摘要：
Arabinogalactan proteins (AGPs) are a large family of highly glycosylated of hydroxyproline-rich glycoproteins that play important roles in plant growth, development, and signal transduction. A cDNA encoding a putative classical AGP named GhH6L was isolated from cotton fiber cDNA libraries, and the deduced protein contains 17 copies of repetitive motif of X-Y-proline-proline-proline (where X is serine or alanine and Y is threonine or serine). Northern blotting analysis and quantitative RT-PCR results showed that it was preferentially expressed in 10 days post-anthesis (dpa) fibers and was also developmentally regulated. A promoter fragment was isolated from cotton (Gossypium hirsutum) by genome walking PCR. Expression of beta-glucuronidase (GUS) gene under the GhH6L promoter was examined in the transgenic Arabidopsis plants; only petiole and pedicel were stained, no staining was detected in other tissues. Subcellular localization indicated that GhH6L was localized to the plasma membrane and in the cytoplasm. These data further our understanding of GhH6L as well as shed light on functional insight to GhH6L in cotton.
作者机构：
[Zheng, L. -L.; Cao, W. -B.; Zhang, Z. -F.; Li, X. -B.] Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Cao, W. -B.] Zhengzhou Univ, Basic Med Coll, Zhengzhou 450001, Peoples R China.
通讯机构：
[Cao, W. -B.] Z;Zhengzhou Univ, Basic Med Coll, Zhengzhou 450001, Peoples R China.
摘要：
Analysis of genetic diversity in maize populations is a very important step for understanding genetic structure and subsequently for genetic manipulations in maize breeding. Sh2, Bt2, Sh1, Wx1, Ae1 and Su1 involved in starch biosynthesis are important genes associated with yield and quality traits in maize breeding programs. In this study, genetic diversity of these six genes in 67 Chinese elite maize inbred lines was measured using single-nucleotide amplified polymorphisms (SNAPs). The results indicated that the number of haplotypes of each gene and population was far less than theoretically expected 2n (n = the number of the SNAPs). Phenetic clustering analysis showed that the kernel phonetic (semi-) dent and (semi-) flint lines were belong to distinct subclusters based on haplotypes of SNAPs, with a few exceptions. In addition, the genetic origin of these maize inbred lines was associated with the clustered subgroups. Intragenic linkage disequilibrium (LD) was observed in some of the SNAPs in Bt2, Sh1 and Ae1, while intergenic LD was observed in some of the SNAPs in Bt2, Sh1 and Su1. Association study of kernel phenotypes and SNAP haplotypes showed that the (semi-) dent and (semi-) flint lines had the common haplotype of TA and CC at two SNAP sites in Bt2 (Bt2-2 and Bt2-5), respectively. Two haplotypes of ATGT and GTGC at four SNAP sites in Sh1 (Sh1-2, Sh1-3, Sh1-4 and Sh1-5) were associated with temperature and tropical origin of the maize inbred lines, respectively.
通讯机构：
[Li, Xue-Bao] H;Huazhong Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Cotton (Gossypium hirsutum);Aquaporin;Regulation of gene expression;Root development;Stress
摘要：
Cotton (Gossypium hirsutum), the most important textile crop worldwide, often encounters water stress such as drought or waterlog during its growth season (Summer). To investigate molecular mechanism of water regulation in cotton plants, three cDNAs encoding the plasma membrane intrinsic protein (PIP) were isolated from cotton root cDNA library, and designated GhPIP1;1, GhPIP2;1 and GhPIP2;2, respectively. All of the three PIP proteins displayed water channel activity in Xenopus laevis oocytes. GhPIP2;1 and GhPIP2;2 proteins, however, showed much higher water transport activity than that of the GhPIP1;1 protein. Northern blot analysis revealed that all of the three genes were preferentially expressed in young roots. Further analysis by Real-time quantitative RT-PCR revealed that the transcripts of all the three genes were accumulated at high levels in 3-day-old young roots, but dramatically declined to much lower levels in 6-14 days old roots during seedling development, suggesting that expressions of the isolated GhPIP genes are developmentally regulated in roots. Additionally, expressions of the three genes were remarkably up-regulated or down-regulated under different stresses such as NaCl, cold, PEG (polyethylene glycol) treatments. Collectively, the results suggest that these genes may be involved in root development and in response to stresses.
作者机构：
[李学宝] College of Life Sciences,HuaZhong Normal University
会议名称：
中国遗传学会第八次代表大会暨学术讨论会
会议时间：
2008-10
会议地点：
中国重庆
摘要：
<正>Microsporogenesis,associated with the functional expression of many genes,is a highly programmed and regulated process in flowering plants.To elucidate the roles of the genes during anther developm
作者：
LI Deng-di;WU Ya-jie;RUAN Xiang-mei;QIN Yong-fang;LI Xue-bao
期刊：
棉花学报,2008年(S1):45 ISSN：1002-7807
作者机构：
[LI Deng-di; WU Ya-jie; RUAN Xiang-mei; QIN Yong-fang; LI Xue-bao] College of Life Sciences,Huazhong Normal University,Wuhan 430079,China
会议名称：
2008 ICGI Research Conference(2008年国际棉花基因组研究大会)
会议时间：
2008-7-8
会议地点：
河南安阳
会议主办单位：
中国棉花学会;中国遗传学会;国际棉花基因组学研究学会;中国农业科学院
会议论文集名称：
2008 ICGI Research Conference(2008年国际棉花基因组研究大会)论文集
摘要：
<正>Aquaporins,which form the main water channels in cells,play important roles in plant development.To investigate the functions of aquaporins in cotton fibers,two cDNA(designated GhPIP2;3
摘要：
Homeodomain-leucine zipper （HD-Zip） proteins are transcriptional factors involved in plant development. In this study, one cDNA clone （Gossypium hirsutum homeoboxl, designated GhHB1） encoding HD-Zip protein was isolated from a cotton root cDNA library. The GhHB1 cDNA is 1132 bp in length, including an 828 bp open reading frame that encodes a peptide with 275 amino acids, and 5＇-/3＇- untranslated regions. The predicted GhHB1 protein containing a homeodomaln and a leucine-rich zipper motif shares relatively high identity with other plant HD-Zip proteins. Analysis using quantitative real-time RT-PCR indicated that the GhHB1 gene is predominantly expressed in roots and hypocotyls. Furthermore, GhHB1 transcripts were largely accumulated in early root development, and significantly reduced to very low levels as roots further developed, suggesting that the gene might function in the early development of roots. Under treatment with 1% NaCI, the expression level of the GhHB1 gene was dramatically increased in roots. Likewise, GhHB1 activity in roots was up-regulated by abscisic acid. These results imply that GhHB 1 might play an important role in response to salt stress and to abscisic acid signaling.
摘要：
UDP-Glycosyltransferases (UGT) are a large family of enzymes, which catalyze the transfer of a sugar from an activated sugar donor to an acceptor molecule. Both in plants and in mammals, they are important in the maintenance of cellular homeostasis. In this study, two genes (designated GhUGT1 and GhUGT2, respectively) encoding putative UGT were isolated from the cotton fiber cDNA library. The deduced proteins contain the signature sequences of plant UGTs in the C-terminal region. The GhUGT1 gene encodes a polypeptide of 457 amino acids, and displays homology at amino acid levels with the known glycosyltransferase genes. Sequence analysis revealed that the GhUGT2 merely encodes a small protein, as there is a nucleotide substitution that results in formation of a stop codon in its open reading frame. Real-time RT-PCR analysis revealed that the expression of GhUGT1 is higher in the fast growth tissues, such as in fibers and roots. GhUGT2 has also higher expression in roots, but with lower expression levels in fibers and other tissues. The results also showed that the expression of GhUGT1 is higher than GhUGT2. Further study showed that GhUGT1 and GhUGT2 expressions are regulated under osmotic stress, suggesting they may be involved in plants responding to osmotic stress.
作者机构：
[HUANG Geng-qing; XU Wen-liang; GONG Si-ying; WANG Xiu-lan; LI Xue-bao] College of Life Sciences,Huazhong Normal University,Wuhan 430079,China
会议名称：
2008 ICGI Research Conference(2008年国际棉花基因组研究大会)
会议时间：
2008-7-8
会议地点：
河南安阳
会议主办单位：
中国棉花学会;中国遗传学会;国际棉花基因组学研究学会;中国农业科学院
会议论文集名称：
2008 ICGI Research Conference(2008年国际棉花基因组研究大会)论文集
摘要：
<正>Fasciclin-like arabinogalactan proteins（FLAs）,a subclass of arabinogalactan proteins（AGPs）,are usually involved in cell development in plants.To investigate the expression profiling as well as
通讯机构：
[Li, Xue-Bao] H;Hua Zhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
Fasciclin-like arabinogalactan proteins (FLAs), a subclass of arabinogalactan proteins (AGPs), are usually involved in cell development in plants. To investigate the expression profiling as well as the role of FLA genes in fiber development, 19 GhFLA genes (cDNAs) were isolated from cotton (Gossypium hirsutum). Among them, 15 are predicted to be glycosylphosphatidylinositol anchored to the plasma membranes. The isolated cotton FLAs could be divided into four groups. Real-time quantitative reverse transcriptase polymerase chain reaction results indicated that the GhFLA genes are differentially expressed in cotton tissues. Three genes (GhFLA1/2/4) were specifically or predominantly expressed in 10 days post-anthesis fibers, and the transcripts of the other four genes (GhFLA6/14/15/18) were accumulated at relatively high levels in cotton fibers. Furthermore, expressions of the GhFLA genes are regulated in fiber development and in response to phytohormones and NaCl. The identification of cotton FLAs will facilitate the study of their roles in cotton fiber development and cell wall biogenesis.
