作者机构：
[Zhou, Yanping; Song, Dandan; Cui, Yanfang; Xu, Shan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.;[Hu, Sheng; Tang, Jing; Zhu, Xianmin] Hubei Prov Tumor Hosp, Dept Med Oncol, Wuhan 430079, Hubei, Peoples R China.;[Geng, Hui] Cent China Normal Univ, Dept Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Xu, Shan] Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China.;[Xu, Shan] Univ Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan,CAS Key Lab Magne, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China.
通讯机构：
[Cui, Yanfang] C;[Hu, Sheng] H;[Cui, Yanfang] M;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.;Hubei Prov Tumor Hosp, Dept Med Oncol, Wuhan 430079, Hubei, Peoples R China.
摘要：
Most lung cancers are diagnosed at fairly advanced stages due to limited clinical symptoms. Platinum-based chemotherapy, either as single regimen or in combination with radiation, is one of the major recommendations for the patients. Earlier evaluation of the effectiveness of the chemotherapies is critical for developing better treatment plan given the toxicity of the chemotherapeutic reagents. Drug efficacy could be reflected in the systemic metabolism characteristics though knowledge about which remains scarce. In this study, serum metabolism influence of three types of commonly used platinum-based combination chemotherapy regimens, namely cisplatin with gemcitabine, vinorelbine or docetaxel, were studied using pattern recognition coupled with nuclear magnetic resonance techniques. The treated patients were divided into sensitive or insensitive subgroups according to their response to the treatments. We found that insensitive subjects can be identified from the sensitive ones with up-regulation of glucose and taurine but reduced alanine and lactate concentrations in serum. The combination chemotherapy of lung cancer is accompanied by disturbances of multiple metabolic pathways such as energy metabolism, phosphatidylcholine biosynthesis, so that the treated patients were marginally discriminated from the untreated. Serum metabolic profile of patients shows potential as an indicator of their response to platinum-based combination chemotherapy.
摘要：
目的先前的研究发现,在行为相关的CF-FM声刺激下,CF-FM蝙蝠的下丘(inferior colliculus,IC)神经元显示出要么产生单反应(single-on,SO),要么产生双反应(double-on,DO)(Fu et al.,2010;Tang et al.,2011;Li et al.,2015)。两类神经元对行为相关声信号的频率选择性是否不同?尚需研究。方法 10只普氏蹄蝠(Hi
作者机构：
[Fu, Zi-Ying; Chen, Qi-Cai; Tang, Jia; Mei, Hui-Xian; Cheng, Liang] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.;[Fu, Zi-Ying; Chen, Qi-Cai; Tang, Jia; Mei, Hui-Xian; Cheng, Liang] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Mei, Hui-Xian] Hubei Univ, Sch Sport, Wuhan 430062, Peoples R China.;[Cheng, Liang] CCNU, Minist Educ, Key Lab Adolescent Cyberpsychol & Behav, Wuhan 430079, Peoples R China.;[Cheng, Liang] Cent China Normal Univ, Sch Psychol, Wuhan 430079, Peoples R China.
通讯机构：
[Chen, Qi-Cai] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
作者：
Kang Peng;Zi-Ying Fu（付子英）;Jia Tang;Ming-Jian Yang;Jing Wang;...
作者机构：
[Zi-Ying Fu; Kang Peng; Jia Tang; Ming-Jian Yang; Jing Wang; Qi-Cai Chen] School of Life Sciences,Central China Normal University
会议名称：
2016年国际生理学学术大会
会议时间：
2016-09-25
会议地点：
中国北京
会议论文集名称：
2016年国际生理学学术大会论文集
关键词：
behavior-related sound signal;auditory midbrain;CF-FM bat
摘要：
The CF-FM signal emitted by CF-FM bats may be called "a simple-complex sound signal",which CF and FM components have different function during echolocation（O’Neill & Suga,1982; Kober et al.,1990; Fu et al.,2010; Schnitzler & Denzinger,2011; Jakobsen et al.,2013）.How to process the complex and behavior-related sound signals in the auditory system is known very little up to now.Therefore,the study on the cellular basis and mechanism of the CF-FM sound signal processing in the auditory system have the very important significance for our further understanding complex sound signal processing.Because CF-FM signal used in the study simulates the echolocation call of the CF-FM bat（Hipposideros pratti）,it is thought to be a behavior-related sound signal.We investigated the CF-FM sound signal processing in auditory midbrains（i.e.inferior colliculus,IC） of the CF-FM bat（Hipposideros pratti）,FM bat（Pipistrellus abramus） and mouse（Mus musculus,Km） using the intracellular,extracellular and auditory evoked potential recordings and compared the modes of CF-FM signal processing in three animal species.Total 145 sound-sensitive neurons were isolated from the ICs of 7 CF-FM bats using extracellular recording.According to their response patterns to CF-FM stimulus,the recorded neurons could be classified into two types,i.e.single-on（SO）（56%,N=102/182） and double-on（DO） responders（44%,N=80/182）.In addition,178 neurons were intracellularly isolated from the IC of 6 CF-FM bats and 130 among the neurons were examined with CF-FM sounds.67.7%（88/130） of the neurons were SO neurons and the remains（32.3%,42/130） were DO neurons.In addition,we also did the comparative study on the response patterns to CF-FM stimulus in CF-FM bats,FM bats and mice.These results showed that the behavior-related SO and DO response patterns to CF-FM stimulus have formed in the auditory centers of the CF-FM bats and these two response patterns appeared the species-specificity in three animal species.
摘要：
Previous studies of sexual dimorphism in the echolocation pulses of the constant frequency-frequency modulating (CF-FM) bat have been mainly concentrated on the difference in the frequency of the CF component of the predominant second harmonic while neglected other pulse parameters. However, recent studies have shown that other pulse parameters of the predominant second harmonic are also biologically significant to the bat hunting. To complement and advance these studies, we have examined sexual dimorphism of multiple parameters (e.g., duration, frequency, bandwidth of the FM component, and repetition rate of emitted pulses) of the echolocation pulses of the CF-FM bat, Hipposideros pratti. Our studies of the predominant second harmonic show that on average the male bat has higher frequency of the CF component, wider FM bandwidth, and higher pulse repetition rate while the female bat has longer duration of the CF and FM components. These observations suggest that bats may potentially use this sexual dimorphism in echolocation pulse parameters for social communication and species and sex identification.
通讯机构：
[Tang, Jiaqi] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Sect Environm Biomed, Wuhan, Peoples R China.
摘要：
Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer commonly used in PVC that may leach into the environment, and has been shown to adversely affect the health of humans and animals. We undertook a study to ascertain the neurotoxicity of DEHP in Kunming mice. This study included three rounds of testing. In the first round, Kunming mice were exposed to different concentrations of DEHP (0, 5, 50, 500 mg kg−1 per day) after which their cognitive ability was assessed using the Morris water maze (MWM) test. The reactive oxygen species (ROS) content in tissue and the malondialdehyde (MDA) content of brains were also measured. In the second round, vitamin E (50 mg kg−1 per day) was given daily as an anti-oxidant via the intragastric route. Cognitive deficits and locomotor activity, as well as ROS and MDA contents were tested employing the same methods. In the third round, the depressive mood of mice after DEHP exposure (500 mg kg−1 per day) was measured using the open field test, the tail suspension test, and the forced swim test. The main findings of this study include: (1) a statistical association exists between DEHP oral exposure and spatial learning (DEHP 500 mg kg−1 per day) and memory (DEHP 50 mg kg−1 per day) dysfunction as ascertained by an MWM test of Kunming mice. (2) A statistical association was also found between DEHP oral exposure (50 and 500 mg kg−1 per day) and oxidative stress (ROS and MDA) of mouse brain tissue. (3) Co-administration of vitamin E (50 mg kg−1 per day) diminishes the elevation of ROS and MDA induced by DEHP (50 mg kg−1 per day) from significant levels to non-significant levels. (4) Co-administration of vitamin E (50 mg kg−1 per day) protects against mouse memory dysfunction induced by DEHP (50 mg kg−1 per day) from being significant to being not significant. (5) In the 5 mg kg−1 per day DEHP exposure groups, oxidative stress in brain tissue, and neurobehavioral changes were not found. (6) High dose DEHP exposure (500 mg kg−1 per day) may induce behavioral despair in mice. Conclusions: These data suggest that DEHP is neurotoxic with regard to cognitive ability and locomotor activity.
Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer commonly used in PVC that may leach into the environment, and has been shown to adversely affect the health of humans and animals.
作者机构：
[Fu, Z. -Y.; Li, Y. -L.; Chen, Q. -C.; Tang, J.; Wang, J.; Peng, K.; Yang, M. -J.] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Fu, Z. -Y.; Li, Y. -L.; Chen, Q. -C.; Tang, J.; Wang, J.; Peng, K.; Yang, M. -J.] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Yang, L. -J.] Cent China Normal Univ, Inst Biophys, Wuhan 430079, Hubei, Peoples R China.;[Yang, L. -J.] Cent China Normal Univ, Dept Phys, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Chen, Q. -C.] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
摘要：
To probe the mechanism underlying the auditory behavior-related response patterns of inferior collicular neurons to constant frequency-frequency modulation (CF-FM) stimulus in Hipposideros pratti, we studied the role of post-spike hyperpolarization (PSH) in the formation of response patterns. Neurons obtained by in vivo extracellular (N = 145) and intracellular (N = 171) recordings could be consistently classified into single-on (SO) and double-on (DO) neurons. Using intracellular recording, we found that both SO and DO neurons have a PSH with different durations. Statistical analysis showed that most SO neurons had a longer PSH duration than DO neurons (p < 0.01). These data suggested that the PSH directly participated in the formation of SO and DO neurons, and the PSH elicited by the CF component was the main synaptic mechanism underlying the SO and DO response patterns. The possible biological significance of these findings relevant to bat echolocation is discussed. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.
摘要：
众所周知,"同频掩蔽大于异频掩蔽、高强度掩蔽大于低强度掩蔽",抗听觉前掩蔽的能力强意味着抗干扰能力强.先前的研究(Covey et a1.,1996；Backoff et al.,1997；Frisina,2001；Wehr&Zador,2005；Voytenko&Galazyuk,2010；付子英等,2013)表明,听觉中枢神经元的前掩蔽效应与神经抑制有关,并推测由掩蔽声激活的抑制性输入与探测声激活的兴奋性输入或/和抑制性输入,经过在突触部位相互作用和整合,产生前掩蔽.
摘要：
<正>频率分析是听觉系统的最基本的功能之一。先前的研究发现,在外周和较低位的中枢听觉结构内神经元的频率调谐曲线(FTC)的带宽均比较宽,而在进入较高位的中枢后,FTC变的越来越锐化(sharpness),频率分析也越来越精确(Suga,1995;Phillips et al.,1988),这提示听中枢有锐化神经元FT的能力和机制。后来的研究者沿用双声刺激方法在猫的听皮质(AC)(Sutter et
作者机构：
[Fu, Zi-Ying; Chen, Qi-Cai; Tang, Jia; Xu, Na; Wang, Jing] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Jen, Philip Hung-Sun] Bingzhou Med Coll, Coll Special Educ, Yantai, Shandong, Peoples R China.;[Jen, Philip Hung-Sun] Natl Taiwan Normal Univ, Dept Life Sci, Taipei, Taiwan.;[Jen, Philip Hung-Sun] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA.
通讯机构：
[Chen, Qi-Cai] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
关键词：
Single-on neuron;Double-on neuron;Firing rates;Response latency;Leaf-nosed bat
摘要：
Previous studies show that when stimulated with constant frequency-frequency modulated (CF-FM) sounds, the inferior collicular neurons of the leaf-nosed bat, Hipposideros armiger, either discharge impulses only to the CF component (single-on, SO neurons) or to both CF and FM components (double-on, DO neurons). In this study, we specifically determine the role of the FM component in shaping the number of impulses and response latency of these two types of neurons in response to CF-FM sounds. Adding the FM component to the CF sounds significantly decreases the number of impulses of both SO and DO neurons but shortens the response latency of DO neurons in response to the CF component of the CF-FM sounds. The possible neural mechanisms underlying these seemingly paradoxical observations are briefly discussed based on our preliminary intracellular recording studies. Biological relevance of these findings in relation to different phases of bats' hunting is also discussed. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
作者机构：
[付子英; 徐娜; 李娟; 唐佳; 陈其才; 石青] College of Life Sciences and Hubei Key Lab of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, 430079, China
通讯机构：
[Fu, Z.] C;College of Life Sciences and Hubei Key Lab of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, China
作者机构：
[Tang Jia] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Tang Jia] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.
关键词：
多普勒频移补偿;谐波;发放模式;强度-潜伏期函数;普氏蹄蝠
摘要：
为了探讨普氏蹄蝠下丘神经元在处理多普勒频移补偿后回声定位信号中的作用，实验采用双声刺激模式模拟蝙蝠不同飞行状态下产生多普勒频移补偿后的脉冲-回声对，即发声频率改变，回声频率维持恒定的情况下，研究下丘神经元对不同补偿值下的回声反应恢复率．结果发现：根据神经元在某一补偿值下对回声信号反应的恢复率是否超过70%，可将其分为具有选择性（S）和无选择性（NS）的两类神经元．且谐波内S神经元所占比例（68%）远超过非谐波内S神经元（39%）．分析神经元的发放模式发现谐波内S神经元中相位型发放模式比例（44.3%）明显高于其他三种类型神经元．另外，虽然S和NS神经元的强度-潜伏期函数类型均以饱和型为主，但谐波内S神经元强度-潜伏期函数的最佳强度（best amplitude，BA）（95.3±14.0） dB SPL低于NS神经元的BA（104.1±10.2） dB SPL（P 〈 0.01），同时也低于非谐波内S神经元的BA（109.7±7.9） dB SPL（P 〈 0.01）．以上实验结果表明，在下丘水平，神经元就已对多普勒频移补偿后回声定位信号的处理有了分工，集中在谐波内的S神经元通过提高对某一补偿值下回声信号反应的恢复率实现，对回声信息的精确编码，避免其他杂波干扰信息．同时，谐波内S神经元的发放模式和强度-潜伏期函数特点也满足其在复杂环境中精确声学成像的需求．
作者机构：
[付子英; 杨明建; 李玉兰; 唐佳; 彭康; 王靖; 陈其才] School of Life Sciences and Hubei Key Lab of Genetic Regulation and Integration Biology, Central China Normal University, Wuhan, 430079, China
通讯机构：
[Chen, Q.] S;School of Life Sciences and Hubei Key Lab of Genetic Regulation and Integration Biology, Central China Normal University, Wuhan, China
摘要：
The discharge patterns of neurons in auditory centers encode information about sounds. However, few studies have focused on the synaptic mechanisms underlying the shaping of discharge patterns using intracellular recording techniques. Here, we investigated the discharge patterns of inferior collicular (IC) neurons using intracellular recordings to further elucidate the mechanisms underlying the shaping of discharge patterns. Under in vivo intracellular recording conditions, recordings were obtained from 66 IC neurons in 18 healthy adult mice (Mus musculus, Km) under free fi eld-stimulation. Fiftyeight of these neurons fi red bursts of action potentials (APs) to auditory stimuli and the remaining eight just generated local responses such as excitatory (n = 4) or inhibitory (n = 4) postsynaptic potentials. Based on the APs and subthreshold responses, the discharge patterns were classifi ed into seven types: phasic (24/58, 41.4%), phasic burst (8/58,13.8%), pauser (4/58, 6.9%), phasic-pauser (1/58, 1.7%), chopper (2/58, 3.4%), primary-like tonic (14/58, 24.1%) and sound-induced inhibitory (5/58,8.6%). We concluded that (1) IC neurons exhibit at least seven distinct discharge patterns; (2) inhibition participates in shaping the discharge pattern of most IC neurons and plays a role in sculpting the pattern, except for the primary-like tonic pattern which was not shaped by inhibition; and (3) local neural circuits are the likely structural basis that shapes the discharge patterns of IC neurons and can be formed either in the IC or in lower-level auditory structures.
