作者机构：
[Wan, Cuihong; Peng, Zhao] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Wan, Cuihong; Peng, Zhao] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Li, Jiaqiang; Jiang, Xingpeng] Cent China Normal Univ, Sch Comp, Wuhan 430079, Hubei, Peoples R China.;[Li, Jiaqiang; Jiang, Xingpeng] Cent China Normal Univ, Hubei Prov Key Lab Artificial Intelligence & Smart, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Xingpeng Jiang; Cuihong Wan] S;School of Computer, and Hubei Provincial Key Laboratory of Artificial Intelligence and Smart Learning, Central China Normal University , Wuhan 430079, Hubei , People's Republic of China<&wdkj&>School of Life Sciences, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University , Wuhan 430079, Hubei , People's Republic of China
摘要：
As one of the essential life forms in the biosphere, research on cyanobacteria has been growing remarkably for decades. Biological functions in organisms are often accomplished through protein-protein interactions (PPIs), which help to regulate interacting proteins or organize them into an integral machine. However, the study of PPIs in cyanobacteria falls far behind that in mammals and has not been integrated for ease of use. Thus, we built CyanoMapDB (http://www.cyanomapdb.msbio.pro/), a database providing cyanobacterial PPIs with experimental evidence, consisting of 52,304 PPIs among 6,789 proteins from 23 cyanobacterial species. We collected available data in UniProt, STRING, and IntAct, and mined numerous PPIs from co-fractionation MS data in cyanobacteria. The integrated data are accessible in CyanoMapDB (http://www.cyanomapdb.msbio.pro/), enabling users to easily query proteins of interest, investigate interacting proteins with evidence from different sources, and acquire a visual network of the target protein. We believe that CyanoMapDB will promote research involved with cyanobacteria and plants.
作者机构：
[Fu, Zi-Ying; Fu, ZY] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Fu, ZY ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.
期刊：
Journal of Hazardous Materials,2023年448:130990 ISSN：0304-3894
通讯作者：
Kaiyao Huang
作者机构：
[Zhang, Baolong; Long, Huan; Huang, Kaiyao; Deng, Xuan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China.;[Yu, Fei; Wang, Xun; Tang, Yuxin] Wuhan Univ Sci & Technol, Sch Urban Construct, Wuhan 430065, Hubei, Peoples R China.;[Peng, Zhao; Yao, Sheng] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Peng, Zhao; Yao, Sheng] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Huang, Kaiyao] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China.
通讯机构：
[Kaiyao Huang] K;Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei, China
关键词：
Algae;Ascorbate;Cd;IFR1;Ribo-seq
摘要：
Cadmium (Cd) is one of the most toxic pollutants found in aquatic ecosystems. Although gene expression in algae exposed to Cd has been studied at the transcriptional level, little is known about Cd impacts at the translational level. Ribosome profiling is a novel translatomics method that can directly monitor RNA translation in vivo. Here, we analyzed the translatome of the green alga Chlamydomonas reinhardtii following treatment with Cd to identify the cellular and physiological responses to Cd stress. Interestingly, we found that the cell morphology and cell wall structure were altered, and starch and high-electron-density particles accumulated in the cytoplasm. Several ATP-binding cassette transporters that responded to Cd exposure were identified. Redox homeostasis was adjusted to adapt to Cd toxicity, and GDP-L-galactose phosphorylase (VTC2), glutathione peroxidase (GPX5), and ascorbate were found to play important roles in maintaining reactive oxygen species homeostasis. Moreover, we found that the key enzyme of flavonoid metabolism, i.e., hydroxyisoflavone reductase (IFR1), is also involved in the detoxification of Cd. Thus, in this study, translatome and physiological analyses provided a complete picture of the molecular mechanisms of green algae cell responses to Cd.
期刊：
Journal of Molecular Medicine,2023年102(2):273-284 ISSN：0946-2716
通讯作者：
Huang, QY
作者机构：
[Cheng, Chen; Huang, Xinyao; Qin, Zixuan; Huang, Qingyang; Wang, Ya; Zhang, Qiongdan; Lu, Li] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Wang, Ya] Wenzhou Med Univ, Sch Lab Med & Life Sci, Key Lab Lab Med, Zhejiang Prov Key Lab Med Genet,Minist Educ, Wenzhou 325035, Zhejiang, Peoples R China.
通讯机构：
[Huang, QY ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
DNA methylation;E2F6;EN1;Enhancer;Noncoding SNP;Osteoporosis
摘要：
EN1 encodes a homeodomain-containing transcription factor and is a determinant of bone density and fracture. Previous powerful genome-wide association studies (GWASs) have identified multiple single-nucleotide polymorphisms (SNPs) near EN1 at 2q14.2 locus for osteoporosis, but the causal SNPs and functional mechanisms underlying these associations are poorly understood. The target genes regulated by the transcription factor EN1 are also unclear. In this study, we identified rs188303909, a functional CpG-SNP, as a causal SNP for osteoporosis at 2q14.2 through the integration of functional and epigenomic analyses. Functional experiments demonstrated that unmethylated rs188303909 acted as a strong allele-specific distal enhancer to regulate EN1 expression by modifying the binding of transcription factor E2F6, but rs188303909 methylation attenuated the active effect of E2F6 on EN1 expression. Importantly, transcription factor EN1 could differentially bind osteoporosis GWAS lead SNPs rs4869739-T and rs4355801-G to upregulate CCDC170 and COLEC10 expression, thus promoting bone formation. Our study provided a mechanistic insight into expression regulation of the osteoporosis susceptibility gene EN1, which could be a potential therapeutic target for osteoporosis precision medicine.
摘要：
Predator-prey interactions are important but difficult to study in the field. Therefore, laboratory studies are often used to examine the outcomes of predator-prey interactions. Previous laboratory studies have shown that moth hearing and ultrasound production can help prey avoid being eaten by bats. We report here that laboratory behavioural outcomes may not accurately reflect the outcomes of field bat-moth interactions. We tested the success rates of two bat species capturing moths with distinct anti-bat tactics using behavioural experiments. We compared the results with the dietary composition of field bats using next-generation DNA sequencing. Rhinolophus episcopus and Rhinolophus osgoodi had a lower rate of capture success when hunting for moths that produce anti-bat clicks than for silent eared moths and earless moths. Unexpectedly, the success rates of the bats capturing silent eared moths and earless moths did not differ significantly from each other. However, the field bats had a higher proportion of silent eared moths than that of earless moths and that of clicking moths in their diets. The difference between the proportions of silent eared moths and earless moths in the bat diets can be explained by the difference between their abundance in bat foraging habitats. These findings suggest that moth defensive tactics, bat countertactics and moth availability collectively shape the diets of insectivorous bats. This study illustrates the importance of using a combination of behavioural experiments and molecular genetic techniques to reveal the complex interactions between predators and prey in nature.
作者机构：
[Zhang, Liang; Sun, Hang; Deng, Tao; Chen, Jun-Tong; Sun, H; Deng, T; Huang, Xian-Han; Zhang, Xin-Jian; Kuang, Tian-Hui] Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Peoples R China.;[Chen, Jun-Tong; Zhang, Xin-Jian; Kuang, Tian-Hui] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Liden, Magnus] Uppsala Univ, Evolutionary Biol Ctr, Systemat Biol, S-75236 Uppsala, Sweden.;[Landis, Jacob B.] Cornell Univ, Sch Integrat Plant Sci, Sect Plant Biol, Ithaca, NY 14853 USA.;[Landis, Jacob B.] Cornell Univ, LH Bailey Hortorium, Ithaca, NY 14853 USA.
通讯机构：
[Sun, H ; Deng, T] C;Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Peoples R China.
关键词：
character evolution;Corydalis;genome skimming;Papaveraceae;phylogeny
摘要：
Phylogenetic analyses of Corydalis and related taxa, covering all 42 previously recognized sections and five independent “series”, combined with ancestral character reconstructions, uncovered previously unrecognized relationships and greatly improved our understanding of the evolution of the genus Corydalis. Abstract The genus Corydalis, with ca. 530 species, has long been considered taxonomically challenging because of its great variability. Previous molecular analyses, based on a few molecular markers and incomplete taxonomic sampling, were clearly inadequate to delimit sections and subgenera. We have performed phylogenetic analyses of Corydalis and related taxa, using 65 shared protein‐coding plastid genes from 313 accessions (including 280 samples of ca. 226 species of Corydalis) and 152 universal low‐copy nuclear genes from 296 accessions (including 271 samples of Corydalis) covering all 42 previously recognized sections and five independent “series”. Phylogenetic trees were inferred using Bayesian Inference and Maximum Likelihood. Eight selected morphological characters were estimated using ancestral state reconstructions. Results include: (i) of the three subgenera of Corydalis, two are fully supported by both the plastid and nuclear data; the third, subg. Cremnocapnos, is weakly supported by plastid DNA only, whereas in the nuclear data the two included sections form successive outgroups to the rest of the genus; (ii) among all 42 sections and five “series”, 25 sections and one “series” are resolved as monophyletic in both data sets; (iii) the common ancestor of Corydalis is likely to be a perennial plant with a taproot, yellow flowers with a short saccate spur, linear fruits with recurved fruiting pedicels, and seeds with elaiosomes; (iv) we provide a new classification of Corydalis with four subgenera (of which subg. Bipapillatae is here newly described), 39 sections, 16 of which are consistent with the previous classification, 16 sections have been recircumscribed, one section has been reinstated and six new sections are established. Characters associated with lifespan, underground structures, floral spur, fruit and elaiosomes are important for the recognition of subgenera and sections. These new phylogenetic analyses combined with ancestral character reconstructions uncovered previously unrecognized relationships, and greatly improved our understanding of the evolution of the genus.
作者：
Su, Hongxia;Geng, Hui;Cai, Linkang;Xu, Minjie;Xing, Wenpin;...
期刊：
Cancer Medicine,2023年12(12):13573-13585 ISSN：2045-7634
通讯作者：
Li, YK;Liu, BL
作者机构：
[Su, Hongxia; Li, Yankun] Hubei Univ Sci & Technol, Sch Pharm, Xianning, Peoples R China.;[Xu, Minjie; Xing, Wenpin; Geng, Hui] Huazhong Normal Univ, Sch Life Sci, Wuhan, Peoples R China.;[Long, Wei; Liu, Binlei; Cai, Linkang] Wuhan Binhui Biopharmaceut Co Ltd, Wuhan, Peoples R China.;[Liu, Binlei; Liu, Biao] Hubei Univ Technol, Wuhan, Peoples R China.;[Li, Yankun; Li, YK] Hubei Univ Sci & Technol, Coll Pharm, Hubei Engn Res Ctr Hubei Specialty Chinese Med, Xianning 437100, Peoples R China.
通讯机构：
[Liu, BL ; Li, YK ] H;Hubei Univ Sci & Technol, Coll Pharm, Hubei Engn Res Ctr Hubei Specialty Chinese Med, Xianning 437100, Peoples R China.;Hubei Univ Technol, Coll Bioengn, Wuhan 430068, Peoples R China.
关键词：
GM-CSF;IL-12;IL-2/15;PD-1v;tumor immunity
摘要：
The combination of immune check blockade and multiple cytokine therapy can significantly activate the body's immune response and inhibit tumor growth. Our manuscript provides a paradigm for future tumor immunotherapy. Abstract Objective In order to ensure the stable transcription of target genes, we constructed a eukaryotic high expression vector carrying an immune‐check inhibitor PD‐1v and a variety of cytokines, and studied their effects on activating immune response to inhibit tumor growth. Methods A novel eukaryotic expression plasmid vector named pT7AMPCE containing T7RNA polymerase, T7 promoter, internal ribosome entry site (IRES), and poly A tailing signal was constructed by T4 DNA ligase, on which homologous recombination was used to clone and construct the vector carrying PD‐1v, IL‐2/15, IL‐12, GM‐CSF, and GFP. In vitro transfection of CT26 cells was performed, and the protein expression of PD‐1v, IL‐12 and GM‐CSF was detected by Western blot and ELISA after 48 h. Mice were subcutaneously inoculated with CT26‐IRFP tumor cells in the rib abdomen, and the tumor tissues were injected with PD‐1v, IL‐2/15, IL‐12, and GM‐CSF recombinant plasmids for treatment during the experimental period. The efficacy of the treatment was evaluated by assay tumor size and survival time of tumor‐bearing mice during the experiment. Expression levels of IFN‐γ, TNF, IL‐4, IL‐2, and IL‐5 in mouse blood were measured using the CBA method. Tumor tissues were extracted and immune cell infiltration in tumor tissues was detected by HE staining and the IHC method. Results The recombinant plasmids carrying PD‐1v, IL‐2/15, IL‐12, and GM‐CSF were successfully constructed, and the Western blot and ELISA results showed that PD‐1v, IL‐12, and GM‐CSF were expressed in the supernatant of CT26 cells 48 h after in vitro cell transfection. The combined application of PD‐1v, IL‐2/15, IL‐12, and GM‐CSF recombinant plasmids significantly inhibited tumor growth in mice, and the tumor growth rate was significantly lower than that in the blank control group and GFP plasmid control group (p < 0.05). Cytometric bead array data suggested that the combination of PD‐1v and various cytokines can effectively activate immune cells. HE and IHC analysis revealed plenty of immune cell infiltrates in the tumor tissue, and a large proportion of tumor cells showed the necrotic phenotype in the combination treatment group. Conclusion The combination of immune check blockade and multiple cytokine therapy can significantly activate the body's immune response and inhibit tumor growth.
作者机构：
[Dai, Xiongfeng; Zhu, Manlu; Dai, XF] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.
通讯机构：
[Zhu, ML; Dai, XF ] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.
摘要：
Timely adaptation to nutrient downshift is crucial for bacteria to maintain fitness during feast and famine cycle in the natural niche. However, the molecular mechanism that ensures the timely adaption of bacterial growth to nutrient downshift remains poorly understood. Here, we quantitatively investigated the adaptation of Escherichia coli to various kinds of nutrient downshift. We found that relA deficient strain, which is devoid of stringent response, exhibits a significantly longer growth lag than wild type strain during adapting to both amino acid downshift and carbon downshift. Quantitative proteomics show that increased (p)ppGpp level promotes the growth adaption of bacteria to amino acid downshift via triggering the proteome resource re-allocation from ribosome synthesis to amino acid biosynthesis. Such type of proteome re-allocation is significantly delayed in the relA-deficient strain, which underlies its longer lag than wild type strain during amino acid downshift. During carbon downshift, a lack of stringent response in relA deficient strain leads to disruption of the transcription-translation coordination, thus compromising the transcription processivity and further the timely expression of related catabolic operons for utilizing secondary carbon sources. Our studies shed light on the fundamental strategy of bacteria to maintain fitness under nutrient-fluctuating environments. Bacteria undergo nutrient fluctuations during repeated feast and famine cycles and need to metabolically adapt to these changes. Using quantitative proteomics, Zhu & Dai show that the stringent response of (p)ppGpp is crucial for the timely adaption of bacterial growth to both amino acid and carbon downshift.
摘要：
The responsive control of energy transfer (ET) plays a key role in the broad applications of lanthanide-doped nanomaterials. Photonic crystals (PCs) are excellent materials for ET regulation. Among the numerous materials that can be used to fabricate PCs, chiral nematic liquid crystals are highly attractive due to their good photoelectric responsiveness and biocompatibility. Here, the mechanisms of ET and the photonic effect of chiral nematic structures on ET are introduced; the regulation methods of chiral nematic structures and the resulting changes in ET of lanthanide-doped nanomaterials are highlighted; and the challenges and promising opportunities for ET in chiral nematic structures are discussed.
摘要：
The large conjugated π bond in the molecular structure of carbon nanotubes (CNTs) interacts with the benzene ring structure in di (n-butyl) phthalates (DBP) through a π - π bond. Compounds of CNTs and DBP form easily, becoming another environmental pollutant of concern. We explore whether CNTs entering animals slow down the degradation of the DBP adsorbed in the CNT cavity, thereby prolonging the "hormonal activity" of DBP. In our study, male BALb/c mice were used as experimental subjects divided into four groups: the control group; the multi-walled carbon nanotubes (MWCNTs) exposure group (10mg/kg/d); the DBP exposure group (2.15 mg/kg/d); and the compound exposure group (MWCNTs + DBP). After 30 days of exposure, the mice were sacrificed and their spleens used for immunotoxicology study. The results showed that the exposure groups exhibited splenomegaly and suffered severe oxidative damage to the spleen. In the compound exposure group: levels of IgA and IgG in the serum of the mice changed, and were significantly different from levels in both the MWCNTs and DBP exposure groups (p <0.05); the pathological sections of the spleen showed that the boundary between the white pulp area (WP) and the red pulp area (RP) was blurred, that the cell arrangement was loose, and that more red blood cells were retained in the spleen. Proteomics mass spectrometry analysis showed that compared with the control group, 70 proteins were up-regulated and 27 proteins were down-regulated in the MWCNTs group, 36 proteins were up-regulated and 23 proteins were down-regulated in the DBP group, 87 proteins were up-regulated and 21 proteins were down-regulated in the compound exposure group. The results of GO enrichment analysis and KEGG enrichment analysis of the differentially expressed proteins showed that the compound exposure harmed the spleen antigen recognition, processing, and presentation, inhibited the activation and proliferation of B cells and T cells, and hindered the adaptive immune responses. Our results showed that MWCNTs and DBP compounds can damage the spleen, and impair the innate and adaptive immune functions of the body.
期刊：
JOURNAL OF PROTEOME RESEARCH,2022年21(4):1052-1060 ISSN：1535-3893
通讯作者：
Wan, CH
作者机构：
[Wan, Cuihong; Wan, CH; Zhang, Zheng; Li, Bing] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Wan, Cuihong; Wan, CH; Zhang, Zheng; Li, Bing] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Wan, CH ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
作者机构：
[Huang, Shuang-Quan; Jia, Li-Bing] Cent China Normal Univ, Inst Evolut & Ecol, Sch Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Shuang-Quan Huang] I;Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan, 430079 China
关键词：
among- and within-species variation in nectar production;Dendrobium;nectar properties;nectar secretion transition;nectar-rewarding
摘要：
Although an estimate of the deceptive pollination systems with nectarless flowers has been usually cited as one‐third of the nearly 30 000 species in Orchidaceae, the quantitative measurements of nectar production in orchid flowers remain scarce. The genus Dendrobium is species‐rich, economic and horticultural importance, a group of epiphytic orchids in tropics characteristic of nectar spur. Previous studies in southeastern Asia showed these Dendrobium species generally involving deceptive pollination, with an assumption these species are nectarless. Here we investigated nectar production and property in 34 Dendrobium species over two years. Reconstruction of phylogenetic relationship indicated that transition of nectar secretion occurred in the genus and nectar production was positively correlated with flower size. Given that 21 species produced sucrose‐dominant nectar, typical of bee‐pollinated flowers, an estimate of deceptive pollination systems awaits further survey of nectar or floral reward in diverse genera. Abstract Nectar, the most common floral reward, is generally used to determine whether an orchid species involves deceptive pollination. Estimates of the deceptive pollination systems with nectarless flowers have ranged from one quarter to one third of the nearly 30 000 species of orchids. These estimates, however, are biased towards temperate‐zone, usually terrestrial, orchids. Here we investigated nectar production and property in 34 epiphytic orchid species of the Southeast Asian genus Dendrobium. Twenty‐one species were observed producing nectar. The amount and sugar concentration (in bagged flowers) of 12 species varied from 0.45 to 2.78 μL and from 8.1% to 31.1%. The nectar was sucrose‐dominant, typical of bee‐pollinated flowers. Reconstruction of phylogenetic relationship indicated that transition of nectar secretion occurred in the genus. Spur length was positively correlated with flower size but species with relatively long spurs tended to produce small volume of nectar. Nectar production was strikingly variable among and within individuals in some species, suggesting that a vital measurement of bagged and fresh flowers is needed. Given that the quantitative measurement of nectar or floral reward in orchid species remains scarce, an estimate of deceptive pollination systems awaits further survey in diverse genera.
作者机构：
[Wan, Cuihong; Chen, Shenglan; Yang, Lin; Xu, Chen; Wang, Bing; Wang, Yaxuan; Yi, Lanxing] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.;[Wan, Cuihong; Chen, Shenglan; Yang, Lin; Xu, Chen; Wang, Bing; Wang, Yaxuan; Yi, Lanxing] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Hu, Lucas Zhongming; Emili, Andrew] Univ Toronto, Donnelly Ctr Cellular & Biomol Res, Toronto, ON M5S 2E8, Canada.;[Emili, Andrew] Boston Univ, Dept Biochem, Boston, MA 02215 USA.;[Emili, Andrew] Boston Univ, Dept Biol, 5 Cummington St, Boston, MA 02215 USA.
通讯机构：
[Wan, Cuihong] S;School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan 430079, China. Electronic address:
期刊：
International Journal of Molecular Sciences,2022年23(17):9887- ISSN：1422-0067
通讯作者：
Gengqing Huang
作者机构：
[Wang, Xinting; Wang, Xiaoqian; Huang, Gengqing; Tao, Miao; Huang, Xiaoyu] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Abuduwaili, Nigara; Huang, Gengqing] Xinjiang Normal Univ, Coll Life Sci, Xinjiang Key Lab Special Species Conservat & Regu, Urumqi 830054, Peoples R China.
通讯机构：
[Gengqing Huang] X;Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumuqi 830054, China<&wdkj&>Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China<&wdkj&>Author to whom correspondence should be addressed.
摘要：
N6-methyladenosine (m(6)A) is one of the most abundant internal modifications of mRNA, which plays important roles in gene expression regulation, and plant growth and development. Vir-like m(6)A methyltransferase associated (VIRMA) serves as a scaffold for bridging the catalytic core components of the m(6)A methyltransferase complex. The role of VIRMA in regulating leaf development and its related mechanisms have not been reported. Here, we identified and characterized two upland cotton (Gossypium hirsutum) VIRMA genes, named as GhVIR-A and GhVIR-D, which share 98.5% identity with each other. GhVIR-A and GhVIR-D were ubiquitously expressed in different tissues and relatively higher expressed in leaves and main stem apexes (MSA). Knocking down the expression of GhVIR genes by the virus-induced gene silencing (VIGS) system influences leaf cell size, cell shape, and total cell numbers, thereby determining cotton leaf morphogenesis. The dot-blot assay and colorimetric experiment showed the ratio of m(6)A to A in mRNA is lower in leaves of GhVIR-VIGS plants compared with control plants. Messenger RNA (mRNA) high-throughput sequencing (RNA-seq) and a qRT-PCR experiment showed that GhVIRs regulate leaf development through influencing expression of some transcription factor genes, tubulin genes, and chloroplast genes including photosystem, carbon fixation, and ribosome assembly. Chloroplast structure, chlorophyll content, and photosynthetic efficiency were changed and unsuitable for leaf growth and development in GhVIR-VIGS plants compared with control plants. Taken together, our results demonstrate GhVIRs function in cotton leaf development by chloroplast dependent and independent pathways.
期刊：
Journal of Proteomics,2022年266:104681 ISSN：1874-3919
通讯作者：
Cuihong Wan
作者机构：
[Wan, Cuihong; Li, Sige; Yao, Sheng; Zhan, Yuyue] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Wan, Cuihong] 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Cuihong Wan] S;School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, Hubei 430079, People's Republic of China
摘要：
Sulfolobus islandicus is thermophilic archaea that live in an extreme environment of 75°C-80°C and pH2-3. Currently, the molecular mechanism of archaeal adaptation to high temperatures and the stability of proteins at high temperatures are still unclear. This study utilizes proteomics to analyze the differential expression of S. islandicus proteins at different temperatures. We found that ribosomes, glycolysis, nucleotide metabolism, RNA metabolism, transport system, and sulfur metabolism are all affected by temperature. Methylation modification of some proteins changed with temperature. Thermal proteome profiling (TPP) was used to analyze the thermal stability of proteins under 65°C-85°C growth conditions. It is suggested that the T(m) values of proteins are mainly distributed around the optimum growth temperature (OGT). The proteins in the glycolysis pathway had high thermal stability. Meanwhile, proteins related to DNA replication and translation showed low thermal stability. The protein thermal stability of S. islandicus cultured under 65°C and 85°C was higher than that of 75°C. Our study reveals that S. islandicus may adapt to temperature changes by regulating protein synthesis and carbon metabolism pathways, changing post-translational modifications, and improving protein stability at the same time. SIGNIFICANCE: The molecular mechanism of archaeal adaptation to high temperatures and the stability of proteins at high temperatures are still unclear. Our proteomics study identified 477 differentially expressed proteins of S. islandicus at different temperatures, suggesting that ribosomes, glycolysis, nucleotide metabolism, RNA metabolism, transport system, and sulfur metabolism are affected by temperature. Meanwhile, we found that methylation modification of some proteins changed with temperature. To evaluate the thermal stability of the proteome, we performed thermal proteome profiling to analyze the Tm of proteins under 65°C-85°C growth conditions. T(m) values of proteins are mainly distributed around the optimum growth temperature. The proteins in the glycolysis pathway had high thermal stability. Meanwhile, proteins related to DNA replication and translation showed low thermal stability. Our study reveals that S. islandicus may adapt to temperature changes by regulating protein synthesis and carbon metabolism pathways, changing post-translational modifications, and improving protein stability at the same time.
