饲料中添加维生素A、C和E对克氏原螯虾生长和免疫力的影响
作者：
邵光明;谭红月;王玉凤
期刊：
水产养殖 ,2017年38(4):46-52 ISSN：1004-2091
作者机构：
华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,湖北武汉430079;[谭红月; 王玉凤; 邵光明] 华中师范大学
关键词：
克氏原螯虾;维生素;存活;生长;免疫
摘要：
维生素在甲壳动物的存活、生长、繁殖过程中起重要作用。本研究采用正交设计实验,研究了维生素A、维生素C和维生素E的添加量对克氏原螯虾（Procambarus clarkii）幼虾生存、生长和免疫的影响。研究设立三个因子A、B、C,分别为维生素A、维生素C、维生素E的添加量。每个因子设3个水平,维生素A添加量的3个水平为：0.0048、0.0054、0.006%;维生素C添加量的3个水平为：0.02、0.05、0.08%;维生素E添加量的3个水平为：0.02、0.04、0.06%。极差分析结果表明,克氏原螯虾幼虾成活率最高时的因子水平组合为：A3B1/B3C2;增重率最大时的因子水平组合为：A3B2C2;丙二醛（MDA）含量、超氧化物气化酶（SOD）活性和SOD基因表达水平最低时的因子组合水平都为：A2B1C1;酚氧化酶（PO）活性最高时的因子水平组合为：A3B2C1;谷胱甘肽过氧化物酶基因GPx表达水平最低时的因子水平组合为：A3B1C1。将克氏原螯虾幼虾的成活率和增重率作为主要考虑因素,综合考虑免疫指标及成本因素,我们建议在本次实验设计的条件中,选择A3B1C2作为最佳组合方案,即维生素A添加量为0.006%、维生素C添加量为0.02%、维生素E添加量为0.04%时,最有利于克氏原螯虾幼虾的存活、生长,提高其免疫力。
语种：
中文
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C-type lectin interacting with β-integrin enhances hemocytic encapsulation in the cotton bollworm, Helicoverpa armigera
作者：
Wang, Pan;Zhuo, Xiao-Rong;Tang, Lin;Liu, Xu-Sheng
（ ;Wang, Yu-Feng;...
期刊：
Insect Biochemistry and Molecular Biology ,2017年86:29-40 ISSN：0965-1748
通讯作者：
Wang, Jia-Lin
作者机构：
[Liu, Xu-Sheng; Zhuo, Xiao-Rong; Tang, Lin; Wang, Guo-Xiu; Wang, Pan; Yu, Xiao-Qiang; Wang, Jia-Lin; Wang, Yu-Feng] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Yu, Xiao-Qiang] Univ Missouri, Sch Biol Sci, Kansas City, MO 64110 USA.
通讯机构：
[Wang, Jia-Lin] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
*20-HydroxyEcdysone;*C-type lectin;*Encapsulation;*Melanization;*beta-Integrin
摘要：
The encapsulation reaction in invertebrates is analogous to granuloma formation in vertebrates, and this reaction is severely compromised when ecdysone signaling is blocked. However, the molecular mechanism underlying the encapsulation reaction and its regulation by ecdysone remains obscure. In our previous study, we found that the C-type lectin HaCTL3, from the cotton bollworm Helicoverpa armigera, is involved in anti-bacterial immune response, acting as a pattern recognition receptor (PRR). In the current study, we demonstrate that HaCTL3 is involved in defense against parasites and directly binds to the surface of nematodes. Our in vitro and in vivo studies indicate that HaCTL3 enhances hemocytic encapsulation and melanization, whereas H. armigera β-integrin (Haβ-integrin), located on the surface of hemocytes, participates in encapsulation. Additionally, co-immunoprecipitation experiments reveal HaCTL3 interacts with Haβ-integrin, and knockdown of Haβ-integrin leads to reduced encapsulation of HaCTL3-coated beads. These results indicate that Haβ-integrin serves as a hemocytic receptor of HaCTL3 during the encapsulation reaction. Furthermore, we demonstrate that 20-hydroxyecdysone (20E) treatment dramatically induces the expression of HaCTL3, and knockdown of the 20E receptor (EcR)/ultraspiracle (USP), abrogates this response. Overall, this study provides the first evidence of the presence of a hemocytic receptor (Haβ-integrin), that interacts with the PRR HaCTL3 to facilitate encapsulation reaction in insects and demonstrates the regulation of this process by the steroid hormone ecdysone. © 2017 Elsevier Ltd
语种：
英文
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Wolbachia在中国不同地理种群果蝇中的感染检测及对dHira基因表达的影响
作者：
郑雅;王玉凤;曾庆韬;刘艳玲
作者机构：
[郑雅; 王玉凤; 曾庆韬; 刘艳玲] 华中师范大学生命科学学院;[郑雅; 王玉凤; 曾庆韬; 刘艳玲] 湖北大学生命科学学院
会议名称：
第五届广东、湖南、江西、湖北四省动物学学术研讨会
会议时间：
2008
会议地点：
中国广东广州
会议论文集名称：
第五届广东、湖南、江西、湖北四省动物学学术研讨会论文摘要汇编
关键词：
黑腹果蝇;dHira基因
摘要：
<正>Wolbachia是广泛存在于节肢动物体内的一类共生微生物,能够通过多种机制调节宿主的生殖方式,如细胞质不相容(cytoplasmic incompatibility,CI)、孤雌生殖、雌性化、杀雄等,通过这些机制使其在宿主种群内广泛传播。国内对Wolbachia在螨类、烟粉虱、蜘蛛及实蝇等体内
语种：
中文
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Knockdown of Dynamitin in testes significantly decreased male fertility in Drosophila melanogaster
作者：
Wu, Chun-Hong;Zong, Qiong;Du, An-Li;Zhang, Wei;Yao, Han-Chao;...
期刊：
Developmental Biology ,2016年420(1):79-89 ISSN：0012-1606
通讯作者：
Wang, Yu-Feng
作者机构：
[Du, An-Li; Yao, Han-Chao; Zhang, Wei; Yu, Xiao-Qiang; Zong, Qiong; Wu, Chun-Hong; Wang, Yu-Feng] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Peoples R China.;[Yu, Xiao-Qiang] Univ Missouri, Sch Biol Sci, Kansas City, MO 64110 USA.
通讯机构：
[Wang, Yu-Feng] C;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Sch Life Sci, Wuhan 430079, Peoples R China.
关键词：
Dynamitin;Drosophila melanogaster;Spermiogenesis;Mitochondria;Individualization complex;Male fertility
摘要：
Dynamitin (Dmn) is a major component of dynactin, a multiprotein complex playing important roles in a variety of intracellular motile events. We previously found that Wolbachia bacterial infection resulted in a reduction of Dmn protein. As Wolbachia may modify sperm in male hosts, we speculate that Dmn may have a function in male fertility. Here we used nosGal4 to drive Dmn knock down in testes of Drosophila melanogaster to investigate the functions of Dmn in spermatogenesis. We found that knockdown of Dmn in testes dramatically decreased male fertility, overexpression of Dmn in Wolbachia-infected males significantly rescued male fertility, indicating an important role of Dmn in inducing male fertility defects following Wolbachia infection. Some scattered immature sperm with late canoe-shaped head distributed in the end of Dmn knockdown testis and only about half mature sperm were observed in the Dmn knockdown testis relative to those in the control. Transmission electron microscopy (TEM) exhibited fused spermatids in cysts and abnormal mitochondrial derivatives. Immunofluorescence staining showed significantly less abundance of tubulin around the nucleus of spermatid and scattered F-actin cones to different extents in the individualization complex (IC) during spermiogenesis in Dmn knockdown testes, which may disrupt the nuclear condensation and sperm individualization. Since dynein-dynactin complex has been shown to mediate transport of many cellular components, including mRNAs and organelles, these results suggest that Dmn may play an important role in Drosophila spermiogenesis by affecting transport of many important cytoplasmic materials.
语种：
英文
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生物学虚拟仿真实验教学资源建设
作者：
李兵;王玉凤;贺占魁;姚汉超;熊国梅;...
期刊：
实验技术与管理 ,2016年33(12):171-173,176 ISSN：1002-4956
作者机构：
华中师范大学 生命科学学院,湖北 武汉,430079;华中师范大学 实验室与设备管理处,湖北 武汉,430079;[杨继红; 贺占魁; 姚汉超; 熊国梅; 王玉凤; 李兵; 胡原] 华中师范大学
关键词：
生物学;虚拟仿真;资源建设
摘要：
结合华中师范大学生物学国家级虚拟仿真实验教学中心建设实际,阐述了该中心基于创新人才培养的虚拟仿真实验教学资源建设的重要意义、思路、内容、成效及特色,探讨了虚拟资源建设与真实实验教学有机结合,大大提高人才培养质量的做法和体会,为高校虚拟仿真中心建设、实验教学改革和创新人才培养提供借鉴。
语种：
中文
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Sex pheromone recognition and characterization of three pheromone-binding proteins in the legume pod borer, Maruca vitrata Fabricius (Lepidoptera: Crambidae)
作者：
Mao, Aping;Zhou, Jing;Mao, Bin;Zheng, Ya;Wang, Yufeng;...
期刊：
Scientific Reports ,2016年6(1):34484 ISSN：2045-2322
通讯作者：
Ai, Hui
作者机构：
[Wang, Yufeng; Mao, Bin; Zhou, Jing; Liu, Kaiyu; Mao, Aping; Ai, Hui; Zheng, Ya] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Li, Daiqin] Natl Univ Singapore, Dept Biol Sci, 14 Sci Dr 4, Singapore 117543, Singapore.;[Wang, Pan; Wang, Xiaoping] Huazhong Agr Univ, Coll Plant Sci & Technol, Key Lab Insect Resource Utilizat & Sustainable Pr, Wuhan 430070, Peoples R China.
通讯机构：
[Ai, Hui] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
摘要：
Pheromone-binding proteins (PBPs) are essential for the filtering, binding and transporting of sex pheromones across sensillum lymph to membrane-associated pheromone receptors of moths. In this study, three novel PBP genes were expressed in Escherichia coli to examine their involvement in the sex pheromone perception of Maruca vitrata. Fluorescence binding experiments indicated that MvitPBP1-3 had strong binding affinities with four sex pheromones. Moreover, molecular docking results demonstrated that six amino acid residues of three MvitPBPs were involved in the binding of the sex pheromones. These results suggested that MvitPBP1-3 might play critical roles in the perception of female sex pheromones. Additionally, the binding capacity of MvitPBP3 with the host-plant floral volatiles was high and was similar to that of MvitGOBP2. Furthermore, sequence alignment and docking analysis showed that both MvitGOBP2 and MvitPBP3 possessed an identical key binding site (arginine, R130/R140) and a similar protein pocket structure around the binding cavity. Therefore, we hypothesized that MvitPBP3 and MvitGOBP2 might have synergistic roles in binding different volatile ligands. In combination, the use of synthetic sex pheromones and floral volatiles from host-plant may be used in the exploration for more efficient monitoring and integrated management strategies for the legume pod borer in the field.
语种：
英文
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Knockdown of ATPsyn-b caused larval growth defect and male infertility in Drosophila
作者：
Chen, Ya-Na;Wu, Chun-Hong;Zheng, Ya;Li, Jing-Jing;Wang, Jia-Lin;...
期刊：
Archives of Insect Biochemistry and Physiology ,2015年88(2):144-154 ISSN：0739-4462
通讯作者：
Wang, Yu-Feng
作者机构：
[Chen, Ya-Na; Wang, Jia-Lin; Li, Jing-Jing; Wu, Chun-Hong; Zheng, Ya; Wang, Yu-Feng] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Wang, Yu-Feng] Cent China Normal Univ, Sch Life Sci, 152 Luoyu Ave, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Wang, Yu-Feng] C;Cent China Normal Univ, Sch Life Sci, 152 Luoyu Ave, Wuhan 430079, Hubei, Peoples R China.
关键词：
ATPsyn-b;Drosophila melanogaster;larval growth;male fertility;spermatogenesis
摘要：
The ATPsyn-b encoding for subunit b of ATP synthase in Drosophila melanogaster is proposed to act in ATP synthesis and phagocytosis, and has been identified as one of the sperm proteins in both Drosophila and mammals. At present, its details of functions in animal growth and spermatogenesis have not been reported. In this study, we knocked down ATPsyn-b using Drosophila lines expressing inducible hairpin RNAi constructs and Gal4 drivers. Ubiquitous knockdown of ATPsyn-b resulted in growth defects in larval stage as the larvae did not grow bigger than the size of normal second-instar larvae. Knockdown in testes did not interrupt the developmental excursion to viable adult flies, however, these male adults were sterile. Analyses of testes revealed disrupted nuclear bundles during spermatogenesis and abnormal shaping in spermatid elongation. There were no mature sperm in the seminal vesicle of ATPsyn-b knockdown male testes. These findings suggest us that ATPsyn-b acts in growth and male fertility of Drosophila.
语种：
英文
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Drosophila Dicer-2 has an RNA interference-independent function that modulates Toll immune signaling
作者：
Wang, Zhaowei;Wu, Di;Liu, Yongxiang;Xia, Xiaoling;Gong, Wanyun;...
期刊：
Science Advances ,2015年1(9):e1500228 ISSN：2375-2548
通讯作者：
Zhou, Xi
作者机构：
[Liu, Yongxiang; Gong, Wanyun; Wang, Zhaowei; Wu, Di; Qiu, Yang; Zhou, Xi; Yang, Jie; Hu, Yuanyang; Xia, Xiaoling] Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Hubei, Peoples R China.;[Li, Jingjing; Wang, Yu-Feng; Zheng, Ya] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Xiang, Ye] Tsinghua Univ, Sch Med, Beijing 100084, Peoples R China.
通讯机构：
[Zhou, Xi] W;Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Hubei, Peoples R China.
关键词：
Dicer-2;RNA silencing;Toll signaling;invertebrate immunity;posttranscriptional gene regulation
摘要：
Dicer-2 is the central player for small interfering RNA biogenesis in the Drosophila RNA interference (RNAi) pathway. Intriguingly, we found that Dicer-2 has an unconventional RNAi-independent function that positively modulates Toll immune signaling, which defends against Gram-positive bacteria, fungi, and some viruses, in both cells and adult flies. The loss of Dicer-2 expression makes fruit flies more susceptible to fungal infection. We further revealed that Dicer-2 posttranscriptionally modulates Toll signaling because Dicer-2 is required for the proper expression of Toll protein but not for Toll protein stability or Toll mRNA transcription. Moreover, Dicer-2 directly binds to the 3' untranslated region (3'UTR) of Toll mRNA via its PAZ (Piwi/Argonaute/Zwille) domain and is required for protein translation mediated by Toll 3'UTR. The loss of Toll 3'UTR binding activity makes Dicer-2 incapable of promoting Toll signaling. These data indicate that the interaction between Dicer-2 and Toll mRNA plays a pivotal role in Toll immune signaling. In addition, we found that Dicer-2 is also required for the Toll signaling induced by two different RNA viruses in Drosophila cells. Consequently, our findings uncover a novel RNAi-independent function of Dicer-2 in the posttranscriptional regulation of Toll protein expression and signaling, indicate an unexpected intersection of the RNAi pathway and the Toll pathway, and provide new insights into Toll immune signaling, Drosophila Dicer-2, and probably Dicer and Dicer-related proteins in other organisms. © 2015 The Authors.
语种：
英文
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Quantitative Proteomic Analyses of Molecular Mechanisms Associated with Cytoplasmic Incompatibility in Drosophila melanogaster Induced by Wolbachia
作者：
Yuan, Lin-Ling;Chen, Xiulan;Zong, Qiong;Zhao, Ting;Wang, Jia-Lin;...
期刊：
JOURNAL OF PROTEOME RESEARCH ,2015年14(9):3835-3847 ISSN：1535-3893
通讯作者：
Yang, Fuquan
作者机构：
[Yuan, Lin-Ling; Wang, Jia-Lin; Zhao, Ting; Zong, Qiong; Zheng, Ya; Zhang, Ming; Wang, Yu-Feng] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Yang, Fuquan; Chen, Xiulan] Chinese Acad Sci, Inst Biophys, Key Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China.;[Yang, Fuquan; Chen, Xiulan] Chinese Acad Sci, Inst Biophys, Lab Prote, Beijing 100101, Peoples R China.;[Wang, Zailong] Novartis Pharmaceut, E Hanover, NJ 07936 USA.;[Brownlie, Jeremy C.] Griffith Univ, Sch Nat Sci, Nathan, Qld 4111, Australia.
通讯机构：
[Yang, Fuquan] C;Chinese Acad Sci, Inst Biophys, Key Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China.
关键词：
Drosophila melanogaster;Wolbachia;iTRAQ;quantitative proteomics;spermatheca;seminal receptacle
摘要：
To investigate the molecular mechanisms of cytoplasmic incompatibility (CI) induced by Wolbachia bacteria in Drosophila melanogaster, we applied an isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic assay to identify differentially expressed proteins extracted from spermathecae and seminal receptacles (SSR) of uninfected females mated with either 1-day-old Wolbachia-uninfected (1T) or infected males (1W) or 5-day-old infected males (5W). In total, 1317 proteins were quantified; 83 proteins were identified as having at least a 1.5-fold change in expression when 1W was compared with 1T. Differentially expressed proteins were related to metabolism, immunity, and reproduction. Wolbachia changed the expression of seminal fluid proteins (Sfps). Wolbachia may disrupt the abundance of proteins in SSR by affecting ubiquitin-proteasome-mediated proteolysis. Knocking down two Sfp genes (CG9334 and CG2668) in Wolbachia-free males resulted in significantly lower embryonic hatch rates with a phenotype of chromatin bridges. Wolbachia-infected females may rescue the hatch rates. This suggests that the changed expression of some Sfps may be one of the mechanisms of CI induced by Wolbachia. This study provides a panel of candidate proteins that may be involved in the interaction between Wolbachia and their insect hosts and, through future functional studies, may help to elucidate the underlying mechanisms of Wolbachia-induced CI. © 2015 American Chemical Society.
语种：
英文
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Identification of Wolbachia-mediated Small Non-coding RNA in Testis from Drosophila melanogaster
作者：
Ya Zheng;Rui-Fang Wang;Jie Bi;Yu-Feng Wang
作者机构：
[Ya Zheng; Rui-Fang Wang; Jie Bi; Yu-Feng Wang] Hubei Key Laboratory of Genetic Regulation and Integrative Biology,College of Life Science,Central China Normal University
会议名称：
The 5th International Symposium on Insect Physiology, Biochemistry and Molecular Biology
会议时间：
2015-01-01
会议地点：
中国广东广州
摘要：
Wolbachia are endosymbiotic bacteria that are frequently found in arthropods and nematodes. These maternally inherited bacteria manipulate host reproduction by several mechanisms including cytoplasmic
语种：
英文
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黑腹果蝇Mst84Db敲降引起的父本缺陷类似Wolbachia诱导的细胞质不亲和
作者：
王翠平;郑雅;郑金晶;王玉凤
期刊：
昆虫学报 ,2014年57(3):293-299 ISSN：0454-6296
作者机构：
华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,武汉430079;[王翠平; 郑金晶; 王玉凤; 郑雅] 华中师范大学
关键词：
黑腹果蝇;基因敲降;细胞质不亲和
摘要：
【目的】Wolbachia 是广泛存在于昆虫体内的一类通过母系传递的共生菌,能够通过多种方式影响宿主的生殖。细胞质不亲和(CI)是Wolbachia 引起的最普遍的一种表型,即感染Wolbachia 的雄性和未感染的雌性宿主交配后,胚胎发育停滞于早期阶段。但目前有关CI 的分子机理还不清楚。本研究组前期实验表明,Wolbachia 感染引起黑腹果蝇Drosophila melanogaster 3龄幼虫精巢中Mst84Db 基因的表达显著下调。本研究的目的是进一步研究Mst84Db 与CI 的关系。【方法】我们体外合成了Mst84Db 的双链RNA(dsRNA),注射雄性果蝇,将注射过的雄果蝇与野生雌果蝇交配,检测其繁殖力。基因表达采用定量RT-PCR 方法进行检测。胚胎表型采用DAPI 染色进行分析。【结果】注射dsRNA 24h 后,Mst84Db 基因的表达水平发生显著下调。注射后72h,基因表达下调幅度最大。与对照组相比,基因敲降后的雄蝇繁殖能力显著下降,与雌果蝇交配后胚胎孵化率显著低于对照组,这与Wolbachia诱导的CI 现象类似。未孵化胚胎的表皮上没有体节出现,说明胚胎停滞于发育的早期。部分胚胎细胞核分裂不同步,且有染色质间桥出现,这也与CI 胚胎中的细胞学表型一致。【结论】Wolbachia 感染可能抑制果蝇精子发生过程中Mst84Db 基因的表达,从而使精子失去正常功能,最终导致与雌性果蝇交配后,胚胎发育停滞,并最终死亡。Mst84Db 基因在雄性果蝇中表达下调可能是产生CI 的重要原因之一。
语种：
中文
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Wolbachia-induced paternal defect in Drosophila is likely by interaction with the juvenile hormone pathway
作者：
Liu, Chen;Wang, Jia-Lin;Zheng, Ya;Xiong, En-Juan;Li, Jing-Jing;...
期刊：
Insect Biochemistry and Molecular Biology ,2014年49(1):49-58 ISSN：0965-1748
通讯作者：
Wang, Yu-Feng
作者机构：
[Yuan, Lin-Ling; Yu, Xiao-Qiang; Liu, Chen; Wang, Jia-Lin; Xiong, En-Juan; Li, Jing-Jing; Zheng, Ya; Wang, Yu-Feng] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Yu, Xiao-Qiang] Univ Missouri, Sch Biol Sci, Kansas City, MO 64110 USA.
通讯机构：
[Wang, Yu-Feng] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
CG10433;Drosophila melanogaster;JhI-26;Juvenile hormone;Paternal defects;Wolbachia
摘要：
Wolbachia are endosymbionts that infect many insect species. They can manipulate the host's reproduction to increase their own maternal transmission. Cytoplasmic incompatibility (CI) is one such manipulation, which is expressed as embryonic lethality when Wolbachia-infected males mate with uninfected females. However, matings between males and females carrying the same Wolbachia strain result in viable progeny. The molecular mechanisms of CI are currently not clear. We have previously reported that the gene Juvenile hormone-inducible protein 26 ( JhI-26) exhibited the highest upregulation in the 3rd instar larval testes of Drosophila melanogaster when infected by Wolbachia. This is reminiscent of an interaction between Wolbachia and juvenile hormone (JH) pathway in flies. Considering that Jhamt gene encodes JH acid methyltransferase, a key regulatory enzyme of JH biosynthesis, and that methoprene-tolerant (Met) has been regarded as the best JH receptor candidate, we first compared the expression of Jhamt and Met between Wolbachia-infected and uninfected fly testes to investigate whether Wolbachia infection influence the JH signaling pathway. We found that the expressions of Jhamt and Met were significantly increased in the presence of Wolbachia, suggesting an interaction of Wolbachia with the JH signaling pathway. Then, we found that overexpression of JhI-26 in Wolbachia-free transgenic male flies caused paternal-effect lethality that mimics the defects associated with CI. JhI-26 overexpressing males resulted in significantly decrease in hatch rate. Surprisingly, Wolbachia-infected females could rescue the egg hatch. In addition, we showed that overexpression of JhI-26 caused upregulation of the male accessory gland protein (Acp) gene CG10433, but not vice versa. This result suggests that JhI-26 may function at the upstream of CG10433. Likewise, overexpression of CG10433 also resulted in paternal-effect lethality. Both JhI-26 and CG10433 overexpressing males resulted in nuclear division defects in the early embryos. Finally, we found that Wolbachia-infected males decreased the propensity of the mated females to remating, a phenotype also caused by both JhI-26 and CG10433 overexpressing males. Taken together, our results provide a working hypothesis whereby Wolbachia induce paternal defects in Drosophila probably by interaction with the JH pathway via JH response genes JhI-26 and CG10433. © 2014 Elsevier Ltd.
语种：
英文
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Involvement of a pattern recognition receptor C-type lectin 7 in enhancing cellular encapsulation and melanization due to its carboxyl-terminal CRD domain in the cotton bollworm, Helicoverpa armigera
作者：
Wang, Jia-Lin
* （ ;Zhang, Qi;Tang, Lin;Chen, Lei;Liu, Xu-Sheng;...
期刊：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY ,2014年44(1):21-29 ISSN：0145-305X
作者机构：
[Wang, Jia-Lin; Zhang, Qi; Tang, Lin; Chen, Lei; Liu, Xu-Sheng; Wang, Yu-Feng] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, Jia-Lin] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
C-type lectin;CRD;Encapsulation;Melanization;Helicoverpa armigera
摘要：
C-type lectins play important roles in innate immunity as pattern recognition receptors (PRRs). We have previously reported a novel C-type lectin HaCTL7 from the cotton bollworm (Helicoverpa armigera) which contains two carbohydrate-recognition domains (CRDs), namely N-terminal CRD1 and C-terminal CRD2. Interestingly, there are four but not six of conserved cysteine residues in CRD2 of HaCTL7, which is different from that of other dual CRD C-type lectins. In the current study, we expressed and purified recombinant HaCTL7 (rHaCTL7) as well as rCRD1 and rCRD2, and demonstrated that both rHaCTL7 and rCRD2, but not rCRD1, owned the agglutinate ability against both Gram-negative and Gram-positive bacteria in a calcium dependent manner. In addition, both rHaCTL7 and rCRD2, but not rCRD1, could bind to various bacteria, and enhanced haemocytes mediated encapsulation and melanization processes. HaCTL7 secreted from fat bodies is able to bind to granulocytes, plasmatocytes and oenocytoids, but not to spherulocytes. Recombinant HaCTL7 and rCRD2 are capable of binding to both granulocytes and oenocytoids, while rCRD1 can only bind to granulocytes. Our data suggest that as a PRR HaCTL7 enhances encapsulation and melanization likely through its C-terminal CRD2, but not N-terminal CRD1, which imply that the characteristic four cysteine structure of CRD2 plays key roles in innate immunity. © 2013 Elsevier Ltd.
语种：
英文
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SRP gene is required for Helicoverpa armigera prophenoloxidase activation and nodulation response
作者：
Qiao, Chuan;Li, Jie;Wei, Xiu-Hong;Wang, Jia-Lin;Wang, Yu-Feng;...
期刊：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY ,2014年44(1):94-99 ISSN：0145-305X
作者机构：
[Liu, Xu-Sheng; Li, Jie; Wang, Jia-Lin; Qiao, Chuan; Wei, Xiu-Hong; Wang, Yu-Feng] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Liu, Xu-Sheng] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Helicoverpa armigera;SRP;Prophenoloxidase activation;Nodulation;Innate immunity
摘要：
SRP gene was first identified from the fall webworm, Hyphantria cunea as one of genes up-regulated after bacteria injection. A rent study in Spodoptera litura showed that stress-induced elevation of SRP expression highly correlates with reduced feeding activities and growth retardation of larvae. In this study, we identified a SRP gene from the cotton bollworm, Helicoverpa armigera, namely Ha-SRP, and studied its precise roles in insect immunity. Expressions of Ha-SRP were upregulated in H. armigera larval hemocytes after injection of Escherichia coli. When the expression of Ha-SRP in H. armigera larval hemocytes was inhibited by dsHa-SRP injection, the transcription of prophenoloxidase genes in hemocytes was repressed, phenoloxidase activity in bacteria-challenged larval hemolymph was significantly decreased, and nodule formation in bacteria-injected larvae was reduced. More importantly, RNAi-treated insects infected with E. coli showed higher bacterial growth in hemolymph compared with infected controls. These results suggest that Ha-SRP gene plays importance roles in H. armigera innate immunity, possibly by mediating prophenoloxidase activation and nodulation response.
语种：
英文
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HIRA is essential for the development of gibel carp
作者：
Wang, Meng-Yu;Guo, Qiu-Hong;Du, Xin-Zheng;Zhou, Li;Luo, Qian;...
期刊：
Fish Physiology and Biochemistry ,2014年40(1):235-244 ISSN：0920-1742
通讯作者：
Wang, Yu-Feng
作者机构：
[Du, Xin-Zheng; Luo, Qian; Zhou, Li; Wang, Meng-Yu; Wang, Jia-Lin; Guo, Qiu-Hong; Zeng, Qiao-Hui; Zhao, Hao-Bin; Wang, Yu-Feng] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, Yu-Feng] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
HIRA;Gibel carp;Embryonic development;Organogenesis
摘要：
HIRA is one of the chaperones of histone H3.3. Mutation of Hira results in embryonic lethality in mice, suggesting a critical role in embryogenesis. However, Hira-mutated Drosophila may survive to adults, indicating that it is dispensable in Drosophila development. The role of Hira in fish development is unknown. In this study we first investigated the expression of Hira during embryogenesis of gibel carp (Carassius auratus gibelio) by whole-mount in situ hybridization. We found that Hira signal appeared ubiquitously in the early embryos. After gastrulation, it appeared mainly along the anterior-posterior axis, including the tail bud. In hatching period, the signal was detected in head, heart, and the endoderm region on the back of yolk. Then by microinjection with morpholino-HIRA at the beginning of development, we observed delayed gastrulation and abnormal somitogenesis in gibel carp embryos. The HIRA morphants exhibited short trunk, limited yolk extension, and twisted tail. Most of the mutants died during embryogenesis or shortly after hatching. The rest of the HIRA morphants could survive to larvae but with severe defects in organogenesis. These data suggest that HIRA may be essential for the development of gibel carp, and this function is conserved in vertebrates. © 2013 Springer Science+Business Media Dordrecht.
语种：
英文
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中学教师继续教育的需求及影响因素研究
作者：
秦雪梅;王玉凤
期刊：
教师教育论坛 ,2014年(6):69-75 ISSN：2095-5995
作者机构：
华中师范大学 生命科学学院,武汉,430079;[秦雪梅; 王玉凤] 华中师范大学
关键词：
中学教师;继续教育;需求;影响因素;教师专业发展
摘要：
为了解中学教师继续教育的现状,本文采用问卷调查、访谈等方法研究了5个省16所中学的教师继续教育过程中存在的问题及影响因素。研究发现,中学教师继续教育覆盖面较广,但教育效果不佳,主要反映在教学模式比较单一,教学内容理论性太强,没有针对性,不太实用等方面。中学教师对继续教育的需求比较一致,如期望的教学模式为交流互动式、时间安排希望在寒暑假集中培训、教学内容希望多增加一些学科前沿知识等;教师继续教育经费投入不足、课程设置不合理、工作和学习不能兼顾等因素已经严重影响到教师对继续教育的积极性。建议通过制度建设、教学模式改革、优化师资队伍等一系列措施进一步保障中学教师继续教育的科学性和实效性,最终促进教师专业化发展,推动基础教育改革的进程,提高基础教育的质量。
语种：
中文
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20-hydroxyecdysone transcriptionally regulates humoral immunity in the fat body of Helicoverpa armigera
作者：
Wang, J-L.;Chen, L.;Tang, L.;Zhao, H-B.;Liu, X-S.
* （ ;...
期刊：
Insect Molecular Biology ,2014年23(6):842-856 ISSN：0962-1075
作者机构：
[Liu, X-S.; Wang, Y-F.; Chen, L.; Tang, L.; Wang, J-L.; Zhao, H-B.] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Liu, X-S.] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Helicoverpa armigera;transcriptome;digital gene expression (DGE);fat body;immunity;20-hydroxyecdysone (20E)
摘要：
20-hydroxyecdysone (20E) increases its titre level during the wandering stage and influences innate immunity in many holometabolous insects. However, the function of 20E as an immune-activator or -suppressor needs to be determined. Here, the transcriptome of the peptidoglycan-challenged fat body of the cotton bollworm, Helicoverpa armigera, was analysed using Illumina sequencing technology. Overall, 32 073 unigenes were assembled with a mean length of 643 nucleotides. Gene expression dynamics in the fat body during the wandering stage and of peptidoglycan-challenged individuals were investigated by the digital gene expression system. Pattern recognition receptors [such as peptidoglycan recognition protein B (PGRP B), PGRP S2 precursor, C-type lectin 5, hemolin and β-1,3-glucan recognition protein 2a] and antimicrobial peptides (namely attacin, gloverin, gloverin precursor, gloverin-like, cecropin 2, cecropin D, cecropin D-like and i-type lysozyme) significantly increased their mRNA levels during the wandering stage. 20E treatment significantly induced the expression of these genes. Antibacterial activities were also enhanced during the wandering stage and after 20E injections. Bacillus subtilis peptidoglycan induced the expression of PGRP D, PGRP B, PGRP S2 precursor, gloverin, gloverin precursor, gloverin-like, cecropin 2, cecropin D and lebocin-like genes. These results demonstrate that 20E acts by enhancing humoral immunity in H. armigera. © 2014 The Royal Entomological Society.
语种：
英文
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我国三地区黑腹果蝇中Wolbachia 的系统发育关系及其对宿主生殖的影响
作者：
熊恩娟;郑雅;王玉凤;曾庆韬
期刊：
昆虫学报 ,2014年57(2):176-186 ISSN：0454-6296
作者机构：
[熊恩娟; 郑雅; 王玉凤] 华中师范大学生命科学学院;[曾庆韬] 湖北大学生命科学学院
关键词：
黑腹果蝇;wsp 基因;多位点序列分型;细胞质不亲和;系统发育
摘要：
【目的】Wolbachia 是广泛存在于节肢动物和丝状线虫体内的一类共生菌,能够以多种方式对宿主产生影响。精卵细胞质不亲和( CI)是其引起的最普遍的表型,即感染Wolbachia的雄性宿主与未感染或感染不同品系的雌性宿主交配后,不能产生后代或后代极少,而感染同品系Wolbachia的雌雄宿主交配后则能正常产生后代。我们前期研究发现,湖北武汉、云南六库和天津3个地区黑腹果蝇Drosophila melanogaster被Wolbachia感染。本研究旨在明确这3个地区黑腹果蝇中Wolbachia的系统发育关系及其对宿主生殖的影响。【方法】利用Clustal X软件对 Wolbachia的wsp基因序列进行比对,利用MEGA软件构建系统发育树。采用多位点序列分型( MLST)的方法对 Wolbachia进行分型。通过区内交配和区之间杂交的方式研究不同地区黑腹果蝇体内Wolbachia 的关系及其对果蝇生殖的影响。【结果】湖北武汉、云南六库和天津3个地区黑腹果蝇中感染的Wolbachia都是属于A大组的Mel亚群。这3个地区果蝇感染的Wolbachia的序列类型( ST)不同,Wolbachia之间存在一定的差异。湖北武汉和天津果蝇中的Wolbachia 能引起强烈的CI表型,而云南六库果蝇中的Wolbachia引起的CI强度相对较弱。武汉果蝇中 Wolbachia 不能完全挽救天津果蝇中Wolbachia引起的CI表型,而天津果蝇中Wolbachia也不能完全挽救武汉果蝇中Wolbachia 引起的CI 表型。【结论】武汉和天津地区黑腹果蝇中的Wolbachia可能距离较远。Wolbachia的长期共生可能对黑腹果蝇的进化产生了一定的影响,湖北武汉与云南六库的黑腹果蝇中感染的Wolbachia属于不同的序列类型,这2个地区的黑腹果蝇已发生了一定的分歧,产生了一定的生殖隔离。
语种：
中文
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Understanding the Mechanisms of Cytoplasmic incompatibility induced by Wolbachia in insects
作者：
Ya Zheng;Lin-Ling Yuan;Jia-Ling Wang;Chen Liu;Cui-Ping Wang;...
作者机构：
[Ya Zheng; Lin-Ling Yuan; Jia-Ling Wang; Chen Liu; Cui-Ping Wang; Yu-Feng Wang] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University Wuhan, 430079, P.R.China
会议名称：
The 10th Conference of Insect Physiology, Biochemistry and Molecular Biology(第四届国际昆虫生理生化与分子生物学学术研讨会暨第十届全国昆虫生理生化与分子生物学学术会议)
会议时间：
2013-6-15
会议地点：
南京
会议主办单位：
中国昆虫学会
会议论文集名称：
The 10th Conference of Insect Physiology, Biochemistry and Molecular Biology(第四届国际昆虫生理生化与分子生物学学术研讨会暨第十届全国昆虫生理生化与分子生物学学术会议)论文集
摘要：
<正>Wolbachia are obligate endosymbiotic bacteria that infect numerous species of arthropods and nematodes.They can induce several reproductive phenotypes in their insect hosts including cytoplasmic in
语种：
英文
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Cloning and functional analysis of glutathione peroxidase gene in red swamp crayfish Procambarus clarkii
作者：
Xia, Xiao-Fei;Zheng, Jin-Jing;Shao, Guang-Ming;Wang, Jia-Lin;Liu, Xu-Sheng
（ ;...
期刊：
Fish & Shellfish Immunology ,2013年34(6):1587-1595 ISSN：1050-4648
作者机构：
[Liu, Xu-Sheng; Shao, Guang-Ming; Zheng, Jin-Jing; Wang, Jia-Lin; Xia, Xiao-Fei; Wang, Yu-Feng] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Wang, Yu-Feng] Cent China Normal Univ, Coll Life Sci, 152 Luoyu Ave, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, Yu-Feng] C;Cent China Normal Univ, Coll Life Sci, 152 Luoyu Ave, Wuhan 430079, Peoples R China.
关键词：
Procambarus clarkii;GPx;Expression patterns;Microbe challenge;ROS
摘要：
Glutathione peroxidases (GPxs) are key enzymes in the antioxidant defense systems of living organisms, including crustaceans. The red swamp crayfish Procambarus clarkii is the most commonly farmed freshwater crayfish in Chinese inland nowadays due to its commercial value. However, high stocking density has resulted in adverse effects in growth performance and health. To investigate the function of GPxs in immune defense of the crayfish, we cloned and characterized a full length GPx (. PcGPx) from P. clarkii by a reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The 931 bp PcGPx cDNA contains a 38 bp 5'-untranslated region (UTR), a 519 bp coding sequence (CDS) and a 375 bp 3'-UTR with a selenocysteine insertion sequence (SECIS). The PcGPx was predicted to encode 172 amino acids, and its putative molecular mass was 20.9kDa with a pI of 4.37. A selenocysteine (Sec) encoded by the unusual stop codon, TGA, was in the protein coding region. Phylogenetic analysis showed that PcGPx clustered with the GPxs from the penaeid shrimp Metapenaeus ensis and Caenorhabditis elegans, sharing much higher similarity with vertebrate GPx1 and GPx2 than with GPx3 and GPx5. Quantitative RT-PCR revealed that PcGPx was extremely highly expressed in ovary and early embryos. In addition, the levels of PcGPx mRNA and reactive oxygen species (ROS) significantly increased after challenge with gram-negative Vibrio harveyi, gram-positive Staphyloccocus aureus or white spot syndrome virus (WSSV). These results suggest that PcGPx may play important roles not only in immune defense, but also in oogenesis in the crayfish. © 2013 Elsevier Ltd.
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英文
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