期刊：
2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010),2010年:1-4 ISSN：2151-7614
通讯作者：
Xiong, Li
作者机构：
[Xiong, Li; He, Zhen; Liu, Deli; Zhou, Qiaoqiao; Mu, Wen; Yang, Xue; Hu, Xiaojing; Zhang, Can] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Xiong, Li] C;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)
会议时间：
2010-06-18
会议地点：
成都
会议主办单位：
[Zhou, Qiaoqiao;Mu, Wen;Yang, Xue;Zhang, Can;Hu, Xiaojing;He, Zhen;Xiong, Li;Liu, Deli] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.
会议论文集名称：
The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)论文集
关键词：
cadmium;uterine and ovary weight;ERa;ER beta;semiquantitative RT-PCR
摘要：
Cadmium, as an inorganic pollutant, its toxic effects on mammals were widely studied, while less attention was paid on its endocrine disrupting effects. In this paper, the effects of CdCl2 on mice uterine-ovary wet weight, modulation of ER (estrogen receptors) mRNA level and their relation were studied. It showed that uterus-ovary wet weight, the expression of ER alpha (alpha subtype) and ER beta (beta subtype) were of significant difference in 930 mu g/kg.bw CdCl2 dosing group compared with the control. However, only up-regulation of ER beta. was detected in 9.3 mu g/kg.bw CdCl2 dosing group. It suggested that the high expression of ER beta might protect mice from increasing of uterus-ovary wet weight. For CdCl2 estrogenic activity, the ER expression mechanism and ER-responsive elements pathway need further examination.
摘要：
Melamine is an organic base and a trimer of cyanamide, with a 1,3,5-triazine skeleton or simply, it's an azocyclo-containing organic compound, and traditionally used in the chemical and sporadically mixed into animal feeds to boost protein content. This study was designed to test the melamine toxicity effects on aquatic ecosystem. S.obliquus was exposed to series melamine concentrations (0, 50, 200, 750 mg.L-1). The growth rate, super oxidase dismutase (SOD), photosynthetic pigments, free malondialdehyde (MDA) contents of S.obliquus were measured after 7d exposure. Melamine concentration of 50, 200mg.L-1 caused a significant increase in growth and photosynthetic pigments contents. The SOD activities of S. obliquus were stimulated first and then inhibited with the increasing of melamine concentrations, and reached the peak in 200mg.L-1 (compared with the control, p < 0.05). The contents of MDA were inhibited significantly under moderate and high concentration (50, 200, 750 mg.L-1) of melamine. This study indicates that, SOD was sensitive to melamine exposure and can be used as a potential biomarker for risk assessment of aquatic ecosystem safety.
摘要：
This study evaluated the effect of butyl benzyl phthalate(BBP), an important class of environmental endocrine disruptors on SOD, AChE, CAT of Danio rerio. Based on the BBP LC50, zebrafish were exposed to different BBP concentrations (0, 0.332, 0.665, 1.33 mg.L-1). The activities were determined in the fish collected at 7d, 14d, 21d and 28d post-exposure. It showed that as the extension of time and the exposure concentrations, from 0d to 28d, the AChE activity was significantly inhibited. The SOD activity increased firstly and then declined with time extension. The CAT activity, in.7d, except 0.665 mg . L-1 group was inhibited significantly (p < 0.01), the rest groups were of no significant difference compared with the control. At 14d, except 0.332 mg . L-1 group, which was always higher than the control, it showed a downward trend after the first rise for other groups; for 28d, the CAT activity of each treated group showed induction effect compared with the control.
摘要：
Methyl parathion hydrolase (MPD) is the key enzyme for methyl parathion degradation. MPD has been widely used to wipe off organophosphorus pesticides. Usually, recombinant MPD was expressed in E.coli BL21 cells predominantly as inclusion bodies with little active enzyme to collect. In this paper, we studied the denaturing of MPD inclusion bodies by urea as well as the protein renatured by diluting and dialyzing. We investigated the effect of urea concentration on the denature efficiency of MPD inclusion bodies;and the certain factors affecting the, efficiency of protein renaturation. These factors are including renature buffer components, pH, GSH/GSSG, and the protein concentration in the renature complex. The results suggested that the optimum urea concentration is 8 M for denature, and the optimum renature condition was 49 g/ml denature proteins diluted in Tris-HCl buffer (pH 8.5) plus 5 mM GSH, 0.5 mM GSSG and 1.5 M urea. Consequently, the renatured MPD reached the electrophoresis purity and its activity was 2.4 U/ml. The specific activity of the MPD reached at 48.8 U/mg, and the renature ratio of the recombinant MPD was 12.5% comparing with the activity of that expressed as soluble proteins.
摘要：
The kinetics and mechanisms of p-nitrophenol (PNP) biodegradation by Pseudomonas aeruginosa HS-D38 were investigated. PNP could be used by HS-D38 strain as the sole carbon, nitrogen and energy source, and PNP was mineralized at the maximum concentration of 500 mg/L within 24 h in an mineral salt medium (MSM). The analytical results indicated that the biodegradation of PNP fit the first order kinetics model. The rate constant KPNP is 2.039×10~(-2) /h in MSM medium, K_(PNP+N) is 3.603×10~(-2) /h with addition of ammonium chloride and K_(PNP+C) is 9.74×10~(-3) /h with additional glucose. Addition of ammonium chloride increased the degradation of PNP. On the contrary, glucose inhibited and delayed the biodegradation of PNP. Chemical analysis results by thin-layer chromatography (TLC), UV-Vis spectroscopy and gas chromatography (GC) techniques suggested that PNP was converted to hydroquinone (HQ) and further degraded via 1,2,4-benzenetriol (1,2,4-BT) pathway.
摘要：
Pseudomonas aeruginosa strain HS-D38 was capable of mineralizing p-nitrophenol (PNP) as the sole source of carbon, nitrogen and energy. Degradation of 200 mg L−1 PNP was examined in different media including: (i) MSM (mineral salts medium, no carbon and nitrogen source); (ii) addition of 1% ammonium chloride as additional nitrogen source (ANM); and (iii) addition of 1% glucose as a carbon source (ACM). Complete degradation of 200 mg L−1 PNP was achieved in 12 h in MSM. Additional ammonium chloride accelerated the PNP degradation, but additional glucose inhibited this process. This strain metabolized as high concentration as 300 and 500 mg L−1 of PNP in 14 h and 24 h, respectively, in MSM. The degradation was accompanied by release of stoichiometric amount of nitrate from PNP. During the bacterial growth on PNP, hydroquinone and 1,2,4-benzenetriol were observed as the key degradation intermediates by using a combination of techniques, including HPLC–DAD and LC–ESI/MS compared with the authentic standards. These results indicated that PNP was degraded via a hydroquinone pathway.
