作者机构:
[Yun Cao; Mengmeng Liu; Wenying Zhang; Xiaotong Zhang] China CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China;National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China;School of Public Health, Anhui Medical University, Hefei 230032, China;[Mats Tysklind] Department of Chemistry, Umea University, Umea SE-901 87, Sweden;College of Life Science, Huazhong Normal University, Wuhan 430079, China
通讯机构:
[Xianliang Wang] C;China CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China<&wdkj&>National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China<&wdkj&>School of Public Health, Anhui Medical University, Hefei 230032, China
摘要:
To explore the multicenter characteristics of endocrine-like phthalate esters (PAEs) in household dust and propose effective control strategies for global indoor public health. An on-site observational investigation was conducted in nine Chinese cities from 2018 to 2019. A total of 246 household dust samples were collected and analyzed for ten PAE congeners using Gas Chromatography-Mass Spectrometry (GC-MS). Questionnaires were used to gather information on building conditions, indoor behaviors, and ventilation habits. In residential dust from the nine cities, the total concentrations of the ten PAE congeners (∑PAEs) ranged from 0.921 to 29097.297 µg/g. Dicyclohexyl phthalate (DCHP) and di (2-ethylhexyl) phthalate (DEHP) were the dominant congeners in ∑PAEs. Childhood exposure to PAEs through dust ingestion was four orders of magnitude higher than through inhalation, with a carcinogenic risk of 5.47 × 10−6 for DEHP exposure in household dust. Higher ∑PAEs concentrations were associated with higher temperature, double glazing, wall paint usage, television and computer use, and indoor plant growth. This multicenter on-site investigation confirmed PAE pollution characteristics and uncovered the inacceptable risk of daily DEHP exposure in household dust under real living conditions. Effective mitigation measures based on household-related information, residential characteristics, decoration materials, and lifestyle should be taken to build a healthy household environment.
摘要:
This study developed a prediction method to determine the distribution of phthalate esters (PAEs) in indoor dust. A gradient boosting decision tree model (GBRT) was trained by using 267 samples in Shanghai, including PAEs concentrations in indoor dust and data obtained from continuous monitoring, as well as the survey of indoor environment. Environmental exposure, residents' lifestyle, and building characteristics data were collected from 8 cities in China. Based on this, the well -trained GBRT model accurately predicted PAEs concentrations, with goodness of fit (R-2) > 0.94, mean absolute error (MAE) approaching 0, and mean squared error (MSE) approaching 0. This study identified key relationships between input parameters and PAEs concentrations. The average increment of PAEs concentration was greater than 50 % when using more than 2 electronic devices in bedroom. Diisobutyl phthalate (DiBP) concentration increased by approximately 200 % when cleaning frequency was less than once every fortnight. Bis (2-ethylhexyl) phthalate (DEHP) concentration increased by over 43 % when dampness -related exposure indicators exceeding 3, and by up to 74 % with extensive usage of polyvinyl chloride (PVC) floorings. Furthermore, the study found higher PAEs concentrations in southern China compared to northern cities.
摘要:
Globally, approximately 6-20% of women who are of reproductive age suffer from polycystic ovary syndrome (PCOS), with environmental factors believed to be significant contributors. Di-2-ethylhexyl phthalate (DEHP) is known to be an endocrine disruptor, and is also suspected of being associated with the occurrence of PCOS, but in vivo studies to verify this association are lacking. In this study, female SD rats were exposed to DEHP at levels of 0.1, 1.0, and 10mg/kg/d, which are comparable to daily human exposure, to explore its potential role in the development of PCOS. The findings indicated that DEHP exposure reduced ovarian and uterine coefficients, decreased accumulation of primordial follicles, increased the prevalence of atretic and cystic follicles and fibrosis in ovarian tissues, altered serum hormone levels, elevated blood glucose levels and insulin resistance, disrupted the endocrine system and resulted in significant oxidative damage in the ovarian tissues. These results imply that DEHP exposure may cause lesions resembling PCOS to develop. By analyzing the differential expression of the proteome, and using GO and KEGG enrichment analyses, we found they were mainly enriched in the metabolic pathway and in the PPAR signaling pathway. We confirmed that activation of the PPARγ signaling pathway caused by DEHP exposure, is related to the emergence of PCOS-like lesions. This research provides direct in vivo experimental evidence for the association between DEHP exposure and PCOS.
摘要:
Polystyrene microplastics (PS-MP) and dibutyl phthalate (DBP) are plastic pollution derivatives (PPDs) commonly found in the natural environment. To investigate the effects of PPD exposure on the risk of allergic asthma, we established a PPD exposure group in a mouse model. The dose administered for PS-MP was 0.1mg/d and for DBP was 30mg/kg/d, with a 5-week oral administration period. The pathological changes of airway tissue and the increase of oxidative stress and inflammatory response confirmed that PPD aggravated eosinophilic allergic asthma in mice. The mitochondrial morphological changes and metabolomics of mice confirmed that ferrotosis and oxidative stress played key roles in this process. Treatment with 100mg/Kg deferoxamine (DFO) provided significant relief, and metabolomic analysis of lung tissue supported the molecular toxicological. Our findings suggest that the increased levels of reactive oxygen species (ROS) in the lungs lead to Th2-mediated eosinophilic inflammation, characterized by elevated IL-4, IL-5, and eosinophils, and reduced INF-γ levels. This inflammatory response is mediated by the NFκB pathway and exacerbates type I hypersensitivity through increased IL-4 production. In this study, the molecular mechanism by which PPD aggravates asthma in mice was elucidated, which helps to improve the understanding of the health effects of PPD and lays a theoretical foundation for addressing the health risks posed by PPD.
作者机构:
[周敏] Geriatric Department, Chongqing Emergency Medical Center, The Fourth People's Hospital of Chongqing, Chongqing, 400030, China;[郑子光; 游宏宇] Western Investment Construction Co., LDT of CCTEB, Chengdu, 610041, China;[郭淼; 喻伟] School of Civil Engineering, Chongqing University, Chongqing, 400045, China;[杨旭] School of Life Sciences, Central China Normal University, Wuhan, 430079, China
通讯机构:
[Yu, W.] S;School of Civil Engineering, China
摘要:
Building envelope dampness exposure is correlated with children's respiratory and allergic diseases. However, little research has compared the variation in the health impact of dampness exposure across multiple cities from a longitudinal perspective. A cross-sectional survey and a repeated one were conducted in children's residences in six cities: Chongqing, Shanghai, Nanjing, Wuhan, Changsha and Taiyuan, China, in 2010 (Phase I) and 2019 (Phase II). We selected 17,810 preschoolers during Phase I and 26,001 preschoolers during Phase II aged 3–6 years without changing residence since birth in the study. The proportion of residences with building envelope dampness exposure and the prevalence of respiratory and allergic diseases, except allergic rhinitis, in preschoolers significantly declined from Phase I to Phase II. Dampness exposure increased the risk for most childhood respiratory diseases in Phase II, with a 34% greater risk of lifetime-ever asthma in early residences and a 36% greater risk of current eczema in current residences. Most diseases showed a significantly positive exposure-response relationship to the cumulative period of building envelope dampness exposure (p < 0.05). The risk of developing current eczema was approximately 1.35 and 1.73 times higher in children exposed to both early and current dampness in Phase I and Phase II, respectively, than in children who had never been exposed. These findings provide new insights into the respiratory and allergic diseases in Chinese preschoolers because of building envelope dampness exposure over the last decade. The increased risk in Phase II raises concerns about the household's dampness environment.
摘要:
Dibutyl phthalate (DBP), used as a plasticizer, is of wide concern as an environmental pollutant since it has certain immunotoxicity. Although there is growing evidence supporting a link between DBP exposure and allergic airway inflammation, there is less information concerned with whether the ferroptosis pathway is involved in DBP-aggravated allergic asthma in ovalbumin (OVA)-sensitized mice. This study aimed to investigate the role and underlying mechanisms of ferroptosis in DBP-exposed allergic asthmatic mice. Balb/c mice were orally exposed to 40mg/kg(-1) DBP for 28 days, followed by sensitization with OVA and seven consecutive challenges with nebulized OVA. We analyzed airway hyperresponsiveness (AHR), immunoglobulins, inflammation and pulmonary histopathology, to investigate whether DBP exacerbates allergic asthma in OVA-induced mice. We also measured the biomarkers of ferroptosis (Fe(2+), GPX4, PTGS2), proteins related to the ferroptosis pathway (VEGF, IL-33, HMGB1, SLC7A11, ALOX15, PEBP1), and indices of lipid peroxidation (ROS, Lipid ROS, GSH, MDA, 4-HNE), to explore the role of ferroptosis in DBP+OVA mice. Finally, we used ferrostatin-1 (Fer-1) as an antagonist against the harmful effects of DBP. The results showed that, DBP+OVA mice had a significant increase in AHR, airway wall remodeling and airway inflammation. Further, we showed that DBP aggravated allergic asthma via ferroptosis and lipid peroxidation, and that Fer-1 inhibited ferroptosis and alleviated the pulmonary toxicity of DBP. These results suggest that ferroptosis participates in the exacerbation of allergic asthma resulting from oral exposure to DBP, highlighting a novel pathway for the connection between DBP and allergic asthma.
摘要:
Engleromyces goetzei Henn (EgH) is a natural fungus that has been used as a traditional edible and medicine for long time in Southwest China. Our study found EgH aqueous extract (EgH‐AE) has very strong activities on antioxidant and anti‐inflammation. At the same time, we also found EgH‐AE has good biocompatibility and cell protective function, so it is biosafe, EgH‐AE may have the prospect of developing into functional beverage. Abstract High body mass index (high BMI, obesity) is a serious public health problem, and “obesity‐induced oxidative stress, inflammation, and cancer” have become modern epidemic diseases. We carried out this study to explore a functional beverage that may protect against obesity‐induced diseases. The Engleromyces goetzei Henn herbal tea is such a candidate. For this study, we carried out LC–MS analysis of E. goetzei Henn aqueous extract (EgH‐AE); then used the Caco‐2 cell line for the model cells and treated the cells with t‐BHP to form an oxidative stress system. An MTT assay was used for testing the biocompatibility and cytoprotective effects; reactive oxygen species and malondialdehyde determination was used for evaluating the antioxidative stress effect; TNF‐α and IL‐1β were used for observing the anti‐inflammatory effect, and 8‐OHdG for monitoring anticancer activity. The results of this study demonstrate that the EgH‐AE has very good biocompatibility with the Caco‐2 cell line and has good cytoprotective, antioxidant, anti‐inflammatory, and anticancer properties. It is clear that EgH‐AE, a kind of ancient herbal tea, may be used to develop a functional beverage that can be given to people with a high BMI to protect against obesity‐induced diseases.
摘要:
<jats:p> This toxicology study was conducted to assess the impact of formaldehyde, a common air pollutant found in Chinese gymnasiums, on the brain function of athletes. In this research, a total of 24 Balb/c male mice of SPF-grade were divided into four groups, each consisting of six mice. The mice were exposed to formaldehyde at different concentrations, including 0 mg/m<jats:sup>3</jats:sup>, 0.5mg/m<jats:sup>3</jats:sup>, 3.0mg/m<jats:sup>3</jats:sup>, and 3.0mg/m<jats:sup>3</jats:sup> in combination with an injection of L-NMMA (N<jats:sup>G</jats:sup>-monomethyl-L-arginine), which is a nitric oxide synthase antagonist. Following a one-week test period (8h per day, over 7days), measurements of biomarkers related to the nitric oxide (NO)/cGMP-cAMP signaling pathway were carried out on the experimental animals post-treatment. The study found that: (1) Exposure to formaldehyde can lead to brain cell apoptosis and neurotoxicity; (2) Additionally, formaldehyde exposure was found to alter the biomarkers of the NO/cGMP-cAMP signaling pathway, with some changes being statistically significant ( p < 0.05 or p < 0.01); (3) The use of L-NMMA, an antagonist of the NO/cGMP-cAMP signaling pathway, was found to prevent these biomarker changes and had a protective effect on brain cells. The study suggests that the negative impact of formaldehyde on the brain function of mice is linked to the regulation of the NO/cGMP-cAMP signaling pathway. </jats:p>
通讯机构:
[Zeng, Y.] B;[Li, J.] J;Brain Science and Advanced Technology Institute, School of Medicine, Wuhan University of Science and Technology, Wuhan 430081, China
作者机构:
[Xu, Dongqun; Liu, Jingyi; Li, Yunpu; Li, Na; Chang, Junrui; Wang, Qin; Han, Jingxiu; Hao, Shuxin; Xu, Chunyu; Xu, Donggang; Liu, Yue; Meng, Congshen] China CDC Key Laboratory of Environment and Population Health, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing, China;[Xing, Weiwei; Fu, Wenliang; Xu, Dongqun; Xia, Wenrong] Academy of Military Medical Sciences, Academy of Military Sciences, Beijing, China;[Chen, Mingqing; Yang, Xu] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Hubei Province, Wuhan City, China
通讯机构:
[Xu, D.] C;China CDC Key Laboratory of Environment and Population Health, China
摘要:
The changing climate is one of the most important factors affecting public health. Older people are particularly threatened due to their less efficient immune systems. To evaluate the potential benefits of short-term indoor dehumidification on their circulation and cardiopulmonary health, we conducted a random, cross-over experiment with 36 healthy residents of an aged-care center in Chongqing, China in 2020. Vapor compression dehumidifiers were used over two 48-h periods. At the end of each 48h, we immediately measured sixteen circulatory system biomarkers of inflammation, coagulation, and oxidative stress; lung function; blood pressure; and heart rate. Indoor temperature and relative humidity were monitored throughout the study period. Linear, mixed-effect models were used to associate health endpoints with indoor relative humidity. This intervention study showed that when the indoor relative humidity decreased from 75% to 45%: (1) the coagulation indicators, sCD40l, and PAI-1, decreased significantly, by 58.82% and 23.50%, respectively; (2) the inflammatory indicators, CRP, IL-6, and TNF-α decreased significantly, by 4.09%, 25.78%, and 10.60%, respectively; (3) PEF, FEV(1) and FVC were increased significantly by 20.08%, 14.54%, and 15.75% respectively. To the best of our knowledge, this is the first study to examine the impact of short-term dehumidification on clinical and biochemical measures of cardiorespiratory health in humid areas, and our study suggests that RH in the dehumidified rooms (46.9±8.7%) may be healthier than that in humid rooms (75.2±7.9%). Humidity may be involved in the development of atherosclerosis by activating oxidative stress and mediating the secretion of inflammatory indicators. At the same time, platelet activation induced by oxidative stress stimulates thrombosis to increase cardiovascular risk in older people. Conclusion: This intervention study shows that in a Chinese city like Chongqing with serious indoor environmental humidity, indoor short-term dehumidification has obvious cardiopulmonary benefits for the healthy elderly.