摘要：
Native ethylene‐producing cyanobacterium Nostoc sphaeroides is hybridized with indium phosphide) nanomaterial via a self‐assembly strategy, and the resulting biohybrid system achieved high photosynthetic ethylene production. Advanced analytical techniques revealed that an InP‐modulated photosystem I activity underlined the augmented ethylene production of the biohybrid cyanobacterium. Abstract Biomanufacturing of ethylene is particularly important for modern society. Cyanobacterial cells are able to photosynthesize various valuable chemicals. A promising platform for next‐generation biomanufacturing, the semiconductor‐cyanobacterial hybrid systems are capable of enhancing the solar‐to‐chemical conversion efficiency. Herein, the native ethylene‐producing capability of a filamentous cyanobacterium Nostoc sphaeroides is confirmed experimentally. The self‐assembly characteristic of N. sphaeroides is exploited to facilitate its interaction with InP nanomaterial, and the resulting biohybrid system gave rise to further elevated photosynthetic ethylene production. Based on chlorophyll fluorescence measurement and metabolic analysis, the InP nanomaterial‐augmented photosystem I activity and enhanced ethylene production metabolism of biohybrid cells are confirmed, the mechanism underlying the material‐cell energy transduction as well as nanomaterial‐modulated photosynthetic light and dark reactions are established. This work not only demonstrates the potential application of semiconductor‐N. sphaeroides biohybrid system as a good platform for sustainable ethylene production but also provides an important reference for future studies to construct and optimize nano‐cell biohybrid systems for efficient solar‐driven valuable chemical production.
通讯机构：
[Xie, GS ] H;[Zhang, ZF ] C;Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China.;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
de novo fatty acid synthesis;fibrillin 7;galactolipids;KAS I;plastoglobule;rice
摘要：
Plastoglobules (PGs) contiguous with the outer leaflets of thylakoid membranes regulate lipid metabolism, plastid developmental transitions, and responses to environmental stimuli. However, the function of OsFBN7, a PG-core fibrillin gene in rice, has not been elucidated. Using molecular genetics and physiobiochemical approaches, we observed that OsFBN7 overexpression promoted PG clustering in rice chloroplasts. OsFBN7 interacted with two KAS I enzymes, namely OsKAS Ia and OsKAS Ib, in rice chloroplasts. Lipidomic analysis of chloroplast subcompartments, including PGs in the OsFBN7 overexpression lines, confirmed that levels of diacylglycerol (DAG), a chloroplast lipid precursor and monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), the main chloroplast membrane lipids, were increased in PGs and chloroplasts. Furthermore, OsFBN7 enhanced the abundances of OsKAS Ia/Ib in planta and their stability under oxidative and heat stresses. In addition, RNA sequencing and real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analyses showed that the expression of the DAG synthetase gene PAP1 and MGDG synthase gene MDG2 was upregulated by OsFBN7. In conclusion, this study proposes a new model in which OsFBN7 binds to OsKAS Ia/Ib in chloroplast and enhances their abundance and stability, thereby regulating the chloroplast and PG membrane lipids involved in the formation of PG clusters.
作者机构：
[Mao, Bin; Chen, Meng-Yan; Wang, Qian; Shen, Wei; Li, Chao; Wang, Yu-Feng; Zheng, Ya; Duan, Xin] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, YF ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Ocnus;Drosophila melanogaster;Proteomics;Phosphoproteomics;Testis development
摘要：
Testis is the only organ supporting sperm production and with the largest number of proteins and tissue-specific proteins in animals. In our previous studies, we have found that knockdown of ocnus (ocn), a testis-specific gene, resulted in much smaller testis with no germ cells in Drosophila melanogaster. However, the molecular consequences of ocn knockdown in fly testes are unknown. In this study, through iTRAQ quantitative proteomics sequencing, 606 proteins were identified from fly abdomens as having a significant and at least a 1.5-fold change in expression after ocn knockdown in fly testes, of which 85 were up-regulated and 521 were down-regulated. Among the differential expressed proteins (DEPs), apart from those proteins involved in spermatogenesis, the others extensively affected biological processes of generation of precursor metabolites and energy, metabolic process, and mitochondrial transport. Protein-protein interaction (PPI) analyses of DEPs showed that several kinases and/or phosphatases interacted with Ocn. Re-analyses of the transcriptome revealed 150 differential expressed genes (DEGs) appeared in the DEPs, and their changing trends in expressions after ocn knockdown were consistent. Many common down-regulated DEGs and DEPs were testis-specific or highly expressed in the testis of D. melanogaster. Quantitative RT-PCR (qRT-PCR) confirmed 12 genes appeared in both DEGs and DEPs were significantly down-regulated after ocn knockdown in fly testes. Furthermore, 153 differentially expressed phosphoproteins (DEPPs), including 72 up-regulated and 94 down-regulated phosphorylated proteins were also identified (13 phosphoproteins appeared in both up- and down-regulated groups due to having multiple phosphorylation sites). In addition to those DEPPs associated with spermatogenesis, the other DEPPs were enriched in actin filament-based process, protein folding, and mesoderm development. Some DEPs and DEPPs were involved in Notch, JAK/STAT, and cell death pathways. Given the drastic effect of the ocn knockdown on tissue development and testis cells composition, the differences in protein abundance in the ocn knockdown flies might not necessarily be the direct result of differential gene regulation due to the inactivation of ocn. Nevertheless, our results suggest that the expression of ocn is essential for Drosophila testis development and that its down-regulation disturbs key signaling pathways related to cell survival and differentiation. These DEPs and DEPPs identified may provide significant candidate set for future studies on the mechanism of male reproduction of animals, including humans.
摘要：
Brassinosteroids (BRs) play critical roles in plant growth and development and regulate many important agronomic traits. However, the functions of BRs in strawberry are unclear. This study identified two mutants, named P6 and R87, in woodland strawberry (Fragaria vesca) from EMS mutagenesis populations that exhibit narrow leaves, petals and sepals. Mapping by sequencing and genetic studies revealed that the F. vesca CYP734A129, encoding a putative BR catabolic enzyme, is the causative gene for both P6 and R87. Overexpression of CYP734A129 in both F. vesca and Arabidopsis causes a severe dwarf phenotype, and the BRI1-EMS-SUPPRESSOR 1 (BES1) protein is less abundant in the CYP734A129-overexpressing Arabidopsis seedlings. This suggests that CYP734A129 is functionally conserved with CYP734A1, as a BR-inactivating enzyme. Transcriptome analysis of young leaves revealed that four BR biosynthetic genes were significantly downregulated in P6 (cyp734a129), and photosynthesis-related genes were highly enriched among the up-regulated genes in P6 compared to the wild type. This further supports that CYP734A129 inactivates BRs in F. vesca. Furthermore, we showed that mutations in CYP734A129 do not affect fruit shape and color during ripening in strawberry. Overall, our results suggest that F. vesca CYP734A129 is a BR catabolic enzyme, and provide insights into the roles of CYP734A129 in strawberry.
通讯作者：
Hong Yang<&wdkj&>Hong Yang Hong Yang Hong Yang
作者机构：
[Yang, Hong; Li, Xin-Yu; Mei, Cheng; Wulamu, Dilinuer] Cent China Normal Univ, Inst Entomol, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhan, Shuai; Luo, Xing-Yu; Huang, Yong-Ping] Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, CAS Key Lab Insect Dev & Evolutionary Biol, Inst Plant Physiol & Ecol,Shanghai Inst Biol Sci, Shanghai, Peoples R China.
通讯机构：
[Hong Yang; Hong Yang Hong Yang Hong Yang] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Institute of Entomology, School of Life Sciences, Central China Normal University, Wuhan, China
关键词：
bacterial isolates;germ-free BSF larvae;growth and development;Hermetia illucens;promotive effects
摘要：
Graphical Abstract Analysis of the intestinal bacterial community composition of Hermetia illucens (black soldier fly, BSF) larvae (BSFL) with pyrosequencing showed that the dynamics of bacterial community composition among different larval instars were striking at the genus level. Klebsiella, Clostridium, Providencia, Dysgonomonas, Dysgonomonas, and Providencia were the relatively most abundant bacterial groups in the 1st‐ to 6th‐instar BSFL, respectively. Providencia, Citrobacter, Klebsiella, Ochrobactrum, and Dysgonomonas promoted the growth and development of BSF significantly compared with the germ‐free control by increasing the weight gain of larvae and pupae, as well as the prepupae rate and eclosion rate. In addition, Citrobacter, Klebsiella, and Providencia could shorten the life cycle of BSF significantly. Abstract Black soldier fly (BSF), Hermetia illucens (Diptera: Stratiomyidae), is a prominent insect for the bioconversion of various organic wastes. As a saprotrophic insect, the BSF inhabits microbe‐rich environments. However, the influences of the intestinal microorganisms on BSF growth and development are not very clear. In this study, the dynamics of the intestinal bacterial community of BSF larvae (BSFL) were analyzed using pyrosequencing. Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria were the most prevalent bacterial phyla in the intestines of all larval instars. The dynamic changes in bacterial community compositions among different larval instars were striking at the genus level. Klebsiella, Clostridium, Providencia, and Dysgonomonas were the relatively most abundant bacteria in the 1st‐ to 4th‐instar BSFL, respectively. Dysgonomonas and Providencia also dominated the 5th‐ and 6th‐instar larvae, at ratios of 31.1% and 47.2%, respectively. In total, 148 bacterial strains affiliated with 20 genera were isolated on different media under aerobic and anaerobic conditions. Among them, 6 bacteria, BSF1–BSF6, were selected for further study. The inoculation of the 6 isolates independently into germ‐free BSFL feeding on an artificial diet showed that all the bacteria, except BSF4, significantly promoted BSF growth and development compared with the germ‐free control. Citrobacter, Dysgonomonas, Klebsiella, Ochrobactrum, and Providencia promoted BSF development significantly by increasing the weight gains of larvae and pupae, as well as increasing the prepupae and eclosion rates. In addition, Citrobacter, Klebsiella and Providencia shortened the BSF life cycle significantly. The results illustrate the promotive effects of intestinal bacteria on BSF growth and development.
通讯作者：
Chuan YAN<&wdkj&>Chuan YAN Chuan YAN Chuan YAN
作者机构：
College of Ecology and Environmental Science, Yunnan University, Kunming, China;Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology and Institute of Biodiversity, Yunnan University, Kunming, China;[Hongmao ZHANG; Hongmao ZHANG Hongmao ZHANG Hongmao ZHANG] Institute of Evolution and Ecology, College of Life Sciences, Central China Normal University, Wuhan, China;[Rui LIU; Yongjun ZHANG; Chuan YAN; Rui LIU Rui LIU Rui LIU; Yongjun ZHANG Yongjun ZHANG Yongjun ZHANG; Chuan YAN Chuan YAN Chuan YAN] State Key Laboratory of Grassland Agro-ecosystems, College of Ecology & School of Life Sciences, Lanzhou University, Lanzhou, China;[Lin CAO; Lin CAO Lin CAO Lin CAO] College of Ecology and Environmental Science, Yunnan University, Kunming, China<&wdkj&>Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology and Institute of Biodiversity, Yunnan University, Kunming, China
通讯机构：
[Chuan YAN; Chuan YAN Chuan YAN Chuan YAN] S;State Key Laboratory of Grassland Agro-ecosystems, College of Ecology & School of Life Sciences, Lanzhou University, Lanzhou, China
关键词：
density dependence;mast;resource pulse;rodent;seed
摘要：
Our study provides a global pattern of the associations between seed falls and rodent population dynamics in forests, and density dependence contributed much more to rodent dynamics than seed falls. Abstract One classic system of pulsed resource and animal population is mast seeding and population dynamics of seed‐eating rodents in forests. However, we still lack an understanding of the global patterns regarding the contributions of seed falls to rodent outbreaks or population dynamics. We analyzed a global dataset of coupled long‐term time series of seed abundances and rodent populations from published literature, including 66 and 89 time series (156 rodent–seed pairs from 37 studies) for rodent and seed abundances, respectively. We found only half of the examined rodent populations showed statistically significant coincidence between rodent outbreak and mast‐seeding years. Over all the coupled time series, seed abundance was found to positively correlate with rodent abundance with a one‐year lag, and the relative importance of seed abundance was much lower than that of density dependence in affecting rodent population growth rates. We also found the relative importance of seed abundance decreased, but that of rodent density dependence increased with the latitude of study. For the first time, our work provides a global pattern on the associations between seed falls and rodent population dynamics mostly in mid‐ and high‐latitude forests, and highlights the necessity of more long‐term studies on this subject in more forest ecosystems.
期刊：
Pesticide Biochemistry and Physiology,2023年194:105516 ISSN：0048-3575
通讯作者：
Yang, Yongbo;Liu, KY
作者机构：
[Xia, Zhichao; Liu, Kaiyu; Wei, Wei; Wang, Haixia; Yang, Yongbo; Pan, Shuang; Liu, Leilei] Cent China Normal Univ, Sch Life Sci, Wuhan 430070, Peoples R China.;[Wei, Wei; Liu, Leilei] Wuhan Univ Bioengn, Appl Biotechnol Ctr, Wuhan 430415, Peoples R China.;[Wang, Ling] Inst Hubei Agr Acad, Wuhan 430070, Peoples R China.;[Xiao, Yutao] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China.;[Bravo, Alejandra] Univ Nacl Autonoma Mexico, Inst Biotecnol, Apdo Postal 510-3, Cuernavaca 62250, Morelos, Mexico.
通讯机构：
[Yang, YB; Liu, KY ] C;Cent China Normal Univ, Inst Entomol, Sch Life Sci, Wuhan 430070, Peoples R China.
关键词：
ABCC2 promoter;Bacillus thuringiensis;GATAe factor;Helicoverpa armigera;Regulation of gene expression
摘要：
Helicoverpa armigera is a worldwide pest that has been efficiently controlled by transgenic plants expressing Bt Cry toxins. To exert toxicity, Cry toxins bind to different receptors located in larval midgut cells. Previously, we reported that GATA transcription factor GATAe activates the expression of multiple H. armigera Cry1Ac receptors in different insect cell lines. Here, the mechanism involved in GATAe regulation of HaABCC2 gene expression, a key receptor of Cry1Ac, was analyzed. HaGATAe gene silencing by RNAi in H. armigera larvae confirmed the activation role of HaGATAe on the expression of HaABCC2 in the midgut. The contribution of all potential GATAe-binding sites was analyzed by site-directed mutagenesis using Hi5 cells expressing a reporter gene under regulation of different modified HaABCC2 promoters. DNA pull-down assays revealed that GATAe bound to different predicted GATA-binding sites and mutations of the different GATAe-binding sites identified two binding sites responsible for the promoter activity. The binding site B9, which is located near the transcription initiator site, has a major contribution on HaABCC2 expression. Also, DNA pull-down assays revealed that all other members of GATA TF family in H. armigera, besides GATAe, HaGATAa, HaGATAb, HaGATAc and HaGATAd also bound to the HaABCC2 promoter and decreased the GATAe dependent promoter activity. Finally, the potential participation in the regulation of HaABCC2 promoter of several TFs other than GATA TFs expressed in the midgut cells was analyzed. HaHR3 inhibited the GATAe dependent activity of the HaABCC2 promoter, while two other midgut-related TFs, HaCDX and HaSox21, also bound to the HaABCC2 promoter region and increased the GATAe dependent promoter activity. All these data showed that GATAe induces HaABCC2 expression by binding to HaGATAe binding sites in the promoter region and that additional TFs participate in modulating the HaGATAe-driven expression of HaABCC2.
摘要：
The field of genomics has ushered in new methods for studying molecular-genetic variation in natural populations. However, most population-genomic studies still rely on small sample sizes (typically, <100 individuals) from single time points, leaving considerable uncertainties with respect to the behavior of relatively young (and rare) alleles and, owing to the large sampling variance of measures of variation, to the specific gene targets of unusually strong selection. Genomic sequences of ∼1,700 haplotypes distributed over a 10-year period from a natural population of the microcrustacean Daphnia pulex reveal evolutionary-genomic features at a refined scale, including previously hidden information on the behavior of rare alleles predicted by recent theory. Background selection, resulting from the recurrent introduction of deleterious alleles, appears to strongly influence the dynamics of neutral alleles, inducing indirect negative selection on rare variants and positive selection on common variants. Temporally fluctuating selection increases the persistence of nonsynonymous alleles with intermediate frequencies, while reducing standing levels of variation at linked silent sites. Combined with the results from an equally large metapopulation survey of the study species, classes of genes that are under strong positive selection can now be confidently identified in this key model organism. Most notable among rapidly evolving Daphnia genes are those associated with ribosomes, mitochondrial functions, sensory systems, and lifespan determination.
摘要：
BACKGROUND: The insect hemolymph (blood-equivalent fluid), composed of a large number of hemocytes (blood cells) and a variety of soluble immune effectors, is hostile for pathogens including fungi. In order to survive in the insect hemocoel (body cavity), the entomopathogenic fungus (EPF) has evolved two classical coping strategies, namely evasion and suppression of the host immune reactions. However, it remains unclear whether EPF has other ways of coping with host immunity. RESULTS: In this study, we demonstrated that Metarhizium rileyi (an EPF) infection by injection of blastospores into the hemocoel enhanced the plasma antibacterial activity of cotton bollworm (Helicoverpa armigera), which was partially due to the enhanced expression of antimicrobial peptides (AMPs). The early stage of M. rileyi infection induced the translocation of gut bacteria into the hemocoel, where they were subsequently cleared due to the enhanced plasma antibacterial activity. Further, we showed that the enhanced plasma antibacterial activity and AMP expression were attributable to M. rileyi but not the invasive gut bacteria (opportunistic bacteria). Elevated ecdysone (major steroid hormone in insects) levels in the hemolymph at 48h post-M. rileyi infection might contribute to the enhanced expression of AMPs. The fungus-elicited AMPs, such as cecropin 3 or lebocin, exhibited potent inhibitory activity against the opportunistic bacteria but not against hyphal bodies. In addition, the opportunistic bacteria competed with hyphal bodies for amino acid nutrients. CONCLUSIONS: M. rileyi infection induced the translocation of gut bacteria, and then the fungi activated and exploited its host humoral antibacterial immunity to eliminate opportunistic bacteria, preventing them from competing for nutrients in the hemolymph. Unlike the classical strategies, EPF utilizes to evade or suppress host immunity, our findings reveal a novel strategy of interaction between EPF and host immunity. Video Abstract.
作者机构：
[Liao, Cai-Yi; Liu, Wen-Cheng; Song, Ru-Feng; Chen, Hui-Hui; Zhang, Yu; Guo, Jia-Xing; Lu, Kai-Kai; Zuo, Jia-Xin; Hu, Xiao-Yu] Henan Univ, Coll Life Sci, Collaborat Innovat Ctr Crop Stress Biol, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475004, Peoples R China.;[Ren, Feng] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Lu, Ying-Tang] Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan, Peoples R China.
通讯机构：
[Wen-Cheng Liu] S;State Key Laboratory of Crop Stress Adaptation and Improvement, Collaborative Innovation Center of Crop Stress Biology, College of Life Sciences, Henan University , Kaifeng 475004 , China
摘要：
SALT OVERLY SENSITIVE1 (SOS1) is a key component of plant salt tolerance. However, how SOS1 transcription is dynamically regulated in plant response to different salinity conditions remains elusive. Here, we report that C-type Cyclin1;1 (CycC1;1) negatively regulates salt tolerance by interfering with WRKY75-mediated transcriptional activation of SOS1 in Arabidopsis (Arabidopsis thaliana). Disruption of CycC1;1 promotes SOS1 expression and salt tolerance in Arabidopsis because CycC1;1 interferes with RNA polymerase II recruitment by occupying the SOS1 promoter. Enhanced salt tolerance of the cycc1;1 mutant was completely compromised by an SOS1 mutation. Moreover, CycC1;1 physically interacts with the transcription factor WRKY75, which can bind to the SOS1 promoter and activate SOS1 expression. In contrast to the cycc1;1 mutant, the wrky75 mutant has attenuated SOS1 expression and salt tolerance, whereas overexpression of SOS1 rescues the salt sensitivity of wrky75. Intriguingly, CycC1;1 inhibits WRKY75-mediated transcriptional activation of SOS1 via their interaction. Thus, increased SOS1 expression and salt tolerance in cycc1;1 were abolished by WRKY75 mutation. Our findings demonstrate that CycC1;1 forms a complex with WRKY75 to inactivate SOS1 transcription under low salinity conditions. By contrast, under high salinity conditions, SOS1 transcription and plant salt tolerance are activated at least partially by increased WRKY75 expression but decreased CycC1;1 expression. CycC1;1 forms a transcriptional repression complex with the transcription factor WRKY75 to downregulate SOS1 expression, thereby negatively regulating salt stress tolerance in Arabidopsis.
作者机构：
[Yang, Yi-Wen; Chen, Si-Zhuo; Huang, Da; Qiu, Bao-Sheng; Yu, Chen; Liu, Ke; Qiu, BS] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Yang, Yi-Wen; Chen, Si-Zhuo; Huang, Da; Qiu, Bao-Sheng; Yu, Chen; Liu, Ke; Qiu, BS] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Yang, Yi-Wen] Jiujiang Univ, Coll Pharm & Life Sci, Jiujiang 332000, Jiangxi, Peoples R China.;[Chen, Min] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.
通讯机构：
[Chen, M ] U;[Qiu, BS ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.
摘要：
Expanded orange carotenoid protein paralogs in subaerial Nostoc species functionally specialize as singlet oxygen quenchers or phycobilisome fluorescence quenchers to survive desiccation tolerance. Orange carotenoid protein (OCP) is a photoactive protein that participates in the photoprotection of cyanobacteria. There are 2 full-length OCP proteins, 4 N-terminal paralogs (helical carotenoid protein [HCP]), and 1 C-terminal domain-like carotenoid protein (CCP) found in Nostoc flagelliforme, a desert cyanobacterium. All HCPs (HCP1 to 3 and HCP6) from N. flagelliforme demonstrated their excellent singlet oxygen quenching activities, in which HCP2 was the strongest singlet oxygen quencher compared with others. Two OCPs, OCPx1 and OCPx2, were not involved in singlet oxygen scavenging; instead, they functioned as phycobilisome fluorescence quenchers. The fast-acting OCPx1 showed more effective photoactivation and stronger phycobilisome fluorescence quenching compared with OCPx2, which behaved differently from all reported OCP paralogs. The resolved crystal structure and mutant analysis revealed that Trp111 and Met125 play essential roles in OCPx2, which is dominant and long acting. The resolved crystal structure of OCPx2 is maintained in a monomer state and showed more flexible regulation in energy quenching activities compared with the packed oligomer of OCPx1. The recombinant apo-CCP obtained the carotenoid pigment from holo-HCPs and holo-OCPx1 of N. flagelliforme. No such carotenoid transferring processes were observed between apo-CCP and holo-OCPx2. The close phylogenetic relationship of OCP paralogs from subaerial Nostoc species indicates an adaptive evolution toward development of photoprotection: protecting cellular metabolism against singlet oxygen damage using HCPs and against excess energy captured by active phycobilisomes using 2 different working modes of OCPx.
通讯机构：
[Haobin Zhao] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China
摘要：
Mep50 as a partner promotes the activity and substrate affinity of Prmt5. Prmt5 and Mep50 function together in multiple bioprocesses of the cells. Both Prmt5 and Mep50 are necessary for maintenance of the stem cells and are indispensable in the embryogenesis in the mammals. However, the role of Mep50 is rarely studied in fish. This study was to investigate the role of Mep50 in embryonic development of medaka. Medaka mep50 was mutated by genomic editing with CRISPR-Cas9 technology. Two mutants with a deletion of 22 and 46 bp separately in mep50 caused premature stopping of translation. The homozygotes of these mutant fish were obtained by self-crossing of the heterozygotes. These homozygotic mutants could reproduce embryos but the offspring were not viable. The apoptotic cells were significantly more in the mutant embryos than that in the wild type indicated by TUNEL assay. Quantitative RT-PCR showed that the expression of oct4 and sox2 were significantly decreased, but p53 was increased in the mutant embryos. These results suggest that disruption of mep50 severely interferes with embryogenesis and mep50 is necessary for embryonic development by maintaining stem cells and repression of apoptosis in medaka.
作者机构：
[Xiao, Yutao; Liu, Conghui; Liu, Zhenxing; Fan, Wei; Jin, Minghui; Wang, Ping; Liu, Bo; Wu, Chao; Wu, Shigang; Liu, Hangwei; He, Yuan; Chakrabarty, Swapan; Zheng, Weigang] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Key Lab Gene Editing Technol Hainan, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr,M, Shenzhen, Peoples R China.;[Jin, Minghui; Wu, Kongming; He, Yuan; Li, Xiaokang] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.;[Yuan, Haibin; Zheng, Weigang] Jilin Agr Univ, Coll Agron, Changchun 130118, Peoples R China.;[Liu, Conghui] Univ Hong Kong, Dept Clin Oncol, Hong Kong 999077, Peoples R China.;[Liu, Kaiyu] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Kongming Wu] T;[Wei Fan; Yutao Xiao] S;The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China<&wdkj&>Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China<&wdkj&>Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
摘要：
BACKGROUND: The black cutworm, Agrotis ipsilon, is a serious global underground pest. Its distinct phenotypic traits, especially its polyphagy and ability to migrate long distances, contribute to its widening distribution and increasing difficulty of control. However, knowledge about these traits is still limited. RESULTS: We generated a high-quality chromosome-level assembly of A. ipsilon using PacBio and Hi-C technology with a contig N50 length of ~ 6.7Mb. Comparative genomic and transcriptomic analyses showed that detoxification-associated gene families were highly expanded and induced after insects fed on specific host plants. Knockout of genes that encoded two induced ABC transporters using CRISPR/Cas9 significantly reduced larval growth rate, consistent with their contribution to host adaptation. A comparative transcriptomic analysis between tethered-flight moths and migrating moths showed expression changes in the circadian rhythm gene AiCry2 involved in sensing photoperiod variations and may receipt magnetic fields accompanied by MagR and in genes that regulate the juvenile hormone pathway and energy metabolism, all involved in migration processes. CONCLUSIONS: This study provides valuable genomic resources for elucidating the mechanisms involved in moth migration and developing innovative control strategies.
关键词：
active season;environmental bacterial reservoir;hibernation;neutral model;Pseudogymnoascus destructans;skin microbiota
摘要：
Skin microbiota play an important role in protecting bat hosts from the fungal pathogen Pseudogymnoascus destructans, which has caused dramatic bat population declines and extinctions. Recent studies have provided insights into the bacterial communities of bat skin, but variation in skin bacterial community structure in the context of the seasonal dynamics of fungal invasion, as well as the processes that drive such variation, remain largely unexplored. In this study, we characterized bat skin microbiota over the course of the bat hibernation and active season stages and used a neutral model of community ecology to determine the relative roles of neutral and selective processes in driving microbial community variation. Our results showed significant seasonal shifts in skin community structure, as well as less diverse microbiota in hibernation than in the active season. Skin microbiota were influenced by the environmental bacterial reservoir. During both the hibernation and active season stages, more than 78% of ASVs in bat skin microbiota were consistent with neutral distribution, implying that neutral processes, that is, dispersal or ecological drift contributing the most to shifts in skin microbiota. In addition, the neutral model showed that some ASVs were actively selected by the bats from the environmental bacterial reservoir, accounting for approximately 20% and 31% of the total community during hibernation and active season stages, respectively. Overall, this research provides insights into the assemblage of bat-associated bacterial communities and will aid in the development of conservation strategies against fungal disease.
作者机构：
[Wang, Xin-Wei; Jiang, Hai-Bo; Liu, Ling-Mei; Yong, Cheng-Wen] Ningbo Univ, Sch Marine Sci, Key Lab Marine Biotechnol Zhejiang Prov, Ningbo, Zhejiang, Peoples R China.;[Wang, Xin-Wei; Jiang, Hai-Bo; Yong, Cheng-Wen] Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai, Guangdong, Peoples R China.;[Jiang, Hai-Bo; Liu, Ling-Mei; Deng, Bin] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.;[Jiang, Hai-Bo] Minist Nat Resources, Inst Oceanog 2, State Key Lab Satellite Ocean Environm Dynam, Hangzhou, Zhejiang, Peoples R China.
通讯机构：
[Xin-Wei Wang; Hai-Bo Jiang] K;Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Science, Ningbo University, Ningbo, Zhejiang, China<&wdkj&>Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, Guangdong, China<&wdkj&>Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Science, Ningbo University, Ningbo, Zhejiang, China<&wdkj&>Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, Guangdong, China<&wdkj&>School of Life Sciences, Central China Normal University, Wuhan, Hubei, China<&wdkj&>State Key Laboratory of Satellite Ocean Environment Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, Zhejiang, China
作者机构：
[Luo, Jingwen; Li, Mengyun; Chen, Feng; Xu, Wenliang; Gao, Yanan; Guo, Yanjun; Qiao, Mengfei; Xu, WL] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.;[Guo, Yanjun] Xinjiang Normal Univ, Coll Life Sci, Urumqi, Peoples R China.;[Persson, Staffan] Univ Copenhagen, Copenhagen Plant Sci Ctr, Frederiksberg, Denmark.;[Persson, Staffan] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Joint Int Res Lab Metab & Dev Sci, Shanghai, Peoples R China.;[Zeng, Wei; Zeng, W] Taizhou Univ, Sch Life Sci, Taizhou, Peoples R China.
通讯机构：
[Zeng, W ] T;[Xu, WL ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.;Taizhou Univ, Sch Life Sci, Taizhou, Peoples R China.
通讯作者：
Cuihong Wan<&wdkj&>Cuihong Wan Cuihong Wan Cuihong Wan
作者机构：
[Wan, Cuihong; Huwanixi, Aishuake; Peng, Zhao] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.;[Wan, Cuihong; Huwanixi, Aishuake; Peng, Zhao] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.;[Wan, Cuihong] Cent China Normal Univ, Sch Life Sci, 152 Luoyu Rd, Wuhan 430079, Peoples R China.;[Wan, Cuihong] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Cuihong Wan; Cuihong Wan Cuihong Wan Cuihong Wan] S;School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, Hubei, PR China
摘要：
Nostoc flagelliforme, a terrestrial cyanobacterium spread throughout arid and semi-arid areas, has been long known for its outstanding adaptability to extremely dry conditions. This microorganism is able to recover biological activities within hours after months of anhydrobiosis state, attracting investigation through proteomic analysis. Except for canonical proteome, microproteins encoded by small ORFs (smORFs) have recently been regarded as indispensable participants in metabolic processes. However, the involvement of smORFs in N. flagelliforme remains unknown. Here we first constructed a smORF database in N. flagelliforme using bioinformatic prediction, resulting in 6072 novel smORFs. Then LS-MS/MS analysis was applied to identify expression patterns of microproteins and seek smORFs and their encoded microprotein playing a role during rehydration. In total, 18 novel microproteins were mined based on a smORF searching strategy combined with three proteomic assays, of which five were annotated as ribosomal proteins, one as RNA polymerase subunit, and one as acetohydroxy acid isomeroreductase. We also suggested the possible functions of smORFs according to their expression pattern and discovered two neighboring and homologous smORFs. All these results will expand our knowledge of smORFs-encoded microproteins and their relation to the stress response of extremophilic microorganisms.
作者机构：
[Li, Yiming; Niu, Meiling; Yang, Jiaxue; Zhang, Jinyu; Li, Yuanyi; Li, YM; Du, Jiacong; Zhang, Jun] Hebei Univ, Inst Life Sci & Green Dev, Sch Life Sci, Baoding 071002, Peoples R China.;[Li, Yiming; Song, Tianjian; Wang, Siqi; Luo, Zexu; Li, Wenhao; Li, YM; Deng, Teng] Chinese Acad Sci, Inst Zool, Key Lab Anim Ecol & Conservat Biol, 1 Beichen West Rd, Beijing 100101, Peoples R China.;[Li, Yiming; Song, Tianjian; Wang, Siqi; Luo, Zexu; Li, Wenhao; Li, YM; Deng, Teng] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Blackburn, Tim M.] UCL, Ctr Biodivers & Environm Res, Gower St, London WC1E 6BT, England.;[Blackburn, Tim M.] Zool Soc London, Inst Zool, Regents Pk, London NW1 4RY, England.
通讯机构：
[Li, YM ] H;Hebei Univ, Inst Life Sci & Green Dev, Sch Life Sci, Baoding 071002, Peoples R China.;Chinese Acad Sci, Inst Zool, Key Lab Anim Ecol & Conservat Biol, 1 Beichen West Rd, Beijing 100101, Peoples R China.;Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
摘要：
The global trade in live wildlife elevates the risk of biological invasions by increasing colonization pressure (the number of alien species introduced to an area). Yet, our understanding of species traded as aliens remains limited. We created a comprehensive global database on live terrestrial vertebrate trade and use it to investigate the number of traded alien species, and correlates of establishment richness for aliens. We identify 7,780 species involved in this trade globally. Approximately 85.7% of these species are traded as aliens, and 12.2% of aliens establish populations. Countries with greater trading power, higher incomes, and larger human populations import more alien species. These countries, along with island nations, emerge as hotspots for establishment richness of aliens. Colonization pressure and insularity consistently promote establishment richness across countries, while socio-economic factors impact specific taxa. Governments must prioritize policies to mitigate the release or escape of traded animals and protect global biosecurity. This study compiled a comprehensive global database on live terrestrial vertebrate trade and used it to investigate traded alien species. The authors identify 7,780 species involved in trade globally and show that countries with greater trading power, higher incomes and larger human populations import more alien species, which emerge as hotspots for establishment richness of aliens.
作者：
Cao, Ya Ya;Chen, Yuan Yuan;Wang, Ming Shu;Tong, Jing Jing;Xu, Meng;...
期刊：
Redox Biology,2023年63:102751 ISSN：2213-2317
通讯作者：
Zhang, Dan;Yang, GF
作者机构：
[Chen, Yuan Yuan; Zhang, Wen Lin; Yang, Guang Fu; Lin, Hong Yan; Mei, Long Can; Wang, Ming Shu; Dong, Jin; Zhang, Dan; Zhang, D; Cao, Ya Ya; Huang, Wei; Qin, Yu Xuan; Zhao, Chi] Cent China Normal Univ, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.;[Tong, Jing Jing; Xu, Meng] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.;[Chen, Yuan Yuan] Hunan Univ, Inst Chem Biol & Nanomed, Coll Chem & Chem Engn, Changsha 410082, Peoples R China.
通讯机构：
[Zhang, D; Yang, GF ] C;Cent China Normal Univ, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.
关键词：
Catalase inhibitors;NADPH-Binding site;Ferroptosis;Castration-resistant prostate cancer
摘要：
Catalase (CAT) is an important antioxidant enzyme that breaks down H(2)O(2) into water and oxygen. Inhibitor-modulating CAT activity in cancer cells is emerging as a potential anticancer strategy. However, the discovery of CAT inhibitors towards the heme active center located at the bottom of long and narrow channel has made little progress. Therefore, targeting new binding site is of great importance for the development of efficient CAT inhibitors. Here, the first NADPH-binding site inhibitor of CAT, BT-Br, was designed and synthesized successfully. The cocrystal structure of BT-Br-bound CAT complex was determined with a resolution of 2.2Å (PDB ID:8HID), which showed clearly that BT-Br bound at the NADPH-binding site. Furthermore, BT-Br was demonstrated to induce ferroptosis in castration-resistant prostate cancer (CRPC) DU145cells and eventually reduce CRPC tumors in vivo effectively. The work indicates that CAT has potential as a novel target for CRPC therapy based on ferroptosis inducing.
作者机构：
[Fu, Ziying; Fan, Zihui; Cui, Zhongdan; Chen, Qicai; Zhang, Guimin] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Yang, Lijian; Jia, Ya] Cent China Normal Univ, Coll Phys Sci & Technol, Wuhan 430079, Peoples R China.
通讯机构：
[Ziying Fu] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, Hubei 430079, China
摘要：
The Lombard effect, ref erring to an involuntary rise in vocal intensity, is a widespread vertebrate mecha-nism that aims to maintain signal efficiency in response to ambient noise. Previous studies showed that the Lombard effect could be sufficiently implemented at subcortical levels and operated by continuously monitoring background noise, requiring some subcortical auditory sensitive neurons to have continuous responses to background noise. However, such neurons have not been well characterized. The inferior colliculus (IC) is a major auditory integration center under the auditory cortex and provides projections to the putative vocal pattern generator in the brainstem. Thus, it is reasonable to speculate that the IC is a likely auditory nucleus candidate having background noise responding neurons (BNR neurons). In the present study, we isolated 183 sound-sensitive IC neurons in a constant frequency-frequency mod-ulation bat, Hipposideros pratti, and found that around 19% of these IC neurons are BNR neurons when stimulated with 70 dB SPL background white noise. Their firing rates in response to noise increased with increasing noise intensity and could be suppressed by sound stimulation. Furthermore, compared to neu-rons with similar best frequencies, the BNR neurons had smaller Q 10-dB values and lower noise-induced minimal threshold change, indicating that BNR neurons received fewer inhibitory inputs. These results suggested that the BNR neurons are ideal candidates for collecting information about background noise. We proposed that the BNR neurons synapsed with neurons in vocal-pattern-generating networks in the brainstem and initiated the Lombard effect by a feed-forward loop. (c) 2023 Elsevier B.V. All rights reserved.
