期刊：
Alzheimer's & Dementia: Translational Research & Clinical Interventions,2019年5(1):671-684 ISSN：2352-8737
通讯作者：
Tong, Z.
作者机构：
[Chunli Duan; Ge Gao; Hui Yang] Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing, China;[Dehua Cui; Jun Yang; Aibo Wang; Rui Wang] Department of Radiology, Peking University Third Hospital, Key Laboratory of Magnetic Resonance Imaging Equipment and Technique, Beijing, China;Corresponding author. Tel: +86-010-83950362;Fax: +86-010-83950363.;∗Corresponding author. Tel: 604-822-8019
通讯机构：
[Weihong Song; Hongbin Han] D;[Zhiqian Tong] L;Department of Radiology, Peking University Third Hospital, Key Laboratory of Magnetic Resonance Imaging Equipment and Technique, Beijing, China<&wdkj&>Corresponding author. Tel: +86-010-83950362;Fax: +86-010-83950363.<&wdkj&>∗Corresponding author. Tel: 604-822-8019;Fax: 604-822-7981.<&wdkj&>∗∗Corresponding author. Tel: +86-010-82266972
关键词：
Red light (RL);Alzheimer's disease (AD);Amyloid β (Aβ);Formaldehyde (FA);Formaldehyde dehydrogenase (FDH);Extracellular space (ECS);Interstitial fluid (ISF)
摘要：
Introduction: Pharmacological therapies to treat Alzheimer's disease (AD) targeting "Abeta" have failed for over 100 years. Low levels of laser light can disassemble Abeta. In this study, we investigated the mechanisms that Abeta-blocked extracellular space (ECS) induces memory disorders in APP/PS1 transgenic mice and addressed whether red light (RL) at 630 nm rescues cognitive decline by reducing Abeta-disturbed flow of interstitial fluid (ISF). Methods: We compared the heating effects on the brains of rats illuminated with laser light at 630, 680, and 810 nm for 40 minutes, respectively. Then, a light-emitting diode with red light at 630 nm (LED-RL) was selected to illuminate AD mice. The changes in the structure of ECS in the cortex were examined by fluorescent double labeling. The volumes of ECS and flow speed of ISF were quantified by magnetic resonance imaging. Spatial memory behaviors in mice were evaluated by the Morris water maze. Then, the brains were sampled for biochemical analysis. Results: RL at 630 nm had the least heating effects than other wavelengths associated with ~49% penetration ratio into the brains. For the molecular mechanisms, Abeta could induce formaldehyde (FA) accumulation by inactivating FA dehydrogenase. Unexpectedly, in turn, FA accelerated Abeta deposition in the ECS. However, LED-RL treatment not only directly destroyed Abeta assembly in vitro and in vivo but also activated FA dehydrogenase to degrade FA and attenuated FA-facilitated Abeta aggregation. Subsequently, LED-RL markedly smashed Abeta deposition in the ECS, recovered the flow of ISF, and rescued cognitive functions in AD mice. Discussion: Abeta-obstructed ISF flow is the direct reason for the failure of the developed medicine delivery from superficial into the deep brain in the treatment of AD. The phototherapy of LED-RL improves memory by reducing Abeta-blocked ECS and suggests that it is a promising noninvasive approach to treat AD.
摘要：
A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterium, strain Ra1(T), was isolated from the gut of a wood-feeding lower termite, Reticulitermes aculabialis. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain was closely related to Chryseobacterium rigui JCM 18078(T) (96.7% similarity). Growth was observed at 15-45 degrees C (optimum 30 degrees C), at pH 6.0-9.0 (optimum pH 8.0) and in the presence of 0-2% (w/v) NaCl (optimum 0 %). The DNA G+C content of strain Ra1(T) was 39.9 mol%. Cells contained menaquinone MK-6 as the sole respiratory quinone and the major fatty acids were iso-C-15 (:) (0), iso-C-17 : 0, summed feature 3 (comprising C(16 : 1 omega)6c and/or C-16 : 1 omega 7c) and summed feature 9 (comprising C-16 : 0 10-methyl and/or iso-C-17 : (1)omega 9c). The predominant polyamine was sym-homospermidine. The cellular polar lipids consisted of one phosphatidylethanolamine, three unidentified aminolipids, one unidentified phospholipid and one unidentified lipid. Based on phenotypic, genotypic and phylogenetic studies, it is concluded that strain Ra1(T) represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium reticulitermitis sp. nov. is proposed. The type strain is Ra1(T) (=CCTCC AB 2015431(T) = KCTC 52230(T)).
作者：
Yang, Shu Yan;Zheng, Ying;Huang, Zhou;Wang, Xue Min;Yang, Hong*
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2016年66(1):518-522 ISSN：1466-5026
通讯作者：
Yang, Hong
作者机构：
[Yang, Hong; Yang, Shu Yan; Wang, Xue Min; Zheng, Ying] Cent China Normal Univ, Sch Life Sci, Inst Entomol, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Huang, Zhou] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China.;[Huang, Zhou] Chinese Acad Sci, Grad Sch, Beijing M19T, Peoples R China.
通讯机构：
[Yang, Hong] C;Cent China Normal Univ, Sch Life Sci, Inst Entomol, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
会议名称：
第七届全国微生物资源学术暨国际微生物系统与分类学研讨会
会议时间：
2015-8-25
会议地点：
杭州
会议主办单位：
中国微生物学会;国家微生物资源平台
会议论文集名称：
第七届全国微生物资源学术暨国际微生物系统与分类学研讨会论文集
摘要：
Bacterial strain M 19T was isolated from the gut of a wood-feeding termite,Nasutiterraes hainanensis.Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M19T was related to members of the genus Lactococcus,with sequence similarities ranging from 84.8 to 95.5%.The isolate was Gram-staining-positive,catalase-negative,non-motile.Cells were coccoid or ovoid-shaped,and occurred singly,in pairs or as short chains.Growth of strain M19T occurred at 10-40℃,and at pH 5.0-7.5.The DNA G+C content of strain M19 was 39.6 mol%and the major fatty acids were C16:0(43.59%),cyclo-C19:0w8c(13.08),C18:1w9c(9.08%),summed feature 7(10.13%)and summed feature 8(9.46%).Based on the phylogenetic,chemotaxonomic and phenotypic data presented,strain M19T represents a novel species of the genus Lactococcus,for which the name Lactococcus nasutitermitis sp.nov.is proposed.The type swain is M19 T(=CGMCC 1.15204).
摘要：
Termites are among the few arthropods that emit methane to the atmosphere, which is a significant source of global greenhouse gas due to their huge biomass on earth. In this study, phylogenetic diversity of Archaea of five termite species from different lineages were analyzed based on 16S rRNA genes. Archaea associated with wood-feeding lower termite, R. chinensis were exclusively Methanobrevibacter in the order Methanobacteriales. This type of methanogens was also found in Nasutitermes sp. and Microcerotermes sp. but not in the fungus-cultivating termites, Odontotermes formosanus and Macrotermes barneyi, which harbor Archaea of the order Methanoplasmatales and Methanosarcinales in their guts. Archaeal diversity of wood-feeding higher termites was higher than wood-feeding lower termites. The highest archaeal diversity was found in Nasutitermes sp. In addition to methanogens affiliated with the orders Methanobacteriales, Methanomicrobiales, and Methanoplasmatales, 37% of archaeal clones were affiliated with non-methanogenic Thaumarchaeota. The results of this study will be significant for further understanding of symbiotic relationship between intestinal microbiota and termites.
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2015年65(1):85-89 ISSN：1466-5026
通讯作者：
Yang, Hong
作者机构：
[Yang, Hong; Lv, Wanyu; Fang, Hao] Cent China Normal Univ, Inst Entomol, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Huang, Zhou; Liu, Shuang-Jiang] Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing 100101, Peoples R China.;[Huang, Zhou] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China.
通讯机构：
[Yang, Hong] C;Cent China Normal Univ, Inst Entomol, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
摘要：
Strain TS-56(T) was isolated from the gut of a wood-feeding termite, Reticulitermes chinensis Snyder. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the strain represented a member of the genus Gryllotalpicola of the family Microbacteriaceae, with sequence similarities to other species of the genus ranging from 96.6 % to 97.8 %. The isolate was Gram-stain-positive, non-motile, with light yellow colonies and irregular short rod-shaped cells (0.4-0.6 microm in diameter, 0.6-1.0 microm in length). Growth of TS-56(T) occurred at 20-35 degrees C (optimum, 30 degrees C) and at pH 4.0-8.0 (optimum, pH 5.0). The peptidoglycan of TS-56(T) contained ornithine, glutamic acid, alanine, homoserine and glycine. The acyl type was acetyl. The most abundant cellular fatty acid of TS-56(T) was cyclohexyl-C17 : 0 (88.79 %). The respiratory menaquinone was MK-11. The polar lipid profile contained disphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and two unknown glycolipids. DNA of the type strain had a G+C content of 67.4 mol%. On the basis of the phylogenetic properties and phenotypic distinctiveness, TS-56(T) represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola reticulitermitis sp. nov. is proposed. The type strain is TS-56(T) ( = CGMCC 1.10363(T) = NBRC 109838(T)).
