作者机构：
[Huang, Rong; Deng, Youchao; Iyamu, Iredia D.] Purdue Univ, Inst Drug Discovery, Ctr Canc Res, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA.;[Dong, Aiping; Min, Jinrong; Song, Xiaosheng] Univ Toronto, Dept Physiol, Struct Genom Consortium, Toronto, ON M5G 1L7, Canada.;[Dong, Aiping; Min, Jinrong; Song, Xiaosheng] Univ Toronto, Dept Physiol, Toronto, ON M5G 1L7, Canada.;[Min, Jinrong] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Deng, Youchao] Cent South Univ, Xiangya Sch Pharmaceut Sci, Changsha 410013, Peoples R China.
通讯机构：
[Huang, R ] P;[Min, JR ] U;Purdue Univ, Inst Drug Discovery, Ctr Canc Res, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA.;Univ Toronto, Dept Physiol, Struct Genom Consortium, Toronto, ON M5G 1L7, Canada.;Univ Toronto, Dept Physiol, Toronto, ON M5G 1L7, Canada.
摘要：
Protein arginine methyltransferases (PRMTs) are attractive targets for developing therapeutic agents, but selective PRMT inhibitors targeting the cofactor SAM binding site are limited. Herein, we report the discovery of a noncanonical but less polar SAH surrogate YD1113 by replacing the benzyl guanidine of a pan-PRMT inhibitor with a benzyl urea, potently and selectively inhibiting PRMT3/4/5. Importantly, crystal structures reveal that the benzyl urea moiety of YD1113 induces a unique and novel hydrophobic binding pocket in PRMT3/4, providing a structural basis for the selectivity. In addition, YD1113 can be modified by introducing a substrate mimic to form a "T-shaped" bisubstrate analogue YD1290 to engage both the SAM and substrate binding pockets, exhibiting potent and selective inhibition to type I PRMTs (IC50 < 5 nmol/L). In summary, we demonstrated the promise of YD1113 as a general SAH mimic to build potent and selective PRMT inhibitors.(c) 2023 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
作者：
Ye, Zhiqiang;Pfrender, Michael E.;Lynch, Michael
期刊：
Genome Biology and Evolution,2023年15(11) ISSN：1759-6653
通讯作者：
Ye, ZQ
作者机构：
[Ye, Zhiqiang] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.;[Pfrender, Michael E.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA.;[Lynch, Michael] Arizona State Univ, Biodesign Ctr Mech Evolut, Tempe, AZ USA.
通讯机构：
[Ye, ZQ ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.
关键词：
Daphnia;hybridization;linkage disequilibrium;local adaptation;population genomics;recombination;sister species
摘要：
Studies of closely related species with known ecological differences provide exceptional opportunities for understanding the genetic mechanisms of evolution. In this study, we compared population-genomics data between Daphnia pulex and Daphnia pulicaria, two reproductively compatible sister species experiencing ecological speciation, the first largely confined to intermittent ponds and the second to permanent lakes in the same geographic region. Daphnia pulicaria has lower genome-wide nucleotide diversity, a smaller effective population size, a higher incidence of private alleles, and a substantially more linkage disequilibrium than D. pulex. Positively selected genes in D. pulicaria are enriched in potentially aging-related categories such as cellular homeostasis, which may explain the extended life span in D. pulicaria. We also found that opsin-related genes, which may mediate photoperiodic responses, are under different selection pressures in these two species. Genes involved in mitochondrial functions, ribosomes, and responses to environmental stimuli are found to be under positive selection in both species. Additionally, we found that the two species have similar average evolutionary rates at the DNA-sequence level, although approximately 160 genes have significantly different rates in the two lineages. Our results provide insights into the physiological traits that differ within this regionally sympatric sister-species pair that occupies unique microhabitats.
期刊：
Physiological and Molecular Plant Pathology,2023年125:102002 ISSN：0885-5765
通讯作者：
Za Khai Tuang
作者机构：
[Tuang, Za Khai] Cent China Normal Univ, Sch Life Sci, Wuhan, Peoples R China.;[Tuang, Za Khai] Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, Dept Plant Pathol & Microbiol, Rehovot, Israel.;[Ling, Tial C.] Chiang Mai Univ, Fac Sci, Dept Biol, Bee Protect Lab, Chiang Mai 50200, Thailand.
通讯机构：
[Za Khai Tuang] S;School of Life Sciences, Central China Normal University, Wuhan, China<&wdkj&>Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
摘要：
The adaptation process is a way of life for plants, and they must contend with biotic and abiotic stresses for their chance of survival. These stresses can lead to disastrous crop production and limited distribution of crops geographically. The use of chemicals to address those stresses in agriculture is limited, while management strategies often fail to meet the needs of the industry. Therefore, novel techniques are needed to control plant diseases and to maintain food security. RCF1 has been found to encode an RNA helicase containing DEAD-box domains and loss-of-function mutation of this gene increases the sensitivity of plants to cold stress. In this study, a single mutant generated in RCF1 was used to investigate the function of the RCF1 gene in pathogen infection stress (i.e., Pst DC3000 and B. cinerea). RFC1 was found to confer resistance to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) but negatively affect Botrytis cinerea (B. cinerea) infection by repressing SNI1 pathways and JA signaling while activating SA pathways and its receptor NPR1. The generated rcf1 allelic mutant (name: 407) showed sensitivities to bacteria pathogen (Pst DC3000) but tolerance to fungus infection (B. cinerea), and SNI1 was highly upregulated in it. Free and conjugated Salicylic Acid (SA) contents were much lower in the allelic mutant 407 than in complementary materials and is sensitive to Pst DC3000 infection, and NPR1 depending on SA biosynthetic genes were down-regulated in it. Our preliminary results reveal that RCF1 confers resistance to Pst DC3000 by SA and NPR1 dependence manner but blocks SNI1 and JA pathways.
摘要：
The field of genomics has ushered in new methods for studying molecular-genetic variation in natural populations. However, most population-genomic studies still rely on small sample sizes (typically, <100 individuals) from single time points, leaving considerable uncertainties with respect to the behavior of relatively young (and rare) alleles and, owing to the large sampling variance of measures of variation, to the specific gene targets of unusually strong selection. Genomic sequences of ∼1,700 haplotypes distributed over a 10-year period from a natural population of the microcrustacean Daphnia pulex reveal evolutionary-genomic features at a refined scale, including previously hidden information on the behavior of rare alleles predicted by recent theory. Background selection, resulting from the recurrent introduction of deleterious alleles, appears to strongly influence the dynamics of neutral alleles, inducing indirect negative selection on rare variants and positive selection on common variants. Temporally fluctuating selection increases the persistence of nonsynonymous alleles with intermediate frequencies, while reducing standing levels of variation at linked silent sites. Combined with the results from an equally large metapopulation survey of the study species, classes of genes that are under strong positive selection can now be confidently identified in this key model organism. Most notable among rapidly evolving Daphnia genes are those associated with ribosomes, mitochondrial functions, sensory systems, and lifespan determination.
期刊：
Computational Biology and Bioinformatics,2023年11(1):13-18 ISSN：2330-8265
作者机构：
1College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, People’s Republic of China;2Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, People’s Republic of China
关键词：
Keywords Alternative Splicing;Intron Retention;Cis-elements;Rice;Drought Stress
摘要：
Pre-mRNAs splicing is one of the fundamental process which generates multiple transcripts from a single gene, contributing to transcriptome and proteome diversity. AS is regulated by the cooperation of trans-factors and cis-elements. In plants, extensive alternative splicing occurs not only in tissue-specific manner but also in response to stress conditions. Intron retention is the most predominant splicing type. However, the cis-elements regulating intron retention are still ambiguous in plants, especially under environmental stresses. This study aimed to elucidate the cis-elements underlying intron retention in plants under adverse enrironments. Using RNA-seq data of rice cultivars IRAT109 and ZS97 under drought environments, we compared the sequence characteristics between constitutive and retained introns. The results show that the main AS types include intron retention (IR), alternative acceptor sites (AA), alternative donor sites (AD) and cassette exon (exon skipping, ES). Among of them, IR was the prevelent pattern with frequencies of 30.8-31.2%. Motif analysis of 5' and 3' 200bp intron sequences found rich U(T) in the motifs for both constitutive and retained introns. By further analysis of base composition of sequences flanking splice sites, we detected a notable difference in U(T) content between introns and their neighboring exons in constitutive introns, but not in retained introns. The results in this study suggested that the lack of significant changes in U(T) content between retained introns and neighboring exons might be a potential cis feature of intron retention.
作者机构：
[Mao, Bin; Chen, Meng-Yan; Wang, Qian; Shen, Wei; Li, Chao; Wang, Yu-Feng; Zheng, Ya; Duan, Xin] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, YF ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Ocnus;Drosophila melanogaster;Proteomics;Phosphoproteomics;Testis development
摘要：
Testis is the only organ supporting sperm production and with the largest number of proteins and tissue-specific proteins in animals. In our previous studies, we have found that knockdown of ocnus (ocn), a testis-specific gene, resulted in much smaller testis with no germ cells in Drosophila melanogaster. However, the molecular consequences of ocn knockdown in fly testes are unknown. In this study, through iTRAQ quantitative proteomics sequencing, 606 proteins were identified from fly abdomens as having a significant and at least a 1.5-fold change in expression after ocn knockdown in fly testes, of which 85 were up-regulated and 521 were down-regulated. Among the differential expressed proteins (DEPs), apart from those proteins involved in spermatogenesis, the others extensively affected biological processes of generation of precursor metabolites and energy, metabolic process, and mitochondrial transport. Protein-protein interaction (PPI) analyses of DEPs showed that several kinases and/or phosphatases interacted with Ocn. Re-analyses of the transcriptome revealed 150 differential expressed genes (DEGs) appeared in the DEPs, and their changing trends in expressions after ocn knockdown were consistent. Many common down-regulated DEGs and DEPs were testis-specific or highly expressed in the testis of D. melanogaster. Quantitative RT-PCR (qRT-PCR) confirmed 12 genes appeared in both DEGs and DEPs were significantly down-regulated after ocn knockdown in fly testes. Furthermore, 153 differentially expressed phosphoproteins (DEPPs), including 72 up-regulated and 94 down-regulated phosphorylated proteins were also identified (13 phosphoproteins appeared in both up- and down-regulated groups due to having multiple phosphorylation sites). In addition to those DEPPs associated with spermatogenesis, the other DEPPs were enriched in actin filament-based process, protein folding, and mesoderm development. Some DEPs and DEPPs were involved in Notch, JAK/STAT, and cell death pathways. Given the drastic effect of the ocn knockdown on tissue development and testis cells composition, the differences in protein abundance in the ocn knockdown flies might not necessarily be the direct result of differential gene regulation due to the inactivation of ocn. Nevertheless, our results suggest that the expression of ocn is essential for Drosophila testis development and that its down-regulation disturbs key signaling pathways related to cell survival and differentiation. These DEPs and DEPPs identified may provide significant candidate set for future studies on the mechanism of male reproduction of animals, including humans.
通讯机构：
[Xiong, YZ; Lenhard, M ] U;[Xiong, YZ ] C;Cent China Normal Univ, Sch Life Sci, Luoyu Ave 152, Wuhan 430079, Peoples R China.;Univ Potsdam, Inst Biochem & Biol, Karl Liebknecht Str 24-25, D-14476 Potsdam, Germany.
关键词：
benzaldehyde;β-ocimene;Brassicaceae;Capsella;floral scent;pollinator attraction;quasi-isogenic line
摘要：
PREMISE OF THE STUDY: Floral scent, usually consisting of multiple compounds, is a complex trait and its role in pollinator attraction has received increasing attention. However, it is difficult to disentangle the effect of individual floral scent compounds due to the complexity of isolating the effect of single compounds by traditional methods. METHODS: Using available quasi-isogenic lines (qILs) generated as part of the original mapping of two floral scent volatile-related loci, CNL1 (benzaldehyde) and TPS2 (β-ocimene), in Capsella, we have generated four genotypes that should only systematically differ in these two compounds. Plants of the four genotypes were introduced into a common garden outside the natural range of C. rubella or C. grandiflora, with individuals of a self-compatible C. grandiflora line as pollen donors, whose different genetic background facilitates the detection of outcrossing events. Visitors to flowers of all five genotypes were compared, and the seeds set during the common-garden period were collected for high-throughput amplicon-based sequencing to estimate their outcrossing rates. KEY RESULTS: Benzaldehyde and β-ocimene emissions were detected in the floral scent of corresponding genotypes. While some pollinator groups showed specific visitation preferences depending on scent compounds, the outcrossing rates in seeds did not vary among the four scent-manipulated genotypes. CONCLUSIONS: The successful construction of scent-manipulated Capsella materials using qILs provides a powerful system to study the ecological effects of individual floral scent compounds under largely natural environments. In Capsella, individual BAld and β-ocimene emission may act as attractants for different types of pollinators. This article is protected by copyright. All rights reserved.
摘要：
BACKGROUND AND AIMS: Morphological matching between flower and pollinator traits has been documented in diverse plant lineages. Indeed, the matching of corolla-tube length and pollinator-tongue length has been cited repeatedly as a classic case of coevolution. However, there are many possible evolutionary routes to trait matching. Our aim here is both to review the evolutionary mechanisms of plant-pollinator trait matching and to investigate a specific case of trait matching/mismatching in a genus of alpine gingers. METHODS: Roscoea gingers with long corolla tubes in the western Himalayas have pollinators with correspondingly long tongues, but the match between corolla-tube and pollinator-tongue lengths is not seen in the eastern Himalayas. Six floral traits were measured, including corolla-tube depth, an internal trait controlling pollinator access to nectar. We calculated coefficients of variation (CVs) and phylogenetically controlled correlation patterns of these traits in six Roscoea species in order to gain possible insights into stabilizing selection and modularization of these traits. KEY RESULTS: The distal (nectar-containing) portion of the corolla tube exhibited lower CVs that did the basal portion. This is consistent with the hypothesis that pollinators mediate stabilizing selection on the distal, but not basal, portion of the corolla tube. This result, combined with phylogenetic data, suggests that the elevated liquid level of nectar in the distal tube evolved subsequent to dispersal into the eastern Himalayan region and loss of long-tongue pollinators. After accounting for phylogeny, corolla-tube length, anther length, style length, and labellum width were all intercorrelated. Corolla-tube depth was not part of this covariational module, however, suggesting separate adaptation to short-tongued pollinators. CONCLUSIONS: The reduction in functional corolla-tube depth in the Roscoea appears to be related to the loss of long-tongue pollinators associated with dispersal to the eastern Himalayas and pollination by short-tongued pollinators. The apparent mismatch between floral tubes and pollinator tongues is a case of cryptic trait matching between flowers and pollinators, underscoring the importance of combining floral anatomy with pollination ecology, in assessing plant-pollinator trait matching.
期刊：
Pesticide Biochemistry and Physiology,2023年194:105516 ISSN：0048-3575
通讯作者：
Yang, Yongbo;Liu, KY
作者机构：
[Xia, Zhichao; Liu, Kaiyu; Wei, Wei; Wang, Haixia; Yang, Yongbo; Pan, Shuang; Liu, Leilei] Cent China Normal Univ, Sch Life Sci, Wuhan 430070, Peoples R China.;[Wei, Wei; Liu, Leilei] Wuhan Univ Bioengn, Appl Biotechnol Ctr, Wuhan 430415, Peoples R China.;[Wang, Ling] Inst Hubei Agr Acad, Wuhan 430070, Peoples R China.;[Xiao, Yutao] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China.;[Bravo, Alejandra] Univ Nacl Autonoma Mexico, Inst Biotecnol, Apdo Postal 510-3, Cuernavaca 62250, Morelos, Mexico.
通讯机构：
[Yang, YB; Liu, KY ] C;Cent China Normal Univ, Inst Entomol, Sch Life Sci, Wuhan 430070, Peoples R China.
关键词：
ABCC2 promoter;Bacillus thuringiensis;GATAe factor;Helicoverpa armigera;Regulation of gene expression
摘要：
Helicoverpa armigera is a worldwide pest that has been efficiently controlled by transgenic plants expressing Bt Cry toxins. To exert toxicity, Cry toxins bind to different receptors located in larval midgut cells. Previously, we reported that GATA transcription factor GATAe activates the expression of multiple H. armigera Cry1Ac receptors in different insect cell lines. Here, the mechanism involved in GATAe regulation of HaABCC2 gene expression, a key receptor of Cry1Ac, was analyzed. HaGATAe gene silencing by RNAi in H. armigera larvae confirmed the activation role of HaGATAe on the expression of HaABCC2 in the midgut. The contribution of all potential GATAe-binding sites was analyzed by site-directed mutagenesis using Hi5 cells expressing a reporter gene under regulation of different modified HaABCC2 promoters. DNA pull-down assays revealed that GATAe bound to different predicted GATA-binding sites and mutations of the different GATAe-binding sites identified two binding sites responsible for the promoter activity. The binding site B9, which is located near the transcription initiator site, has a major contribution on HaABCC2 expression. Also, DNA pull-down assays revealed that all other members of GATA TF family in H. armigera, besides GATAe, HaGATAa, HaGATAb, HaGATAc and HaGATAd also bound to the HaABCC2 promoter and decreased the GATAe dependent promoter activity. Finally, the potential participation in the regulation of HaABCC2 promoter of several TFs other than GATA TFs expressed in the midgut cells was analyzed. HaHR3 inhibited the GATAe dependent activity of the HaABCC2 promoter, while two other midgut-related TFs, HaCDX and HaSox21, also bound to the HaABCC2 promoter region and increased the GATAe dependent promoter activity. All these data showed that GATAe induces HaABCC2 expression by binding to HaGATAe binding sites in the promoter region and that additional TFs participate in modulating the HaGATAe-driven expression of HaABCC2.
通讯机构：
[Xie, GS ] H;[Zhang, ZF ] C;Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China.;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
de novo fatty acid synthesis;fibrillin 7;galactolipids;KAS I;plastoglobule;rice
摘要：
Plastoglobules (PGs) contiguous with the outer leaflets of thylakoid membranes regulate lipid metabolism, plastid developmental transitions, and responses to environmental stimuli. However, the function of OsFBN7, a PG-core fibrillin gene in rice, has not been elucidated. Using molecular genetics and physiobiochemical approaches, we observed that OsFBN7 overexpression promoted PG clustering in rice chloroplasts. OsFBN7 interacted with two KAS I enzymes, namely OsKAS Ia and OsKAS Ib, in rice chloroplasts. Lipidomic analysis of chloroplast subcompartments, including PGs in the OsFBN7 overexpression lines, confirmed that levels of diacylglycerol (DAG), a chloroplast lipid precursor and monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), the main chloroplast membrane lipids, were increased in PGs and chloroplasts. Furthermore, OsFBN7 enhanced the abundances of OsKAS Ia/Ib in planta and their stability under oxidative and heat stresses. In addition, RNA sequencing and real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analyses showed that the expression of the DAG synthetase gene PAP1 and MGDG synthase gene MDG2 was upregulated by OsFBN7. In conclusion, this study proposes a new model in which OsFBN7 binds to OsKAS Ia/Ib in chloroplast and enhances their abundance and stability, thereby regulating the chloroplast and PG membrane lipids involved in the formation of PG clusters.
作者机构：
[Wan, Cuihong; Wan, CH; Zhang, Zheng; Li, Shengjie; Pan, Ni; Peng, Die; Wang, Shaohui] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Wan, Cuihong; Wan, CH; Zhang, Zheng; Li, Shengjie; Pan, Ni; Peng, Die; Wang, Shaohui] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Wan, CH ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
摘要：
Short open reading frame-encoded peptides (SEPs) are generally 2-100 amino acids in length and participate in various biological processes of the organism. The brain is the central hub of life activities, where different regions perform distinct functions. To characterize SEPs in brain regions, we analyzed SEPs in five mouse brain areas, including hippocampus, frontal cortex, temporal cortex, occipital cortex, and parietal cortex, with mass spectrometry-based proteomics. We obtained 1,095 proteins with less than 100 amino acids and identified 373 SEPs. Approximately 83% of these SEPs are reported for the first time. Half of them are encoded by ncRNA, and nearly one-third can find orthology across species. Specific SEPs were identified in each brain region. For example, IP_1018875 was identified in the frontal cortex, possibly related to autophagy and neuronal signaling. These results enrich the proteome of the mouse brain and help facilitate subsequent studies on the function of SEPs.
期刊：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2023年140:104620 ISSN：0145-305X
通讯作者：
Xu-Sheng Liu
作者机构：
[Wang, Gui-Jie; Liu, Xu-Sheng; Wang, Jia-Lin] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Peoples R China.;[Liu, Xu-Sheng] Cent China Normal Univ, Sch Life Sci, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Xu-Sheng Liu] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 152 Luoyu Road, Wuhan, 430079, China
摘要：
BACKGROUND: The insect hemolymph (blood-equivalent fluid), composed of a large number of hemocytes (blood cells) and a variety of soluble immune effectors, is hostile for pathogens including fungi. In order to survive in the insect hemocoel (body cavity), the entomopathogenic fungus (EPF) has evolved two classical coping strategies, namely evasion and suppression of the host immune reactions. However, it remains unclear whether EPF has other ways of coping with host immunity. RESULTS: In this study, we demonstrated that Metarhizium rileyi (an EPF) infection by injection of blastospores into the hemocoel enhanced the plasma antibacterial activity of cotton bollworm (Helicoverpa armigera), which was partially due to the enhanced expression of antimicrobial peptides (AMPs). The early stage of M. rileyi infection induced the translocation of gut bacteria into the hemocoel, where they were subsequently cleared due to the enhanced plasma antibacterial activity. Further, we showed that the enhanced plasma antibacterial activity and AMP expression were attributable to M. rileyi but not the invasive gut bacteria (opportunistic bacteria). Elevated ecdysone (major steroid hormone in insects) levels in the hemolymph at 48h post-M. rileyi infection might contribute to the enhanced expression of AMPs. The fungus-elicited AMPs, such as cecropin 3 or lebocin, exhibited potent inhibitory activity against the opportunistic bacteria but not against hyphal bodies. In addition, the opportunistic bacteria competed with hyphal bodies for amino acid nutrients. CONCLUSIONS: M. rileyi infection induced the translocation of gut bacteria, and then the fungi activated and exploited its host humoral antibacterial immunity to eliminate opportunistic bacteria, preventing them from competing for nutrients in the hemolymph. Unlike the classical strategies, EPF utilizes to evade or suppress host immunity, our findings reveal a novel strategy of interaction between EPF and host immunity. Video Abstract.
期刊：
JOURNAL OF PROTEOME RESEARCH,2023年22(9):2814-2826 ISSN：1535-3893
通讯作者：
Wan, CH
作者机构：
[Wan, Cuihong; Wan, CH; Ding, Yuhe; Gu, Jingyu; Peng, Zhao; Yan, Jiahao; Qin, Lu] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Wan, Cuihong; Wan, CH; Ding, Yuhe; Gu, Jingyu; Peng, Zhao; Yan, Jiahao; Qin, Lu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Wan, CH ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
zebrafish;early life stage;development;maternal-to-zygotic transition;mass spectrometry
摘要：
The early development of zebrafish (Danio rerio) is a complex and dynamic physiological process involving cell division, differentiation, and movement. Currently, the genome and transcriptome techniques have been widely used to study the embryonic development of zebrafish. However, the research of proteomics based on proteins that directly execute functions is relatively vacant. In this work, we apply label-free quantitative proteomics to explore protein profiling during zebrafish's embryogenesis, and a total of 5961 proteins were identified at 10 stages of zebrafish's early development. The identified proteins were divided into 11 modules according to weighted gene coexpression network analysis (WGCNA), and the characteristics between modules were significantly different. For example, mitochondria-related functions enriched the early development of zebrafish. Primordial germ cell-related proteins were identified at the 4-cell stage, while the eye development event is dominated at 5 days post fertilization (dpf). By combining with published transcriptomics data, we discovered some proteins that may be involved in activating zygotic genes. Meanwhile, 137 novel proteins were identified. This study comprehensively analyzed the dynamic processes in the embryonic development of zebrafish from the perspective of proteomics. It provided solid data support for further understanding of the molecular mechanism of its development.
期刊：
American Journal of Botany,2023年110(6) ISSN：0002-9122
通讯作者：
Huang, SQ
作者机构：
[Huang, Shuang-Quan; Huang, SQ; Hao, Kai] Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.;[Hao, Kai] Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable Ut, South China Bot Garden, Guangzhou 510650, Peoples R China.;[Fang, Qiang] Henan Univ Sci & Technol, Coll Agr, Luoyang 471003, Peoples R China.
通讯机构：
[Huang, SQ ] C;Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.
关键词：
Caryophyllaceae;conspecific pollen limitation;generalized flower;heterospecific pollen deposition;pollen fitness;pollen species diversity;seed set;Silene;tolerance and avoidance strategy
摘要：
Abstract Premise Co‐flowering species that have not evolved an avoidance mechanism may have tolerance to heterospecific pollen (HP) deposition as an adaptive strategy to minimize any deleterious effects of HP transfer, but empirical evidence for the tolerance hypothesis remains scarce. Methods To estimate the potential effects of heterospecific pollen deposition (HPD) on female reproductive success, we counted conspecific (CP) and HP pollen grains deposited on stigmas and assessed subsequent seed set of both open‐ and hand‐pollinated flowers in three co‐flowering Silene species with exposed stigmas that usually received numerous HP grains on the elongated receptive area. Results The percentage of HP grains per flower (HP%) varied from 16.6% to 43.0% among three species. Silene chungtienensis had lower HP%, and the CP‐HP relationship was neutral; S. gracilicaulis and S. yunnanensis had a relatively higher HP% with a positive CP‐HP relationship. The effects of CP and HP number on natural seed set were positive for all three species, but HP% had stronger negative effects in S. chungtienensis and S. gracilicaulis. In hand‐pollinated flowers of the three Silene species, seed set did not decrease with HP whether CP was in excess or insufficient, indicating no negative effects of HPD on seed production. Conclusions Consistent with the tolerance hypothesis, our results indicated that species with higher HP interference are likely to be tolerant to an increase in HP%. These species with generalist‐pollinated flowers and exposed large stigmas may benefit from an increase of conspecific pollen deposition, despite the associated increase in heterospecific pollen deposition.
