摘要：
BACKGROUND AND AIMS: Morphological matching between flower and pollinator traits has been documented in diverse plant lineages. Indeed, the matching of corolla-tube length and pollinator-tongue length has been cited repeatedly as a classic case of coevolution. However, there are many possible evolutionary routes to trait matching. Our aim here is both to review the evolutionary mechanisms of plant-pollinator trait matching and to investigate a specific case of trait matching/mismatching in a genus of alpine gingers. METHODS: Roscoea gingers with long corolla tubes in the western Himalayas have pollinators with correspondingly long tongues, but the match between corolla-tube and pollinator-tongue lengths is not seen in the eastern Himalayas. Six floral traits were measured, including corolla-tube depth, an internal trait controlling pollinator access to nectar. We calculated coefficients of variation (CVs) and phylogenetically controlled correlation patterns of these traits in six Roscoea species in order to gain possible insights into stabilizing selection and modularization of these traits. KEY RESULTS: The distal (nectar-containing) portion of the corolla tube exhibited lower CVs that did the basal portion. This is consistent with the hypothesis that pollinators mediate stabilizing selection on the distal, but not basal, portion of the corolla tube. This result, combined with phylogenetic data, suggests that the elevated liquid level of nectar in the distal tube evolved subsequent to dispersal into the eastern Himalayan region and loss of long-tongue pollinators. After accounting for phylogeny, corolla-tube length, anther length, style length, and labellum width were all intercorrelated. Corolla-tube depth was not part of this covariational module, however, suggesting separate adaptation to short-tongued pollinators. CONCLUSIONS: The reduction in functional corolla-tube depth in the Roscoea appears to be related to the loss of long-tongue pollinators associated with dispersal to the eastern Himalayas and pollination by short-tongued pollinators. The apparent mismatch between floral tubes and pollinator tongues is a case of cryptic trait matching between flowers and pollinators, underscoring the importance of combining floral anatomy with pollination ecology, in assessing plant-pollinator trait matching.
期刊：
Food and Chemical Toxicology,2023年173:113642 ISSN：0278-6915
通讯作者：
Mingqing Chen
作者机构：
[Xu, Ke; Chen, Mingqing; Han, Qi; Wang, Xuan; Gao, Xiao; Wei, Zhaolan; Wang, Yunyi; Wang, Shuwei] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Mingqing Chen] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, Hubei, China
摘要：
Time delay and coupling strength are important factors that affect the synchronization of neural networks. In this study, a modular neural network containing subnetworks of different scales was constructed using the Hodgkin-Huxley (HH) neural model; i.e., a small-scale random network was unidirectionally connected to a large-scale small-world network through chemical synapses. Time delays were found to induce multiple synchronization transitions in the network. An increase in coupling strength also promoted synchronization of the network when the time delay was an integer multiple of the firing period of a single neuron. Considering that time delays at different locations in a modular network may have different effects, we explored the influence of time delays within each subnetwork and between two subnetworks on the synchronization of modular networks. We found that when the subnetworks were well synchronized internally, an increase in the time delay within both subnetworks induced multiple synchronization transitions of their own. In addition, the synchronization state of the small-scale network affected the synchronization of the large-scale network. It was surprising to find that an increase in the time delay between the two subnetworks caused the synchronization factor of the modular network to vary periodically, but it had essentially no effect on the synchronization within the receiving subnetwork. By analyzing the phase difference between the two subnetworks, we found that the mechanism of the periodic variation of the synchronization factor of the modular network was the periodic variation of the phase difference. Finally, the generality of the results was demonstrated by investigating modular networks at different scales.
作者机构：
[Yu-tong ZHANG; Yang LIU] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;[Hang-wei LIU] Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China;Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, P.R.China;[Song CAO] Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China<&wdkj&>Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, P.R.China;[Bin LI; Gui-rong WANG] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China<&wdkj&>Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China
摘要：
Spodoptera frugiperda is a highly destructive pest that has become a global problem due to its robust reproductive and migratory capabilities. Transient receptor potential (TRP) channels, which constitute a vast ion channel family, play pivotal roles in sensing the external environment and maintaining internal homeostasis in insects. TRP channels have been widely investigated for their critical roles in regulating various insect behaviors in recent years. In this study, we identified 15 TRP gene loci encoding 26 transcripts in the genome of S. frugiperda and analyzed their expression profiles at different developmental stages. The results revealed that S. frugiperda possesses four TRPC genes, six TRPA genes, one TRPM gene, two TRPV genes, one TRPN gene, and one TRPML gene, while a canonical TRPP is absent. Moreover, the SfruTRPA1 was functionally characterized using the Xenopus oocyte expression system. The results showed that SfruTRPA1 is activated by temperature increases from 20 to 45°C, and there is no significant desensitization after repeated stimuli within the same temperature range. Additionally, SfruTRPA1 is activated by certain natural chemicals, including allyl isothiocyanate (AITC) and cinnamaldehyde (CA). These findings provide valuable insights to the TRP genes in S. frugiperda.
通讯机构：
[Xiong, YZ; Lenhard, M ] U;[Xiong, YZ ] C;Cent China Normal Univ, Sch Life Sci, Luoyu Ave 152, Wuhan 430079, Peoples R China.;Univ Potsdam, Inst Biochem & Biol, Karl Liebknecht Str 24-25, D-14476 Potsdam, Germany.
关键词：
benzaldehyde;β-ocimene;Brassicaceae;Capsella;floral scent;pollinator attraction;quasi-isogenic line
摘要：
PREMISE OF THE STUDY: Floral scent, usually consisting of multiple compounds, is a complex trait and its role in pollinator attraction has received increasing attention. However, it is difficult to disentangle the effect of individual floral scent compounds due to the complexity of isolating the effect of single compounds by traditional methods. METHODS: Using available quasi-isogenic lines (qILs) generated as part of the original mapping of two floral scent volatile-related loci, CNL1 (benzaldehyde) and TPS2 (β-ocimene), in Capsella, we have generated four genotypes that should only systematically differ in these two compounds. Plants of the four genotypes were introduced into a common garden outside the natural range of C. rubella or C. grandiflora, with individuals of a self-compatible C. grandiflora line as pollen donors, whose different genetic background facilitates the detection of outcrossing events. Visitors to flowers of all five genotypes were compared, and the seeds set during the common-garden period were collected for high-throughput amplicon-based sequencing to estimate their outcrossing rates. KEY RESULTS: Benzaldehyde and β-ocimene emissions were detected in the floral scent of corresponding genotypes. While some pollinator groups showed specific visitation preferences depending on scent compounds, the outcrossing rates in seeds did not vary among the four scent-manipulated genotypes. CONCLUSIONS: The successful construction of scent-manipulated Capsella materials using qILs provides a powerful system to study the ecological effects of individual floral scent compounds under largely natural environments. In Capsella, individual BAld and β-ocimene emission may act as attractants for different types of pollinators. This article is protected by copyright. All rights reserved.
摘要：
Brassinosteroids (BRs) play critical roles in plant growth and development and regulate many important agronomic traits. However, the functions of BRs in strawberry are unclear. This study identified two mutants, named P6 and R87, in woodland strawberry (Fragaria vesca) from EMS mutagenesis populations that exhibit narrow leaves, petals and sepals. Mapping by sequencing and genetic studies revealed that the F. vesca CYP734A129, encoding a putative BR catabolic enzyme, is the causative gene for both P6 and R87. Overexpression of CYP734A129 in both F. vesca and Arabidopsis causes a severe dwarf phenotype, and the BRI1-EMS-SUPPRESSOR 1 (BES1) protein is less abundant in the CYP734A129-overexpressing Arabidopsis seedlings. This suggests that CYP734A129 is functionally conserved with CYP734A1, as a BR-inactivating enzyme. Transcriptome analysis of young leaves revealed that four BR biosynthetic genes were significantly downregulated in P6 (cyp734a129), and photosynthesis-related genes were highly enriched among the up-regulated genes in P6 compared to the wild type. This further supports that CYP734A129 inactivates BRs in F. vesca. Furthermore, we showed that mutations in CYP734A129 do not affect fruit shape and color during ripening in strawberry. Overall, our results suggest that F. vesca CYP734A129 is a BR catabolic enzyme, and provide insights into the roles of CYP734A129 in strawberry.
作者机构：
[Fang, Yiwei; Peng, Meilin; Zhao, Kai; Liu, Chunyan] Huazhong Univ Sci & Technol, Inst Reprod Hlth, Tongji Med Coll, Wuhan 430030, Peoples R China.;[Zhang, Zheng; Yi, Guilin] Wuhan Prevent & Treatment Ctr Occupat Dis, Wuhan 430015, Peoples R China.;[Zhang, Zheng] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Zhao, Yun; Sun, Guoqiang; Yang, Hui] Huazhong Univ Sci & Technol, Maternal & Child Hlth Hosp Hubei Prov, Tongji Med Coll, Dept Obstet, Wuhan 430070, Peoples R China.;[Zhao, Kai] Henan Inst Reprod Hlth Sci & Technol, NHC Key Lab Birth Defects Prevent, Zhengzhou 450000, Peoples R China.
通讯机构：
[Guilin Yi] W;[Kai Zhao] I;[Hui Yang] D;Department of Obstetrics, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430070, China<&wdkj&>Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China<&wdkj&>NHC Key Laboratory of Birth Defects Prevention, Henan Institute of Reproduction Health Science and Technology, Zhengzhou, 450000, China<&wdkj&>Wuhan Prevention and Treatment Center for Occupational Diseases, Wuhan, 430015, China
作者机构：
[Huang, Rong; Deng, Youchao; Iyamu, Iredia D.] Purdue Univ, Inst Drug Discovery, Ctr Canc Res, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA.;[Dong, Aiping; Min, Jinrong; Song, Xiaosheng] Univ Toronto, Dept Physiol, Struct Genom Consortium, Toronto, ON M5G 1L7, Canada.;[Dong, Aiping; Min, Jinrong; Song, Xiaosheng] Univ Toronto, Dept Physiol, Toronto, ON M5G 1L7, Canada.;[Min, Jinrong] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Deng, Youchao] Cent South Univ, Xiangya Sch Pharmaceut Sci, Changsha 410013, Peoples R China.
通讯机构：
[Huang, R ] P;[Min, JR ] U;Purdue Univ, Inst Drug Discovery, Ctr Canc Res, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA.;Univ Toronto, Dept Physiol, Struct Genom Consortium, Toronto, ON M5G 1L7, Canada.;Univ Toronto, Dept Physiol, Toronto, ON M5G 1L7, Canada.
摘要：
Protein arginine methyltransferases (PRMTs) are attractive targets for developing therapeutic agents, but selective PRMT inhibitors targeting the cofactor SAM binding site are limited. Herein, we report the discovery of a noncanonical but less polar SAH surrogate YD1113 by replacing the benzyl guanidine of a pan-PRMT inhibitor with a benzyl urea, potently and selectively inhibiting PRMT3/4/5. Importantly, crystal structures reveal that the benzyl urea moiety of YD1113 induces a unique and novel hydrophobic binding pocket in PRMT3/4, providing a structural basis for the selectivity. In addition, YD1113 can be modified by introducing a substrate mimic to form a "T-shaped" bisubstrate analogue YD1290 to engage both the SAM and substrate binding pockets, exhibiting potent and selective inhibition to type I PRMTs (IC50 < 5 nmol/L). In summary, we demonstrated the promise of YD1113 as a general SAH mimic to build potent and selective PRMT inhibitors.(c) 2023 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
通讯作者：
Hong Yang<&wdkj&>Hong Yang Hong Yang Hong Yang
作者机构：
[Yang, Hong; Li, Xin-Yu; Mei, Cheng; Wulamu, Dilinuer] Cent China Normal Univ, Inst Entomol, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhan, Shuai; Luo, Xing-Yu; Huang, Yong-Ping] Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, CAS Key Lab Insect Dev & Evolutionary Biol, Inst Plant Physiol & Ecol,Shanghai Inst Biol Sci, Shanghai, Peoples R China.
通讯机构：
[Hong Yang; Hong Yang Hong Yang Hong Yang] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Institute of Entomology, School of Life Sciences, Central China Normal University, Wuhan, China
关键词：
bacterial isolates;germ-free BSF larvae;growth and development;Hermetia illucens;promotive effects
摘要：
Graphical Abstract Analysis of the intestinal bacterial community composition of Hermetia illucens (black soldier fly, BSF) larvae (BSFL) with pyrosequencing showed that the dynamics of bacterial community composition among different larval instars were striking at the genus level. Klebsiella, Clostridium, Providencia, Dysgonomonas, Dysgonomonas, and Providencia were the relatively most abundant bacterial groups in the 1st‐ to 6th‐instar BSFL, respectively. Providencia, Citrobacter, Klebsiella, Ochrobactrum, and Dysgonomonas promoted the growth and development of BSF significantly compared with the germ‐free control by increasing the weight gain of larvae and pupae, as well as the prepupae rate and eclosion rate. In addition, Citrobacter, Klebsiella, and Providencia could shorten the life cycle of BSF significantly. Abstract Black soldier fly (BSF), Hermetia illucens (Diptera: Stratiomyidae), is a prominent insect for the bioconversion of various organic wastes. As a saprotrophic insect, the BSF inhabits microbe‐rich environments. However, the influences of the intestinal microorganisms on BSF growth and development are not very clear. In this study, the dynamics of the intestinal bacterial community of BSF larvae (BSFL) were analyzed using pyrosequencing. Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria were the most prevalent bacterial phyla in the intestines of all larval instars. The dynamic changes in bacterial community compositions among different larval instars were striking at the genus level. Klebsiella, Clostridium, Providencia, and Dysgonomonas were the relatively most abundant bacteria in the 1st‐ to 4th‐instar BSFL, respectively. Dysgonomonas and Providencia also dominated the 5th‐ and 6th‐instar larvae, at ratios of 31.1% and 47.2%, respectively. In total, 148 bacterial strains affiliated with 20 genera were isolated on different media under aerobic and anaerobic conditions. Among them, 6 bacteria, BSF1–BSF6, were selected for further study. The inoculation of the 6 isolates independently into germ‐free BSFL feeding on an artificial diet showed that all the bacteria, except BSF4, significantly promoted BSF growth and development compared with the germ‐free control. Citrobacter, Dysgonomonas, Klebsiella, Ochrobactrum, and Providencia promoted BSF development significantly by increasing the weight gains of larvae and pupae, as well as increasing the prepupae and eclosion rates. In addition, Citrobacter, Klebsiella and Providencia shortened the BSF life cycle significantly. The results illustrate the promotive effects of intestinal bacteria on BSF growth and development.
期刊：
American Journal of Botany,2023年110(6) ISSN：0002-9122
通讯作者：
Huang, SQ
作者机构：
[Huang, Shuang-Quan; Huang, SQ; Hao, Kai] Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.;[Hao, Kai] Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable Ut, South China Bot Garden, Guangzhou 510650, Peoples R China.;[Fang, Qiang] Henan Univ Sci & Technol, Coll Agr, Luoyang 471003, Peoples R China.
通讯机构：
[Huang, SQ ] C;Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.
关键词：
Caryophyllaceae;conspecific pollen limitation;generalized flower;heterospecific pollen deposition;pollen fitness;pollen species diversity;seed set;Silene;tolerance and avoidance strategy
摘要：
Abstract Premise Co‐flowering species that have not evolved an avoidance mechanism may have tolerance to heterospecific pollen (HP) deposition as an adaptive strategy to minimize any deleterious effects of HP transfer, but empirical evidence for the tolerance hypothesis remains scarce. Methods To estimate the potential effects of heterospecific pollen deposition (HPD) on female reproductive success, we counted conspecific (CP) and HP pollen grains deposited on stigmas and assessed subsequent seed set of both open‐ and hand‐pollinated flowers in three co‐flowering Silene species with exposed stigmas that usually received numerous HP grains on the elongated receptive area. Results The percentage of HP grains per flower (HP%) varied from 16.6% to 43.0% among three species. Silene chungtienensis had lower HP%, and the CP‐HP relationship was neutral; S. gracilicaulis and S. yunnanensis had a relatively higher HP% with a positive CP‐HP relationship. The effects of CP and HP number on natural seed set were positive for all three species, but HP% had stronger negative effects in S. chungtienensis and S. gracilicaulis. In hand‐pollinated flowers of the three Silene species, seed set did not decrease with HP whether CP was in excess or insufficient, indicating no negative effects of HPD on seed production. Conclusions Consistent with the tolerance hypothesis, our results indicated that species with higher HP interference are likely to be tolerant to an increase in HP%. These species with generalist‐pollinated flowers and exposed large stigmas may benefit from an increase of conspecific pollen deposition, despite the associated increase in heterospecific pollen deposition.
作者机构：
Faculty of Resources and Environmental Science, Hubei University, Wuhan 430062, China;[Shuang-Quan Huang; Ze-Yu Tong] Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, China;[Ling-Yun Wu] Faculty of Resources and Environmental Science, Hubei University, Wuhan 430062, China<&wdkj&>Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, China
通讯机构：
[Shuang-Quan Huang; Ze-Yu Tong] I;Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, China
摘要：
Reproductive strategies of sexually dimorphic plants vary in response to the environment. Here, we ask whether the sexual systems of Fagopyrum species (i.e., selfing homostylous and out-crossing distylous) represent distinct adaptive strategies to increase reproductive success in changing alpine environments. To answer this question, we determined how spatial and temporal factors (e.g., elevation and peak flowering time) affect reproductive success (i.e., stigmatic pollen load) in nine wild Fagopyrum species (seven distylous and two homostylous) among 28 populations along an elevation gradient of 1299–3315 m in the Hengduan Mountains, southwestern China. We also observed pollinators and conducted hundreds of hand pollinations to investigate inter/intra-morph compatibility, self-compatibility and pollen limitation in four Fagopyrum species (two distylous and two homostylous). We found that Fagopyrum species at higher elevation generally had bigger flowers and more stigmatic pollen loads; late-flowering individuals had smaller flowers and lower pollen deposition. Stigmatic pollen deposition was more variable in distylous species than in homostylous species. Although seed set was not pollen-limited in all species, we found that fruit set was much lower in distylous species, which rely on frequent pollinator visits, than in homostylous species capable of autonomous self-pollination. Our findings that pollination success increases at high elevations and decreases during the flowering season suggest that distylous and homostylous species have spatially and temporally distinct reproductive strategies related to environment-dependent pollinator activity.
通讯作者：
Chuan YAN<&wdkj&>Chuan YAN Chuan YAN Chuan YAN
作者机构：
College of Ecology and Environmental Science, Yunnan University, Kunming, China;Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology and Institute of Biodiversity, Yunnan University, Kunming, China;[Hongmao ZHANG; Hongmao ZHANG Hongmao ZHANG Hongmao ZHANG] Institute of Evolution and Ecology, College of Life Sciences, Central China Normal University, Wuhan, China;[Rui LIU; Yongjun ZHANG; Chuan YAN; Rui LIU Rui LIU Rui LIU; Yongjun ZHANG Yongjun ZHANG Yongjun ZHANG; Chuan YAN Chuan YAN Chuan YAN] State Key Laboratory of Grassland Agro-ecosystems, College of Ecology & School of Life Sciences, Lanzhou University, Lanzhou, China;[Lin CAO; Lin CAO Lin CAO Lin CAO] College of Ecology and Environmental Science, Yunnan University, Kunming, China<&wdkj&>Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology and Institute of Biodiversity, Yunnan University, Kunming, China
通讯机构：
[Chuan YAN; Chuan YAN Chuan YAN Chuan YAN] S;State Key Laboratory of Grassland Agro-ecosystems, College of Ecology & School of Life Sciences, Lanzhou University, Lanzhou, China
关键词：
density dependence;mast;resource pulse;rodent;seed
摘要：
Our study provides a global pattern of the associations between seed falls and rodent population dynamics in forests, and density dependence contributed much more to rodent dynamics than seed falls. Abstract One classic system of pulsed resource and animal population is mast seeding and population dynamics of seed‐eating rodents in forests. However, we still lack an understanding of the global patterns regarding the contributions of seed falls to rodent outbreaks or population dynamics. We analyzed a global dataset of coupled long‐term time series of seed abundances and rodent populations from published literature, including 66 and 89 time series (156 rodent–seed pairs from 37 studies) for rodent and seed abundances, respectively. We found only half of the examined rodent populations showed statistically significant coincidence between rodent outbreak and mast‐seeding years. Over all the coupled time series, seed abundance was found to positively correlate with rodent abundance with a one‐year lag, and the relative importance of seed abundance was much lower than that of density dependence in affecting rodent population growth rates. We also found the relative importance of seed abundance decreased, but that of rodent density dependence increased with the latitude of study. For the first time, our work provides a global pattern on the associations between seed falls and rodent population dynamics mostly in mid‐ and high‐latitude forests, and highlights the necessity of more long‐term studies on this subject in more forest ecosystems.
期刊：
Computational and Structural Biotechnology Journal,2023年21:168-175 ISSN：2001-0370
通讯作者：
Xiongfeng Dai<&wdkj&>Manlu Zhu
作者机构：
[Han, Fei; Dai, Xiongfeng; Wang, Yanling; Zhu, Manlu; Wang, Qian; Mu, Haoyan] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.
通讯机构：
[Xiongfeng Dai; Manlu Zhu] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, Hubei Province, China<&wdkj&>Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, Hubei Province, China
关键词：
(p)ppGpp;Growth control;Resource allocation;Bacteria;Plant and green algaes
摘要：
Rapid growth and survival are two key traits that enable bacterial cells to thrive in their natural habitat. The guanosine tetraphosphate and pentaphosphate [(p)ppGpp], also known as "magic spot", is a key sec-ond messenger inside bacterial cells as well as chloroplasts of plants and green algae. (p)ppGpp not only controls various stages of central dogma processes (replication, transcription, ribosome maturation and translation) and central metabolism but also regulates various physiological processes such as pathogen-esis, persistence, motility and competence. Under extreme conditions such as nutrient starvation, (p) ppGpp-mediated stringent response is crucial for the survival of bacterial cells. This mini-review high-lights some of the very recent progress on the key role of (p)ppGpp in bacterial growth control in light of cellular resource allocation and cell size regulation. We also briefly discuss some recent functional insights into the role of (p)ppGpp in plants and green algae from the angle of growth and development and further discuss several important open directions for future studies.(c) 2022 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open access article under the CC BY-NC-ND license (http://creative-commons.org/licenses/by-nc-nd/4.0/).
作者：
Ye, Zhiqiang;Pfrender, Michael E.;Lynch, Michael
期刊：
Genome Biology and Evolution,2023年15(11) ISSN：1759-6653
通讯作者：
Ye, ZQ
作者机构：
[Ye, Zhiqiang] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.;[Pfrender, Michael E.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA.;[Lynch, Michael] Arizona State Univ, Biodesign Ctr Mech Evolut, Tempe, AZ USA.
通讯机构：
[Ye, ZQ ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.
关键词：
Daphnia;hybridization;linkage disequilibrium;local adaptation;population genomics;recombination;sister species
摘要：
Studies of closely related species with known ecological differences provide exceptional opportunities for understanding the genetic mechanisms of evolution. In this study, we compared population-genomics data between Daphnia pulex and Daphnia pulicaria, two reproductively compatible sister species experiencing ecological speciation, the first largely confined to intermittent ponds and the second to permanent lakes in the same geographic region. Daphnia pulicaria has lower genome-wide nucleotide diversity, a smaller effective population size, a higher incidence of private alleles, and a substantially more linkage disequilibrium than D. pulex. Positively selected genes in D. pulicaria are enriched in potentially aging-related categories such as cellular homeostasis, which may explain the extended life span in D. pulicaria. We also found that opsin-related genes, which may mediate photoperiodic responses, are under different selection pressures in these two species. Genes involved in mitochondrial functions, ribosomes, and responses to environmental stimuli are found to be under positive selection in both species. Additionally, we found that the two species have similar average evolutionary rates at the DNA-sequence level, although approximately 160 genes have significantly different rates in the two lineages. Our results provide insights into the physiological traits that differ within this regionally sympatric sister-species pair that occupies unique microhabitats.
摘要：
Understanding trophic niche differentiation is critical for exploring interspecific competition and stable coexistence among morphologically similar sympatric species. Bats are an ideal model for studying trophic niche differentiation among species because of their high taxonomic and ecological diversities, as well as their special life history traits. Although many factors can affect bat trophic niches, few studies have combined multiple factors to investigate the influences on bat trophic niches. In this study, we analyzed the summer diet and potential influencing factors of five sympatric rhinolophid bats in southwestern China: Rhinolophus macrotis, Rhinolophus osgoodi, Rhinolophus ferrumequinum, Rhinolophus affinis, and Rhinolophus pusillus. All five species mainly fed on Lepidopteran and Dipteran insects, with a large trophic niche breadth for each species and a low degree of dietary overlap. With the exception of R. affinis and R. pusillus, significant differences in diet composition were detected among species, which indicated relatively low interspecific competition. Canonical correspondence analysis showed that both echolocation calls and body size significantly affected interspecific diet differentiation, while wing morphology and bite force had relatively weak effects. This study suggests that the combined effects of multiple factors may drive trophic niche differentiation among five rhinolophid bat species in the study area.
期刊：
JOURNAL OF PROTEOME RESEARCH,2023年22(9):2814-2826 ISSN：1535-3893
通讯作者：
Wan, CH
作者机构：
[Wan, Cuihong; Wan, CH; Ding, Yuhe; Gu, Jingyu; Peng, Zhao; Yan, Jiahao; Qin, Lu] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Wan, Cuihong; Wan, CH; Ding, Yuhe; Gu, Jingyu; Peng, Zhao; Yan, Jiahao; Qin, Lu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Wan, CH ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
zebrafish;early life stage;development;maternal-to-zygotic transition;mass spectrometry
摘要：
The early development of zebrafish (Danio rerio) is a complex and dynamic physiological process involving cell division, differentiation, and movement. Currently, the genome and transcriptome techniques have been widely used to study the embryonic development of zebrafish. However, the research of proteomics based on proteins that directly execute functions is relatively vacant. In this work, we apply label-free quantitative proteomics to explore protein profiling during zebrafish's embryogenesis, and a total of 5961 proteins were identified at 10 stages of zebrafish's early development. The identified proteins were divided into 11 modules according to weighted gene coexpression network analysis (WGCNA), and the characteristics between modules were significantly different. For example, mitochondria-related functions enriched the early development of zebrafish. Primordial germ cell-related proteins were identified at the 4-cell stage, while the eye development event is dominated at 5 days post fertilization (dpf). By combining with published transcriptomics data, we discovered some proteins that may be involved in activating zygotic genes. Meanwhile, 137 novel proteins were identified. This study comprehensively analyzed the dynamic processes in the embryonic development of zebrafish from the perspective of proteomics. It provided solid data support for further understanding of the molecular mechanism of its development.
