作者机构：
[Yang, Guang-Fu; Li, Jing; Zhao, Pei-Liang] Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Wuhan, 430079, China
通讯机构：
[Yang, GF ] ;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
期刊：
Bulletin of Environmental Contamination and Toxicology,2007年78(1):61-65 ISSN：0007-4861
通讯作者：
Wang, X.-D.
作者机构：
[Wang, X.-D.] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, X.-D.] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Biosorption;Algal Cell;Anhydrous Sodium Sulfate;Relative Standard Deviation;Organophosphorus Pesticide
作者机构：
[Liu, Hiu; Li, Chang-Feng; Xiao, Wen-Jing; Liao, Jie; Cao, Yi-Ju; Liu, Xiao-Peng] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.;[Li, Chang-Feng] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, 152 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Li, Chang-Feng] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, 152 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China.
摘要：
An enantioselective organocatalytic intramolecular ring-closing Friedel-Crafts-type alkylation of indolyl alpha,beta-unsaturated aldehydes has been developed. This powerful new strategy allows enantioselective access to THPIs and THBCs in a straightforward and atom-economical manner.
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2007年55(5):1871-1880 ISSN：0021-8561
通讯作者：
He, Hong-Wu
作者机构：
[He, Hong-Wu] Cent China Normal Univ, Minst Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.
通讯机构：
[He, Hong-Wu] C;Cent China Normal Univ, Minst Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
摘要：
The interactions between plasmid DNA and cationic polymers are of interest for their potential biological applications. In this paper, the interactions of DNA with polyamidoamine (PAMAM) dendrimer were studied by fluorescence spectroscopy and stopped-flow technique. A rapid and reproducible fluorescent assay method had been developed for assessing PAMAM and DNA interactions using [Ru(phen)(2)dppZ](2+) as a probe. We further studied the kinetics of PAMAM binding to DNA and the reverse process of DNA dissociation from the complexes. The results indicated that DNA condensation was the rate-determining step during the complexation process, while DNA unfolding and expansion was the rate-determining step during the DNA dissociation process. At N/P ratios before reaching the thermodynamically most stable state, the complexes of DNA and PAMAM were incompact and could dissociate to some extent. And at N/P ratios above 2.0, DNA was fully condensed by PAMAM and dissociation was increasingly difficult. These results provided some useful instructions for self-assembling and disassembling of DNA as well as efficient gene delivery applications. (c) 2006 Elsevier B.V. All rights reserved.
摘要：
Strobilurin derivatives have become one of the most important classes of agricultural fungicide due to a novel action mode, wide fungicidal spectrum, lower toxicity toward mammalian cells, and environmentally benign characteristics. To discover new strobilurin analogues with high activity against resistant pathogens, a series of new chalcone-based strobilurin derivatives are designed and synthesized by integrating a chalcone scaffold with a strobilurin pharmacophore. The preliminary bioassay showed that some of the chalcone analogues exhibited good in vivo fungicidal activities against Pseudoperoniospora cubensis and Sphaerotheca fuliginea at the dosage of 200 μg mL-1. Two compounds, (E)-methyl 2-[2-({3-[(E)-3-(2-chlorophenyl)acryloyl]phenoxy}methyl)phenyl]-3-methoxyacrylate (1e) and (E)-methyl 2-[2-({3-[(E)-3-(3-bromophenyl)acryloyl]phenoxy}methyl)phenyl]-3-methoxyacrylate (1l), were found to display higher fungicidal activities against P. cubensis (EC90 = 118.52 μg mL-1 for 1e and EC90 = 113.64 μg mL-1 for 1l) than Kresoxim-methyl (EC90 = 154.92 μg mL-1) and were identified as the most promising candidates for further study. The present work demonstrated that strobilurin analogues containing chalcone as a side chain could be used as a lead structure for further developing novel fungicides. To our knowledge, this is the first report about the syntheses and fungicidal activities of chalcone-based strobilurin derivatives.
Keywords: Strobilurins; fungicide; chalcone; structure−activity relationships
作者机构：
[杨劭; 杨娇艳; 肖敏] College of Life Sciences, Central China Normal University, wuhan, 430079, China;[肖文精] College of Chemistry, Central China Normal University, wuhan, 430079, China
通讯机构：
[YANG, S.] C;College of Life Sciences, , wuhan, 430079, China
摘要：
It is well known that the wild type Cu,Zn superoxide dismutase (holo SOD) catalyzes the conversion of superoxide anion to peroxide hydrogen and dioxygen. However, a new function of holo SOD, i.e., nucleolytic activity has been found [W. Jiang, T. Shen, Y. Han, Q. Pan, C. Liu, J. Biol. Inorg. Chem. 11 (2006) 835-848], which is linked to the incorporation of exogenous divalent metals into the enzyme-DNA complex. In this study, the roles of exogenous divalent metals in the nucleolytic activity were explored in detail by a series of biochemical experiments. Based on a non-equivalent multi-site binding model, affinity of a divalent metal for the enzyme-DNA complex was determined by absorption titration, indicating that the complex can provide at least a high and a low affinity site for the metal ion. These mean that the holo SOD may use a "two exogenous metal ion pathway" as a mechanism in which both metal ions are directly involved in the catalytic process of DNA cleavage. In addition, the pH versus DNA cleavage rate profiles can be fitted to two ionizing-group models, indicating the presence of a general acid and a general base in catalysis. A model that requires histidine residues, metal-bound water molecules and two hydrated metal ions to operate in concert could be used to interpret the catalysis of DNA hydrolysis, supported by the dependences of loss of the nucleolytic activity on time and on the concentration of the specific chemical modifier to the histidine residues on the enzyme. (c) 2007 Elsevier Inc. All rights reserved.
作者机构：
[Peng, Jianxin] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Inst Entomol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Peng, Jianxin] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
关键词：
AfMNPV;Caspase 3;Cyclosporin A;Cytochrome c;Spodoptera litura cell line (SL-ZSU-1, SL-1)
摘要：
We investigated the influence of cytochrome c on apoptosis induced by Anagrapha (Syngrapha) falcifera multiple nuclear polyhedrosis virus (AfMNPV). Microscopic observation revealed that infection of SL-1 cells with AfMNPV resulted in apoptosis, displaying apoptotic bodies in fluorescent-stained nuclei of AfMNPV-infected SL-1cells. Western blot analysis demonstrated that AfMNPV-induced apoptosis in insect SL-1 cells was significantly inhibited by cyclosporin A which blocked a translocation of cytochrome c from the mitochondria to the cytosol. As determined by using AC-DEVD-AFC as substrate, the activity of caspase-3 in AfMNPV-induced cells was detected as early as 4 h post infection, gradually increased with time extension, and reached a highest level after 16 h of infection. However, activity of caspase-3 in apoptotic cells decreased in the presence of cyclosporin A (30 mu M), indicating that activation of caspase-3 in SfaMNPV-induced cells was dependent on the release of cytochrome c from the mitochondria. In addition, cyclosporin A could markedly inhibit mitochondrial transmembrane potential (Delta Psi m) disruption in undergoing apoptotic cells. These data indicate that cytochrome c plays a key role in AfMNPV-induced apoptosis in S. litura cells and may be required for caspase activation during the induction of apoptosis. (C) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
作者机构：
[Chen, Yunfeng] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.;Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA.
通讯机构：
[Chen, Yunfeng] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
摘要：
Chiral molecular clips constitute a robust system for crystal engineering studies and undergo several levels of orthogonal organization including heterochiral dimerization, H-bond or metal-ligand mediated tape formation, and longitudinal packing of the tapes.
期刊：
JOURNAL OF SEPARATION SCIENCE,2007年30(15):2506-2512 ISSN：1615-9306
通讯作者：
Wang, Xuedong
作者机构：
[Wang, Xuedong] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, Xuedong] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
作者机构：
Univ Ottawa, Dept Chem, Ctr Catalysis Res & Innovat, Ottawa, ON K1N 6N5, Canada.;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.;[Yang, Qian] Univ Ottawa, Dept Chem, Ctr Catalysis Res & Innovat, 10 Marie Curie, Ottawa, ON K1N 6N5, Canada.
通讯机构：
[Yang, Qian] U;Univ Ottawa, Dept Chem, Ctr Catalysis Res & Innovat, 10 Marie Curie, Ottawa, ON K1N 6N5, Canada.
摘要：
A series of new pyrrolidine derivatives were prepared directly in very good yields, from the substrates containing a basic or nucleophilic N atom via ring-closing enyne metathesis reaction under mild reaction conditions. Moreover, the reaction occurs smoothly without the presence of ethylene gas.
期刊：
Bulletin of Environmental Contamination and Toxicology,2007年78(5):368-372 ISSN：0007-4861
通讯作者：
Wang, X.-D.
作者机构：
[Wang, X.-D.] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Col Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, X.-D.] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
