作者机构：
HUAZHONG NORMAL UNIV,DEPT BIOL,WUHAN 430070,PEOPLES R CHINA.;UNIV TSUKUBA,DEPT APPL BIOCHEM,TSUKUBA,IBARAKI 305,JAPAN.;[Tomura, D; Shoun, H] Department of Applied Biochemistry, University of Tsukuba, Ibaraki 305, Japan;[Liu, DL] Department of Biology, Huazhong Normal University, Wuhan, 430070
关键词：
cDNA cloning;Cytochrome P-460nor;Expression
摘要：
Cytochrome P-450nor is involved in the fungal denitrification and acts as a nitric oxide reductase. Tha cDNA library from Cylindrobarpon tonkinense was constructed with λgtll, and screened with antibodies. From the positive clones, the P-400nor2 cDNA fragments were recovered, and subcloned into the expression vector pYES2, then expressed in the yeast system. Western-blot analysis showed that the expressed protein, was hybridized with the antibody. Enzyme assay indicated that the expressed protein had the activities of P-450nor2, which reduced NO to form N2O, employing NADH or NADPH as the sole electron donor.
期刊：
JOURNAL OF EXPERIMENTAL BIOLOGY,1995年198(9):2007-2023 ISSN：0022-0949
通讯作者：
Philip H.-S. Jen
作者机构：
UNIV MISSOURI,DIV BIOL SCI,COLUMBIA,MO 65211.;CENT CHINA NORMAL UNIV,DEPT BIOL,WUHAN,PEOPLES R CHINA.;[CHEN, QC] Division of Biological Sciences , University of Missouri-Columbia , MO 65211, USA<&wdkj&>Department of Biology , Central China Normal University , Wuhan, Hubei, People’s Republic of China;[CAIN, D; JEN, PHS] Division of Biological Sciences , University of Missouri-Columbia , MO 65211, USA
通讯机构：
[Philip H.-S. Jen] D;Division of Biological Sciences , University of Missouri-Columbia , MO 65211, USA
摘要：
Sound pressure transformation properties at the pinna of laboratory mice Mus domesticus were studied by measuring the sound pressure level of a continuous tone at a series of frequencies at the tympanic membrane as a function of the position of a sound source under free-field stimulation conditions. The spectral transformation, the interaural spectral difference, the isopressure contours and the interaural pressure difference contours were plotted. Sound pressure transformation functions showed some prominent spectral notches throughout the frequency range tested (10-80 kHz). However, the notch frequency did not appear to be systematically related to sound direction. The study of interaural pressure difference demonstrated that, when delivered from some angles within the ipsilateral frontal hemisphere, the sound pressure at the tympanic membrane of certain frequencies may be lower than that determined at the corresponding contralateral angles. For each sound frequency tested, there was an angle (the acoustic axis) within the ipsilateral frontal hemisphere from which the delivered sound reached a maximal pressure level at the tympanic membrane. However, the acoustic axis often changed to a new angle after removal of the ipsilateral pinna. In addition, sound delivered from the acoustic axis did not always generate a maximal pressure transformation. The isopressure contours determined within 2-5 dB of the maximal pressure were circumscribed, and their contained angular areas were found to decrease with increasing sound frequency. The 2 dB maximal pressure area may appear at more than one angular area for some test frequencies. Removal of the ipsilateral pinna or modification of pinna posture expanded isopressure contours irregularly and split the 2 dB maximal pressure area into several parts. The sound pressure difference determined between the angles of maximal and minimal sound pressure (the maximal directionality) increased with sound frequency regardless of pinna posture. Acoustic gain of the pinna at the acoustic axis reached 6-12 dB, depending upon sound frequency. However, the pinna gain was not always maximal at the acoustic axis for a given frequency.
