作者：
Biwot, John C.;Zhang, Hua-Bao;Chen, Meng-Yan;Wang, Yu-Feng*
期刊：
Archives of Insect Biochemistry and Physiology,2019年102(4):e21612 ISSN：0739-4462
通讯作者：
Wang, Yu-Feng
作者机构：
[Zhang, Hua-Bao; Chen, Meng-Yan; Biwot, John C.; Wang, Yu-Feng] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.;[Wang, Yu-Feng] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Wang, Yu-Feng] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
摘要：
Zn72D encodes the Drosophila zinc finger protein Zn72D. It was first identified to be involved in phagocytosis and indicated to have a role in immunity. Then it was demonstrated to have a function in RNA splicing and dosage compensation in Drosophila melanogaster. In this study, we discovered a new function of Zn72D in male fertility. We showed that knockdown of Zn72D in fly testes caused an extremely low egg hatch rate. Immunofluorescence staining of Zn72D knockdown testes exhibited scattered spermatid nuclei and no actin cones or individualization complexes (ICs) during spermiogenesis, whereas the early-stage germ cells and the spermatocytes were observed clearly. There were no mature sperms in the seminal vesicles of Zn72D knockdown fly testes, although a few sperms could be found close to the seminal vesicle. We further showed that many cytoskeleton-related genes were significantly downregulated in fly testes due to Zn72D knockdown. Taken together these findings suggest that Zn72D may have an important function in spermatogenesis by sustaining the cytoskeleton-based morphogenesis and individualization thus ensuring the proper formation of sperm in D. melanogaster.
期刊：
Journal of Biological Chemistry,2019年294(26):10172-10181 ISSN：0021-9258
通讯作者：
Yu, Xiao-Qiang;Strand, Michael R.
作者机构：
[He, Zhen; Yu, Xiao-Qiang; Li, Chun-Feng; Chowdhury, Munmun] Univ Missouri, Sch Biol Sci, Div Cell Biol & Biophys, Kansas City, MO 64110 USA.;[Liu, Xu-Sheng; He, Zhen; Yu, Xiao-Qiang; Wang, Yu-Feng] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Yu, Xiao-Qiang; Lu, Yuzhen] South China Normal Univ, Guangzhou Key Lab Insect Dev Regulat & Applicat R, Inst Insect Sci & Technol, Guangzhou 510631, Guangdong, Peoples R China.;[Yu, Xiao-Qiang; Lu, Yuzhen] South China Normal Univ, Sch Life Sci, Guangzhou 510631, Guangdong, Peoples R China.;[Li, Chun-Feng] Southwest Univ, State Key Lab Silkworm Genome Biol, Chongqing 400716, Peoples R China.
通讯机构：
[Yu, Xiao-Qiang; Strand, Michael R.] U;Univ Missouri, Sch Biol Sci, Div Cell Biol & Biophys, Kansas City, MO 64110 USA.;Univ Georgia, Dept Entomol, Athens, GA 30602 USA.
作者机构：
[Wang, Yufeng; Mao, Bin; Fang, Nainai; Bi, Jie; Ai, Hui; Zheng, Ya] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Guan, Chuxiong; Hu, Yuwei; Li, Jihu; Mao, Yongkai] Guangzhou Sugarcane Ind Res Inst, Guangdong Prov Bioengn, Guangdong Key Lab Sugarcane Improvement & Biorefi, Guangzhou 510316, Guangdong, Peoples R China.
通讯机构：
[Ai, Hui] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
摘要：
Insect pheromone-binding proteins (PBPs) have been proposed to capture and transport hydrophobic sex pheromone components emitted by con-specific insects to pheromone receptors in the hemolymph of male antennal sensilla. In this study, field trapping results indicate that a mixture of E11-16: Ald and Z11-16: Ald can effectively attract a great number of male Tryporyza intacta. Real-time PCR results suggest that the transcript levels of three TintPBP1-3 genes are mainly expressed in the adult antennae. Fluorescence competitive binding experiments show that TintPBP1-3 proteins have great binding affinities to their major sex pheromones. Moreover, TintPBPs clearly cannot bind to other four kinds of sex pheromone components released by another sugarcane borer, Chilo venosatus and Chilo infuscatellu, which have the same host plant and live in similar habitats like T. intacta. The molecular docking results demonstrate that six amino acid residues of the three TintPBPs are crucial for the specific perception of the sex pheromone components. These results will provide a foundation for the development of novel sex pheromone analogues and blocking agents for biological control of sugarcane pests, improving their efficient monitoring and integrated management strategies in the sugarcane field.
摘要：
Wolbachia are endosymbiotic bacteria present in a wide range of insects. Although their dramatic effects on host reproductive biology have been well studied, the effects of Wolbachia on sleep behavior of insect hosts are not well documented. In this study, we report that Wolbachia infection caused an increase of total sleep time in both male and female Drosophila melanogaster. The increase in sleep was associated with an increase in the number of nighttime sleep bouts or episodes, but not in sleep bout duration. Correspondingly, Wolbachia infection also reduced the arousal threshold of their fly hosts. However, neither circadian rhythm nor sleep rebound following deprivation was influenced by Wolbachia infection. Transcriptional analysis of the dopamine biosynthesis pathway revealed that two essential genes, Pale and Ddc, were significantly upregulated in Wolbachia-infected flies. Together, these results indicate that Wolbachia mediates the expression of dopamine related genes, and decreases the sleep quality of their insect hosts. Our findings help better understand the host-endosymbiont interactions and in particular the Wolbachia's impact on behaviors, and thus on ecology and evolution in insect hosts.
摘要：
Diverse stimuli induce stomatal closure by triggering the efflux of osmotic anions, which is mainly mediated by the main anion channel SLAC1 in plants, and the anion permeability and selectivity of SLAC1 channels from several plant species have been reported to be variable. However, the genetic identity as well as the anion permeability and selectivity of the main S-type anion channel ZmSLAC1 in maize are still unknown. In this study, we identified GRMZM2G106921 as the gene encoding ZmSLAC1 in maize, and the maize mutants zmslac1-1 and zmslac1-2 harboring a mutator (Mu) transposon in ZmSLAC1 exhibited strong insensitive phenotypes of stomatal closure in response to diverse stimuli. We further found that ZmSLAC1 functions as a nitrate-selective anion channel without obvious permeability to chloride, sulfate and malate, clearly different from SLAC1 channels of Arabidopsis thaliana, Brassica rapa ssp. chinensis and Solanum lycopersicum L. Further experimental data show that the expression of ZmSLAC1 successfully rescued the stomatal movement phenotypes of the Arabidopsis double mutant atslac1-3atslah3-2 by mainly restoring nitrate-carried anion channel currents of guard cells. Together, these findings demonstrate that ZmSLAC1 is involved in stomatal closure mainly by mediating the efflux of nitrate in maize.
作者：
He Zhen;Zhang Huabao;Li Shitian;Yu Wenjuan;Peng Yu;...
期刊：
华中昆虫研究,2018年(00):351-351
作者机构：
School oi Life Sciences,Hubei key laboratory oi genetic regulation and integrative biology,Central China Normal University,Wuhan430079,China;School of Life Sciences,Hubei University,Wuhan430062,China
摘要：
The series of stereotypical physiological and behavioral changes that female insects exhibit after mating are called postmating responses(PMR). Wolbachia are widespread intracellular bacteria that are well known for their ability to manipulate the host’s reproductive behavior to facilitate their own maternal spreading. The effect of Wolbachia infection on insect hosts’ PMR is not well understood. Here we showed that after mating with male Drosophila melanogaster infected with Wolbachia, the uninfected female showed a significant decrease in egg laying on the first day. Furthermore,both Wolbachia-infected and uninfected females mated with infected males exhibited reduced feeding frequency,low receptivity to remating,and an extended median life span compared to those mated with uninfected males. To determine how Wolbachia triggered these alterations, we detected the influence of Wolbachia infection on the expression of some genes encoding seminal fluid proteins(Sfps) in D. melanogaster. These seminal fluid proteins are known to contribute to PMR upon transfer to females during copulation. We found that Acp26 Aa, CG1656 and CG42474 were significantly downregulated in Wolbachia-infected males, whereas SP, CG1652, CG9997, and CG17575 showed no significant difference between Wolbachia-infected and uninfected males. These results suggest that by decreasing the expression of some Sfps in the male hosts,Wolbachia may modulate sexual conflicts of their insect hosts in a way favoring females,thus benefit their own transmission through host populations. This study provides new insights into the host-endosymbiont interaction,which may support the application of endosymbionts for the control of pests and disease vectors.
摘要：
Background: Major depressive disorder (MDD) is a severe mental illness that affects more than 350 million people worldwide. However, the molecular mechanisms of depression are currently unclear. Studies suggest that Drosophila and humans have similar depression-like symptoms under pressure. In this research, we choose Drosophila melanogaster as the animal model to explore the molecular mechanisms that trigger depression. Results: We found that feeding D. melanogaster with the medium containing Levodopa or Chlorpromazine could induce depression-like phenotypes in both behavioral and biochemical biomarkers, including significantly decreased food intake, mating frequency, serotonin (5-HT) concentration, and increased malondialdehyde (MDA) concentration as well as reduced activity of superoxide dismutase (SOD). Moreover, the progeny of Chlorpromazine-treated flies also showed these depression-like features. By RNA-seq technology, we identified 467 genes that were differentially expressed between Chlorpromazine treated (CPZ) and control male flies [fold change of >= 2 (q-value < 5%)]. When comparing CPZ with control flies, 312 genes were upregulated and 155 genes downregulated. Differential expression of genes related to metabolic pathway, Parkinson's disease, Huntington's disease, Alzheimer's disease and lysozyme pathways were observed. Quantitative reverse transcriptase PCR (qRT-PCR) confirmed that 19 genes are differentially expressed in CPZ and control male flies. Conclusions: Levodopa, or Chlorpromazine can induce depression-like phenotypes in D. melanogaster regarding changes of appetite and sexual activity, and some key biochemical markers. A total of 467 genes were identified by RNA-seq analysis to have at least a 2-fold-change in expression between CPZ and control flies, including genes involved in metabolism, neurological diseases and lysozyme pathways. Our data provide additional insight into molecular mechanisms underlying depressive disorders in humans and may also contribute to clinical treatment.
