作者机构：
Laboratoire Central de Biotechnologies, CNRA, Abidjan, Côte d’Ivoire;[Jean-Claude Veyrunes; Max Bergoin] Unité de Virologie Moléculaire, Station de Recherche de Pathologie Comparée, INRA-UA CNRS 1184, Saint-Christol-Lez-Ales, France;[Jian-xin PENG] College of Life Sciences, Huazhong Normal University, Wuhan, P.R. China;Campinas University, Sao Paulo, Brazil;[Nazaire Kouassi] Laboratoire Central de Biotechnologies, CNRA, Abidjan, Côte d’Ivoire<&wdkj&>Unité de Virologie Moléculaire, Station de Recherche de Pathologie Comparée, INRA-UA CNRS 1184, Saint-Christol-Lez-Ales, France
通讯机构：
[Yi Li] C;College of Life Sciences, Huazhong Normal University, Wuhan, P.R. China<&wdkj&>Unité de Virologie Moléculaire, Station de Recherche de Pathologie Comparée, INRA-UA CNRS 1184, Saint-Christol-Lez-Ales, France
摘要：
Pathogenicity of the Diatraea saccharalis densovirus (DsDNV) was tested on its host larvae. The results showed that up to 4 days after inoculation, no larvae mortality was observed and the infected larvae started to exhibit the infection symptoms from the fourth day. After 5 days of infection, the cumulative mortality of infected larvae increased significantly and reached 60% after 12 days and 100% after 21 days of infection, whereas that of the control group was only 10% and 20%, respectively, after same periods of infection, suggesting that the high mortality of infected larvae groups was due to the high pathogenicity of DsDNV. The size of the DsDNA was determined by Electron microscopy visualization of viral DNA molecules and gel electrophoresis of both native and endonuclease digested DNA fragments. The total length of the native DsDNA was about 5.95 kb. The DsDNV DNA was digested with 16 restriction enzymes and a restriction map of those enzymes was constructed with 41 restriction sites. Comparison of the restriction map of the DsDNV genome with those of the genomes of Junonia coenia densovirus (JcDNV) and Galleria mellonella densovirus (GmDNV) indicated that the three densovirus genomes were found to share many identical restriction sites. Thus, most of the restriction sites of the following endonucleases Bam H I, Hha I, Xba I, Cla I, Asp 700, Spe I, Nco I and Bcl I, were found to be conserved among the three densovirus genomes. Symmetrical cleavage sites mapped at the both ends of the genome suggested the presence of inverted terminal repeats (ITRs) whose size was estimated to be about 500 bp. The similar genome size, almost identical restriction sites and presence of an ITR of about 500 bp for these three densoviruses suggested that they belong to the same group of ambisense densoviruses.
作者机构：
[Peng, Jianxin] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Inst Entomol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Peng, Jianxin] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
关键词：
AfMNPV;Caspase 3;Cyclosporin A;Cytochrome c;Spodoptera litura cell line (SL-ZSU-1, SL-1)
摘要：
We investigated the influence of cytochrome c on apoptosis induced by Anagrapha (Syngrapha) falcifera multiple nuclear polyhedrosis virus (AfMNPV). Microscopic observation revealed that infection of SL-1 cells with AfMNPV resulted in apoptosis, displaying apoptotic bodies in fluorescent-stained nuclei of AfMNPV-infected SL-1cells. Western blot analysis demonstrated that AfMNPV-induced apoptosis in insect SL-1 cells was significantly inhibited by cyclosporin A which blocked a translocation of cytochrome c from the mitochondria to the cytosol. As determined by using AC-DEVD-AFC as substrate, the activity of caspase-3 in AfMNPV-induced cells was detected as early as 4 h post infection, gradually increased with time extension, and reached a highest level after 16 h of infection. However, activity of caspase-3 in apoptotic cells decreased in the presence of cyclosporin A (30 mu M), indicating that activation of caspase-3 in SfaMNPV-induced cells was dependent on the release of cytochrome c from the mitochondria. In addition, cyclosporin A could markedly inhibit mitochondrial transmembrane potential (Delta Psi m) disruption in undergoing apoptotic cells. These data indicate that cytochrome c plays a key role in AfMNPV-induced apoptosis in S. litura cells and may be required for caspase activation during the induction of apoptosis. (C) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
期刊：
Pest Management Science,2007年63(3):296-300 ISSN：1526-498X
通讯作者：
Peng, Jianxin
作者机构：
[Peng, Jianxin] Cent China Normal Univ, Inst Entomol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Peng, Jianxin] C;Cent China Normal Univ, Inst Entomol, Wuhan 430079, Peoples R China.
关键词：
Spodoptera exigua;Syngrapha falcifera multiple nucleopolyhedrovirus;peritrophic membrane;environmental scanning electron microscope;Calcofluor white M2R
作者机构：
[Liu, Kaiyu] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Liu, Kaiyu] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
作者机构：
[Liu, Kaiyu; Peng, Jianxin; Peng, Rong; Hong, Huazhu; Hong, HZ; Yang, Hong; Zheng, Jin] Cent China Normal Univ, Inst Entomol, Wuhan 430079, Peoples R China.
通讯机构：
[Hong, Huazhu] C;Cent China Normal Univ, Inst Entomol, Wuhan 430079, Peoples R China.
关键词：
Bacillus thuringiensis;Chemical pesticide;Esterase;Osmotic lysis;Resistance;Trichoplusia ni cell line
摘要：
Cabbage looper moth (Trichoplusia ni) cell line BTI-Tn-5B1-4 (TnH5) has developed high-level resistance (>1000 fold) by the selection of Bt Cry1Ac10 toxin. In order to examine mechanisms of resistance to Cry1Ac10 toxin (biological pesticide), both general esterase activities and cell tolerance to osmotic lysis were compared between non-selected Cry1Ac10-susceptible Trichoplusia ni cell line TnH5-S and Cry1Ac10-resistant Trichoplusia ni cell line TnH5-R selected by Bt Cry1Ac10. The Cry1Ac10-resistant TnH5-R cells had lower general esterase activity than the non-selected TnH5-S cells, and the esterase isozyme bands for the Cry1Ac10-resistant TnH5-R cells were much weaker than that for the non-selected TnH5-S cells. Both activated Cry1Ac10 toxin and multi-toxin from Bacillus thuringiensis subsp. aizawai GC-91 (an engineering bacterium) could not inhibit the esterase activity both in the Cry1Ac10-susceptible and Cry1Ac10-resistant cells, but two chemical pesticides, chlopyrifos and methomyl, could greatly inhibit the esterase activities both in the TnH5-R and TnH5-S cells. On the other hand, cell tolerance to osmotic lysis caused by hypotonic solution for the Cry1Ac10-resistant TnH5-R cells was higher than that for the non-selected TnH5-S cells (2.5×). Based on these results, we made the following conclusions. The general esterase activities in the Cry1Ac10-resistant TnH5-R cells was not related to Bt Cry1Ac10 resistance, but the susceptibility to the two tested chemical pesticides increased in TnH5-R cells because of their lower esterase activity. The increase of cell tolerance to osmotic lysis for the Cry1Ac10-resistant TnH5-R cells may be one of the mechanisms for Bt toxin resistance because midgut cells of insects are also disrupted by an osmotic lysis caused by Bt toxin.
作者：
Deng Shoulong;Yu Kun;Jiang Wuqi;Li Yan;Deng Zhuo;...
期刊：
微生物学报,2004年44(2):177-181 ISSN：1005-7021
作者机构：
[Deng Shoulong; Liu Yixun] Institute of Zoology, Chinese Academy of Sciences;[Yu Kun; Jiang Wuqi; Li Yan; Liu Guoshi; Lian Zhengxing] Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University;[Deng Zhuo] Department of Animal Science, Oklahoma
