通讯机构：
[Li, DD; Huang, GQ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
Abscisic acid (ABA);Ethylene;GhEIN3;Gossypium hirsutum;Reactive oxygen species (ROS);Salt stress
摘要：
Ethylene insensitive 3 (EIN3), a key transcription factor in ethylene signal transduction, play important roles in plant stress signaling pathways. In this study, we isolated and characterized an EIN3-like gene from cotton (Gossypium hirsutum), designated as GhEIN3. GhEIN3 is highly expressed in vegetative tissues, and its expression is induced by 1-aminocyclopropane-1-carboxylic acid (ACC) and NaCl. Ectopic expression of GhEIN3 in Arabidopsis elevated plants' response to ethylene, which exhibit smaller leaves, more root hairs, shorter roots and hypocotyls. The germination rate, survival rate and root length of GhEIN3 transgenic plants were significantly improved compared to wild type under salt stress. GhEIN3 transgenic plants accumulated less H2O2 and malondialdehyde (MDA), while higher superoxide dismutase (SOD) and peroxidase (POD) activities were detected under salt stress. In addition, expression of several genes related to reactive oxygen species (ROS) pathway and ABA signaling pathway was increased in the GhEIN3 transgenic plants under salt stress. In contrast, virus-induced gene silencing (VIGS) of GhEIN3 in cotton enhanced the sensitivity of transgenic plants to salt stress, accumulating higher H2O2 and MDA and lower SOD and POD activities compared to control plants. Collectively, our results revealed that GhEIN3 might be involved in the regulation of plant response to salt stress by regulating ABA and ROS pathway during plant growth and development.
摘要：
Jasmonate ZIM-domain (JAZ) proteins and DELLA proteins are key negative regulators of jasmonates (JAs) and gibberellin (GA) signaling, respectively. In this study, we found JA and GA synergistically promote fiber cell initiation. We characterized the cellular function of a JAZ protein (GhJAZ3), and a DELLA protein (GhSLR1) of cotton (Gossypium hirsutum). GhJAZ3 is specifically expressed in elongating fibers, while GhSLR1 is expressed in different tissues and at a relatively higher level in 3 DPA ovules. GhSLR1 and GhJAZ3 proteins are localized in the cell nucleus. Yeast two-hybrid analysis indicated that GhSLR1, GhJAZ3 and GhDEL65 could interact with each other, and GhSLR1 could also interact with GhBZR1. Overexpression of GhJAZ3 in Arabidopsis increased hypocotyl and root length, leaf trichome length, and plant height, but decreased the number of leaf trichome, while overexpression of GhSLR1 in Arabidopsis decreased hypocotyl length, leaf trichome length and density. Expression of several leaf trichome initiation determinators (GL3, GL2, TTG2 and MYB23) was down-regulated in GhJAZ3 or GhSLR1 transgenic Arabidopsis, while expression of the cell elongation related genes (EXP1, EXP8, EXPL2 and XTH4) was altered in the GhJAZ3 and GhSLR1 transgenic Arabidopsis. Taken together, these results demonstrate that GhJAZ3 and GhSLR1 function in jasmonate and gibberellin mediated epidermal cell differentiation and elongation.
作者机构：
[Xia, Xiao-Cong; Tao, Miao; Li, Xue-Bao; Yan, Jing-Qiu; Ni, Ping; Huang, Geng-Qing; Li, XB; Li, Wen; Han, Li-Hong] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Huang, GQ; Li, XB] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
摘要：
Plant JAZ (Jasmonate ZIM-domain) proteins play versatile roles in multiple aspects of plant development and defense. However, little is known about the JAZ family in allotetraploid upland cotton (Gossypium hirsutum) so far. In this study, 30 non-redundant JAZ genes were identified in upland cotton through genome-wide screening. Phylogenetic analysis revealed that the 30 proteins in cotton JAZ family are further divided into five groups (I - V), and members in the same group share highly conserved motif structures. Subcellular localization assay demonstrated that GhJAZ proteins are localized in the cell nucleus. Quantitative RT-PCR analysis indicated that GhJAZs display different expression patterns in cotton tissues, and most of them could be induced by Jasmonic (JA). Furthermore, some GhJAZ genes are preferentially expressed in cotton ovules and fibers, and showed differential expression in ovules of wild type cotton and fiberless mutant (fl) during fiber initiation. GhJAZ proteins could interact with each other to form homodimer or heterodimer, and they also interacted with some JA signaling regulators and the proteins involved in cotton fiber initiation. Collectively, our data suggested that some GhJAZ proteins may play important roles in cotton fiber initiation and development by regulating JA signaling as well as some fiber-related proteins.
作者机构：
[Li, Xue-Bao; Huang, Geng-Qing; Tao, Miao; Zhang, Jing-Bo; Hu, Qian-Qian; Li, Deng-Di; Li, Wen; Han, Li-Hong; Wu, Wen-Ying] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Li, XB; Huang, GQ] C;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
摘要：
TCP proteins are plant-specific transcription factors (TFs), and perform a variety of physiological functions in plant growth and development. In this study, 74 non-redundant TCP genes were identified in upland cotton (Gossypium hirsutum L.) genome. Cotton TCP family can be classified into two classes (class I and class II) that can be further divided into 11 types (groups) based on their motif composition. Quantitative RT-PCR analysis indicated that GhTCPs display different expression patterns in cotton tissues. The majority of these genes are preferentially or specifically expressed in cotton leaves, while some GhTCP genes are highly expressed in initiating fibers and/or elongating fibers of cotton. Yeast two-hybrid results indicated that GhTCPs can interact with each other to form homodimers or heterodimers. In addition, GhTCP14a and GhTCP22 can interact with some transcription factors which are involved in fiber development. These results lay solid foundation for further study on the functions of TCP genes during cotton fiber development.
摘要：
Seed oil content is an important agronomic trait in oilseed rape. However, the molecular mechanism of oil accumulation in rapeseeds is unclear so far. In this report, RNA sequencing technique (RNA-Seq) was performed to explore differentially expressed genes in siliques of two Brassica napus lines (HFA and LFA which contain high and low oil contents in seeds, respectively) at 15 and 25 days after pollination (DAP). The RNA-Seq results showed that 65746 and 66033 genes were detected in siliques of low oil content line at 15 and 25 DAP, and 65236 and 65211 genes were detected in siliques of high oil content line at 15 and 25 DAP, respectively. By comparative analysis, the differentially expressed genes (DEGs) were identified in siliques of these lines. The DEGs were involved in multiple pathways, including metabolic pathways, biosynthesis of secondary metabolic, photosynthesis, pyruvate metabolism, fatty metabolism, glycophospholipid metabolism, and DNA binding. Also, DEGs were related to photosynthesis, starch and sugar metabolism, pyruvate metabolism, and lipid metabolism at different developmental stage, resulting in the differential oil accumulation in seeds. Furthermore, RNA-Seq and qRT-PCR data revealed that some transcription factors positively regulate seed oil content. Thus, our data provide the valuable information for further exploring the molecular mechanism of lipid biosynthesis and oil accumulation in B. nupus.
摘要：
PYR/PYL/RCAR proteins are putative abscisic acid (ABA) receptors that play important roles in plant responses to biotic and abiotic stresses. In this study, 27 predicted PYL proteins were identified in cotton (Gossypium hirsutum). Sequence analysis showed they are conserved in structures. Phylogenetic analysis showed that cotton PYL family could be categorized into three groups. Yeast two-hybrid assay revealed that the GhPYL proteins selectively interacted with some GhPP2C proteins. Quantitative RT-PCR analysis indicated that the most of nine GhPYL genes were down-regulated, while the other three were up regulated in cotton under drought stress. Overexpression of GhPYL10/12/26 in Arabidopsis conferred the transgenic plants increased ABA sensitivity during seed germination and early seedling growth. On the contrary, the transgenic seedlings displayed better growth status and longer primary roots under normal conditions and mannitol stress, compared with wild type. Furthermore, the transgenic plants showed the enhanced drought tolerance, relative to wild type, when they were suffered from drought stress. Expression of some stress-related genes in transgenic plants was significant higher than that in wild type under osmotic stress. Thus, our data suggested that these cotton PYL genes may be involved in plant response and defense to drought/osmotic stress. (C) 2017 Elsevier Masson SAS. All rights reserved.
期刊：
Plant Cell, Tissue and Organ Culture,2016年124(3):583-598 ISSN：0167-6857
通讯作者：
Li, Xue-Bao
作者机构：
[Li, Ying; Li, Xue-Bao; Ma, Liu-Feng; Chen, Yun] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Ma, Liu-Feng] Kashgar Univ, Coll Biol & Geog Sci, Xinjiang 844000, Peoples R China.
通讯机构：
[Li, Xue-Bao] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Cotton (Gossypium hirsutum);AP2/ERF protein;Drought and high salinity stress;Abscisic acid (ABA);Overexpression
摘要：
Osmotic stress associated with drought or salinity is a major factor that limits plant growth and productivity. CBF transcription factors play key roles in plant stress signaling transduction pathway. In this work, the data revealed that GhCBF3 identified in cotton (Gossypium hirsutum L.) was remarkably induced by NaCl, mannitol and abscisic acid (ABA). Histochemical assay of GUS activity revealed that GhCBF3 promoter was active in stomata guard cells of the GhCBF3p:GUS transgenic seedlings, and its activity was salt- and osmotic-inducible. Overexpression of GhCBF3 in Arabidopsis resulted in the increased drought- and high salinity-tolerance, but led to an ABA-sensitive phenotype of the transgenic plants. In the presence of NaCl and mannitol, rates of seed germination and cotyledon greening of the GhCBF3 overexpression transgenic plants were higher than those of wild type. Relative water content, proline content and chlorophyll content in the GhCBF3 transgenic seedlings were higher than those in wild type. The GhCBF3 transgenic plants showed greater salt- and drought-tolerance, compared with wild type. In the presence of ABA, stomatal aperture in leaves of the transgenic plants was smaller than that in wild type, and expression levels of AREB1 and AREB2 in the transgenic leaves was remarkably higher than those in wild type. Furthermore, expression of some stress-related genes was altered in the GhCBF3 transgenic plants. These data suggested that GhCBF3 may be involved in regulating stomata closure, thereby enhance plant salt and drought tolerance via ABA signaling pathway.
摘要：
Di19 (drought-induced protein 19) family is a novel type of Cys2/His2 zinc-finger proteins. In this study, we demonstrated that cotton Di19-1 and Di19-2 (GhDi19-1/-2) proteins could be phosphorylated in vitro by the calcium-dependent protein kinase (CDPK). Mutation of Ser to Ala in N-terminus of GhDi19-1/-2 led to the altered subcellular localization of the two proteins, but the constitutively activated form (Ser was mutated to Asp) of GhDi19-1/-2 still showed the nuclear localization. GhDi19-1/-2 overexpression transgenic Arabidopsis seedlings displayed the hypersensitivity to high salinity and abscisic acid (ABA). However, Ser site-mutated GhDi19-1(S116A) and GhDi19-2(S114A), and Ser and Thr double sites-mutated GhDi19-1(S/T-A/A) and GhDi19-2(S/T-A/A) transgenic Arabidopsis did not show the salt- and ABA-hypersensitive phenotypes. In contrast, overexpression of Thr site-mutated GhDi19-1(T114A) and GhDi19-2(T112A) in Arabidopsis still resulted in salt- and ABA-hypersensitivity phenotypes, like GhDi19-1/-2 transgenic lines. Overexpression of GhDi19-1/-2 and their constitutively activated forms in Atcpk11 background could recover the salt- and ABA-insensitive phenotype of the mutant. Thus, our results demonstrated that Ser phosphorylation (not Thr phosphorylation) is crucial for functionally activating GhDi19-1/-2 in response to salt stress and ABA signaling during early plant development, and GhDi19-1/-2 proteins may be downstream targets of CDPKs in ABA signal pathway.
作者机构：
[Zhi-Hao Liu; Yun Chen; Li Feng; Jie Zhang; Deng-Di Li; Xiu-Lan Wang; Xue-Bao Li] Hubei Key Laboratory of Genetic Regulation and Integrative Biology,School of Life Sciences,Central China Normal University,Wuhan 430079,China
会议名称：
湖北省细胞生物学学会2015年会员代表大会暨学术研讨会
会议时间：
2015-10-16
会议地点：
宜昌
会议主办单位：
湖北省细胞生物学学会
会议论文集名称：
湖北省细胞生物学学会2015年会员代表大会暨学术研讨会论文集
关键词：
cotton (Gossypium hirsutum);helix-loop-helix/basic helix-loop-helix (HLH/bHLH) protein;transcription factor;brassinosteroid (BR);fiber development
摘要：
Helix-loop-helix/basic helix-loop-helix(HLH/bHLH)proteins are involved in transcriptional networks controlling a number of biological processes in plants.However,little is known on the roles of HLH/bHLH proteins in cotton(Gossypium hirsutum),especially in fiber development,so far.In this study,we identified a HLH/bHLH gene(designated as GhFP1)in cotton.GhFP1 protein is localized in the cell nucleus,and functions as a transcriptional activator in cells.GhFP1 promotes cotton fiber elongation.Mature fiber length of the transgenic cotton overexpressing GhFP1 was significantly longer than that of wild type.
