作者机构：
College of Life Science, Central China Normal University, Wuhan 430079, China;Nanyang Technological;Nanyang Technological University, Nanyang Avenue, 639798, Singapore
会议名称：
第十届室内空气品质和质量国际学术会议(Proceedings of the 10th International Conference on Indoor Air Quality and Climate)
会议时间：
2005-09-04
会议地点：
北京
会议论文集名称：
第十届室内空气品质和质量国际学术会议
关键词：
Formaldehyde;Oxidative damage;SOD;GSH;MDA
摘要：
Few researches concern about oxidative damage of inhaled formaldehyde in medium or low concentrations.Therefore, we treated three groups of mice with 1.0mg/m3, 3.0mg/m3 formaldehyde and the control group inhaled fresh air. All of the mice were treated 6 hours per day for 10 days. After exposure, the activities of total superoxide dismutase (SOD), content of glutathione (GSH) and malondialdehyde (MDA) in five organs-brain, heart, lung,liver and kidney were measured. The results showed that inhaled formaldehyde caused the obvious decrease of SOD activities in five organs (P＜0.01) in 3.0mg/m3 group, and the level of GSH in all treated groups were decreased significantly in various organs (P＜0.05) compared with fresh air group. The level of MDA in all organs of 3.0mg/m were increased significantly (p＜0.05) compared with fresh air group. In conclusion, medium concentration formaldehyde gas induces significantly oxidative damage of various organs.
期刊：
INDOOR AIR 2005: PROCEEDINGS OF THE 10TH INTERNATIONAL CONFERENCE ON INDOOR AIR QUALITY AND CLIMATE, VOLS 1-5,2005年15:3805-3809 ISSN：0908-5920
通讯作者：
Yang, X
作者机构：
[Qiao, Y; Lu, ZS; Yan, Y; Li, CM; Yang, X; Qi, QP] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Yang, X] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
10th International Conference on Indoor Air Quality and Climate (Indoor Air 2005)
会议时间：
SEP 04-09, 2005
会议地点：
Beijing, PEOPLES R CHINA
会议主办单位：
Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
关键词：
IAQ;formaldehyde;neurotoxicity;Morris water maze
摘要：
Purpose: To find out if any neurotoxicity effect exists in the mice that exposed to low-level formaldehyde. Approach: After exposed to the gaseous formaldehyde at 0 mg/m3, 1.0 mg/m3 and 3.0 mg/m3 levels, the mice were tested in Morris water maze. Findings: Results demonstrated that there were significant differences on water maze performances between 0 mg/m3 and 3.0 mg/m3 formaldehyde inhaled groups (p<0.01) but little between 0 mg/m3 and 1.0 mg/m3 groups. Conclusions: These findings indicated that the neurotoxicity effect of formaldehyde assuredly existed, and it may occur only at middle-level (3.0 mg/m3) gaseous formaldehyde but not do at low-levels.
期刊：
INDOOR AIR 2005: PROCEEDINGS OF THE 10TH INTERNATIONAL CONFERENCE ON INDOOR AIR QUALITY AND CLIMATE, VOLS 1-5,2005年:2002-2006
通讯作者：
Yan, Y
作者机构：
[Liu, J; Lu, ZS; Yan, Y; Yang, X; Li, R; Wang, GX] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Yan, Y] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
第十届室内空气品质和质量国际学术会议(Proceedings of the 10th International Conference on Indoor Air Quality and Climate)
会议时间：
2005-09-04
会议地点：
北京
会议论文集名称：
第十届室内空气品质和质量国际学术会议
关键词：
Formaldehyde;Wood-based board;Emission model
摘要：
In order to understand the rule of formaldehyde emission from the wood-based boards bought from the construction material markets of China, a formaldehyde emission study was undertaken in our laboratory during July 2001-March 2002. The chamber experiments were carried out with the international standard method and the chamber parameters were controlled to T=23.0 degrees C +/- 0.5 degrees C, RH=45%+/- 5%, ACH=1.0 +/- 0.03 h(-1), Loading factor=1.0 +/- 0.03 m(2)m(-3). By the analysis of formaldehyde emitted from 11 types of wood-based boards taken from construction material markets in Wuhan City of China, the experimental results show that the emission concentrations in the chamber obviously varied along with time. We had tried to use the mathematic exponent model y = y(0) + A(1)e(-(x-x0)/t1), and found which fit the short-term and long-term emission procession with chamber concentrations vs escape time very well.
期刊：
INDOOR AIR 2005: PROCEEDINGS OF THE 10TH INTERNATIONAL CONFERENCE ON INDOOR AIR QUALITY AND CLIMATE, VOLS 1-5,2005年15:3691-3695 ISSN：0908-5920
通讯作者：
Yang, X
作者机构：
[He, HJ; Lu, ZS; Wu, J; Yan, Y; Li, CM; Liu, HL; Yang, X] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Yang, X] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
10th International Conference on Indoor Air Quality and Climate (Indoor Air 2005)
会议时间：
SEP 04-09, 2005
会议地点：
Beijing, PEOPLES R CHINA
会议主办单位：
Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
关键词：
IAQ;formaldehyde;blinking frequency;irritant intensity;substance P
摘要：
To find out reliable biomarkers in China for acute irritation caused by indoor air pollutants, we carried out this study. A controlled human exposure experiment was used as the main research method. Subject eyes were exposed to gaseous formaldehyde at different doses (0 mg/m(3), 1.0 mg/m(3) 2.0 mg/m(3), 3.0 mg/m(3)) five minutes a day for 4 days. The content of substance P in nasal lavage was determined by radio immune assay (RIA), and the blinking frequency and irritant intensity of their eyes were measured at the same time. The results showed that there were dose-dependent effects between blinking frequency and formaldehyde levels (r=0.980, P < 0.05), and so were there between irritant intensity and formaldehyde levels (r=0.985, P < 0.05). The content of substance P in nasal lavage increased after exposures, especially after the exposure of 3mg/m(2) formaldehyde (p < 0.05). This might explain that formaldehyde could stimulate trigeminal nerve ends of the eyes, which would release substance P from its nasal branches through axon reflex.
摘要：
Purpose: Whether formaldehyde induces DNA breakage or not is still in debate. So far it is not clear whether dose-effect between formaldehyde and DNA breakage takes place while the DPC exists. Approach: In this study we had chosen human buccal cells as materials to explore the genotoxicity of liquid and gaseous formaldehyde with comet assay. Findings: The results showed that liquid formaldehyde induced DNA breakage in the range from 5 mu M to 10 mu M (P<0.05, compared with control group), and 7.5 mu M was found to induce DNA breakage most. The DNA crosslinks were significantly found at 15 mu M, 30 mu M and 50 mu M (P<0.01, compared with 7.5 mu M group). DNA breakage caused by gaseous formaldehyde was found at 0.5 mg/m(3) and 1.0 mg/m(3) (P<0.01, compared with control group) but DNA crosslinks; at 3.0 mg/m(3) Significantly. Conclusions: The results suggest that formaldehyde induces DNA breakage in a very low concentration range but induces DNA crosslinks in a higher range.
摘要：
To explore if the NOS level in airway is associated with air formaldehyde. In this study 24 male Kun Ming mice were divided into 4 exposure groups (0 mg/m(3), 0.5 mg/m(3), 1.0 mg/m(3), 3.0 mg/m(3)) for 72h continuously. After exposure the NOS activities and the NO detection rate in lungs of mice were measured with reagent kits. The results showed that along with the formaldehyde ascension the NOS activities were restrained at first and then improved significantly (P<0.05), while the NO detection rate showed the similar regulation. We suggest that formaldehyde play dual effects in vivo: at a low formaldehyde exposure (<= 0.5 mg/m(3)), the NOS activities in lungs is restrained. However, the repeated high formaldehyde exposure would alter the VR1 mRNA expression levels in the lung of mice, then the NOS activities shall be increased and the NO concentration in airway will also be ascended.
摘要：
Formaldehyde, which is well known as a toxic gas and an exogenous compound, is also produced endogenously via cytochrome P450 (CYP) mediated demethylation of compounds containing the CH3N or CH3O functionality. As the similarity to the accepted novel signaling molecules endogenous nitric oxide (NO), carbon monoxide (CO), and hydrogen sulfide (H2S), we hypothesized that endogenous formaldehyde may be another novel signaling molecule. We first compared the three signaling molecules to give the minimal sense criteria for signaling molecule. Formaldehyde fits with all the criteria. We also proposed the possible roles of endogenous formaldehyde in order to give a basis for further study. Formaldehyde may regulate NO level, can influence the structure of macromolecules such as DNA and proteins at molecular level. This hypothesis may start a new direction in the further study on endogenous formaldehyde.
摘要：
目的:探讨超氧化物歧化酶模型化合物(models of superoxide dismutase,MSOD)对离体人肝癌细胞毒作用.方法:采用离体培养的人肝癌细胞SSMC-7721细胞株,常规方法接种入24孔板,加入SOD模型化合物,以彗星实验为终点效应,检测其对肿瘤细胞的影响.结果:通过彗星试验检测,各剂量组所起的肝癌细胞DNA损伤或DNA断裂的程度呈明显的剂量效应关系.结论:这种模型化合物对离体培养的肝癌细胞有明显细胞毒作用.
