp) respectively. The full-length CagHira is 3,860 bp in length with an open reading frame (ORF) of 3,033 bp that encodes 1,011 amino acids, while the full-length CaHira is 3,748 bp in length and also has an ORF of 3,033 bp. The deduced amino acid sequences of both Hira homologs contain seven WD domains and show high identity with other HIRA family members. RT-PCR analyses revealed strong expression of Hira in the ovaries, whereas no expression was detected in the testes of either of the fishes. Hira transcription was not detected in the liver of gyno-carp, but a high level of Hira mRNA was observed in gono-carp. The temporal expression pattern showed that the Hira mRNA is consistently expressed during all embryonic development stages in gyno-carp. However, the abundance of CaHira mRNA significantly decreased (P < 0.05) shortly after fertilization and then increased again and remained stable from gastrula till hatching. The varying spatiotemporal expression patterns of Hira genes in gyno-carp and gono-carp may be associated with the differing reproductive modes used by these two closely related fishes. Our results suggest that Hira may play a role not only in the decondensation of sperm nucleus and the formation of pronucleus during fertilization, but also in gastrulation and the subsequent development of embryos.
作者机构：
College of Life Science, Central China Normal University, Wuhan 430079, China;Nanyang Technological;Nanyang Technological University, Nanyang Avenue, 639798, Singapore
会议名称：
第十届室内空气品质和质量国际学术会议(Proceedings of the 10th International Conference on Indoor Air Quality and Climate)
会议时间：
2005-09-04
会议地点：
北京
会议论文集名称：
第十届室内空气品质和质量国际学术会议
关键词：
IAQ;Formaldehyde;Blinking frequency;Irritant intensity;Substance P
摘要：
To find out reliable biomarkers in China for acute irritation caused by indoor air pollutants, we carried out this study. A controlled human exposure experiment was used as the main research method. Subject eyes were exposed to gaseous formaldehyde at different doses (0 mg/m3, 1.0 mg/m3, 2.0 mg/m3, 3.0 mg/m3) five minutes a day for 4 days. The content of substance P in nasal lavage was determined by radio immune assay (RIA), and the blinking frequency and irritant intensity of their eyes were measured at the same time. The results showed that there were dose-dependent effects between blinking frequency and formaldehyde levels (r=0.980, P＜0.05), and so were there between irritant intensity and formaldehyde levels (r=0.985, P＜0.05). The content of substance P in nasal lavage increased after exposures, especially after the exposure of3mg/m3 formaldehyde (p＜0.05). This might explain that formaldehyde could stimulate trigeminal nerve ends of the eyes, which would release substance P from its nasal branches through axon reflex.
作者机构：
[Yang, X.; Lu, Z.; Qiao, Y.; Yan, Y.] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Lu, Z.; Li, C. M.; Qiao, Y.; Yan, Y.] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore, Singapore.
通讯机构：
[Yang, X.] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Formaldehyde;Learning;Memory;Morris water maze;NMDA receptor;Oxidative stress
摘要：
UNLABELLED: In this study, we investigated the effect of inhaled formaldehyde on learning and memory capacity. After exposure to 0 (control), 1 and 3 mg/m(3) of gaseous formaldehyde respectively, the behavior of mice in a Morris water maze, the expression of NR1, NR2B mRNA and oxidative damage levels in mice brain were analyzed. The water maze performance, the activities of dismutase superoxide (SOD) and levels of glutathione (GSH) decreased significantly in 3 mg/m(3) group (P < 0.01, compared with control group); while malondialdehyde (MDA) contents and expression of NR1 and NR2B genes increased significantly after exposure to 3 mg/m(3) of gaseous formaldehyde (P < 0.05, <0.01, <0.01, compared with control group). These findings indicate that inhaled formaldehyde negatively affects learning and memory at 3 mg/m(3) of gaseous formaldehyde but not at lower levels. Oxidative stress-induced neuron damages in the brain may be the possible mechanism for these effects. PRACTICAL IMPLICATIONS: This study indicates that inhaled formaldehyde starts to negatively affect learning and memory at a middle concentration of formaldehyde without interference of other indoor air pollutants. Oxidative damage, and the alteration of NMDA receptor expression, which were induced by formaldehyde inhalation, may be the possible mechanism for gaseous formaldehyde-induced neurotoxicity.
期刊：
CHINESE JOURNAL OF PHYSIOLOGY,2008年51(1):13-26 ISSN：0304-4920
通讯作者：
Wu, Fei-Jian
作者机构：
[Wu, Fei-Jian] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Jen, Philip H. -S.] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA.;[Jen, Philip H. -S.] Univ Missouri, Interdisciplinary Neurosci Program, Columbia, MO 65211 USA.
通讯机构：
[Wu, Fei-Jian] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
胰岛是体内重要的内分泌腺,分泌的多种激素参与糖代谢调节.胰岛又称"朗格汉斯岛(Islets of Langerhans)",是德国医生鲍尔*朗格汉斯(Paul Langerhans,1847～1888)在1869年发现的.朗格汉斯在其短暂的一生中在生物学领域做出了诸多贡献,今年是朗格汉斯逝世120周年,本文谨此纪念.
