摘要：
Background: The negative signaling provided by interactions of the co-inhibitory molecule, programmed death-1 (PD-1), and its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2), is a critical mechanism contributing to tumor evasion; blockade of this pathway has been proven to enhance cytotoxic activity and mediate antitumor therapy. Here we evaluated the anti-tumor efficacy of AAV-mediated delivery of the extracellular domain of murine PD-1 (sPD-1) to a tumor site. Material and Methods: An rAAV vector was constructed in which the expression of sPD-1, a known negative regulator of TCR signals, is driven by human cytomegalovirus immediate early promoter (CMV-P), using a triple plasmid transfection system. Tumor-bearing mice were then treated with the AAV/sPD1 construct and expression of sPD-1 in tumor tissues was determined by semi quantitative RT-PCR, and tumor weights and cytotoxic activity of splenocytes were measured. Results: Analysis of tumor homogenates revealed sPD-1 mRNA to be significantly overexpressed in rAAV/sPD-1 treated mice as compared with control levels. Its use for local gene therapy at the inoculation site of H22 hepatoma cells could inhibit tumor growth, also enhancing lysis of tumor cells by lymphocytes stimulated specifically with an antigen. In addition, PD-1 was also found expressed on the surfaces of activated CD8+ T cells. Conclusion: This study confirmed that expression of the soluble extracellular domain of PD-1 molecule could reduce tumor microenvironment inhibitory effects on T cells and enhance cytotoxicity. This suggests that it might be a potential target for development of therapies to augment T-cell responses in patients with malignancies.
作者机构：
[Yuan Yongze; Zhong Qiu; Guo jia; He Zhen; Hu Xiao-jing; Zhang Kai; Chen Hu-xin; Xiong Li; Liu De-li; Geng Hui] Hubei Key Laboratory of Genetic Regulation and Integrative Biology,College of Life Sciences,Central China Normal University
会议名称：
Environmental Pollution and Public Health（EPPH 2012）
会议时间：
2012-05-17
会议地点：
中国上海
会议论文集名称：
International Conference on Environmental Pollution and Public Health(EPPH 2012) Vol2
摘要：
This study was designed to test the toxic effects of CeO2 in aquatic ecosystems. Scenedesmus obliquus was exposed to a series of CeO2 concentrations (0, 5, 10, 50, 100, 200 mg/L). Five variables were
摘要：
For this study,we adopted 96 h's acute toxicity experiment in order to estimate the toxical effect of the new nano material quantum dot Cadmium Sulfide on Scenedesmus obliquus.It turned out that the stronger inhibited influence on the algae's growth was observed at higher concentration,and the high concentration as 1.0mg/L might lead to marked inhibition.The optical microscopy images illustrated that after the treatment of quantum dot,the cell's shape had changed.And with the increase of concentration of quantum dot Cadmium Sulfide,there was a tendency of pallor of the cells.It indicated that quantum dot Cadmium Sulfide might inhibit the cell's synthesis of chlorophyll.
作者机构：
[Yongze Yuan; Dandan Li; Yongliang Zheng; Pan Wang; Jian Liu; Hui Geng; Li Xiong; Deli Liu] Hubei Key Laboratory of Genetic Regulation and Integrative Biology,College of Life Science,Central China Normal University;[Yongze Yuan; Dandan Li; Yongliang Zheng; Pan Wang; Jian Liu; Hui Geng; Li Xiong; Deli Liu] College of Life Science and Engineering,Huanggang Normal University
会议名称：
Environmental Pollution and Public Health(EPPH 2012)
会议时间：
2012-05-17
会议地点：
中国上海
关键词：
methamidophos;bacterium strains;organophosphorus pesticides;biodegradation;isolation and characterization
摘要：
In the present study, three bacterium strains capable of degrading methamidophos (MAP) were isolated from the soils contaminated with organophosphorus pesticides (OPs). According to morphological char
期刊：
International Journal of Molecular Sciences,2012年13(1):552-557 ISSN：1661-6596
通讯作者：
Wu, Hua
作者机构：
[Wu, Hua; Mu, Taiyang; Li, Xiong; Zhang, Ruiping; Zhao, Mian; Wei, Shichao; Li, Chenliang] Cent China Normal Univ, Coll Life Sci, Anim Behav Res Grp, Wuhan 430079, Peoples R China.;[Wu, Hua] Cent China Normal Univ, Coll Life Sci, Anim Behav Res Grp, 152 Luoyulu, Wuhan 430079, Peoples R China.
通讯机构：
[Wu, Hua] C;Cent China Normal Univ, Coll Life Sci, Anim Behav Res Grp, 152 Luoyulu, Wuhan 430079, Peoples R China.
期刊：
2011 5th International Conference on Bioinformatics and Biomedical Engineering,2011年:1-4
作者机构：
[Kehan Xu; Na Li; Liling Yuan; Yi Liu; Li Xiong; Jiangke Yang; Yunjun Yan; Zhongzhen Li] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, China;[Yongze Yuan; Deli Liu] College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
摘要：
This article has been retracted by the publisher.
作者：
Elhag, Osama A. O.;Hu, Xiaojing;Zhang, Weiying;Xiong, Li;Yuan, Yongze;...
期刊：
2011 5th International Conference on Bioinformatics and Biomedical Engineering,2011年:1-4 ISSN：2151-7614
作者机构：
[Elhag, Osama A. O.] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China
会议名称：
International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2011)
会议时间：
2011-01-01
会议地点：
Wuhan, China
会议论文集名称：
2011 5th International Conference on Bioinformatics and Biomedical Engineering. [v.1]
摘要：
Although T-cell immunoglobulin-and mucin-domain containing molecule 3 (Tim-3) was originally identified as a negatively immunoregulator, the role of Tim-3 in regulating immune response is not fully understand. In this study, we report the finding that intratumor injection of pTim-3, a eukaryotic expression plasmid which expresses murine Tim-3 molecule, potently inhibited tumor growth and results in prolonged survival. These results gain further insight into the function of Tim-3 expressed on nonlymphoid tissues in regulating antitumor immune response. Keywords-antitumor immunity; Tim-3; Ectopic expression
期刊：
2011 5th International Conference on Bioinformatics and Biomedical Engineering,2011年:1-4
作者机构：
[Yongze Yuan; Xueting Yang; Hui Geng; Li Xiong; Yongliang Zheng; Lingfu Deng; Zhongzhen Li] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, China;[Xianfang Wen; Hailing Xi] Research Institute of Chemical Defence, Beijing, China;[Deli Liu] College of Life Science and Engineering, Huanggang Normal University, Huangzhou, China
摘要：
This article has been retracted by the publisher.
作者：
Yan Ni;Pan Wang;Junzhong Yang;Yongze Yuan;Xianfang Wen;...
期刊：
2011 5th International Conference on Bioinformatics and Biomedical Engineering,2011年:1-4
作者机构：
[Yan Ni; Pan Wang; Junzhong Yang; Yongze Yuan; Xianfang Wen; Hailing Xi; Li Xiong; Deli Liu] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan, China;[Wenjing Xiao] Research Institute of Chemical Defence, Beijing, China;[Yongliang Zheng] College of Life Science and Engineering, Huanggang Normal University, Huangzhou, China
摘要：
This article has been retracted by the publisher.
作者：
Can Zhang;Xue Yang;Zhen He;Xiao-jing Hu;Qiu Zhong;...
期刊：
2011 5th International Conference on Bioinformatics and Biomedical Engineering,2011年:1-4
作者机构：
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan, Hubei, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology;[Can Zhang; Xue Yang; Zhen He; Xiao-jing Hu; Qiu Zhong; Jia Guo; Jinlong Wang; Li Xiong; De-li Liu] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan, Hubei, China<&wdkj&>Hubei Key Laboratory of Genetic Regulation and Integrative Biology
摘要：
This article has been retracted by the publisher.
