作者机构：
[Xu, Huan; Liu, Jinlin; Zhang, Li; Zhao, Fan; Qi, Chao; Chen, Yubing] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Liu, Jinlin; Qi, Chao] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Chao Qi; Jinlin Liu] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, Hubei 430079, China
摘要：
Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia, which is a severe and often fatal disease that results in significant economic loss. The means by which A. pleuropneumoniae survives within the host are not clear. High temperature requirement A (HtrA) proteases have been shown to affect cell viability during stressful conditions and are virulence factors in many bacterial species. In this study, we examined the biological role of HtrA during A. pleuropneumoniae infection by analyzing the impact of htrA mutation on virulence-associated phenotypes. We found that htrA mutation had a dramatic impact on stress tolerance. The htrA mutant (delta htrA) displayed a lethal phenotype at elevated temperature (42 ?). Further, delta htrA exhibited increased susceptibility to H2O2-induced oxidative stress when compared to the parental strain (SLW01) and a complementation strain (delta htrA-Compl). Animal infection assays demonstrated that absence of HtrA led to decreased in vivo colonization ability, and delta htrA is less virulent in pigs relative to SLW01. Furthermore, pig competitive infection assays demonstrated fewer blood associated CFUs with delta htrA infection than with SLW01. These results demonstrate HtrA plays a significant role in the survival and growth of A. pleuropneumoniae during stressful conditions, and that immune escape and invasiveness are important to the process of A. pleuropneumoniae infection.
作者机构：
[Zhang, Shuo; Li, Sirong; Zhao, Sheng; Zhou, Zijun; Wei, Hui; Tie, Zuoxiu; Wang, Quan; Zhang, Huigang; Wei, H; Zhang, Yihong] Nanjing Univ, Coll Engn & Appl Sci, Nanjing Natl Lab Microstruct, Jiangsu Key Lab Artificial Funct Mat, Nanjing 210023, Jiangsu, Peoples R China.;[Wan, Cuihong; Wang, Bing] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Liu, Wei; Ye, Meng] China Pharmaceut Univ, Sch Life Sci & Technol, Jiangsu Key Lab Druggabil Biopharmaceut, State Key Lab Nat Med, Nanjing 211198, Jiangsu, Peoples R China.;[Du, Lei; Cui, Ran] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Hubei, Peoples R China.;[Du, Yan; Liu, Quanyi] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China.
通讯机构：
[Wei, H ] N;Nanjing Univ, Coll Engn & Appl Sci, Nanjing Natl Lab Microstruct, Jiangsu Key Lab Artificial Funct Mat, Nanjing 210023, Jiangsu, Peoples R China.;Nanjing Univ, State Key Lab Analyt Chem Life Sci, Sch Chem & Chem Engn, Nanjing 210023, Jiangsu, Peoples R China.;Nanjing Univ, Chem & Biomed Innovat Ctr ChemBIC, Nanjing 210023, Jiangsu, Peoples R China.
摘要：
Nanozyme is a collection of nanomaterials with enzyme-like activity but higher environmental tolerance and long-term stability than their natural counterparts. Improving the catalytic activity and expanding the category of nanozymes are prerequisites to complement or even supersede enzymes. However, the development of hydrolytic nanozymes is still challenged by diverse hydrolytic substrates and following complicated mechanisms. Here, two strategies are informed by data to screen and predict catalytic active sites of MOF (metal-organic framework) based hydrolytic nanozymes: (1) to increase the intrinsic activity by finely tuned Lewis acidity of the metal clusters; (2) to improve the density of active sites by shortening the length of ligands. Finally, as-obtained Ce-FMA-MOF-based hydrolytic nanozyme is capable of cleaving phosphate bonds, amide bonds, glycosidic bonds, and even their mixture, biofilms. This work provides a rational methodology to design hydrolytic nanozyme, enriches the diversity of nanozymes, and potentially sheds light on future evolution of enzyme engineering. Developing hydrolytic nanozymes remains challenging. Here the authors present a rational methodology to design hydrolytic nanozyme by developing a data-informed strategy to screen and identify potential scaffold and active sites of hydrolase-like nanozyme.
期刊：
Journal of Hazardous Materials,2022年435:128951 ISSN：0304-3894
通讯作者：
Hongwei Sun<&wdkj&>Xiaomei Liao
作者机构：
[Wang, Jian; Liu, Congcong; Liao, Xiaomei; Wang, Shaohui] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhang, Lizhi; Sun, Hongwei] Cent China Normal Univ, Inst Environm & Appl Chem, Coll Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Hongwei Sun] K;[Xiaomei Liao] H;Hubei Key Lab of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, PR China<&wdkj&>Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Institute of Environmental & Applied Chemistry, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
摘要：
The inactivation of microorganisms by nanoscale zero-valent iron (nZVI) was extensively reported, but what happens inside the cells is rarely explored. Herein, we revealed that nZVI caused the drastic increase of intracellular iron concentrations, which subsequently catalyzed the Haber-Weiss reaction to produce high levels of endogenous reactive oxygen species (ROSs) and inactivated E. coli cells by oxidative damage of DNA, evidenced by the significantly higher inactivation efficiencies of E. coli mutant strains deficient in iron uptake regulation and DNA repair than the parental strain. The intracellular iron levels, endogenous ROSs levels and the inactivation efficiencies of E. coli were positively correlated. The permeabilized cytomembrane due to the close contact between nZVI and E. coli was responsible for the iron overload. This work demonstrates experimentally for the first time that nZVI causes iron overload and endogenous oxidative stress to inactivate E. coli, thus deepening our knowledge of the nZVI antimicrobial mechanism.
作者：
Wang, Bo;Tong, Ze-Yu;Xiong, Ying-Ze;Wang, Xiao-Fan;Armbruster, W. Scott;...
期刊：
植物多样性：英文版,2022年45(6):748-751 ISSN：2096-2703
通讯作者：
Huang, SQ
作者机构：
[Wang, Bo; Wang, Xiao-Fan] Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China.;[Huang, Shuang-Quan; Xiong, Ying-Ze; Tong, Ze-Yu; Huang, SQ] Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.;[Armbruster, W. Scott] Univ Portsmouth, Sch Biol Sci, Portsmouth PO12DY, England.;[Armbruster, W. Scott] Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK 99775 USA.
通讯机构：
[Huang, SQ ] C;Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.
关键词：
Coefficient of variation;Coiled style;Corolla-tube length;Nectar depth;Pollinator shift;Trait covariation
摘要：
The plant-pollinator 'arms race' model posits that a major driver of the evolution of elongated corollas in flowers is reciprocal selection for 'morphological fit' between pollinator-tongue length and access distance to nectar (usually corolla-tube length). Evidence for the pollinator-mediated selection on tube length and evolution of multiple, correlated floral traits remains inconclusive. To gain possible insights into the strength of stabilizing selection by assessing standing phenotypic variation, we measured a series of functionally important floral traits, including corolla tube length and 'effective' tube depth and degree of style coiling. We then calculated coefficients of variation (CV) for these traits in three field populations of R. schneideriana. Unlike in most long-tubed flowers, the bottom part of the corolla tube is completely occupied by the style, with no room for nectar. The length of this portion of the corolla tube was more variable (higher CV) than the upper part of the corolla tube, suggesting that functional tube depth was under stronger stabilizing selection. The degree of style coiling was negatively related to the corolla-tube length in all three populations of R. schneideriana, suggesting that there may be conflicting selection acting on style length and corolla-tube length, which are otherwise usually tightly correlated. Given the lack of nectar in the flowers of this species, the long corolla tubes and long styles may represent morphological holdovers from ancestors that were pollinated by long-tongued pollinators, as is still seen in related species in the western Himalayas. Copyright (c) 2022 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY
摘要：
Neuronal persistent activity (PA) is a common phenomenon observed in many types of neurons. PA can be induced in neurons in the mouse auditory nucleus by activating cholinergic receptors with carbachol (CCh), a dual muscarinic and nicotinic receptor agonist. PA is presumed to be associated with learning-related auditory plasticity at the cellular level. However, the mechanism is not clearly understood. Many studies have reported that muscarinic receptor agonists inhibit muscarinic-sensitive potassium channels (M channels). Potassium efflux through M channels produces potassium currents, called M currents, that play an essential role in regulating neural excitability and synaptic plasticity. Further study is needed to determine whether M currents affect the PA of auditory central neurons and provide additional analysis of the variations in electrophysiological properties. We used in vitro whole cell patch-clamp recordings in isolated mouse brain slices to investigate the effects of M currents on the PA in pyramidal neurons in layer V of the primary auditory cortex (AI-L5). We found that blocking M currents with XE991 depolarized the AI-L5 pyramidal neurons, which significantly increased the input resistance. The active threshold and threshold intensity were significantly reduced, indicating that intrinsic excitability was enhanced. Our results also showed that blocking M currents with XE991 switched the neuronal firing patterns in the AI-L5 pyramidal neurons from regular spiking to intrinsic bursting. Blocking M currents facilitated PA by increasing the plateau potential and enhancing intrinsic excitability. Our results suggested that blocking M currents might facilitate the PA in AI-L5 pyramidal neurons, which underlies auditory plasticity. NEW & NOTEWORTHY Persistent activity (PA) in AI-L5 pyramidal neurons plays an essential role in acoustic information processing. We used in vitro whole cell patch-clamp recordings to investigate the effects of M currents on the PA in AI-L5 pyramidal neurons. Blocking M currents with XE991 facilitated PA by increasing the plateau potential and enhancing intrinsic excitability, causing the firing patterns of AI-L5 pyramidal neurons to become more bursting. These results provide new insight into our understanding of the cellular mechanisms that govern learning-induced auditory plasticity.
摘要：
BACKGROUND: Due to the rapid rise in arboviral disease cases, there is a need for alternative methods of vector control since fast growing insecticides resistance is a matter of great concern. Recent studies have shown the potential of entomopathogenic fungi in controlling mosquito vectors, but behavioural responses of the mosquitoes encountering with entomopathogenic fungi are still unclear. RESULTS: In this study, behavioural responses induced by the entomopathogenic fungus Metarhizium anisopliae (Metschnikoff) Sorokin in adult female Aedes aegypti mosquito were evaluated. The survival of female mosquitoes was significantly reduced after exposure to medium and high concentrations of fungal conidia. A significant increase in frequencies and durations of different self-grooming types was observed in mosquitoes exposed to medium or high concentrations of fungal conidia. Mosquitoes were able to differentiate between active and inactive fungal conidia as application of inactive conidia showed non-significant effect on survival and self-grooming parameters. A concentration-specific reduction in flight locomotor activity of the female mosquitoes was found after fungal treatments. Fungal-exposed mosquitoes showed significantly higher antifungal activity 72 h post-application. CONCLUSION: These findings provide the better understanding of behavioural responses of the mosquitoes to resist fungal infections and suggest that mosquitoes can remove the lower amounts of fungal conidia through self-grooming behaviour, which they encounter within natural field conditions. This article is protected by copyright. All rights reserved.
期刊：
Science of The Total Environment,2022年852:158319 ISSN：0048-9697
通讯作者：
Shumao Ding
作者机构：
[Zhou, Tingting; Ye, Xin; Wan, Jian; He, Suli; Wei, Zhaolan; Zhang, Hongmao; Ding, Shumao] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.;[Ye, Xin] Sichuan Univ Sci & Engn, Coll Bioengn, Liquor Marking Biol Technol & Applicat Key Lab Sic, Yibin, Peoples R China.
通讯机构：
[Shumao Ding] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Hubei, China
摘要：
Multi-walled carbon nanotubes (MWCNTs) and dibutyl phthalate (DBP) exist extensively in the environment, and they are easy to form compound pollution through π-π interactions in the environment. We investigate whether DBP, an environmental hormone disruptor, mediated by CNTs can more easily cross the blood-brain barrier, and whether DBP entering the brain has neurotoxic effects on the cells in the brain. Experimental subjects were 40 male Kunming (KM) mice randomly divided into 4 groups: the control group; the MWCNTs group; the DBP group; and the MWCNTs+DBP group. The mice were exposed via tail intravenous injection once every 3 days for 21 days, following which toxicology studies were carried out. The results of behavioral experiments showed that the mice in the combined exposure group (MWCNTs+DBP) exhibited spatial learning and memory impairment, and anxiety-like behavior. Staining of hippocampal sections of mouse brain tissue showed that, in the CA1, CA2, and DG areas, the number of neurons decreased, the nucleus was pyknotic, the cell body was atrophied, and levels of the microglia marker Iba-1 increased. By proteomic KEGG analysis, we found that the DEPs were mainly those related to neurodegenerative diseases. Immunohistochemistry in the hippocampus indicated that the level of brain-derived neurotrophic factor (BDNF) in the DG region was significantly increased. RT-PCR results revealed that the expression levels of P53, caspase3, and Bax genes related to apoptosis were up-regulated. The experimental results demonstrated that the mechanism of the combined-exposure injury to neurons in the hippocampus of mice may be that MWCNTs with adsorbed DBP can induce the release of BDNF, accelerate the apoptosis of neurons, and reduce the number of nerve cells, which activates microglia, causing neuroinflammation and nervous system toxicity.
作者机构：
[Luo, Jingwen; Chen, Feng; Xu, Wenliang; Li, Juan; Guo, Yanjun; Qiao, Mengfei] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zeng, Wei] Zhejiang A&F Univ, Coll Forestry & Biotechnol, Sino Australia Plant Cell Wall Res Ctr, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China.
通讯机构：
[Xu, Wenliang] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Cellulose synthesis;Cotton fiber;DUF288 domain containing proteins;Fiber development
摘要：
Cotton is one of the most important economic crops in the world, with over 90 % cellulose in the mature fiber. However, the cellulose synthesis mechanism in cotton fibers is poorly understood. Here, we identified four DUF288 domain containing proteins, which we designated GhSTL1-4. These four GhSTL genes are highly expressed in 6 days post anthesis (dpa) and 20 dpa cotton fibers. They are localized to the Golgi apparatus, and can rescue the growth defects in primary cell wall (PCW) and secondary cell wall (SCW) of cellulose synthesis of the Arabidopsis stl1stl2 double mutant at varying degrees. Silencing of GhSTLs resulted in reduced cellulose content and shorter fibers. In addition, split-ubiquitin membrane yeast two-hybrid analysis showed that GhSTL1 and GhSTL4 can interact with PCW-related GhCesA6-1/6-3 and SCW-associated GhCesA7-1/7-2. GhSTL3 can interact with SCW-related GhCesA4-3. These interactions are further confirmed by firefly luciferase complementation imaging assay. Together, we demonstrate that GhSTLs can selectively interact with both the PCW and SCW-associated GhCesAs and impact on cellulose synthesis and fiber development. Our findings provide insights into the mechanism underlying cellulose biosynthesis in cotton fibers, and offer potential candidate genes to coordinate PCW and SCW cellulose synthesis of cotton fibers for developing elite cotton varieties with enhanced fiber quality.
期刊：
International Journal of Molecular Sciences,2022年23(16):9459- ISSN：1422-0067
通讯作者：
Yu-Feng Wang
作者机构：
[Mao, Bin; Wang, Zhi-Wei; Zhang, Hua-Bao; Yu, Wen-Juan; Wang, Bing; Zong, Qiong; Wang, Yu-Feng] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Yu-Feng Wang] A;School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, China
摘要：
The endosymbiotic Wolbachia bacteria frequently cause cytoplasmic incompatibility (CI) in their insect hosts, where Wolbachia-infected males cross with uninfected females, leading to no or fewer progenies, indicating a paternal modification by Wolbachia. Recent studies have identified a Wolbachia protein, CidB, containing a DUB (deubiquitylating enzyme) domain, which can be loaded into host sperm nuclei and involved in CI, though the DUB activity is not necessary for CI in Drosophila melanogaster. To investigate whether and how Wolbachia affect protein ubiquitination in testes of male hosts and are thus involved in male fertility, we compared the protein and ubiquitinated protein expressions in D. melanogaster testes with and without Wolbachia. A total of 643 differentially expressed proteins (DEPs) and 309 differentially expressed ubiquitinated proteins (DEUPs) were identified to have at least a 1.5-fold change with a p-value of <0.05. Many DEPs were enriched in metabolic pathway, ribosome, RNA transport, and post-translational protein modification pathways. Many DEUPs were involved in metabolism, ribosome, and proteasome pathways. Notably, 98.1% DEUPs were downregulated in the presence of Wolbachia. Four genes coding for DEUPs in ubiquitin proteasome pathways were knocked down, respectively, in Wolbachia-free fly testes. Among them, Rpn6 and Rpn7 knockdown caused male sterility, with no mature sperm in seminal vesicles. These results reveal deubiquitylating effects induced by Wolbachia infection, suggesting that Wolbachia can widely deubiquitinate proteins that have crucial functions in male fertility of their hosts, but are not involved in CI. Our data provide new insights into the regulatory mechanisms of endosymbiont/host interactions and male fertility.
作者机构：
[Luo, Yonghong; Cheng, Jiming; Yan, Xingfu] North Minzu Univ, Coll Biol Sci & Engn, Yinchuan 750021, Peoples R China.;[Luo, Yonghong; Cheng, Jiming; Yan, Xingfu] Natl Ethn Affairs Commiss Peoples Republ China, Key Lab Ecol Protect Agropastoral Ecotones Yellow, Yinchuan 750021, Peoples R China.;[Luo, Yonghong; Zhang, Jingbao] Inner Mongolia Univ, Sch Ecol & Environm, Hohhot 010000, Peoples R China.;[Cheng, Jiming] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.;[Zhang, Jinfeng] Beijing Forestry Univ, Beijing Key Lab Forest Resources & Ecosyst Proc, Beijing 100083, Peoples R China.
通讯机构：
[Jinfeng Zhang; Xingfu Yan] A;Authors to whom correspondence should be addressed.<&wdkj&>Key Laboratory of Ecological Protection of Agro-Pastoral Ecotones in the Yellow River Basin National Ethnic Affairs Commission of the People’s Republic of China, Yinchuan 750021, China<&wdkj&>College of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>Beijing Key Laboratory for Forest Resources and Ecosystem Processes, Beijing Forestry University, Beijing 100083, China
作者机构：
[Zhou, Jiang; Wen, Huamei] Guizhou Normal Univ, Sch Karst Sci, Guiyang 550001, Peoples R China.;[Wen, Huamei; Yan, Shasha] Cent China Normal Univ, Sch Life Sci, Wuhan 430000, Peoples R China.;[Liu, Enshuo] Guizhou Normal Univ, Sch Life Sci, Guiyang 550001, Peoples R China.;[Chang, Jiang] Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China.;[Xiao, Ning] Guiyang Nursing Vocat Coll, Guiyang 550014, Peoples R China.
通讯机构：
[Zhou, J.] S;School of Karst Sciences, China
关键词：
Karst;IUCN Red List;Biodiversity;Conservation
摘要：
Subterranean aquatic biodiversity loss has exceeded that of surface aquatic systems, and cavefish are a predominant indicator of biodiversity loss. The karst subsurface environment and its biodiversity are generally considered to be fragile and extremely sensitive to environmental disturbances such as hydroelectric projects. Subterranean biodiversity is faced with several threats, such as: hydrostation mining, domestic wastewater etc., and protecting and managing these unique and endemic species presents several challenges of wild population survive and monitoring population dynamic. The impact of infrastructure policies on cavefish species in China is used as an example to analyse the current and potential threats to cavefish species and karst subterranean habitats in China. Any strategy employed to conserve cavefish in China should include strengthening research on cavefish, including formal descriptions of taxa and listing these species on the IUCN Red List, as well as national and local lists of species for conservation. Such knowledge can be used to establish a conservation strategy for cavefish and subsurface biodiversity.
作者机构：
[Niu, Wei; Chen, Jiahui; Guo, Xinbiao; Deng, Furong; Wang, Wanzhou] Peking Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth Sci, Beijing 100191, Peoples R China.;[Huang, Chen] Univ Shanghai Sci & Technol, Sch Environm & Architecture, Shanghai 200093, Peoples R China.;[Zhang, Zhihong] Shanxi Med Univ, Sch Publ Hlth, Dept Environm Hlth, Shanxi 030001, Peoples R China.;[Ma, Le; Wu, Shaowei] Xi An Jiao Tong Univ, Sch Publ Hlth, Shaanxi 710061, Peoples R China.;[Li, Rui] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Xinbiao Guo; Furong Deng] D;Department of Occupational and Environmental Health Sciences, School of Public Health, Peking University, Beijing, 100191, China
关键词：
Air pollution;Blood pressure;Heart rate variability;Lung function;Particulate matter;Respirator
摘要：
Wearing a respirator is generally the most convenient individual intervention against ambient particulate matter (PM), and therefore there has been considerable research into its effectiveness. However, the effects of respirator intervention under different PM concentration settings have been insufficiently elucidated. We conducted a randomized, blinded, crossover intervention study in four representative cities in China in which 128 healthy university students spent 2-h walking along a busy road wearing either a real or a sham respirator and then spent the next 5-h indoors away from traffic pollution. Lung function, blood pressure, and heart rate variability were continuously measured throughout the visit. Linear mixed-effect models were fitted to evaluate the protective effects of respirator intervention on the cardiopulmonary indicators. Results showed that the beneficial effects of respirator intervention were only occasionally significant at specific time points or in specific cities or in selected parameters. Overall, respirator intervention was associated with reduced SBP (6.2 vs. 11.5mmHg compared to baseline, p<0.05) and increased LF (44 vs. 35ms(2) compared to baseline, p<0.05) over the 2-h walk, but no significant effects were found over the 7-h period. Respirators have significant effect modifications on the associations between PM(2.5)/PM(10) and the cardiopulmonary indicators, but the directions of effects were inconsistent. The intercity difference in the effects of respirator intervention was found significant, with Taiyuan and Shanghai to be the two cities with lower personal PM concentrations but more pronounced benefits. In conclusion, reducing personal exposure to PM can have some beneficial effects in some scenarios. However, respirators may not provide sufficient protection from air pollution overall, and we should avoid over-reliance on respirators and accelerate efforts to reduce emissions of pollutants in the first place. Despite standardized procedures, we found inconsistency in results across cities, consistent with the previous literature.
作者机构：
[Wei, Tingting; Zheng, Lifang; Zhao, Yuanteng; Lu, Haonan; Li, Rui; Huang, Jiawei; Lu, Xianxian; Lv, Ke; Li, Beibei; Gong, Cunyi] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Lu, Xianxian] Hebei Inst Mech & Elect Technol, Dept Mat & Architectural Engn, Xingtai 054002, Peoples R China.
通讯机构：
[Rui Li] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China
作者机构：
[Mao, Zhifan; Feng, Jiali; Wang, Jiqun; Bao, Lingyuan; Hu, Zelan; Li, Jian; Sun, Tianyue] East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Frontiers Sci Ctr Materiobiol & Dynam Chem,Shangha, Sch Pharm,State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China.;[Huang, Yunyuan] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Xiao, Chaojiang; Li, Jing; Guo, Zhenghan; Shi, Kaixuan; Jiang, Bei; Li, Jian] Dali Univ, Coll Pharm, Yunnan Key Lab Screening & Res Antipathogen Plant, Dali 671000, Yunnan, Peoples R China.;[Liu, Wenwen; Li, Jian] Hainan Univ, Coll Pharm, Key Lab Trop Biol Resources, Minist Educ, Haikou 570228, Hainan, Peoples R China.;[Li, Jian] Tongji Univ, Shanghai Peoples Hosp 10, Clin Med Sci & Tech Innovat Ctr, Sch Med, Shanghai 200092, Peoples R China.
通讯机构：
[Tianyue Sun; Jiqun Wang; Lingyuan Bao; Jiali Feng; Zhifan Mao; Zelan Hu] S;[Jing Li; Zhenghan Guo; Bei Jiang et al.; Kaixuan Shi; Chaojiang Xiao] Y;[Yunyuan Huang] H;Yunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from West Yunnan,College of Pharmacy,Dali University,China<&wdkj&>State Key Laboratory of Bioreactor Engineering,Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism,Frontiers Science Center for Materiobiology and Dynamic Chemistry,China<&wdkj&>Hubei Key Laboratory of Genetic Regulation and Integrative Biology,School of Life Sciences,Central China Normal University,China
关键词：
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
摘要：
The development of safe and effective therapeutic interventions is an important issue for delaying aging and reducing the risk of aging-related diseases. Chinese herbal medicines for the treatment of aging and other complex diseases are desired due to their multiple components and targets. Through screening for effects on lifespan of 836 Chinese herbal medicine extracts, Nicandra physalodes extract (HL0285) was found to exhibit lifespan extension activity in Caenorhabditis elegans (C. elegans). In further experiments, HL0285 improved healthspan, enhanced stress resistance, and delayed the progression of neurodegenerative diseases in C. elegans. Additionally, it ameliorated senescence in human lung fibroblasts (MRC-5 cells) and reversed liver function damage and reduced senescence marker levels in doxorubicin- (Dox-) induced aging mice. In addition, the longevity effect of HL0285 in C. elegans was dependent on the DAF-16 and HSF-1 signaling pathways, as demonstrated by the results of the mutant lifespan, gene level, and GFP level assays. In summary, we discovered that HL0285 had an antiaging effect in C. elegans, MRC-5 cells, and Dox-induced aging mice and deserves to be explored in the future studies on antiaging agents.
期刊：
FRONTIERS IN ONCOLOGY,2022年11:814085 ISSN：2234-943X
通讯作者：
Tong, Jingjing
作者机构：
[Wei, Dingju; Tong, Jingjing; Xu, Meng] Cent China Normal Univ, Sch Life Sci, Wuhan, Peoples R China.;[Wang, Zhihua] Chinese Acad Med Sci, Fuwai Hosp, Shenzhen Key Lab Cardiovasc Dis, Shenzhen, Peoples R China.;[Wang, Zhihua] Chinese Acad Med Sci & Peking Union Med Coll, Fuwai Hosp, Natl Ctr Cardiovasc Dis, State Key Lab Cardiovasc Dis, Beijing, Peoples R China.
通讯机构：
[Tong, Jingjing] C;Cent China Normal Univ, Sch Life Sci, Wuhan, Peoples R China.
关键词：
cancer metabolism;metabolic heterogeneity;metabolic reprogramming;single-cell metabolomics;tumor drug resistance
期刊：
Journal of Molecular Biology,2022年434(2):167404 ISSN：0022-2836
通讯作者：
Jinrong Min<&wdkj&>Ke Liu
作者机构：
[Li, Zhenhua; Chen, Sizhuo; Jia, Lingbo; Zhang, Xiyou; Wu, Zhibin; Liu, Ke; Zhou, Mengqi; Min, Jinrong] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Jinrong Min; Ke Liu] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, PR China
关键词：
*5-methylcytosine DNA binding;*Arabidopsis thaliana;*DNA cytosine methylation;*MBD;*X-ray crystallography
摘要：
Cotton which produces natural fiber materials for the textile industry is one of the most important crops in the world. Class II KNOX proteins are often considered as transcription factors in regulating plant secondary cell wall (SCW) formation. However, the molecular mechanism of the KNOX transcription factor-regulated SCW synthesis in plants (especially in cotton) remains unclear in details so far. In this study, we show a cotton class II KNOX protein (GhKNL1) as a transcription repressor functioning in fiber development. The GhKNL1-silenced transgenic cotton produced longer fibers with thicker SCWs, whereas GhKNL1 dominant repression transgenic lines displayed the opposite fiber phenotype, compared with controls. Further experiments revealed that GhKNL1 could directly bind to promoters of GhCesA4-2/4-4/8-2 and GhMYB46 for modulating cellulose synthesis during fiber SCW development in cotton. On the other hand, GhKNL1 could also suppress expressions of GhEXPA2D/4A-1/4D-1/13A through binding to their promoters for regulating fiber elongation of cotton. Taken together, these data revealed GhKNL1 functions in fiber elongation and SCW formation by directly repressing expressions of its target genes related to cell elongation and cellulose synthesis. Thus, our data provide an effective clue for potentially improving fiber quality by genetic manipulation of GhKNL1 in cotton breeding.
摘要：
The changing climate is one of the most important factors affecting public health. Older people are particularly threatened due to their less efficient immune systems. To evaluate the potential benefits of short-term indoor dehumidification on their circulation and cardiopulmonary health, we conducted a random, cross-over experiment with 36 healthy residents of an aged-care center in Chongqing, China in 2020. Vapor compression dehumidifiers were used over two 48-h periods. At the end of each 48h, we immediately measured sixteen circulatory system biomarkers of inflammation, coagulation, and oxidative stress; lung function; blood pressure; and heart rate. Indoor temperature and relative humidity were monitored throughout the study period. Linear, mixed-effect models were used to associate health endpoints with indoor relative humidity. This intervention study showed that when the indoor relative humidity decreased from 75% to 45%: (1) the coagulation indicators, sCD40l, and PAI-1, decreased significantly, by 58.82% and 23.50%, respectively; (2) the inflammatory indicators, CRP, IL-6, and TNF-α decreased significantly, by 4.09%, 25.78%, and 10.60%, respectively; (3) PEF, FEV(1) and FVC were increased significantly by 20.08%, 14.54%, and 15.75% respectively. To the best of our knowledge, this is the first study to examine the impact of short-term dehumidification on clinical and biochemical measures of cardiorespiratory health in humid areas, and our study suggests that RH in the dehumidified rooms (46.9±8.7%) may be healthier than that in humid rooms (75.2±7.9%). Humidity may be involved in the development of atherosclerosis by activating oxidative stress and mediating the secretion of inflammatory indicators. At the same time, platelet activation induced by oxidative stress stimulates thrombosis to increase cardiovascular risk in older people. Conclusion: This intervention study shows that in a Chinese city like Chongqing with serious indoor environmental humidity, indoor short-term dehumidification has obvious cardiopulmonary benefits for the healthy elderly.
