作者机构：
[Zhang, Jin; Feng, Yingying; Chen, Sizhuo; Liu, Ke; Zhou, Mengqi; Min, Jinrong] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Jinrong Min; Ke Liu] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, PR China<&wdkj&>Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, PR China
期刊：
Journal of Proteomics,2022年266:104681 ISSN：1874-3919
通讯作者：
Cuihong Wan
作者机构：
[Wan, Cuihong; Li, Sige; Yao, Sheng; Zhan, Yuyue] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Wan, Cuihong] 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Cuihong Wan] S;School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, Hubei 430079, People's Republic of China
摘要：
Sulfolobus islandicus is thermophilic archaea that live in an extreme environment of 75°C-80°C and pH2-3. Currently, the molecular mechanism of archaeal adaptation to high temperatures and the stability of proteins at high temperatures are still unclear. This study utilizes proteomics to analyze the differential expression of S. islandicus proteins at different temperatures. We found that ribosomes, glycolysis, nucleotide metabolism, RNA metabolism, transport system, and sulfur metabolism are all affected by temperature. Methylation modification of some proteins changed with temperature. Thermal proteome profiling (TPP) was used to analyze the thermal stability of proteins under 65°C-85°C growth conditions. It is suggested that the T(m) values of proteins are mainly distributed around the optimum growth temperature (OGT). The proteins in the glycolysis pathway had high thermal stability. Meanwhile, proteins related to DNA replication and translation showed low thermal stability. The protein thermal stability of S. islandicus cultured under 65°C and 85°C was higher than that of 75°C. Our study reveals that S. islandicus may adapt to temperature changes by regulating protein synthesis and carbon metabolism pathways, changing post-translational modifications, and improving protein stability at the same time. SIGNIFICANCE: The molecular mechanism of archaeal adaptation to high temperatures and the stability of proteins at high temperatures are still unclear. Our proteomics study identified 477 differentially expressed proteins of S. islandicus at different temperatures, suggesting that ribosomes, glycolysis, nucleotide metabolism, RNA metabolism, transport system, and sulfur metabolism are affected by temperature. Meanwhile, we found that methylation modification of some proteins changed with temperature. To evaluate the thermal stability of the proteome, we performed thermal proteome profiling to analyze the Tm of proteins under 65°C-85°C growth conditions. T(m) values of proteins are mainly distributed around the optimum growth temperature. The proteins in the glycolysis pathway had high thermal stability. Meanwhile, proteins related to DNA replication and translation showed low thermal stability. Our study reveals that S. islandicus may adapt to temperature changes by regulating protein synthesis and carbon metabolism pathways, changing post-translational modifications, and improving protein stability at the same time.
作者机构：
[Wan, Cuihong; Wan, CH] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.;[Wan, Cuihong; Wan, CH] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.
通讯机构：
[Wan, CH ] C;Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.
关键词：
LC/MS/MS;method development;peptidomics;sample preparation;short open reading frame;sORF-encode peptides
摘要：
Accumulating evidence has shown that a large number of short open reading frames (sORFs) also have the ability to encode proteins. The discovery of sORFs opens up a new research area, leading to the identification and functional study of sORF encoded peptides (SEPs) at the omics level. Besides bioinformatics prediction and ribosomal profiling, mass spectrometry (MS) has become a significant tool as it directly detects the sequence of SEPs. Though MS-based proteomics methods have proved to be effective for qualitative and quantitative analysis of SEPs, the detection of SEPs is still a great challenge due to their low abundance and short sequence. To illustrate the progress in method development, we described and discussed the main steps of large-scale proteomics identification of SEPs, including SEP extraction and enrichment, MS detection, data processing and quality control, quantification, and function prediction and validation methods.
通讯机构：
[Hongmao Zhang; Shumao Ding] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Hubei, China
摘要：
The large conjugated π bond in the molecular structure of carbon nanotubes (CNTs) interacts with the benzene ring structure in di (n-butyl) phthalates (DBP) through a π - π bond. Compounds of CNTs and DBP form easily, becoming another environmental pollutant of concern. We explore whether CNTs entering animals slow down the degradation of the DBP adsorbed in the CNT cavity, thereby prolonging the "hormonal activity" of DBP. In our study, male BALb/c mice were used as experimental subjects divided into four groups: the control group; the multi-walled carbon nanotubes (MWCNTs) exposure group (10mg/kg/d); the DBP exposure group (2.15 mg/kg/d); and the compound exposure group (MWCNTs + DBP). After 30 days of exposure, the mice were sacrificed and their spleens used for immunotoxicology study. The results showed that the exposure groups exhibited splenomegaly and suffered severe oxidative damage to the spleen. In the compound exposure group: levels of IgA and IgG in the serum of the mice changed, and were significantly different from levels in both the MWCNTs and DBP exposure groups (p <0.05); the pathological sections of the spleen showed that the boundary between the white pulp area (WP) and the red pulp area (RP) was blurred, that the cell arrangement was loose, and that more red blood cells were retained in the spleen. Proteomics mass spectrometry analysis showed that compared with the control group, 70 proteins were up-regulated and 27 proteins were down-regulated in the MWCNTs group, 36 proteins were up-regulated and 23 proteins were down-regulated in the DBP group, 87 proteins were up-regulated and 21 proteins were down-regulated in the compound exposure group. The results of GO enrichment analysis and KEGG enrichment analysis of the differentially expressed proteins showed that the compound exposure harmed the spleen antigen recognition, processing, and presentation, inhibited the activation and proliferation of B cells and T cells, and hindered the adaptive immune responses. Our results showed that MWCNTs and DBP compounds can damage the spleen, and impair the innate and adaptive immune functions of the body.
期刊：
International Journal of Environmental Research and Public Health,2022年19(13):8150- ISSN：1661-7827
通讯作者：
Yang Yang
作者机构：
[Zhao, Wei; Deng, Wei; Yang, Yang] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Yang Yang] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China<&wdkj&>Author to whom correspondence should be addressed.
期刊：
Science of The Total Environment,2022年845:157364 ISSN：0048-9697
通讯作者：
Jian Han
作者机构：
[Zhu, Biran; Hua, Jianghuan] Hubei Univ Chinese Med, Sch Basic Med Sci, Wuhan 430065, Peoples R China.;[Zhou, Bingsheng; Zhu, Biran; Yang, Lihua; Fu, Kaiyu; Lei, Lei; Han, Jian] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.;[Zhao, Songlin] Cent China Normal Univ, Inst Nanosci & Nanotechnol, Coll Phys Sci & Technol, Wuhan 430079, Peoples R China.;[Li, Rui] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Jian Han] S;State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
关键词：
Chorion;Co-exposure;Neurotoxicity;SiO(2) nanoparticles;Tetrabromobisphenol A
摘要：
Silicon dioxide nanoparticles (n-SiO(2)) absorb tetrabromobisphenol A (TBBPA) and modify its bioavailability and toxicity in the aquatic phase; embryonic chorion is an efficient barrier against nanoparticles (e.g., SiO(2)) and influences their toxicity. However, few studies have investigated developmental neurotoxicity in fish after co-exposure to TBBPA and n-SiO(2), especially considering the barrier function of the chorion. In the present study, zebrafish embryos were exposed to TBBPA (50, 100, and 200 μg/L) alone or in combination with n-SiO(2) (25 mg/L) until 24 or 120 h post fertilization (hpf), in the presence and absence of the chorion. The results confirmed that TBBPA exposure alone significantly downregulated the expression of neurodevelopment marker genes (mbp, alpha-tubulin, shha, and gfap), altered acetylcholinesterase activity and acetylcholine content, and affected locomotor behavior at different developmental stages. Moreover, the results indicated that n-SiO(2) promoted TBBPA-induced neurotoxic effects in zebrafish larvae at 120 hpf, including further repression of the transcription of CNS-related genes, disruption of the cholinergic system, and decrease in the average swimming speed under dark/light stimulation. However, scanning electron microscopy/energy dispersive spectroscopy analysis revealed that at 24 hpf, the embryonic chorion efficiently blocked n-SiO(2) and consequently decreased the bioaccumulation of TBBPA and TBBPA-induced neurotoxicity in dechorionated zebrafish embryos. Taken together, the results demonstrate that n-SiO(2) affected the bioavailability and neurodevelopmental toxicity of TBBPA, and their combined toxicity to zebrafish embryos was mitigated by embryonic chorion, which will facilitate risk assessment on n-SiO(2) and TBBPA and improve understanding the function of the fish embryonic chorion.
作者：
Al Hafiz, Md A.;Liang, X. T.;Yao, Q.;Shen, H.;Nibona, E.;...
期刊：
Inland Water Biology,2022年15(5):674-683 ISSN：1995-0829
通讯作者：
H. B. Zhao
作者机构：
[Zhao, H. B.; Nibona, E.; Wang, B.; Wang, Z. Q.; Yao, Q.; Liang, X. T.; Zhong, X. P.; Zhou, Q. Ch; Al Hafiz, Md A.; Shen, H.] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.;[Nibona, E.] Univ Burundi, Inst Appl Pedag, Bujumbura Burundi, Burundi.
通讯机构：
[H. B. Zhao] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China
关键词：
Medaka;ApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I;mRNA expression;Cholesterol;Growth;Immune stimuli
摘要：
Apolipoprotein A-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I (ApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I) is critical for normal cholesterol metabolism and atheroprotection in mammals. It also involves the immune functions in mammals including humans and fishes. Medaka (Oryzias latipes), a small fish suitable as a model for the study of ApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I. In this study, the expression profile of medaka ApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I (OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I) was done in adult tissues and embryos. Moreover, the response of OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I to dietary cholesterol and immune stimuli was examined. OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I mRNA was expressed in all adult tissues assessed with notably high levels in the intestine and liver. Expression was also detected from the blastula stage to the hatching of embryos using RT-PCR. A cholesterol-rich diet did not affect the growth of young fish but resulted in significant fat deposition in the abdomen. This diet decreased intestinal OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I mRNA levels at first but increased towards the end of the study. Conversely, OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I mRNA in muscle and fat initially increased, then decreased; OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I mRNA was down-regulated in the liver. Further, immune stimulation with lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (PolyI:C) induced up or down-regulation of OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I mRNA in immune organs such as liver, intestine, kidney, and spleen in adult fish. The results suggested that OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I is ubiquitously expressed in adult tissues and is a zygotic gene in medaka embryos. The response of OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I expression to dietary cholesterol and immune stimuli hints a possible role of OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I in cholesterol metabolism and immune response.
期刊：
Proceedings of the National Academy of Sciences of the United States of America,2022年119(27):e2201275119 ISSN：0027-8424
通讯作者：
Li, X.;Luo, J.
作者机构：
[Wang, Huimin; Luo, Jinhong] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhou, Yuxuan; Li, Xingxing] Wuhan Univ, Sch Geodesy & Geomat, Wuhan 430079, Peoples R China.;[Li, Huanhuan] Shanghai KeyGo Technol Co Ltd, Res & Dev Dept, Shanghai 200090, Peoples R China.;[Moss, Cynthia F.] Johns Hopkins Univ, Dept Psychol & Brain Sci, Baltimore, MD 21218 USA.
通讯机构：
[Huimin Wang; Jinhong Luo] H;[Yuxuan Zhou; Xingxing Li] S;Hubei Key Laboratory of Genetic Regulation & Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China<&wdkj&>School of Geodesy and Geomatics, Wuhan University, Wuhan 430079, China
关键词：
auditory feedback;echolocation;vocal production control;Kalman filter;human speech
作者机构：
[Li, Deng-Fei; Yu, Yan; Yang, Hao-Jin; Yan, Xian-Chun] China West Normal Univ, Minist Educ, Key Lab Southwest China Wildlife Resources Conser, Nanchong 637002, Peoples R China.;[Li, Deng-Fei] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Yan, XC ] C;China West Normal Univ, Minist Educ, Key Lab Southwest China Wildlife Resources Conser, Nanchong 637002, Peoples R China.
关键词：
Fruit set;nectar robber;persistent calyx;pollination;Salvia;seed mass
摘要：
The evolution of persistent calyces may be an adaptation to ensure reproductive success of certain flowering plants. However, experimental evidence of the functions of persistent calyces during flowering and seed development remains scarce. We explored the possible functions of persistent calyces in Salvia miltiorrhiza, a perennial herb with campanulate calyx. We conducted calyx manipulation experiments to examine whether persistent calyces affect visitation rates of nectar robbers and pollinators, individual flower longevity, fruit set, seed set and seed mass. Our findings suggested that shortening of the calyx significantly decreased individual flower longevity, fruit set and seed mass, but did not affect visitation of pollinators and nectar robbers. In addition, the seed set of control flowers and the flowers with calyx shortened at the beginning of fruiting stage (CSF flowers) did not significantly differ, but both were higher than that of the flowers with calyx shortened at the beginning of blooming stage (CSB flowers). The seed set and fruit set of CSB flowers were limited by pollination due to the reduction in floral longevity. We conclude that persistent calyces of S. miltiorrhiza may represent adaptive strategies to maintain floral longevity and increase plant fitness. Persistent calyces may provide protection for the growth of flowers and contribute resources to the development of fruits and seeds.
通讯机构：
[Zeng, Y.] B;[Li, J.] J;Brain Science and Advanced Technology Institute, School of Medicine, Wuhan University of Science and Technology, Wuhan 430081, China
摘要：
High-affinity phosphate (Pi) transporters (PHTs) PHT1;1 and PHT1;4 are necessary for plant root Pi uptake especially under Pi-deficient conditions, but how their protein stability is modulated remains elusive. Here, we identified a Ttransfer DNA insertion mutant of Sorting Nexin1 (SNX1), which had more Pi content and less anthocyanin accumulation than the wild type under deficient Pi. By contrast, the snx1-2 mutant displayed higher sensitivity to exogenous arsenate in terms of seed germination and root elongation, revealing higher Pi uptake rates. Further study showed that SNX1 could co-localize and interact with PHT1;1 and PHT1;4 in vesicles and at the plasma membrane. Genetic analysis showed that increased Pi content in the snx1-2 mutant under low Pi conditions could be extensively compromised by mutating PHT1;1 in the double mutant snx1-2 pht1;1, revealing that SNX1 is epistatic to PHT1;1. In addition, SNX1 negatively controls PHT1;1 protein stability; therefore, PHT1;1 protein abundance in the plasma membrane was increased in the snx1-2 mutant compared with the wild type under either sufficient or deficient Pi. Together, our study (i) identifies SNX1 as a key modulator of the plant response to low Pi and (ii) unravels its role in the modulation of PHT1;1 protein stability, PHT1;1 accumulation at the plasma membrane, and root Pi uptake.
摘要：
The most common phenotype induced by the endosymbiont Wolbachia in insects is cytoplasmic incompatibility, where none or fewer progenies can be produced when Wolbachia-infected males mate with uninfected females. This suggests that some modifications are induced in host sperms during spermatogenesis by Wolbachia. To identify the proteins whose phosphorylation states play essential roles in male reproduction in Drosophila melanogaster, we applied isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic strategy combined with titanium dioxide (TiO2) enrichment to compare the phosphoproteome of Wolbachia-infected with that of uninfected male reproductive systems in D. melanogaster. We identified 182 phosphopeptides, defining 140 phosphoproteins, that have at least a 1.2 fold change in abundance with a P-value of <0.05. Most of the differentially abundant phosphoproteins (DAPPs) were associated with microtubule cytoskeleton organization and spermatid differentiation. The DAPPs included proteins already known to be associated with spermatogenesis, as well as many not previously studied during this process. Six genes coding for DAPPs were knocked down, respectively, in Wolbachia-free fly testes. Among them, Slmap knockdown caused the most severe damage in spermatogenesis, with no mature sperm observed in seminal vesicles. Immunofluorescence staining showed that the formation of individualization complex composed of actin cones was completely disrupted. These results suggest that Wolbachia may induce wide changes in the abundance of phosphorylated proteins which are closely related to male reproduction. By identifying phospho-modulated proteins we also provide a significant candidate set for future studies on their roles in spermatogenesis.
