作者机构：
Wuhan University of Science and Technology, Wuhan 430080, PR China;School of Life Science, Central China Normal University, Wuhan 430079, PR China
会议名称：
第十届室内空气品质和质量国际学术会议(Proceedings of the 10th International Conference on Indoor Air Quality and Climate)
摘要：
Objective: To study the effect of formaldehyde on DNA-protein crosslinks (DPC) in buccal cells in students who were taking anatomy course. Methods: The modified KCl-SDS precipitation assay was applied. This method has been used to explore DPC induced by different pollutants in laboratory studies. Results: 37 students in medical school and 40 in school of sciences were studied. The frequency of exposure to formaldehyde was 6 h per week in exposed group. Concentrations of formaldehyde in anatomy laboratory ranged from 0.42 to 1.57 mg/m. Exposure to formaldehyde resulted in an increase of DNA-protein crosslinks. DNA-protein crosslinks in exposed and nonexposed students were 25.72±6.48％ and 22.88±5.34％ (p＜0.05), respectively. Air formaldehyde caused a significant increase in DNA-protein crosslinks of the females in two groups (27.72±5.76％ and 22.29±4.20％,p＜0.01). However, there was no difference in males (24.02±6.72 and 23.48±6.33, p＞0.05). Conclusions: The results suggest that exposure to formaldehyde in medical students increase the frequency of DNA-protein crosslinks in buccal cells. Our results also indicate that females may be more sensitive to formaldehyde exposure.
摘要：
<正>白蚁是自然界能够降解纤维素的为数不多的昆虫之一,其对纤维素的降解与肠道内丰富的共生微生物密切相关。本文报道了以广泛分布于我国的一种低等木食性白蚁,黑胸散白蚁(Reticulitermes Chinese Snyder)为模式生物,对白蚁肠道内共生微生物的多样性和功能的初步研究结果。以核糖体小亚基18S rRNA为分子指针对黑胸散白蚁肠道内鞭毛虫的系统发育分析表明,该白蚁体内鞭毛虫主要有6种,
作者：
Du, X. Z.;Zhou, L.;Zhao, H. B.;Wang, Y. F.*;Gui, J. F.
期刊：
Fish Physiology and Biochemistry,2008年34(2):175-184 ISSN：0920-1742
通讯作者：
Wang, Y. F.
作者机构：
[Zhao, H. B.; Du, X. Z.; Wang, Y. F.; Zhou, L.] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Gui, J. F.] Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
通讯机构：
[Wang, Y. F.] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
Hir/Hira (histone regulation) genes were first identified in yeast as negative regulators of histone gene expression. It has been confirmed that HIRA is a conserved family of proteins present in various animals and plants. In this paper, the cDNAs of the Hira homolog named CagHira and CaHira were isolated from gynogenetic gibel carp (gyno-carp) and gonochoristic color crucian carp (gono-carp) respectively. The full-length CagHira is 3,860 bp in length with an open reading frame (ORF) of 3,033 bp that encodes 1,011 amino acids, while the full-length CaHira is 3,748 bp in length and also has an ORF of 3,033 bp. The deduced amino acid sequences of both Hira homologs contain seven WD domains and show high identity with other HIRA family members. RT-PCR analyses revealed strong expression of Hira in the ovaries, whereas no expression was detected in the testes of either of the fishes. Hira transcription was not detected in the liver of gyno-carp, but a high level of Hira mRNA was observed in gono-carp. The temporal expression pattern showed that the Hira mRNA is consistently expressed during all embryonic development stages in gyno-carp. However, the abundance of CaHira mRNA significantly decreased (P < 0.05) shortly after fertilization and then increased again and remained stable from gastrula till hatching. The varying spatiotemporal expression patterns of Hira genes in gyno-carp and gono-carp may be associated with the differing reproductive modes used by these two closely related fishes. Our results suggest that Hira may play a role not only in the decondensation of sperm nucleus and the formation of pronucleus during fertilization, but also in gastrulation and the subsequent development of embryos.
