作者：
Du, X. Z.;Zhou, L.;Zhao, H. B.;Wang, Y. F.*;Gui, J. F.
期刊：
Fish Physiology and Biochemistry,2008年34(2):175-184 ISSN：0920-1742
通讯作者：
Wang, Y. F.
作者机构：
[Zhao, H. B.; Du, X. Z.; Wang, Y. F.; Zhou, L.] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Gui, J. F.] Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
通讯机构：
[Wang, Y. F.] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
Hir/Hira (histone regulation) genes were first identified in yeast as negative regulators of histone gene expression. It has been confirmed that HIRA is a conserved family of proteins present in various animals and plants. In this paper, the cDNAs of the Hira homolog named CagHira and CaHira were isolated from gynogenetic gibel carp (gyno-carp) and gonochoristic color crucian carp (gono-carp) respectively. The full-length CagHira is 3,860 bp in length with an open reading frame (ORF) of 3,033 bp that encodes 1,011 amino acids, while the full-length CaHira is 3,748 bp in length and also has an ORF of 3,033 bp. The deduced amino acid sequences of both Hira homologs contain seven WD domains and show high identity with other HIRA family members. RT-PCR analyses revealed strong expression of Hira in the ovaries, whereas no expression was detected in the testes of either of the fishes. Hira transcription was not detected in the liver of gyno-carp, but a high level of Hira mRNA was observed in gono-carp. The temporal expression pattern showed that the Hira mRNA is consistently expressed during all embryonic development stages in gyno-carp. However, the abundance of CaHira mRNA significantly decreased (P < 0.05) shortly after fertilization and then increased again and remained stable from gastrula till hatching. The varying spatiotemporal expression patterns of Hira genes in gyno-carp and gono-carp may be associated with the differing reproductive modes used by these two closely related fishes. Our results suggest that Hira may play a role not only in the decondensation of sperm nucleus and the formation of pronucleus during fertilization, but also in gastrulation and the subsequent development of embryos.
摘要：
实验用不同的维生素 E(VE)含量的饲料投喂克氏原螯虾亲虾,研究对其繁殖性能的影响,目的是为研制亲虾的人工饲料提供指导。结果显示,在添加 VE 量分别为基础饲料干重的 0%、0.004%、0.02%、0.1%和0.5%的5个饲料组中,0.02%VE 组不仅可以增加产卵雌虾的数量比例,而且也能提高克氏原螯虾雌虾的产卵量。随着 VE 含量的升高,产卵雌虾的比例呈下降趋势,单个雌虾的产卵量与0.02%V
摘要：
Abstract Copper is an important ingredient in fungicides, which are used for many economic plants. However, there is concern about the side‐effects of copper‐based fungicides due to their potential to affect beneficial mites. The purpose of the present paper was to investigate the effects of copper on the development of the fruit fly, Drosophila melanogaster. It was found that higher doses of copper significantly prolonged the developmental time of the fruit flies, especially during the larval stages. When 320 mg L‐1 copper‐contaminated food was used, most of the larvae died when they were small and before the pupal stage. The protein contents and esterase isozymes extracted from the larvae changed according to the doses of copper. The small‐molecular‐weight protein bands gradually became weaker or were lost as the copper levels increased. However, low doses of copper stimulated a stronger expression of a few proteins. These results indicate that low doses of copper generally have no lethal effects on D. melanogaster because a specific group of genes, which encode specific proteins, are probably activated in order to withstand the onslaught of stressful conditions. At high doses of copper in food (e.g. 320 mg L‐1), fly development and viability are significantly affected.
