摘要：
Brassinosteroids (BRs) play critical roles in plant growth and development and regulate many important agronomic traits. However, the functions of BRs in strawberry are unclear. This study identified two mutants, named P6 and R87, in woodland strawberry (Fragaria vesca) from EMS mutagenesis populations that exhibit narrow leaves, petals and sepals. Mapping by sequencing and genetic studies revealed that the F. vesca CYP734A129, encoding a putative BR catabolic enzyme, is the causative gene for both P6 and R87. Overexpression of CYP734A129 in both F. vesca and Arabidopsis causes a severe dwarf phenotype, and the BRI1-EMS-SUPPRESSOR 1 (BES1) protein is less abundant in the CYP734A129-overexpressing Arabidopsis seedlings. This suggests that CYP734A129 is functionally conserved with CYP734A1, as a BR-inactivating enzyme. Transcriptome analysis of young leaves revealed that four BR biosynthetic genes were significantly downregulated in P6 (cyp734a129), and photosynthesis-related genes were highly enriched among the up-regulated genes in P6 compared to the wild type. This further supports that CYP734A129 inactivates BRs in F. vesca. Furthermore, we showed that mutations in CYP734A129 do not affect fruit shape and color during ripening in strawberry. Overall, our results suggest that F. vesca CYP734A129 is a BR catabolic enzyme, and provide insights into the roles of CYP734A129 in strawberry.
作者机构：
[Yang, Decai; Zhang, Bin; Zhang, Yuanyuan; Li, Chenxi; Nie, Guohui; Yang, DC; Fang, Xueyang] Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Shenzhen Inst Translat Med,Guangdong Key Lab Biome, Shenzhen 518035, Peoples R China.;[Lo, Wai-Sum; Wong, Wing-Tak; Lui, Kwok-Ho; Gu, Yanjuan; Li, Xin; Wong, WT; Fang, Xueyang; Gu, YJ] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Hong Kong, Peoples R China.;[Wu, Xianlin] Guangzhou Univ Chinese Med, Shenzhen Hosp, Canc Ctr, Shenzhen 518000, Peoples R China.;[Wu, Xianlin] Jinan Univ, Affiliated Hosp 1, Inst Clin Med, Guangzhou 510632, Peoples R China.;[Jiang, Lijun] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Zhang, B ; Yang, DC] S;[Jiang, LJ ] C;[Gu, YJ; Wong, WT ] H;Shenzhen Univ, Shenzhen Peoples Hosp 2, Affiliated Hosp 1, Shenzhen Inst Translat Med,Guangdong Key Lab Biome, Shenzhen 518035, Peoples R China.;Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Hong Kong, Peoples R China.
关键词：
Dual single-atom catalysts;Tumor microenvironment;H2O2 activation;Hypoxia alleviation;Synergistic cancer therapy
摘要：
Tumor therapy employing metal-based nanomaterials to convert the abundant H2O2 in tumor microenvironment (TME) to oxygen (O2) and hydroxy radical (& BULL;OH) has attracted substantial attention. However, the generally complex structure of metal nanosystems may have poor catalytic selectivity towards the target and hence cause undesired side reactions. Single-atom catalysts (SACs) with high atomic utilization, composition of identical active site and tunable reaction pathway can be harnessed to realize the well-controlled and highly-selective conversion of H2O2 for cancer therapy. Herein, a series of dual single-atom catalysts (DSACs) containing two metal centers (Fe-Se6, Mn-Se6) are proposed. As guided by theoretical calculations, DSACs with equal proportions of Fe and Mn (termed as Fe/Mn@PSe3) exhibit the optimal reaction barriers towards the production of both & BULL;OH and O2 by catalyzing H2O2. In response to the elevated TME H2O2, Fe/Mn@PSe3 can produce & BULL;OH to trigger chemodynamic therapy (CDT), and boost O2 generation to alleviate tumor hypoxia and its mediated immunosuppression. In addition, its mild hyperthermia feature enhances the anti-tumor effects of CDT and immune therapy, causing an efficient synergistic tumor suppression outcome. The study provides new insights into highly selective nanomaterial design that preferentially activating specific catalytic processes within tumor, functioning as promising candidates for cancer therapy.
期刊：
Pesticide Biochemistry and Physiology,2023年194:105516 ISSN：0048-3575
通讯作者：
Yang, Yongbo;Liu, KY
作者机构：
[Xia, Zhichao; Liu, Kaiyu; Wei, Wei; Wang, Haixia; Yang, Yongbo; Pan, Shuang; Liu, Leilei] Cent China Normal Univ, Sch Life Sci, Wuhan 430070, Peoples R China.;[Wei, Wei; Liu, Leilei] Wuhan Univ Bioengn, Appl Biotechnol Ctr, Wuhan 430415, Peoples R China.;[Wang, Ling] Inst Hubei Agr Acad, Wuhan 430070, Peoples R China.;[Xiao, Yutao] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China.;[Bravo, Alejandra] Univ Nacl Autonoma Mexico, Inst Biotecnol, Apdo Postal 510-3, Cuernavaca 62250, Morelos, Mexico.
通讯机构：
[Yang, YB; Liu, KY ] C;Cent China Normal Univ, Inst Entomol, Sch Life Sci, Wuhan 430070, Peoples R China.
关键词：
ABCC2 promoter;Bacillus thuringiensis;GATAe factor;Helicoverpa armigera;Regulation of gene expression
摘要：
Helicoverpa armigera is a worldwide pest that has been efficiently controlled by transgenic plants expressing Bt Cry toxins. To exert toxicity, Cry toxins bind to different receptors located in larval midgut cells. Previously, we reported that GATA transcription factor GATAe activates the expression of multiple H. armigera Cry1Ac receptors in different insect cell lines. Here, the mechanism involved in GATAe regulation of HaABCC2 gene expression, a key receptor of Cry1Ac, was analyzed. HaGATAe gene silencing by RNAi in H. armigera larvae confirmed the activation role of HaGATAe on the expression of HaABCC2 in the midgut. The contribution of all potential GATAe-binding sites was analyzed by site-directed mutagenesis using Hi5 cells expressing a reporter gene under regulation of different modified HaABCC2 promoters. DNA pull-down assays revealed that GATAe bound to different predicted GATA-binding sites and mutations of the different GATAe-binding sites identified two binding sites responsible for the promoter activity. The binding site B9, which is located near the transcription initiator site, has a major contribution on HaABCC2 expression. Also, DNA pull-down assays revealed that all other members of GATA TF family in H. armigera, besides GATAe, HaGATAa, HaGATAb, HaGATAc and HaGATAd also bound to the HaABCC2 promoter and decreased the GATAe dependent promoter activity. Finally, the potential participation in the regulation of HaABCC2 promoter of several TFs other than GATA TFs expressed in the midgut cells was analyzed. HaHR3 inhibited the GATAe dependent activity of the HaABCC2 promoter, while two other midgut-related TFs, HaCDX and HaSox21, also bound to the HaABCC2 promoter region and increased the GATAe dependent promoter activity. All these data showed that GATAe induces HaABCC2 expression by binding to HaGATAe binding sites in the promoter region and that additional TFs participate in modulating the HaGATAe-driven expression of HaABCC2.
期刊：
Food and Chemical Toxicology,2023年173:113642 ISSN：0278-6915
通讯作者：
Mingqing Chen
作者机构：
[Xu, Ke; Chen, Mingqing; Han, Qi; Wang, Xuan; Gao, Xiao; Wei, Zhaolan; Wang, Yunyi; Wang, Shuwei] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Mingqing Chen] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, Hubei, China
作者机构：
[Mao, Bin; Chen, Meng-Yan; Wang, Qian; Shen, Wei; Li, Chao; Wang, Yu-Feng; Zheng, Ya; Duan, Xin] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, YF ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Ocnus;Drosophila melanogaster;Proteomics;Phosphoproteomics;Testis development
摘要：
Testis is the only organ supporting sperm production and with the largest number of proteins and tissue-specific proteins in animals. In our previous studies, we have found that knockdown of ocnus (ocn), a testis-specific gene, resulted in much smaller testis with no germ cells in Drosophila melanogaster. However, the molecular consequences of ocn knockdown in fly testes are unknown. In this study, through iTRAQ quantitative proteomics sequencing, 606 proteins were identified from fly abdomens as having a significant and at least a 1.5-fold change in expression after ocn knockdown in fly testes, of which 85 were up-regulated and 521 were down-regulated. Among the differential expressed proteins (DEPs), apart from those proteins involved in spermatogenesis, the others extensively affected biological processes of generation of precursor metabolites and energy, metabolic process, and mitochondrial transport. Protein-protein interaction (PPI) analyses of DEPs showed that several kinases and/or phosphatases interacted with Ocn. Re-analyses of the transcriptome revealed 150 differential expressed genes (DEGs) appeared in the DEPs, and their changing trends in expressions after ocn knockdown were consistent. Many common down-regulated DEGs and DEPs were testis-specific or highly expressed in the testis of D. melanogaster. Quantitative RT-PCR (qRT-PCR) confirmed 12 genes appeared in both DEGs and DEPs were significantly down-regulated after ocn knockdown in fly testes. Furthermore, 153 differentially expressed phosphoproteins (DEPPs), including 72 up-regulated and 94 down-regulated phosphorylated proteins were also identified (13 phosphoproteins appeared in both up- and down-regulated groups due to having multiple phosphorylation sites). In addition to those DEPPs associated with spermatogenesis, the other DEPPs were enriched in actin filament-based process, protein folding, and mesoderm development. Some DEPs and DEPPs were involved in Notch, JAK/STAT, and cell death pathways. Given the drastic effect of the ocn knockdown on tissue development and testis cells composition, the differences in protein abundance in the ocn knockdown flies might not necessarily be the direct result of differential gene regulation due to the inactivation of ocn. Nevertheless, our results suggest that the expression of ocn is essential for Drosophila testis development and that its down-regulation disturbs key signaling pathways related to cell survival and differentiation. These DEPs and DEPPs identified may provide significant candidate set for future studies on the mechanism of male reproduction of animals, including humans.
摘要：
Understanding trophic niche differentiation is critical for exploring interspecific competition and stable coexistence among morphologically similar sympatric species. Bats are an ideal model for studying trophic niche differentiation among species because of their high taxonomic and ecological diversities, as well as their special life history traits. Although many factors can affect bat trophic niches, few studies have combined multiple factors to investigate the influences on bat trophic niches. In this study, we analyzed the summer diet and potential influencing factors of five sympatric rhinolophid bats in southwestern China: Rhinolophus macrotis, Rhinolophus osgoodi, Rhinolophus ferrumequinum, Rhinolophus affinis, and Rhinolophus pusillus. All five species mainly fed on Lepidopteran and Dipteran insects, with a large trophic niche breadth for each species and a low degree of dietary overlap. With the exception of R. affinis and R. pusillus, significant differences in diet composition were detected among species, which indicated relatively low interspecific competition. Canonical correspondence analysis showed that both echolocation calls and body size significantly affected interspecific diet differentiation, while wing morphology and bite force had relatively weak effects. This study suggests that the combined effects of multiple factors may drive trophic niche differentiation among five rhinolophid bat species in the study area.
期刊：
American Journal of Botany,2023年110(6) ISSN：0002-9122
通讯作者：
Huang, SQ
作者机构：
[Huang, Shuang-Quan; Huang, SQ; Hao, Kai] Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.;[Hao, Kai] Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable Ut, South China Bot Garden, Guangzhou 510650, Peoples R China.;[Fang, Qiang] Henan Univ Sci & Technol, Coll Agr, Luoyang 471003, Peoples R China.
通讯机构：
[Huang, SQ ] C;Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.
关键词：
Caryophyllaceae;conspecific pollen limitation;generalized flower;heterospecific pollen deposition;pollen fitness;pollen species diversity;seed set;Silene;tolerance and avoidance strategy
摘要：
Abstract Premise Co‐flowering species that have not evolved an avoidance mechanism may have tolerance to heterospecific pollen (HP) deposition as an adaptive strategy to minimize any deleterious effects of HP transfer, but empirical evidence for the tolerance hypothesis remains scarce. Methods To estimate the potential effects of heterospecific pollen deposition (HPD) on female reproductive success, we counted conspecific (CP) and HP pollen grains deposited on stigmas and assessed subsequent seed set of both open‐ and hand‐pollinated flowers in three co‐flowering Silene species with exposed stigmas that usually received numerous HP grains on the elongated receptive area. Results The percentage of HP grains per flower (HP%) varied from 16.6% to 43.0% among three species. Silene chungtienensis had lower HP%, and the CP‐HP relationship was neutral; S. gracilicaulis and S. yunnanensis had a relatively higher HP% with a positive CP‐HP relationship. The effects of CP and HP number on natural seed set were positive for all three species, but HP% had stronger negative effects in S. chungtienensis and S. gracilicaulis. In hand‐pollinated flowers of the three Silene species, seed set did not decrease with HP whether CP was in excess or insufficient, indicating no negative effects of HPD on seed production. Conclusions Consistent with the tolerance hypothesis, our results indicated that species with higher HP interference are likely to be tolerant to an increase in HP%. These species with generalist‐pollinated flowers and exposed large stigmas may benefit from an increase of conspecific pollen deposition, despite the associated increase in heterospecific pollen deposition.
摘要：
BACKGROUND AND AIMS: Morphological matching between flower and pollinator traits has been documented in diverse plant lineages. Indeed, the matching of corolla-tube length and pollinator-tongue length has been cited repeatedly as a classic case of coevolution. However, there are many possible evolutionary routes to trait matching. Our aim here is both to review the evolutionary mechanisms of plant-pollinator trait matching and to investigate a specific case of trait matching/mismatching in a genus of alpine gingers. METHODS: Roscoea gingers with long corolla tubes in the western Himalayas have pollinators with correspondingly long tongues, but the match between corolla-tube and pollinator-tongue lengths is not seen in the eastern Himalayas. Six floral traits were measured, including corolla-tube depth, an internal trait controlling pollinator access to nectar. We calculated coefficients of variation (CVs) and phylogenetically controlled correlation patterns of these traits in six Roscoea species in order to gain possible insights into stabilizing selection and modularization of these traits. KEY RESULTS: The distal (nectar-containing) portion of the corolla tube exhibited lower CVs that did the basal portion. This is consistent with the hypothesis that pollinators mediate stabilizing selection on the distal, but not basal, portion of the corolla tube. This result, combined with phylogenetic data, suggests that the elevated liquid level of nectar in the distal tube evolved subsequent to dispersal into the eastern Himalayan region and loss of long-tongue pollinators. After accounting for phylogeny, corolla-tube length, anther length, style length, and labellum width were all intercorrelated. Corolla-tube depth was not part of this covariational module, however, suggesting separate adaptation to short-tongued pollinators. CONCLUSIONS: The reduction in functional corolla-tube depth in the Roscoea appears to be related to the loss of long-tongue pollinators associated with dispersal to the eastern Himalayas and pollination by short-tongued pollinators. The apparent mismatch between floral tubes and pollinator tongues is a case of cryptic trait matching between flowers and pollinators, underscoring the importance of combining floral anatomy with pollination ecology, in assessing plant-pollinator trait matching.
通讯机构：
[Xie, GS ] H;[Zhang, ZF ] C;Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China.;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
de novo fatty acid synthesis;fibrillin 7;galactolipids;KAS I;plastoglobule;rice
摘要：
Plastoglobules (PGs) contiguous with the outer leaflets of thylakoid membranes regulate lipid metabolism, plastid developmental transitions, and responses to environmental stimuli. However, the function of OsFBN7, a PG-core fibrillin gene in rice, has not been elucidated. Using molecular genetics and physiobiochemical approaches, we observed that OsFBN7 overexpression promoted PG clustering in rice chloroplasts. OsFBN7 interacted with two KAS I enzymes, namely OsKAS Ia and OsKAS Ib, in rice chloroplasts. Lipidomic analysis of chloroplast subcompartments, including PGs in the OsFBN7 overexpression lines, confirmed that levels of diacylglycerol (DAG), a chloroplast lipid precursor and monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), the main chloroplast membrane lipids, were increased in PGs and chloroplasts. Furthermore, OsFBN7 enhanced the abundances of OsKAS Ia/Ib in planta and their stability under oxidative and heat stresses. In addition, RNA sequencing and real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analyses showed that the expression of the DAG synthetase gene PAP1 and MGDG synthase gene MDG2 was upregulated by OsFBN7. In conclusion, this study proposes a new model in which OsFBN7 binds to OsKAS Ia/Ib in chloroplast and enhances their abundance and stability, thereby regulating the chloroplast and PG membrane lipids involved in the formation of PG clusters.
摘要：
BACKGROUND: The insect hemolymph (blood-equivalent fluid), composed of a large number of hemocytes (blood cells) and a variety of soluble immune effectors, is hostile for pathogens including fungi. In order to survive in the insect hemocoel (body cavity), the entomopathogenic fungus (EPF) has evolved two classical coping strategies, namely evasion and suppression of the host immune reactions. However, it remains unclear whether EPF has other ways of coping with host immunity. RESULTS: In this study, we demonstrated that Metarhizium rileyi (an EPF) infection by injection of blastospores into the hemocoel enhanced the plasma antibacterial activity of cotton bollworm (Helicoverpa armigera), which was partially due to the enhanced expression of antimicrobial peptides (AMPs). The early stage of M. rileyi infection induced the translocation of gut bacteria into the hemocoel, where they were subsequently cleared due to the enhanced plasma antibacterial activity. Further, we showed that the enhanced plasma antibacterial activity and AMP expression were attributable to M. rileyi but not the invasive gut bacteria (opportunistic bacteria). Elevated ecdysone (major steroid hormone in insects) levels in the hemolymph at 48h post-M. rileyi infection might contribute to the enhanced expression of AMPs. The fungus-elicited AMPs, such as cecropin 3 or lebocin, exhibited potent inhibitory activity against the opportunistic bacteria but not against hyphal bodies. In addition, the opportunistic bacteria competed with hyphal bodies for amino acid nutrients. CONCLUSIONS: M. rileyi infection induced the translocation of gut bacteria, and then the fungi activated and exploited its host humoral antibacterial immunity to eliminate opportunistic bacteria, preventing them from competing for nutrients in the hemolymph. Unlike the classical strategies, EPF utilizes to evade or suppress host immunity, our findings reveal a novel strategy of interaction between EPF and host immunity. Video Abstract.
作者机构：
[Huang, Rong; Deng, Youchao; Iyamu, Iredia D.] Purdue Univ, Inst Drug Discovery, Ctr Canc Res, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA.;[Dong, Aiping; Min, Jinrong; Song, Xiaosheng] Univ Toronto, Dept Physiol, Struct Genom Consortium, Toronto, ON M5G 1L7, Canada.;[Dong, Aiping; Min, Jinrong; Song, Xiaosheng] Univ Toronto, Dept Physiol, Toronto, ON M5G 1L7, Canada.;[Min, Jinrong] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Deng, Youchao] Cent South Univ, Xiangya Sch Pharmaceut Sci, Changsha 410013, Peoples R China.
通讯机构：
[Huang, R ] P;[Min, JR ] U;Purdue Univ, Inst Drug Discovery, Ctr Canc Res, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA.;Univ Toronto, Dept Physiol, Struct Genom Consortium, Toronto, ON M5G 1L7, Canada.;Univ Toronto, Dept Physiol, Toronto, ON M5G 1L7, Canada.
摘要：
Protein arginine methyltransferases (PRMTs) are attractive targets for developing therapeutic agents, but selective PRMT inhibitors targeting the cofactor SAM binding site are limited. Herein, we report the discovery of a noncanonical but less polar SAH surrogate YD1113 by replacing the benzyl guanidine of a pan-PRMT inhibitor with a benzyl urea, potently and selectively inhibiting PRMT3/4/5. Importantly, crystal structures reveal that the benzyl urea moiety of YD1113 induces a unique and novel hydrophobic binding pocket in PRMT3/4, providing a structural basis for the selectivity. In addition, YD1113 can be modified by introducing a substrate mimic to form a "T-shaped" bisubstrate analogue YD1290 to engage both the SAM and substrate binding pockets, exhibiting potent and selective inhibition to type I PRMTs (IC50 < 5 nmol/L). In summary, we demonstrated the promise of YD1113 as a general SAH mimic to build potent and selective PRMT inhibitors.(c) 2023 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
摘要：
Time delay and coupling strength are important factors that affect the synchronization of neural networks. In this study, a modular neural network containing subnetworks of different scales was constructed using the Hodgkin-Huxley (HH) neural model; i.e., a small-scale random network was unidirectionally connected to a large-scale small-world network through chemical synapses. Time delays were found to induce multiple synchronization transitions in the network. An increase in coupling strength also promoted synchronization of the network when the time delay was an integer multiple of the firing period of a single neuron. Considering that time delays at different locations in a modular network may have different effects, we explored the influence of time delays within each subnetwork and between two subnetworks on the synchronization of modular networks. We found that when the subnetworks were well synchronized internally, an increase in the time delay within both subnetworks induced multiple synchronization transitions of their own. In addition, the synchronization state of the small-scale network affected the synchronization of the large-scale network. It was surprising to find that an increase in the time delay between the two subnetworks caused the synchronization factor of the modular network to vary periodically, but it had essentially no effect on the synchronization within the receiving subnetwork. By analyzing the phase difference between the two subnetworks, we found that the mechanism of the periodic variation of the synchronization factor of the modular network was the periodic variation of the phase difference. Finally, the generality of the results was demonstrated by investigating modular networks at different scales.
期刊：
Physiological and Molecular Plant Pathology,2023年125:102002 ISSN：0885-5765
通讯作者：
Za Khai Tuang
作者机构：
[Tuang, Za Khai] Cent China Normal Univ, Sch Life Sci, Wuhan, Peoples R China.;[Tuang, Za Khai] Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, Dept Plant Pathol & Microbiol, Rehovot, Israel.;[Ling, Tial C.] Chiang Mai Univ, Fac Sci, Dept Biol, Bee Protect Lab, Chiang Mai 50200, Thailand.
通讯机构：
[Za Khai Tuang] S;School of Life Sciences, Central China Normal University, Wuhan, China<&wdkj&>Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
摘要：
The adaptation process is a way of life for plants, and they must contend with biotic and abiotic stresses for their chance of survival. These stresses can lead to disastrous crop production and limited distribution of crops geographically. The use of chemicals to address those stresses in agriculture is limited, while management strategies often fail to meet the needs of the industry. Therefore, novel techniques are needed to control plant diseases and to maintain food security. RCF1 has been found to encode an RNA helicase containing DEAD-box domains and loss-of-function mutation of this gene increases the sensitivity of plants to cold stress. In this study, a single mutant generated in RCF1 was used to investigate the function of the RCF1 gene in pathogen infection stress (i.e., Pst DC3000 and B. cinerea). RFC1 was found to confer resistance to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) but negatively affect Botrytis cinerea (B. cinerea) infection by repressing SNI1 pathways and JA signaling while activating SA pathways and its receptor NPR1. The generated rcf1 allelic mutant (name: 407) showed sensitivities to bacteria pathogen (Pst DC3000) but tolerance to fungus infection (B. cinerea), and SNI1 was highly upregulated in it. Free and conjugated Salicylic Acid (SA) contents were much lower in the allelic mutant 407 than in complementary materials and is sensitive to Pst DC3000 infection, and NPR1 depending on SA biosynthetic genes were down-regulated in it. Our preliminary results reveal that RCF1 confers resistance to Pst DC3000 by SA and NPR1 dependence manner but blocks SNI1 and JA pathways.
期刊：
Computational and Structural Biotechnology Journal,2023年21:168-175 ISSN：2001-0370
通讯作者：
Xiongfeng Dai<&wdkj&>Manlu Zhu
作者机构：
[Han, Fei; Dai, Xiongfeng; Wang, Yanling; Zhu, Manlu; Wang, Qian; Mu, Haoyan] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.
通讯机构：
[Xiongfeng Dai; Manlu Zhu] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, Hubei Province, China<&wdkj&>Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, Hubei Province, China
关键词：
(p)ppGpp;Growth control;Resource allocation;Bacteria;Plant and green algaes
摘要：
Rapid growth and survival are two key traits that enable bacterial cells to thrive in their natural habitat. The guanosine tetraphosphate and pentaphosphate [(p)ppGpp], also known as "magic spot", is a key sec-ond messenger inside bacterial cells as well as chloroplasts of plants and green algae. (p)ppGpp not only controls various stages of central dogma processes (replication, transcription, ribosome maturation and translation) and central metabolism but also regulates various physiological processes such as pathogen-esis, persistence, motility and competence. Under extreme conditions such as nutrient starvation, (p) ppGpp-mediated stringent response is crucial for the survival of bacterial cells. This mini-review high-lights some of the very recent progress on the key role of (p)ppGpp in bacterial growth control in light of cellular resource allocation and cell size regulation. We also briefly discuss some recent functional insights into the role of (p)ppGpp in plants and green algae from the angle of growth and development and further discuss several important open directions for future studies.(c) 2022 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open access article under the CC BY-NC-ND license (http://creative-commons.org/licenses/by-nc-nd/4.0/).
通讯作者：
Hong Yang<&wdkj&>Hong Yang Hong Yang Hong Yang
作者机构：
[Yang, Hong; Li, Xin-Yu; Mei, Cheng; Wulamu, Dilinuer] Cent China Normal Univ, Inst Entomol, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhan, Shuai; Luo, Xing-Yu; Huang, Yong-Ping] Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, CAS Key Lab Insect Dev & Evolutionary Biol, Inst Plant Physiol & Ecol,Shanghai Inst Biol Sci, Shanghai, Peoples R China.
通讯机构：
[Hong Yang; Hong Yang Hong Yang Hong Yang] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Institute of Entomology, School of Life Sciences, Central China Normal University, Wuhan, China
关键词：
bacterial isolates;germ-free BSF larvae;growth and development;Hermetia illucens;promotive effects
摘要：
Graphical Abstract Analysis of the intestinal bacterial community composition of Hermetia illucens (black soldier fly, BSF) larvae (BSFL) with pyrosequencing showed that the dynamics of bacterial community composition among different larval instars were striking at the genus level. Klebsiella, Clostridium, Providencia, Dysgonomonas, Dysgonomonas, and Providencia were the relatively most abundant bacterial groups in the 1st‐ to 6th‐instar BSFL, respectively. Providencia, Citrobacter, Klebsiella, Ochrobactrum, and Dysgonomonas promoted the growth and development of BSF significantly compared with the germ‐free control by increasing the weight gain of larvae and pupae, as well as the prepupae rate and eclosion rate. In addition, Citrobacter, Klebsiella, and Providencia could shorten the life cycle of BSF significantly. Abstract Black soldier fly (BSF), Hermetia illucens (Diptera: Stratiomyidae), is a prominent insect for the bioconversion of various organic wastes. As a saprotrophic insect, the BSF inhabits microbe‐rich environments. However, the influences of the intestinal microorganisms on BSF growth and development are not very clear. In this study, the dynamics of the intestinal bacterial community of BSF larvae (BSFL) were analyzed using pyrosequencing. Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria were the most prevalent bacterial phyla in the intestines of all larval instars. The dynamic changes in bacterial community compositions among different larval instars were striking at the genus level. Klebsiella, Clostridium, Providencia, and Dysgonomonas were the relatively most abundant bacteria in the 1st‐ to 4th‐instar BSFL, respectively. Dysgonomonas and Providencia also dominated the 5th‐ and 6th‐instar larvae, at ratios of 31.1% and 47.2%, respectively. In total, 148 bacterial strains affiliated with 20 genera were isolated on different media under aerobic and anaerobic conditions. Among them, 6 bacteria, BSF1–BSF6, were selected for further study. The inoculation of the 6 isolates independently into germ‐free BSFL feeding on an artificial diet showed that all the bacteria, except BSF4, significantly promoted BSF growth and development compared with the germ‐free control. Citrobacter, Dysgonomonas, Klebsiella, Ochrobactrum, and Providencia promoted BSF development significantly by increasing the weight gains of larvae and pupae, as well as increasing the prepupae and eclosion rates. In addition, Citrobacter, Klebsiella and Providencia shortened the BSF life cycle significantly. The results illustrate the promotive effects of intestinal bacteria on BSF growth and development.
期刊：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2023年140:104620 ISSN：0145-305X
通讯作者：
Xu-Sheng Liu
作者机构：
[Wang, Gui-Jie; Liu, Xu-Sheng; Wang, Jia-Lin] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Peoples R China.;[Liu, Xu-Sheng] Cent China Normal Univ, Sch Life Sci, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Xu-Sheng Liu] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 152 Luoyu Road, Wuhan, 430079, China
期刊：
JOURNAL OF PROTEOME RESEARCH,2023年22(9):2814-2826 ISSN：1535-3893
通讯作者：
Wan, CH
作者机构：
[Wan, Cuihong; Wan, CH; Ding, Yuhe; Gu, Jingyu; Peng, Zhao; Yan, Jiahao; Qin, Lu] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Wan, Cuihong; Wan, CH; Ding, Yuhe; Gu, Jingyu; Peng, Zhao; Yan, Jiahao; Qin, Lu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Wan, CH ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
zebrafish;early life stage;development;maternal-to-zygotic transition;mass spectrometry
摘要：
The early development of zebrafish (Danio rerio) is a complex and dynamic physiological process involving cell division, differentiation, and movement. Currently, the genome and transcriptome techniques have been widely used to study the embryonic development of zebrafish. However, the research of proteomics based on proteins that directly execute functions is relatively vacant. In this work, we apply label-free quantitative proteomics to explore protein profiling during zebrafish's embryogenesis, and a total of 5961 proteins were identified at 10 stages of zebrafish's early development. The identified proteins were divided into 11 modules according to weighted gene coexpression network analysis (WGCNA), and the characteristics between modules were significantly different. For example, mitochondria-related functions enriched the early development of zebrafish. Primordial germ cell-related proteins were identified at the 4-cell stage, while the eye development event is dominated at 5 days post fertilization (dpf). By combining with published transcriptomics data, we discovered some proteins that may be involved in activating zygotic genes. Meanwhile, 137 novel proteins were identified. This study comprehensively analyzed the dynamic processes in the embryonic development of zebrafish from the perspective of proteomics. It provided solid data support for further understanding of the molecular mechanism of its development.
通讯机构：
[Xiong, YZ; Lenhard, M ] U;[Xiong, YZ ] C;Cent China Normal Univ, Sch Life Sci, Luoyu Ave 152, Wuhan 430079, Peoples R China.;Univ Potsdam, Inst Biochem & Biol, Karl Liebknecht Str 24-25, D-14476 Potsdam, Germany.
关键词：
benzaldehyde;β-ocimene;Brassicaceae;Capsella;floral scent;pollinator attraction;quasi-isogenic line
摘要：
PREMISE OF THE STUDY: Floral scent, usually consisting of multiple compounds, is a complex trait and its role in pollinator attraction has received increasing attention. However, it is difficult to disentangle the effect of individual floral scent compounds due to the complexity of isolating the effect of single compounds by traditional methods. METHODS: Using available quasi-isogenic lines (qILs) generated as part of the original mapping of two floral scent volatile-related loci, CNL1 (benzaldehyde) and TPS2 (β-ocimene), in Capsella, we have generated four genotypes that should only systematically differ in these two compounds. Plants of the four genotypes were introduced into a common garden outside the natural range of C. rubella or C. grandiflora, with individuals of a self-compatible C. grandiflora line as pollen donors, whose different genetic background facilitates the detection of outcrossing events. Visitors to flowers of all five genotypes were compared, and the seeds set during the common-garden period were collected for high-throughput amplicon-based sequencing to estimate their outcrossing rates. KEY RESULTS: Benzaldehyde and β-ocimene emissions were detected in the floral scent of corresponding genotypes. While some pollinator groups showed specific visitation preferences depending on scent compounds, the outcrossing rates in seeds did not vary among the four scent-manipulated genotypes. CONCLUSIONS: The successful construction of scent-manipulated Capsella materials using qILs provides a powerful system to study the ecological effects of individual floral scent compounds under largely natural environments. In Capsella, individual BAld and β-ocimene emission may act as attractants for different types of pollinators. This article is protected by copyright. All rights reserved.
作者机构：
[Fang, Yiwei; Peng, Meilin; Zhao, Kai; Liu, Chunyan] Huazhong Univ Sci & Technol, Inst Reprod Hlth, Tongji Med Coll, Wuhan 430030, Peoples R China.;[Zhang, Zheng; Yi, Guilin] Wuhan Prevent & Treatment Ctr Occupat Dis, Wuhan 430015, Peoples R China.;[Zhang, Zheng] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Zhao, Yun; Sun, Guoqiang; Yang, Hui] Huazhong Univ Sci & Technol, Maternal & Child Hlth Hosp Hubei Prov, Tongji Med Coll, Dept Obstet, Wuhan 430070, Peoples R China.;[Zhao, Kai] Henan Inst Reprod Hlth Sci & Technol, NHC Key Lab Birth Defects Prevent, Zhengzhou 450000, Peoples R China.
通讯机构：
[Guilin Yi] W;[Kai Zhao] I;[Hui Yang] D;Department of Obstetrics, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430070, China<&wdkj&>Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China<&wdkj&>NHC Key Laboratory of Birth Defects Prevention, Henan Institute of Reproduction Health Science and Technology, Zhengzhou, 450000, China<&wdkj&>Wuhan Prevention and Treatment Center for Occupational Diseases, Wuhan, 430015, China
作者机构：
[Wan, Cuihong; Wan, CH; Zhang, Zheng; Li, Shengjie; Pan, Ni; Peng, Die; Wang, Shaohui] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Wan, Cuihong; Wan, CH; Zhang, Zheng; Li, Shengjie; Pan, Ni; Peng, Die; Wang, Shaohui] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Wan, CH ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
摘要：
Short open reading frame-encoded peptides (SEPs) are generally 2-100 amino acids in length and participate in various biological processes of the organism. The brain is the central hub of life activities, where different regions perform distinct functions. To characterize SEPs in brain regions, we analyzed SEPs in five mouse brain areas, including hippocampus, frontal cortex, temporal cortex, occipital cortex, and parietal cortex, with mass spectrometry-based proteomics. We obtained 1,095 proteins with less than 100 amino acids and identified 373 SEPs. Approximately 83% of these SEPs are reported for the first time. Half of them are encoded by ncRNA, and nearly one-third can find orthology across species. Specific SEPs were identified in each brain region. For example, IP_1018875 was identified in the frontal cortex, possibly related to autophagy and neuronal signaling. These results enrich the proteome of the mouse brain and help facilitate subsequent studies on the function of SEPs.
