摘要：
Viruses are ubiquitous in human life. Some viruses can be used as vectors of genetic engineering and specific pesticides. Other viruses trigger a variety of diseases in humans, animals and plants, resulting in high infection rates and mortality. Therefore, convenient, accurate and rapid detection of viruses is of great significance for the diagnosis and treatment of subsequent diseases. In contrast to traditional methods of detection, which rely on time-consuming and complex techniques such as polymerase chain reaction (PCR), fluorescent probes and imaging methods generate real-time results, with high specificity, and have been widely used in viral detection. In this review, the application of viral fluorescent probes in analyzing the molecular structure, detection and biological imaging is discussed. In particular, we catego-rized the probes based on their specificity for human and plant viruses, reviewing the latest findings and analyzing their limitations. The potential of fluorescent molecular probes in the treatment of viral dis-ease and environmental analysis, and their possible combinations with protein and immune technology are discussed.(c) 2023 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
作者：
Li Li;Xiaoxie Ma;Yusong Peng;Jun Yin;Nida El Islem Guissi;...
期刊：
ACS Applied Bio Materials,2023年6(4):1639-1649 ISSN：2576-6422
通讯作者：
Jun Yin<&wdkj&>Nida El Islem Guissi<&wdkj&>Yiqing Wang
作者机构：
[Li Li; Yusong Peng; Nida El Islem Guissi; Yiqing Wang] Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, Nanjing 210023, People’s Republic of China;[Xiaoxie Ma; Jun Yin] Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, People’s Republic of China
通讯机构：
[Jun Yin] K;[Nida El Islem Guissi; Yiqing Wang] D;Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, Nanjing 210023, People’s Republic of China<&wdkj&>Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, People’s Republic of China
摘要：
Fluorescent probes with fluorescence emission in the NIR-II window have been widely studied due to increased imaging depth. However, the currently reported NIR-II fluorescent probes present some disadvantages, such as complicated synthesis routes and low fluorescence quantum yields (QYs). The shielding strategy has been used in the development of NIR-II probes to improve their QYs. So far, this strategy has only been used for the symmetric NIR-II probes, especially those based on the benzo[1,2-c:4,5-c']bis([1,2,5]thiadiazole) (BBTD) skeleton. This work reports the synthesis of a series of asymmetric NIR-II probes based on shielding strategies accompanied by simple synthetic routes, high synthetic yields (above 90%), high QYs, and large Stoke shifts. Furthermore, the use of d-α-tocopheryl polyethylene glycol succinate (TPGS) as a surfactant for an NIR-II fluorescence probe (NT-4) improved its water solubility. In vivo studies showed that TPGS-NT-4 NPs with a high QY (3.46%) achieve high-resolution angiography and efficient local photothermal therapy, while displaying good biocompatibility. Hence, we combined angiography and local photothermal therapy to improve the tumor uptake of nanophotothermal agents while reducing their damage to normal tissues.
摘要：
An amphiphilic AIE photosensitizer has been successfully developed, which allows for easily inserting into the bacterial membranes. Binding experiments with phospholipid preliminary demonstrates its membrane specificity. As expected, it is proved to possess a broad-spectrum bacterial staining performance and photodynamic antibacterial activity toward S. aureus and E. coli.
作者机构：
[Yin, Jun; Yang, Guang-Fu; Zeng, Xiaoyan; Yang, GF; Li, Biao; Hua Liu, Sheng; Dong, Jin; Ma, Xiaoxie] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.
通讯机构：
[Yin, J; Yang, GF ] C;Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.
摘要：
4-Hydroxyphenylpyruvate dioxygenase (HPPD) plays a crucial role in the synthesis of nutrients needed to maintain optimal plant growth. Its level is closely linked to the extent of abiotic stress experienced by plants. Moreover, it is also the target of commercial herbicides. Therefore, labeling of HPPD in plants not only enables visualization of its tissue distribution and cellular uptake, it also facilitates assessment of abiotic stress of plants and provides information needed for the development of effective environmentally friendly herbicides. In this study, we created a method for fluorescence labeling of HPPD that avoids interference with the normal growth of plants. In this strategy, a perylene-linked dibenzyl-cyclooctyne undergoes strain-promoted azide-alkyne cycloaddition with an azide-containing HPPD ligand. The activation-based labeling process results in a significant emission enhancement caused by the change in the fluorescent forms from an excimer to a monomer. Notably, this activated bioorthogonal strategy is applicable to visualizing HPPD in Arabidopsis thaliana, and assessing its response to multiple abiotic stresses. Also, it can be employed to monitor in vivo levels and locations of HPPD in crops. Consequently, the labeling strategy will be a significant tool in investigations of HPPD-related abiotic stress mechanisms, discovering novel herbicides, and uncovering unknown biological functions.
摘要：
Changes in cellular viscosity are associated with various physiological processes and pathological conditions. To study these cellular processes and functions, highly sensitive fluorescent probes that detect subtle changes in viscosity are urgently needed but remain lacking. In this study, we present a series of viscosity-responsive near-infrared (NIR) fluorescent probes based on styrene-coated boron dipyrromethene (BODIPY). The probe modified with dimethylaminostyrene and piperazine at the two terminals of the BODIPY scaffold showed extremely high viscosity sensitivity values (x, around 1.54), with excellent performance for detecting viscosity below 20 cP. This outstanding property is attributed to the synergistic effects of multiple rotatable bonds and hydrogen-bond interactions. Additionally, this probe has been successfully deployed to monitor viscosity changes in various cellular compartments (i.e., cytoplasm) and processes (such as during autophagy). This work provides a rational molecular design strategy to construct fluorescent probes with high viscosity sensitivity for exploring cell functions.
作者机构：
[Gao C.; Qiu M.; Yin J.; Ma X.; Huang Y.] Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan, 430079, China;[Xie Y.] Guangdong Provincial Key Laboratory of Radioactive and Rare Resource Utilization, Shaoguan, 512026, China
通讯机构：
[Jun Yin] K;Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan, 430079, China
摘要：
Cell wall is a basic component of bacteria that promotes bacteria to adapt the complicated environment as well as play an essential role in antimicrobial resistance. The structure of bacterial cell wall is remarkably rich, and some complex components, such as peptidoglycan, lipopolysaccharide, and peptidoglycan-arabinogalactan et al., can only be found in bacteria. Furthermore, the biosynthesis and transfer of these glycolipids are indispensable for bacteria during cell elongation. And the process of biosynthesis and transfer are generally associated with metabolism and sophisticated enzyme mechanisms. However, how the metabolic process takes place, what role enzymes play in this process and how they function have been major concerns for scientists in this field.Numerous significant progresses on fluorescent probes and biological imaging bring opportunity for the studying of metabolism and enzyme mechanisms recently due to its non-invasive and high sensitivity. In this review, we focused on metabolic fluorescent probes for the detection of glycolipids in bacterial cell wall and highlighted the involving mechanisms and biological application.
作者机构：
[Huang, Yurou; Yin, Jun; Zeng, Xiaoyan; Liang, Yuting; He, Tian; Liao, Yuanyuan] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.
通讯机构：
[Yin, J ] C;Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.
摘要：
Due to their excellent fluorescence properties and biological function, cyanine dyes have been widely applied in biological imaging. Heptamethine cyanine (Cy7) dyes, as a type of classic near-infrared (NIR) fluorescent dyes, are considered as one of the effective fluorescent tools in the living organisms due to their good biocompatibility and very low background interference. Bioorthogonal reactions performed in living cells and tissues have developed by leaps and bounds in recent years. The NIR fluorescent labeling technique involving cyanine has attracted widespread attention. This review summarizes their recent application in the field of bioorthogonal imaging, mainly concluding Cy7-type dyes, labeling strategy, bioimaging application, etc . We expect this work can provide some helps for the studies of NIR bioorthogonal reaction in vivo .(c) 2023 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
作者机构：
[Huang, Yurou; Yang, Guang-Fu; Liu, Sheng Hua; Chen, Weijie; Yin, Jun; Liu, Jia; Ma, Xiaoxie] Minist Educ, Key Lab Pesticide & Chem Biol, Hubei Int Sci & Technol Cooperat Base Pesticide &, Wuhan, Peoples R China.;[Huang, Yurou; Yang, Guang-Fu; Liu, Sheng Hua; Chen, Weijie; Yin, Jun; Liu, Jia; Ma, Xiaoxie] Int Joint Res Ctr Intelligent Biosensing Technol &, Wuhan, Peoples R China.;[Huang, Yurou; Yang, Guang-Fu; Liu, Sheng Hua; Chen, Weijie; Yin, Jun; Liu, Jia; Ma, Xiaoxie] Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.
通讯机构：
[Prof. Jun Yin; Prof. Guang-Fu Yang] K;Key Laboratory of Pesticide and Chemical Biology (Ministry of Education), Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan, 430079 P. R. China
摘要：
The cationic nature of heptamethine cyanines gives them the capacity to form aggregates with salts by electrostatic interactions. In this work, NaCl promoted J-aggregate formation of aza-coating heptamethine cyanines is explored. NaCl can induce the N-benzyloxycarbonyl Cy-CO2Bz to assemble into a J-aggregate having an absorption at 890 nm. Its excellent fluorescence response to NaCl implies that it has great potential for use as a probe for tracing salt stress in plants. Moreover, NaCl also promotes formation of J-aggregates from the N-ethyloxycarbonyl Cy-CO2Et. The aggregate shows an intense absorption at 910 nm compared to the monomer which absorbs at 766 nm. Its J-aggregated form can serve as a photothermal agent. And the photothermal conversion efficiency is increased from 29.37 % to 57.59 %. This effort leads to the development of two applications of new cyanine J-aggregates including one for tracing salt stress of plants and the other for promoting photothermal therapy of tumors.
摘要：
Diarylethene (DAE) photoswitches, known for their reversible photoinduced cyclization and cycloreversion reactions, have shown great promise in various fields such as optical data storage, optoelectronic devices, supramolecular self-assembly, anti-counterfeiting, chiral photoswitches, and photocontrolled catalysis. Recently, there has been a growing interest in utilizing DAEs as smart triggers to regulate biological events, highlighting their advantages over commonly studied azobenzene switches, including thermal irreversibility, high photoswitching efficiency, favorable cellular stability, and low toxicity. However, a comprehensive review specifically focusing on the biological applications of DAEs has yet to be reported. In this in-depth review, we present key modification strategies aimed at enhancing the photoswitching behavior of DAEs and optimizing their performance for biological applications. These strategies include achieving near-infrared (NIR) photochromism, enhancing aggregation-induced emission (AIE) performance, improving water solubility, enabling fluorescence "turn-on," increasing cyclization yield, and enabling visible light triggering. Moreover, we highlight recent advancements in the use of DAEs as photoswitches in biological applications, covering areas such as photoswitchable fluorescence sensing and imaging, super-resolution imaging, photoswitchable nucleosides and oligonucleotides, photocontrolled photodynamic therapy, and regulation of biological activity in photopharmacology. Furthermore, we identify the current challenges hindering the bioapplications of DAEs and forecast the development trends of photochromic DAEs for potential biological applications. This comprehensive review aims to inspire further research and innovation in the development of novel DAE derivatives for future in vivo bioapplications.
作者机构：
[Huang, Yurou; Yang, Guang-Fu; Yin, Jun; Zeng, Xiaoyan; Liu, Sheng Hua; Li, Anning; Ma, Xiaoxie] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.
通讯机构：
[Yin, J ] C;Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.
摘要：
Cyanines in the near-infrared region are a typical example of a classic fluorescent dye that has garnered significant attention and widespread use in the life sciences and biotechnology. Their character to form assemblies or aggregates has inspired the development of various functional cyanine dye aggregates in phototherapy. This article provides a brief summary of the strategies used to prepare these cyanine dye aggregates. The reports in this concept suggest that the self-assembly of cyanine dyes can enhance their photostability, opening up new possibilities for their application in phototherapy. This concept may encourage researchers to explore the development of functional fluorescent dye aggregates further.
摘要：
The conventional fluorescent probe for sensitive detection of bioactive substance in living cells are still challenging due to the degradation and destruction of lysosomes to probes. Here, we develop a new method based on escape lysosome technology for detection of bioactive small molecules like hydrogen sulfide (H2S) in living cells. It relies on the swelling effect of polymeric nonomicelles. Specifically, PEG-PEI/DP-NBD complex probes were designed and prepared by integrating polyethyleneimine (PEI) and fluorescent probe dansylpiperazine nitrobenzo(c)-(1,2,5) oxadiazole (DP-NBD) into poly(ethylene glycol) (PEG) nanogel, which possessed lysosome-escape ability based on swelling effect of PEG nanomicelles. Results indicate the probes we proposed can be used for sensitive tracking and long-term visualization of H2S in living cells, and the detection limit of H2S is 0.1 mu M. The multifunctional nanoprobes based on escape lysosome technology by help of swelling effect of polymeric micelles offers an important strategy for detecting bioactive substance in living cells.
作者机构：
[Li, Ziyong; Gao, Xinyu; Guo, Hui; Zhang, Haining; Liu, Yifang] Luoyang Normal Univ, Coll Food & Drug, Coll Chem & Chem Engn, Luoyang Key Lab Organ Funct Mol, Luoyang 471934, Peoples R China.;[Yin, Jun; Ma, Xiaoxie] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Yin, Jun; Ma, Xiaoxie] Cent China Normal Univ, Hubei Int Sci & Technol Cooperat Base Pesticide &, Wuhan 430079, Peoples R China.;[Yin, Jun; Ma, Xiaoxie] Cent China Normal Univ, Int Joint Res Ctr Intelligent Biosensing Technol &, Wuhan 430079, Peoples R China.
通讯机构：
[Hui Guo; Jun Yin] C;College of Food and Drug, College of Chemistry and Chemical Engineering, Luoyang Key Laboratory of Organic Functional Molecules, Luoyang Normal University, Luoyang 471934, China<&wdkj&>College of Chemistry, Key Laboratory of Pesticide and Chemical Biology, Ministry of Education;Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis;International Joint Research Center for Intelligent Biosensing Technology and Health, Central China Normal University, Wuhan 430079, China
摘要：
Three novel dithienylethenes modified by bifluoroboron beta-diketonate fragments have been successfully developed. Upon blue light irradiation, they reached photostationary state within 2-5 s, as well as 100% conversion ratio and photocyclization quantum yield of > 0.70. Such fascinating photochromism were endowed by collaborative role of electron-withdrawing effect of BF2bdk group to reduce HOMO-LUMO electronic gap for the open isomer, together with intramolecular hydrogen bonds and CH-pi interactions favoring antiparallel conformation fixation. Moreover, they displayed specific discrimination and photo-switchable bacterial imaging for S. aureus.(c) 2023 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
作者机构：
[Xiaoxie Ma; Yurou Huang; Weijie Chen; Jia Liu; Sheng Hua Liu; Guang-Fu Yang] Central China Normal University, college of chemistry, CHINA;[Jun Yin] centra china Normal University, college of chemistry, 152 Luoyu Road, 430079 Wuhan, CHINA
通讯机构：
[Jun Yin] c;centra china Normal University, college of chemistry, 152 Luoyu Road, 430079 Wuhan, CHINA
摘要：
The cationic nature of heptamethine cyanines gives them the capacity to form aggregates with salts by electrostatic interactions. In this work, NaCl promoted J‐aggregate formation of aza‐coating heptamethine cyanines is explored. NaCl can induce the N‐benzyloxycarbonyl Cy‐CO2Bz to assemble into a J‐aggregate having an absorption at 890 nm. Its excellent fluorescence response to NaCl implies that it has great potential for use as a probe for tracing salt stress in plants. Moreover, NaCl also promotes formation of J‐aggregates from the N‐ethyloxycarbonyl Cy‐CO2Et. The aggregate shows an intense absorption at 910 nm compared to the monomer which absorbs at 766 nm. Its J‐aggregated form can serve as a photothermal agent. And the photothermal conversion efficiency is increased from 29.37% to 57.59%. This effort leads to the development of two applications of new cyanine J‐aggregates including one for tracing salt stress of plants and the other for promoting photothermal therapy of tumors.
通讯机构：
[Zhiqiang Xu; Sheng Hua Liu; Zhiqiang Xu Zhiqiang Xu Zhiqiang Xu; Sheng Hua Liu Sheng Hua Liu Sheng Hua Liu] K;Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan, Hubei, 430205 China<&wdkj&>Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, Hubei, 430079 China
摘要：
In this paper, we successfully synthesized paramagnetic osmanaphthalenes and diamagnetic osmanaphthalyne derivatives in good yields through Os‐carbyne precursors by visible‐light induced strategy and intramolecular C—H activation under O2 atmosphere, respectively. NMR spectroscopy and X‐ray crystallographic analysis confirmed the structures of these novel fused osmacycles, of which osmabenzo[b]carbazole and its osmabenzyne analogues can be regarded as the first example of fused‐ring osmanaphthalene/osmanaphthalyne complexes, thus enriching the diversity of fused metallaaromatic library. Furthermore, the aromaticity, magnetic, and electronic absorption properties were explored with the aid of experimental study as well as theoretical calculations. Comprehensive Summary Simple and efficient synthesis of metalla‐aromatics with tailored distinctive optical, magnetic, redox, and light‐emitting properties is attractive yet challenging. We have now successfully synthesized three paramagnetic osmanaphthalenes bearing different electron‐donating groups as well as the first osmabenzo[b]carbazole containing an osmanaphthalene unit with four fused rings in good yields by subjecting suitable osmium carbyne precursors to a visible‐light‐induced protocol. In contrast, the four corresponding diamagnetic cyclic metal carbynes containing an osmanaphthalyne unit were obtained from the same osmium carbyne precursors by a heating method. The lowest‐energy absorptions of the paramagnetic osmanaphthalenes are red‐shifted compared to the corresponding osmanaphthalynes. Additionally, experimental observations including X‐ray analysis as well as nucleus‐independent chemical shift (NICS), anisotropy of the current‐induced density (AICD) calculations revealed these novel osmapolycycles bearing phosphonium substituents to be of aromatic character.
作者机构：
[Song, Ke; Chen, Wei-Jie; Yang, Jing-Fang; Yang, Guang-Fu; Yin, Jun; Fu, Yi-Xuan; Pei, Rong-Jie; Zhou, Li-Ming; Hewage, Kamalani Achala H.; He, Bo; Hao, Ge-Fei] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Song, Ke; Chen, Wei-Jie; Yang, Jing-Fang; Yang, Guang-Fu; Yin, Jun; Fu, Yi-Xuan; Pei, Rong-Jie; Zhou, Li-Ming; Hewage, Kamalani Achala H.; He, Bo; Hao, Ge-Fei] Cent China Normal Univ, Int Joint Res Ctr Intelligent Biosensor Technol &, Wuhan 430079, Peoples R China.;[Chen, Mo-Xian] Nanjing Forestry Univ, Coll Biol & Environm, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China.;[Chen, Mo-Xian] Nanjing Forestry Univ, Coll Biol & Environm, Key Lab Natl Forestry & Grassland Adm Subtrop For, Nanjing 210037, Peoples R China.;[Zhang, Jian-Hua] Hong Kong Baptist Univ, Dept Biol, Hong Kong 300072, Peoples R China.
通讯机构：
[Ge-Fei Hao; Guang-Fu Yang] K;Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China<&wdkj&>International Joint Research Center for Intelligent Biosensor Technology and Health, Central China Normal University, Wuhan 430079, China
期刊：
Chemical Engineering Journal,2022年450:138087 ISSN：1385-8947
通讯作者：
Guo, Hui(guohui0319@nankai.edu.cn)
作者机构：
[Li, Ziyong; Guo, Hui; Chen, Si; Wang, Mingxing; Zhou, Hui; Zhang, Haining; Yang, Shuren] Luoyang Normal Univ, Coll Food & Drug, Coll Chem & Chem Engn, Luoyang Key Lab Organ Funct Mol, Luoyang 471934, Peoples R China.;[Huang, Yurou; Yin, Jun] Cent China Normal Univ, Minist Educ,Coll Chem,Hubei Int Sci & Technol Coop, Int Joint Res Ctr Intelligent Biosensing Technol &, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Hui Guo] L;[Jun Yin] K;Luoyang Key Laboratory of Organic Functional Molecules, College of Food and Drug, College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang 471934, PR China<&wdkj&>Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, PR China
