期刊：
BIOTECHNOLOGY AND SUSTAINABLE AGRICULTURE 2006 AND BEYOND,2007年:295-298
通讯作者：
Li, Xue-Bao
作者机构：
[Wang, Xiu-Lan; Wang, Hong; Li, Xue-Bao; Xu, Wen-Liang; Shi, Hai-Yan; Li, Deng-Di] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Li, Xue-Bao] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
14-3-3 protein was originally identified during a systematic classification of bovine brain proteins. Thereafter, a number of 14-3-3 proteins have been found in every eukaryotic organism such as animal, fungi, and plant species. In combination with signal proteins, the 14-3-3s prove to contribute to the regulation of many crucial cellular processes including metabolism, signal transduction, cell-cycle control, apoptosis, protein trafficking, transcription, stress responses, malignant transformation, as well as membrane-transportation of mitochondrion and chloroplast precursor proteins. 14-3-3 proteins are encoded by a multigene family in both animals and plants. Arabidopsis contains 13 functional genes encoding typical 14-3-3 proteins. The 14-3-3 genes have been shown to be expressed in cell or tissue specific manners during Arabidopsis development. Three 14-3-3 genes have been isolated in barley. In potato, up- or down-regulation of the 14-3-3 gene expression altered the composition of lipids, amino acids, and minerals. Furthermore, over-expression of 14-3-3 gene enhances antioxidant activity by 45%, and consequently, delays leaf senescence in potato. Although 14-3-3 genes in a few plant species, such as Arabidopsis, have been well characterized, little is known on cotton (Gossypium hirsutum) 14-3-3 gene, especially its role in regulation of fiber cell elongation. In this study, a cDNA encoding a putative 14-3-3 protein was isolated from fiber cDNA library of cotton. Here, we reported the molecular characterization of the Gh14-3-3L gene and its expression profile in cotton.
期刊：
JOURNAL OF EXPERIMENTAL BOTANY,2007年58(12):3227-3238 ISSN：0022-0957
通讯作者：
Li, Xue-Bao
作者机构：
[Huang, Geng-Qing; Li, Li; Li, Xue-Bao; Wang, Xiu-Lan] Huazhong Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Li, Xue-Bao] H;Huazhong Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
关键词：
Cotton;"expression profiles of GhTUA genes";"fibre elongation";fibre-specific;microtubule;"overexpression in yeast cells"
摘要：
The microtubule cytoskeleton may play an important role in the polarized growth of fibre cells that are single-cell trichomes on the surface of cotton ovules. To investigate whether the high expression levels of α-tubulin genes are correlated with fibre elongation, nine GhTUA genes (cDNAs) encoding α-tubulins with 449–451 amino acid residues were isolated and characterized in cotton. The GhTUA genes share high sequence homology at the nucleotide level (62–93% identity) in the coding region and at the amino acid level (89–99% identity), and can be classified into two subgroups. Real-time quantitative RT-PCR analysis revealed that seven out of the nine GhTUA genes are predominantly expressed in developing fibres. Among them, GhTUA9 displays the highest level of expression, revealing its fibre specificity. The GhTUA9 transcripts in fibres reached its peak value between 5–10 DPA, and dramatically declined to undetectable levels as the ovule matured further, suggesting that its expression is developmentally-regulated in fibres. The GhTUA9 gene including the promoter region was isolated from the cotton genome. To demonstrate the specificity of the GhTUA9 promoter, the 5′-flanking region, including the promoter and 5′-untranslated region, was fused with the GUS gene. Histochemical assays demonstrated that the GhTUA9:GUS gene was specifically expressed in elongating fibres. Overexpression of GhTUA9 in fission yeast (Schizosaccharomyces pombe) promoted atypical longitudinal growth of the host cells by 1.4–1.7-fold, indicating that the GhTUA9 gene is involved in cell elongation. Given all the above results, it is proposed that the GhTUA9 gene may play an important role in fibre elongation.
作者机构：
[Haiyan Shi; Xiulan Wang; Dengdi Li; Wenkai Tang; Hong Wang; Wenliang Xu; Xuebao Li] College of Life Sciences, HuaZhong Normal University, Wuhan 430079, China
通讯机构：
College of Life Sciences, HuaZhong Normal University, China
关键词：
cotton;14-3-3 protein;gene expression
摘要：
The 14-3-3 protein, highly conserved in all eukaryotic cells, is an important regulatory protein. It plays an important role in the growth, amplification, apoptosis, signal transduction, and other crucial life activities of cells. A cDNA encoding a putative 14-3-3 protein was isolated from cotton fiber cDNA library. The cDNA, designated as Gh14-3-3L (Gossypium hirsutum 14-3-3-like),is 1,029 bp in length (including a 762 bp long open reading frame and 5'-/3'-untranslated regions) and deduced a protein with 253 amino acids. The Gh14-3-3L shares higher homology with the known plant 14-3-3 proteins, and possesses the basic structure of 14-3-3 proteins: one dimeric domain, one phosphoralated-serine rich motif, four CC domains, and one EF Hand motif. Northern blotting analysis showed that Gh14-3-3L was predominantly expressed during early fiber development, and reached to the peak of expression in 10 days post anthers (DPA) fiber cells, suggesting that the gene may be involved in regulating fiber elongation. The gene is also expressed at higher level in both ovule and petal, but displays lower or undetectable level of activity in other tissues of cotton.
作者机构：
[Chengwei Zhang; Linlin Guo; Xiulan Wang; Hui Zhang; Haiyan Shi; Wenliang Xu; Xuebao Li] College of Life Science, Central China Normal University, Wuhan 430079, China
通讯机构：
College of Life Science, Central China Normal University, China
关键词：
cotton ADF gene;actin-depolymerizing factor;sequence analysis;molecular evolution;gene different expression
摘要：
Actin depolymerizing factor (ADF), highly conserved in all eukaryotic cells, is a low molecular mass of actin-binding protein, which plays a key role in modulating the polymerizing and depolymerizing of the actin filaments. Four cDNAs (designated GhADF2, GhADF3, GhADF4, and GhADF5, respectively) encoding ADF proteins were isolated from cotton (Gossypium hirsutum) fiber cDNA library. GhADF2 cDNA is 705 bp in length and deduces a protein with 139 amino acids. GhADF3 cDNA is 819 bp in length and encodes a protein of 139 amino acids. GhADF4 cDNA is 804 bp in length and deduces a protein with 143 amino acids. GhADF5 cDNA is 644 bp in length and encodes a protein of 141 amino acids. The molecular evolutionary relationship of these genes was analyzed by means of bioinformatics. GhADF2 is closely related to GhADF3 (99% identity) and PetADF2 (89% iden- tity). GhADF4 is closely related to AtADF6 (78% identity), and GhADF5 is closely related to AtADF5 (83% identity). These results demonstrated that the plant ADF genes are highly conserved in structure. RT-PCR analysis showed that GhADF2 is predominantly expressed in fiber, whereas, GhADF5 is mainly expressed in cotyledons. On the other hand, it seems that GhADF3 and GhADF4 have no tissue specificity. Expression levels of different ADF genes may vary considerably in the same cell type, suggesting that they might be involved in regulating tissue development of cotton and the each ADF isoform may diverge to form the functional difference from the other ADFs during evolution.
