摘要：
Engleromyces goetzei Henn (EgH) is a natural fungus that has been used as a traditional edible and medicine for long time in Southwest China. Our study found EgH aqueous extract (EgH‐AE) has very strong activities on antioxidant and anti‐inflammation. At the same time, we also found EgH‐AE has good biocompatibility and cell protective function, so it is biosafe, EgH‐AE may have the prospect of developing into functional beverage. Abstract High body mass index (high BMI, obesity) is a serious public health problem, and “obesity‐induced oxidative stress, inflammation, and cancer” have become modern epidemic diseases. We carried out this study to explore a functional beverage that may protect against obesity‐induced diseases. The Engleromyces goetzei Henn herbal tea is such a candidate. For this study, we carried out LC–MS analysis of E. goetzei Henn aqueous extract (EgH‐AE); then used the Caco‐2 cell line for the model cells and treated the cells with t‐BHP to form an oxidative stress system. An MTT assay was used for testing the biocompatibility and cytoprotective effects; reactive oxygen species and malondialdehyde determination was used for evaluating the antioxidative stress effect; TNF‐α and IL‐1β were used for observing the anti‐inflammatory effect, and 8‐OHdG for monitoring anticancer activity. The results of this study demonstrate that the EgH‐AE has very good biocompatibility with the Caco‐2 cell line and has good cytoprotective, antioxidant, anti‐inflammatory, and anticancer properties. It is clear that EgH‐AE, a kind of ancient herbal tea, may be used to develop a functional beverage that can be given to people with a high BMI to protect against obesity‐induced diseases.
作者机构：
[Luo, Jingwen; Li, Mengyun; Chen, Feng; Xu, Wenliang; Gao, Yanan; Guo, Yanjun; Qiao, Mengfei; Xu, WL] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.;[Guo, Yanjun] Xinjiang Normal Univ, Coll Life Sci, Urumqi, Peoples R China.;[Persson, Staffan] Univ Copenhagen, Copenhagen Plant Sci Ctr, Frederiksberg, Denmark.;[Persson, Staffan] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Joint Int Res Lab Metab & Dev Sci, Shanghai, Peoples R China.;[Zeng, Wei; Zeng, W] Taizhou Univ, Sch Life Sci, Taizhou, Peoples R China.
通讯机构：
[Zeng, W ] T;[Xu, WL ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.;Taizhou Univ, Sch Life Sci, Taizhou, Peoples R China.
摘要：
Phosphorus (P) is a crucial macronutrient for plant growth, development, and reproduction. The effects of low P (LP) stress on leaf senescence and the role of PHR1 in LP-induced leaf senescence are still unknown. Here, we report that PHR1 plays a crucial role in LP-induced leaf senescence, showing delayed leaf senescence in phr1 mutant and accelerated leaf senescence in 35S:PHR1 transgenic Arabidopsis under LP stress. The transcriptional profiles indicate that 763 differentially expressed SAGs (DE-SAGs) were upregulated and 134 DE-SAGs were downregulated by LP stress. Of the 405 DE-SAGs regulated by PHR1, 27 DE-SAGs were involved in P metabolism and transport. PHR1 could bind to the promoters of six DE-SAGs (RNS1, PAP17, SAG113, NPC5, PLD zeta 2, and Pht1;5), and modulate them in LP-induced senescing leaves. The analysis of RNA content, phospholipase activity, acid phosphatase activity, total P and phosphate content also revealed that PHR1 promotes P liberation from senescing leaves and transport to young tissues under LP stress. Our results indicated that PHR1 is one of the crucial modulators for P recycling and redistribution under LP stress, and the drastic decline of P level is at least one of the causes of early senescence in P-deficient leaves. In Arabidopsis, PHR1 is the crucial regulator in low phosphate-induced leaf senescence by modulating P recycling and redistribution.
作者机构：
[Wei, Fuwen; Hu, Yibo] Chinese Acad Sci, CAS Key Lab Anim Ecol & Conservat Biol, Beijing 100101, Peoples R China.;[Wei, Fuwen; Yang, Guang] Southern Marine Sci & Engn Guangdong Lab Guangzhou, Guangzhou 511458, Peoples R China.;[Yu, Li; Wang, Xiaoping] Yunnan Univ, Sch Life Sci, State Key Lab Conservat & Utilizat Bioresources Yu, Kunming 650091, Peoples R China.;[Guo, Yalong; Xu, Yongchao] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolut Bot, Beijing 100093, Peoples R China.;[Shi, Peng; Yang, Hui] Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650201, Peoples R China.
通讯机构：
[Yibo Hu; Fuwen Wei] C;[Li Yu; Yalong Guo; Peng Shi; Guang Yang; Suhua Shi] S;[Shuangquan Huang] I;CAS Key Lab of Animal Ecology and Conservation Biology, Chinese Academy of Sciences, Beijing, 100101, China.;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming, 650091, China.
摘要：
Wild animals and plants have developed a variety of adaptive traits driven by adaptive evolution, an important strategy for species survival and persistence. Uncovering the molecular mechanisms of adaptive evolution is the key to understanding species diversification, phenotypic convergence, and inter-species interaction. As the genome sequences of more and more non-model organisms are becoming available, the focus of studies on molecular mechanisms of adaptive evolution has shifted from the candidate gene method to genetic mapping based on genome-wide scanning. In this study, we reviewed the latest research advances in wild animals and plants, focusing on adaptive traits, convergent evolution, and coevolution. Firstly, we focused on the adaptive evolution of morphological, behavioral, and physiological traits. Secondly, we reviewed the phenotypic convergences of life history traits and responding to environmental pressures, and the underlying molecular convergence mechanisms. Thirdly, we summarized the advances of coevolution, including the four main types: mutualism, parasitism, predation and competition. Overall, these latest advances greatly increase our understanding of the underlying molecular mechanisms for diverse adaptive traits and species interaction, demonstrating that the development of evolutionary biology has been greatly accelerated by multi-omics technologies. Finally, we highlighted the emerging trends and future prospects around the above three aspects of adaptive evolution.
作者：
Cao, Ya Ya;Chen, Yuan Yuan;Wang, Ming Shu;Tong, Jing Jing;Xu, Meng;...
期刊：
Redox Biology,2023年63:102751 ISSN：2213-2317
通讯作者：
Zhang, Dan;Yang, GF
作者机构：
[Chen, Yuan Yuan; Zhang, Wen Lin; Yang, Guang Fu; Lin, Hong Yan; Mei, Long Can; Wang, Ming Shu; Dong, Jin; Zhang, Dan; Zhang, D; Cao, Ya Ya; Huang, Wei; Qin, Yu Xuan; Zhao, Chi] Cent China Normal Univ, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.;[Tong, Jing Jing; Xu, Meng] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.;[Chen, Yuan Yuan] Hunan Univ, Inst Chem Biol & Nanomed, Coll Chem & Chem Engn, Changsha 410082, Peoples R China.
通讯机构：
[Zhang, D; Yang, GF ] C;Cent China Normal Univ, Int Joint Res Ctr Intelligent Biosensor Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.
关键词：
Catalase inhibitors;NADPH-Binding site;Ferroptosis;Castration-resistant prostate cancer
摘要：
Catalase (CAT) is an important antioxidant enzyme that breaks down H(2)O(2) into water and oxygen. Inhibitor-modulating CAT activity in cancer cells is emerging as a potential anticancer strategy. However, the discovery of CAT inhibitors towards the heme active center located at the bottom of long and narrow channel has made little progress. Therefore, targeting new binding site is of great importance for the development of efficient CAT inhibitors. Here, the first NADPH-binding site inhibitor of CAT, BT-Br, was designed and synthesized successfully. The cocrystal structure of BT-Br-bound CAT complex was determined with a resolution of 2.2Å (PDB ID:8HID), which showed clearly that BT-Br bound at the NADPH-binding site. Furthermore, BT-Br was demonstrated to induce ferroptosis in castration-resistant prostate cancer (CRPC) DU145cells and eventually reduce CRPC tumors in vivo effectively. The work indicates that CAT has potential as a novel target for CRPC therapy based on ferroptosis inducing.
通讯机构：
[Haobin Zhao] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China
摘要：
Mep50 as a partner promotes the activity and substrate affinity of Prmt5. Prmt5 and Mep50 function together in multiple bioprocesses of the cells. Both Prmt5 and Mep50 are necessary for maintenance of the stem cells and are indispensable in the embryogenesis in the mammals. However, the role of Mep50 is rarely studied in fish. This study was to investigate the role of Mep50 in embryonic development of medaka. Medaka mep50 was mutated by genomic editing with CRISPR-Cas9 technology. Two mutants with a deletion of 22 and 46 bp separately in mep50 caused premature stopping of translation. The homozygotes of these mutant fish were obtained by self-crossing of the heterozygotes. These homozygotic mutants could reproduce embryos but the offspring were not viable. The apoptotic cells were significantly more in the mutant embryos than that in the wild type indicated by TUNEL assay. Quantitative RT-PCR showed that the expression of oct4 and sox2 were significantly decreased, but p53 was increased in the mutant embryos. These results suggest that disruption of mep50 severely interferes with embryogenesis and mep50 is necessary for embryonic development by maintaining stem cells and repression of apoptosis in medaka.
作者：
Luo, Jinhong*;Lu, Manman;Luo, Jie;Moss, Cynthia F.
期刊：
JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY,2023年209(1):203-214 ISSN：0340-7594
通讯作者：
Luo, Jinhong;Moss, Cynthia F
作者机构：
[Lu, Manman; Luo, Jie; Luo, Jinhong] Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.;[Moss, Cynthia F.] Johns Hopkins Univ, Dept Psychol & Brain Sci, Baltimore, MD 21218 USA.
通讯机构：
[Luo, Jinhong] I;[Moss, Cynthia F] D;Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China.;Department of Psychological and Brain Sciences, Johns Hopkins University, Baltimore, MD, 21218, USA. cynthia.
摘要：
Diverse animal taxa are capable of rapidly modifying vocalizations to mitigate interference from environmental noise. Echolocating bats, for example, must frequently perform sonar tasks in the presence of interfering sounds. Numerous studies have documented sound production flexibility in echolocating bats; however, it remains unknown whether noise-induced vocal modifications (NIVMs) mitigate interference effects on echoes or calls. In this study, we leverage echo level compensation behavior of echolocating bats to answer this question. Using a microphone array, we recorded echolocation calls of Hipposideros pratti trained to approach and land on a perch in the laboratory under quiet and noise conditions. We found that H. pratti exhibited echo level compensation behavior during approaching flights, which depended critically on distance to the landing perch. Broadcast noise delayed and affected the rate of echo level compensation in H. pratti. Moreover, H. pratti increased vocalization amplitude, i.e., exhibited the Lombard effect, while also adjusting call duration and bandwidth with increasing noise levels. Quantitative analyses of the data show that H. pratti relies on echo feedback, not vocal feedback, to adjust signals in the presence of noise. These findings provide compelling evidence that NIVMs in echolocating animals and non-echolocating animals operate through different mechanisms.
作者机构：
[Fu, Ziying; Fan, Zihui; Cui, Zhongdan; Chen, Qicai; Zhang, Guimin] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Yang, Lijian; Jia, Ya] Cent China Normal Univ, Coll Phys Sci & Technol, Wuhan 430079, Peoples R China.
通讯机构：
[Ziying Fu] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, Hubei 430079, China
摘要：
The Lombard effect, ref erring to an involuntary rise in vocal intensity, is a widespread vertebrate mecha-nism that aims to maintain signal efficiency in response to ambient noise. Previous studies showed that the Lombard effect could be sufficiently implemented at subcortical levels and operated by continuously monitoring background noise, requiring some subcortical auditory sensitive neurons to have continuous responses to background noise. However, such neurons have not been well characterized. The inferior colliculus (IC) is a major auditory integration center under the auditory cortex and provides projections to the putative vocal pattern generator in the brainstem. Thus, it is reasonable to speculate that the IC is a likely auditory nucleus candidate having background noise responding neurons (BNR neurons). In the present study, we isolated 183 sound-sensitive IC neurons in a constant frequency-frequency mod-ulation bat, Hipposideros pratti, and found that around 19% of these IC neurons are BNR neurons when stimulated with 70 dB SPL background white noise. Their firing rates in response to noise increased with increasing noise intensity and could be suppressed by sound stimulation. Furthermore, compared to neu-rons with similar best frequencies, the BNR neurons had smaller Q 10-dB values and lower noise-induced minimal threshold change, indicating that BNR neurons received fewer inhibitory inputs. These results suggested that the BNR neurons are ideal candidates for collecting information about background noise. We proposed that the BNR neurons synapsed with neurons in vocal-pattern-generating networks in the brainstem and initiated the Lombard effect by a feed-forward loop. (c) 2023 Elsevier B.V. All rights reserved.
摘要：
Algicidal bacteria can inhibit the growth of algae or lyse algal cells, thus playing roles in shaping aquatic microbial communities and maintaining the functions of aquatic ecosystems. Nevertheless, our understanding of their diversities and distributions remains limited. In this study, we collected water samples from 17 freshwater sites in 14 cities in China and screened a total of 77 algicidal bacterial strains using several prokaryotic cyanobacteria and eukaryotic algae as target strains. According to their target-specificities, these strains were classified into three subgroups, cyanobacterial algicidal bacteria, algal algicidal bacteria, and broad-target algicidal bacteria, each displaying distinctive compositions and geographical distribution patterns. They are assigned to Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes bacterial phyla, of which Pseudomonas and Bacillus are the most abundant gram-negative and gram-positive genus, respectively. A number of bacterial strains, such as Inhella inkyongensis and Massilia eburnean, are suggested as new algicidal bacteria. The diverse taxonomies, algal-inhibiting abilities and distributions of these isolates have suggested that there are rich algicidal bacterial resources in these aquatic environments. Our results provide new microbial resources for algal-bacterial interaction studies, and shed new insights into how algicidal bacteria can be used in the control of harmful algal blooms, as well as in algal biotechnology.
期刊：
Annals of Botany,2023年132(6):viii-x ISSN：0305-7364
通讯作者：
Huang, SQ
作者机构：
[Huang, Shuang-Quan; Huang, SQ] Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.
通讯机构：
[Huang, SQ ] C;Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.
关键词：
Enantiostyly;floral sexual polymorphism;geitonogamous self-pollination;tracing pollen movement
摘要：
This article comments on:Steven D. Johnson, Jeremy J. Midgley and Nicola Illing. The enantiostylous floral polymorphism of Barberetta aurea (Haemodoraceae) faci
期刊：
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION,2023年107(1):228-237 ISSN：0931-2439
通讯作者：
Wan, LL;Ai, H
作者机构：
[Feng, Ke; Chen, Yan; Lu, Jianjian] Wuhan Polytech Univ, Sch Mech Engn, Wuhan, Peoples R China.;[Wan, Lili] Wuhan Polytech Univ, Sch Elect & Elect Engn, Wuhan 430048, Peoples R China.;[Ai, Hui] Cent China Normal Univ, Sch Life Sci, Wuhan, Peoples R China.
通讯机构：
[Ai, H ] C;[Wan, LL ] W;Wuhan Polytech Univ, Sch Elect & Elect Engn, Wuhan 430048, Peoples R China.;Cent China Normal Univ, Sch Life Sci, Wuhan, Peoples R China.
摘要：
The chicken is rich in various proteins, fatty acids, polysaccharides, trace elements, and other human essential nutrients that contribute to its high nutritional value. In this study, the expression levels of nutrition-related genes (acetyl-CoA acyltransferase, ACAA) of native chicken breeds were investigated. The level of GgalACAA1-2 transcripts expression in the liver of chicken was significantly higher than that of muscle and heart. Moreover, three protein extracts were isolated from the muscle, heart, and liver tissues from the chicken, and their nutritional function was evaluated in the present study. These protein extracts had excellent DPPH and hydroxyl radical scavenging capacities and exhibited significant superoxide anion scavenging ability. Moreover, the protein extracts of muscle tissue showed an important mouse splenocyte proliferation activity and could be used as an immunomodulator of natural origin. In addition, this report presented an automatic visual inspection of chicken viscera using the active contour algorithms and the image processing method for eviscerating by the parallel robot. The recognition and positioning rate of chicken viscera obtained by the proposed method could reach 96.45%. These methods provided basic data for automated poultry slaughter and segmentation, avoiding unnecessary health risks by a pathogenic microorganism, such as avian influenza, Newcastle disease virus, and coronavirus. Moreover, the internal organs of the chicken could be fully harvested by the image segmentation of automatic evisceration, which also facilitated the processing value of these internal organs as by-products of poultry.
摘要：
Studies have shown that exposure to either microplastics (MPs) or di-(2-ethylhexyl) phthalic acid (DEHP) alone can cause neurotoxicity in animals, but it remains uncertain whether and to what extent co-exposure to these two substances, which often occur together in reality, can also induce neurotoxicity. This study aimed to investigate the neurotoxicity and molecular mechanisms of combined exposure to DEHP and polypropylene microplastics (synthetic PP-MPs were used), the microplastics most commonly encountered by young children, in immature mice. The results showed that exposure to PP-MPs and/or DEHP did cause neurotoxic effects in immature mice, including induction of neurocognitive and memory deficits, damage to the CA3 region of the hippocampus, increased oxidative stress, and decreased AChE activity in the brain. The severity of the neurotoxicity increased with increasing concentrations of PP-MPs, combined exposure to PP-MPs and DEHP exhibited additive or synergistic effects. Transcriptomic analyses revealed that the PP-MPs and/or DEHP exposure altered the expression profiles of gene clusters involved in the stress response, and in protein processing in endoplasmic reticulum. Quantitative analyses further indicated that PP-MPs and/or DEHP exposure inhibited the activity of the heat shock response mediated by heat shock transcription factor 1, while chronically activated the unfolded protein response, consequently inducing neurotoxicity through neuronal apoptosis and neuroinflammation in the immature mice. As a pioneer study to highlight the neurotoxicity induced by combined exposure to PP-MPs and DEHP in immature mice, this research provides new insights into mitigating the health risks of PP-MPs and DEHP exposure in young children.
作者机构：
[Qi, Yong; Wang, Shan-Shan; Li, Lu-Lin] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.
通讯机构：
[Lu-Lin Li] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China
关键词：
AcMNPV;IE1;baculovirus;late gene expression
摘要：
The late gene expression of baculovirus has been known to depend on the virus-encoded RNA polymerase, which consists of four subunits. The immediate-early gene ie1 was found to be required for viral early gene expression, late gene expression, and DNA replication. Early and late gene expressions of baculoviruses have been known to rely on host RNA polymerase II and a virus-encoded RNA polymerase, separately. In this study, we found that Autographa californica multiple nucleopolyhedrovirus (AcMNPV) recombinant bacmids with the individual RNA polymerase subunit genes deleted could support low levels of expression of a reporter gene under the control of the promoter of a typical late gene, vp39, in transfected Sf9 cells. Through multistep subcloning of a genomic library of the virus and transient expression assay analysis, ie1 was identified to be the only viral gene that was responsible for activation of late gene expression in the absence of the viral RNA polymerase. Furthermore, IE1 was found to be capable of activating reporter gene expression from the promoters of additional late genes polh, p6.9, odv-e18, odv-e25, and gp41, independent of any additional viral factors. Deletion of ie1 from the virus genome eliminated late gene expression. The IE1-activated late gene expression was enhanced by the viral hr4b. It was shown to be insensitive to inhibition of alpha-amanitin and did not appear to have stable transcription start sites. It is proposed that IE1 may serve to recruit newly synthesized viral RNA polymerase to viral DNA by activating low levels of pretranscription of the late genes to create an appropriate DNA conformation.IMPORTANCE The late gene expression of baculovirus has been known to depend on the virus-encoded RNA polymerase, which consists of four subunits. The immediate-early gene ie1 was found to be required for viral early gene expression, late gene expression, and DNA replication. How it functions in late gene expression remains unclear. In this study, we found that AcMNPV IE1 could activate low levels of gene expression from late gene promoters independently of any additional viral factors, with nonspecific transcription start sites. This new finding will shed light on the role of IE1 in the regulation of late gene expression and the understanding of the mechanism of late gene transcription initiation.
摘要：
Successful phytoremediation of acidic metal-contaminated mine tailings requires amendments to condition tailings properties prior to plant establishment. This conditioning process is complex and includes multiple changes in tailings bio-physico-chemical properties. The objective of this project is to identify relationships between tailings properties, the soil microbiome, and plant stress response genes during growth of Atriplex lentiformis in compost-amended (10 %, 15 %, 20 % w/w) mine tailings. Analyses include RNA-Seq for plant root gene expression, 16S rRNA amplicon sequencing for bacterial/archaeal communities, metal concentrations in both tailings and plant organs, and phenotypic measures of plant stress. Zn accumulation in A. lentiformis leaves varied with compost levels and was the highest in the intermediate treatment (15 %, TC15). Microbial analysis identified Alicyclobacillus, Hydrotalea, and Pseudolabrys taxa with the highest relative abundance in TC15, and these taxa were strongly associated with Zn accumulation. Furthermore, we identified 190 root genes with significant gene expression changes. These root genes were associated with different pathways including, abscisic acid and auxin signaling, defense responses, ion channels, metal ion binding, oxidative stress, transcription regulation, and transmembrane transport. However, root gene expression changes were not driven by the increasing levels of compost. For example, there were 15 genes that were up-regulated in TC15, whereas 106 genes were down-regulated in TC15. The variables analyzed explained 86 % of the variance in Zn accumulation in A. lentiformis leaves. Importantly, Zn accumulation was driven by Zn shoot concentrations, leaf stress symptoms, plant root genes, and microbial taxa. Therefore, our results suggest there are strong plant-microbiome associations that drive Zn accumulation in A. lentiformis and different plant gene pathways are involved in alleviating varying levels of metal stress. Future work is needed to gain a mechanistic understanding of these plant-microbiome interactions to optimize phytoremediation strategies as they will govern the success or failure of the revegetation process.
期刊：
JOURNAL OF PROTEOME RESEARCH,2023年22(4):1172-1180 ISSN：1535-3893
通讯作者：
Cuihong Wan
作者机构：
[Wang, Yi; Wan, Cuihong; Zhang, Zheng; Peng, Mingbo; Pan, Ni] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Wang, Yi; Wan, Cuihong; Zhang, Zheng; Peng, Mingbo; Pan, Ni] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Tian, Tao; Pan, Zhenwei; Zhao, Xinbo] Minist Educ, Dept Pharmacol, Key Lab Cardiovasc Med Res, Harbin 150081, Heilongjiang, Peoples R China.;[Tian, Tao; Pan, Zhenwei; Zhao, Xinbo] Harbin Med Univ, State Prov Key Labs Biomed Pharmaceut China, Harbin 150081, Heilongjiang, Peoples R China.
通讯机构：
[Cuihong Wan] S;School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, Hubei 430079, People’s Republic of China
摘要：
The incidence rate of atrial fibrillation (AF) has stayed at a high level in recent years. Despite the intensive efforts to study the pathologic changes of AF, the molecular mechanism of disease development remains unclarified. Microproteins are ribosomally translated gene products from small open reading frames (sORFs) and are found to play crucial biological functions, while remain rare attention and indistinct in AF study. In this work, we recruited 65 AF patients and 65 healthy subjects for microproteomic profiling. By differential analysis and cross-validation between independent datasets, a total of 4 microproteins were identified as significantly different, including 3 annotated ones and 1 novel one. Additionally, we established a diagnostic model with either microproteins or global proteins by machine learning methods and found the model with microproteins achieved comparable and excellent performance as that with global proteins. Our results confirmed the abnormal expression of microproteins in AF and may provide new perspectives on the mechanism study of AF.
摘要：
Increasing attention has been paid to the potential impact of microplastics (MPs) pollution on human health. MPs and phthalates coexist in the environment, however, the effects of exposure to MPs alone or to a combination of di-(2-ethylhexyl) phthalate (DEHP) and MPs on allergic asthma are unclear. This study investigates the effects of exposure to polystyrene microplastics (PS-MPs) or co-exposure with DEHP, on allergic asthma, and the underlying molecular mechanisms. We established an allergic asthma model using ovalbumin, and mice were exposed to PS-MPs (5mg/kg bw/day) alone, or combined with DEHP (0.5, 5mg/kg bw/day), for 28 days. The results showed that in the presence of ovalbumin (OVA) sensitization, exposure to PS-MPs alone slightly affected airway inflammation, and airway hyperresponsiveness, while co-exposure to PS-MPs and DEHP caused more significant damage. Co-exposure also induced more oxidative stress and Th2 immune responses, and activation of the TRPA1 and p38 MAPK pathways. The aggravation of asthmatic symptoms induced by co-exposure to PS-MPs and DEHP were inhibited by blocking TRPA1 ion channel or p38 MAPK pathway. The results demonstrated that co-exposure to PS-MPs and DEHP exacerbates allergic asthma, by exacerbating oxidative stress and inflammatory responses, and activating the TRPA1-p38 MAPK pathway.
期刊：
Landscape and Urban Planning,2023年233:104712 ISSN：0169-2046
通讯作者：
Hongyu Niu<&wdkj&>Hongmao Zhang
作者机构：
[Yue, Xingcheng; Chen, Zhiwen; Niu, Hongyu; Zhang, Hongmao; Zhao, Hengyue] Cent China Normal Univ, Inst Ecol & Evolut, Sch Life Sci, Wuhan 430079, Peoples R China.;[Rehling, Finn; Schabo, Dana G.; Niu, Hongyu; Farwig, Nina] Philipps Univ Marburg, Dept Biol, Conservat Ecol, D-35043 Marburg, Germany.;[Wang, Xiaorong] Hubei Acad Forestry, Wuhan 430075, Peoples R China.;[Niu, Hongyu; Zhang, Hongmao] 152 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Hongyu Niu; Hongmao Zhang] I;Institute of Ecology and Evolution, School of Life Sciences, Central China Normal University, Wuhan 430079, China<&wdkj&>Conservation Ecology, Department of Biology, Philipps-University of Marburg, Marburg 35043, Germany<&wdkj&>Institute of Ecology and Evolution, School of Life Sciences, Central China Normal University, Wuhan 430079, China
摘要：
Urban forests are highly fragmented in mega-cites, acting as islands in terms of preserving species diversity. To maintain the ecological services of urban forests, management measures such as reforestation have been implemented, which might have a long-term effect on biodiversity. To understand how fragmentation and reforestation affect the natural regeneration of urban forests, we investigated the relationship between forest area, isolation and seed dispersal mode and the diversity and composition of woody species at the tree, seedling, and sapling stages in 28 secondary forests of the mega-city of Wuhan, China. We found that the alpha diversity of woody species was positively correlated with forest area, while their beta diversity was negatively correlated with forest area. The beta diversity of nonanimal-dispersed species significantly correlated with isolation. Animal-dispersed plants had consistently a higher alpha diversity from trees to seedlings and saplings, while their beta diversity was lower than nonanimal-dispersed plants at the seedling and sapling stage. The community composition of woody plants in urban forests was largely congruent among the three life stages. However, only the communities of animal-dispersed plants were consistent across life stages in small or highly-isolated forest patches. The results show that the woody plant diversity of urban forests is largely similar to that expected by island theory. Animal-dispersed trees are more likely to regenerate successfully due to a more diverse set of species used in reforestation and their higher tolerance to urban forest fragmentation. More management measures for nonanimal-dispersed species, such as enriching and repeating reforestation, will be required to maintain their high biodiversity in urban forests.
