关键词：
hemoglobin;carbon-coated iron nanoparticles;hydrogen peroxide;biosensor;DIRECT ELECTRON-TRANSFER;GLASSY-CARBON ELECTRODE;HORSERADISH-PEROXIDASE;HEME-PROTEINS;ELECTROCATALYSIS;FILMS;BIOSENSOR;CHITOSAN;POLYMER;GLUCOSE
摘要：
A novel method for preparation of hydrogen peroxide biosensor was presented based on immobilization of hemoglobin (Hb) on carbon-coated iron nanoparticles (CIN). CIN was firstly dispersed in a chitosan solution and cast onto a glassy carbon electrode to form a CIN/chitosan composite film modified electrode. Hb was then immobilized onto the composite film with the cross-linking of glutaraldehyde. The immobilized Hb displayed a pair of stable and quasireversible redox peaks and excellent electrocatalytic reduction of hydrogen peroxide (H2O2), which leading to an unmediated biosensor for H2O2. The electrocatalytic response exhibited a linear dependence on H2O2 concentration in a wide range from 3.1 mu M to 4.0 mM with a detection limit of 1.2 mu M (S/N = 3). The designed biosensor exhibited acceptable stability, long-term life and good reproducibility.
作者机构：
[Jiang, Hua] Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, CAS Key Lab Photochem, Beijing 100080, Peoples R China.;Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.;Chinese Acad Sci, Grad Sch, Beijing, Peoples R China.;Capital Normal Univ, Dept Chem, Beijing 100037, Peoples R China.
摘要：
A water-soluble fluorescent sensor, 1, based on the quinoline platform, demonstrates femtomolar sensitivity for zinc ion with a 14-fold enhanced quantum yield upon chelation to zinc ion and also exhibits high selectivity to zinc ion over other physiological relevant divalent metals in the presence of EDTA. X-ray crystal structure of zinc complex reveals that an acetic carboxylic group participates in coordination, which significantly enhances the affinity of 1 for zinc ion.
作者机构：
[Wang, Yan-Gang] Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.;Guangzhou Univ Tradit Chinese Med, Coll Tradit Chinese Med, Guangzhou 510405, Peoples R China.
通讯机构：
[Wang, Yan-Gang] C;Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.
作者机构：
Wuhan Univ Technol, Sch Mat & Technol, Wuhan 430070, Peoples R China.;Cent China Normal Univ, Coll Chem, Wuhan 430079, Hubei, Peoples R China.;Anyang Inst Technol, Dept Chem Engn, Anyang 455000, Henan, Peoples R China.;[Niu Y.-S.] Department of Chemical Engineering, Anyang Institue of Technology, Anyang, Henan 455000, China;[Xiong C.-X.] School of Materials and Technology, Wuhan University of Technology, Wuhan, Hubei 430070, China
通讯机构：
[Xiong, C.-X.] S;School of Materials and Technology, Wuhan University of Technology, China
摘要：
In the title compound, C19H14O3, two independent molecules exist in the enol form with normal values for bond lengths and angles. The crystal packing is stabilized by van der Waals forces.
作者机构：
[Zheng, Aihua] Cent China Normal Univ, Minist Educ, Key Lab Pesticides & Chem Biol, Wuhan 430079, Peoples R China.;Yunyang Med Coll, Dept Med Chem, Shiyan 442000, Peoples R China.
通讯机构：
[Zheng, Aihua] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticides & Chem Biol, Wuhan 430079, Peoples R China.
摘要：
A tandem aza-Wittig reaction of ethyl 3-triphenylphosphoranylideneamino-4- cyano-1-phenyl-1H-pyrrole-2-carboxylate with 4-chlorophenyl isocyanate and diisobutylamine produced the title compound, C27H28ClN5O. In the molecule, the angles between the fused-ring system and the two benzene rings are 51.66 (2) and 65.79 (2)degrees. Intramolecular C-H center dot center dot center dot N hydrogen bonds determine the conformation of the molecule. The packing of the molecules in the crystal structure is governed mainly by intermolecular C-H center dot center dot center dot N hydrogen-bonding interactions. There are no pi-pi interactions.
摘要：
In this paper, we prepared TiO2@CdS core-shell nanorods films electrodes using a simple and low-cost chemical bath deposition method. The core-shell nanorods films electrodes were characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and UV-vis spectrometry techniques. After applying these TiO2@CdS core-shell nanorods electrodes in photovoltaic cells, we found that the photocurrent was dramatically enhanced, comparing with those of bare TiO2 nanorods and US films electrodes. Moreover, TiO2@CdS core-shell nanorods film electrode showed better cell performance than US nanoparticles deposited TiO2 nanoparticles (P25) film electrode. A photocurrent of 1.31 mA/cm(2), a fill factor of 0.43, an open circuit photovoltage of 0.44 V, and a conversion efficiency of 0.8% were obtained under an illumination of 32 mW/cm(2), when the US nanoparticles deposited on TiO2 nanorods film for about 20 min. The maximum quantum efficiency of 5.0% was obtained at an incident wavelength of 500 rim. We believe that TiO2@CdS core-shell heterostructured nanorods are excellent candidates for studying some fundamental aspects on charge separation and transfer in the fields of photovoltaic cells and photocatalysis. (c) 2006 Elsevier B.V. All rights reserved.
作者机构：
[Wan, Jian] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, CCNU, Minist Educ,Coll Chem, Wuhan 430079, Peoples R China.;Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Coll Chem & Chem Engn, Ctr Theoret Chem, Xiamen 361005, Peoples R China.;Wuhan Univ, Basic Med Sch, Dept Pharmacol, Wuhan 430071, Peoples R China.
通讯机构：
[Wan, Jian] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, CCNU, Minist Educ,Coll Chem, Wuhan 430079, Peoples R China.
摘要：
3-Hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) catalyzes the formation of mevalonate. In many classes of organisms, this is the committed step leading to the synthesis of essential compounds, such as cholesterol. However, a high level of cholesterol is an important risk factor for coronary heart disease, for which an effective clinical treatment is to block HMGR using inhibitors like statins. Recently the structures of catalytic portion of human HMGR complexed with six different statins have been determined by a delicate crystallography study (Istvan and Deisenhofer Science 2001, 292, 1160-1164), which established a solid basis of structure and mechanism for the rational design, optimization, and development of even better HMGR inhibitors. In this study, three-dimensional quantitative structure-activity relationship (3D QSAR) with comparative molecular field analysis (CoMFA) was performed on a training set of up to 35 statins and statin-like compounds. Predictive models were established by using two different ways: (1) Models-fit, obtained by SYBYL conventional fit-atom molecular alignment rule, has cross-validated coefficients (q(2)) up to 0.652 and regression coefficients (r(2)) up to 0.977. (2) Models-dock, obtained by FlexE by docking compounds into the HMGR active site, has cross-validated coefficients (q(2)) up to 0.731 and regression coefficients (r(2)) up to 0.947. These models were further validated by an external testing set of 12 statins and statin-like compounds. Integrated with CoMFA 3D QSAR predictive models, molecular surface property (electrostatic and steric) mapping and structure-based (both ligand and receptor) virtual screening have been employed to explore potential novel hits for the HMGR inhibitors. A representative set of eight new compounds of non-statin-like structures but with high pIC(50) values were sorted out in the present study.
期刊：
Journal of Physical Chemistry C,2007年111(18):6832-6836 ISSN：1932-7447
通讯作者：
Zhang, Lizhi
作者机构：
[Zhang, Lizhi] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.;Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China.
通讯机构：
[Zhang, Lizhi] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
摘要：
In this paper, a Bi2WO6 nanoplate film electrode was prepared by a hydrothermal method, combined with a spin coating technique. A photoelectrochemical (PEC) oxidation system based on this electrode was then constructed to degrade rhodamine B (RhB) in aqueous solution under visible light (λ > 420 nm). The PEC system based on Bi2WO6 nanoplate film electrode could degrade 87.2% of RhB with concentration of 5 mg/L in 120 min, operated at low voltage and under visible light irradiation, whereas only 36.8% and 39.5% degradation of RhB were observed for electro-oxidation process (EC) and photocatalytic oxidation process (PC), respectively, operated under the same condition. These results revealed a significant synergetic effect on degrading RhB via electro-oxidation and photocatalysis under visible light irradiation. A possible mechanism for this synergetic degradation of RhB was proposed on the basis of characterization results. Meanwhile, the degradation pathway of RhB during PEC under visible light irradiation was also discussed. This study provided an effective approach for aqueous organic pollutant removal by utilizing solar light.
