Comparison of pesticide sensitivity by electrochemical test based on acetylcholinesterase biosensor
作者：
Du, Dan
* ;Huang, Xi;Cai, He;Zhang, Aidong
（ 期刊：
Biosensors and Bioelectronics ,2007年23(2):285-289 ISSN：0956-5663
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minist Educ, Key Lab Pest & Chem Biol, Wuhan 430079, Peoples R China.;Wuhan Iron & Steel Co, Ctr Technol, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pest & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Biosensor;Electrochemical test;Immobilization;Pesticide sensitivity
摘要：
Based on the change in electrochemical behavior of enzymatic activity induced by pesticide, a novel electrochemical method has been devised for investigation of pesticide sensitivity using acetylcholinesterase (AChE) biosensor. Because of the excellent biocompatibility and good stability of chitosan matrix, it prevented leakage of the AChE from electrode. Multiwall carbon nanotube (MWNT) promoted electron transfer reaction at a lower potential and catalyzed the electro-oxidation of thiocholine, thus amplifying the sensitivity and amperometric response of the biosensor. Four pesticides of carbaryl, malathion, dimethoate and monocrotophos were selected to discuss their inhibition efficiencies to AChE. The inhibition curves were similar to Michealis-Menten and the Michealis-Menten constants (Km) were calculated to be 0.96 μM, 1.78 μM, 1.97 μM and 4.28 μM, respectively. Ninety-five percent reactivation of the inhibited AChE could be regenerated using pralidoxime iodide within 8 min. The proposed electrochemical pesticide sensitivity test exhibited high sensitivity, low cost and simplified procedures, which is a promising new tool for comparison of pesticide sensitivity and for selection of the most efficient enzyme inhibitors. © 2007 Elsevier B.V. All rights reserved.
语种：
英文
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One-step electrochemically deposited interface of chitosan–gold nanoparticles for acetylcholinesterase biosensor design
作者：
Du, Dan
* ;Ding, Jiawang;Cai, Jie;Zhang, Aidong
（ 期刊：
Journal of Electroanalytical Chemistry ,2007年605(1):53-60 ISSN：1572-6657
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Wuhan Iron & Steel Co, Ctr Technol, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Biosensor;Chitosan;Electrodeposition;Gold nanoparticles;Organophosphate pesticides
摘要：
A novel interface embedded in situ gold nanoparticles (GNPs) in chitosan hydrogel was constructed by one-step electrochemical deposition in solution containing tetrachloroauric (III) acid and chitosan. This deposited interface possessed excellent biocompatibility and good stability by characterization of attenuated total reflection Fourier-transform infrared spectra (ATR-FTIR), atomic force microscopy (AFM) and UV-vis spectra. The immobilized AChE, as a model, showed excellent activity to its substrate and provided a quantitative measurement of organophosphate pesticides involved in the inhibition action. Operational parameters, including the deposition time, tetrachloroauric (III) acid concentration have been optimized. Under the optimal electrodeposition, an amperometric sensor for the fast determination of malathion and monocrotophos, respectively was developed with detection limit of 0.001 μg mL-1. The simple method showed good fabrication reproducibility and acceptable stability, which provided a new avenue for electrochemical biosensor design. © 2007 Elsevier B.V. All rights reserved.
语种：
英文
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Reagentless amperometric carbohydrate antigen 19-9 immunosensor based on direct electrochemistry of immobilized horseradish peroxidase
作者：
Du Dan
* ;Xu Xiaoxing;Wang Shengfu;Zhang Aidong
（ 期刊：
Talanta ,2007年71(3):1257-1262 ISSN：0039-9140
通讯作者：
Du Dan
作者机构：
[Du Dan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Changshu Inst Technol, Dept Chem, Changshu 215500, Peoples R China.;Minist Educ, Key Lab Synth & Applicat Organ Funct Mol, Wuhan 430062, Peoples R China.
通讯机构：
[Du Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Carbohydrate antigen 19-9 (CA19-9);Colloidal gold nanoparticles;Electrochemical sensors;Enzyme labeled antibody;Immunosensor
摘要：
A reagentless immunosensor for rapid determination of carbohydrate antigen 19-9 (CA19-9) in human serum was proposed. This strategy was based on the immobilization of antibody in colloidal gold nanoparticle modified carbon paste electrode and the direct electrochemistry of horseradish peroxidase (HRP) that was labeled to a CA19-9 antibody. The nanoparticles were efficient for preserving the activity of immobilized biomolecules. Thus, the immobilized HRP displayed its direct electrochemistry with a rate constant of 1.02 s<sup>-1</sup>. The incubation of the immunosensor in phosphate buffer solution (PBS) including CA19-9 antigen leading to the formation of antigen-antibody complex, which made the block of electron transfer of HRP toward electrode and resulted in significant peak current decrease of HRP. Under the optimal conditions, the current decrease was proportional to CA19-9 concentrations ranging from 2 to 30 U/ml with a detection limit of 1.37 U/ml at a current decrease by 10%. The immunosensor showed an acceptable accuracy compared with those obtained from immunoradiometric assays, with intra-assay coefficient of 7.3 and 6.9% at CA19-9 concentrations of 5 and 15 U/ml, respectively, and inter-assay coefficient of 9.6% at a CA19-9 concentration of 20 U/ml. The storage stability was acceptable in a pH 7.0 PBS at 4 °C for 10 days. This method avoids the addition of electron transfer mediator, thus simplifies the immunoassay procedure and decreases the analytical time. It provides a new promising platform for clinical immunoassay. ©2006 Elsevier B.V. All rights reserved.
语种：
英文
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An amperometric acetylthiocholine sensor based on immobilization of acetylcholinesterase on a multiwall carbon nanotube-cross-linked chitosan composite
作者：
Du, Dan
* ;Huang, Xi;Cai, Jie;Zhang, Aidong
（ ;Ding, Jiawang;...
期刊：
Analytical and Bioanalytical Chemistry ,2007年387(3):1059-1065 ISSN：1618-2642
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minst Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Technol Ctr Wuhan Iron & Steel Co, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minst Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Acetylthiocholine sensor;Chitosan;Composite film;Multiwall carbon nanotube
摘要：
A simple method has been devised for immobilization of acetylcholinesterase (AChE)-covalent bonding to a multiwall carbon nanotube (MWNT)-cross-linked chitosan composite (CMC)-and a sensitive amperometric sensor for rapid detection of acetylthiocholine (ATCl) has been based on this. Fourier-transform infrared spectroscopy proved that the native structure of the immobilized enzyme was preserved on this chemically clean and homogeneous composite film, because of the excellent biocompatibility and non-toxicity of chitosan. Glutaraldehyde was used as cross-linker to covalently bond the AChE, and efficiently prevented leakage of the enzyme from the film. Because of the inherent conductive properties of the MWNT, the immobilized AChE had greater affinity for ATCl and excellent catalytic effect in the hydrolysis of ATCl, with a $$K^{{{\text{app}}}}_{{\text{m}}} $$ value of 132 μmol L-1, forming thiocholine, which was then oxidized to produce a detectable and rapid response. Under optimum conditions the amperometric current increased linearly with the increasing concentration of ATCl in the range 2.0-400 μmol L-1, with a detection limit of 0.10 μmol L-1. Fabrication reproducibility of the sensor was good and the stability was acceptable. The sensor is a promising new tool for characterization of enzyme inhibitors and for pesticide analysis. [Figure not available: see fulltext.]. © Springer-Verlag 2007.
语种：
英文
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具有自组装功能的氨基甲酸酯类化合物的合成
期刊：
化学试剂 ,2007年29(7):389-390,393 ISSN：0258-3283
作者机构：
[张爱东; 涂海洋; 祝丹华; 程桂红] 华中师范大学
关键词：
1,6-己二胺;硫辛酸;异氰酸甲酯;氨基甲酸酯
摘要：
以1,6-己二胺、对羟基苯甲酸、硫辛酸、异氰酸甲酯为原料,合成了具有自组装功能的氨基甲酸酯类化合物,并通过IR、1HNMR、MS对化合物进行了确证.
语种：
中文
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基于多壁碳纳米管/壳聚糖膜修饰的酶传感器研究
作者机构：
[黄茜; 杜丹] 华中师范大学农药与化学生物学教育部重点实验室
会议名称：
中国化学会第九届分析化学年会暨全国原子光谱学术会议
会议时间：
2006-10-10到2006-10-11
会议地点：
南昌
会议论文集名称：
南昌大学学报理科版
关键词：
乙酰胆碱酯酶;壳聚糖;多壁碳纳米管;传感器;电化学
摘要：
本文将 AChE 固定于多壁碳纳米管/壳聚糖复合膜修饰玻碳电极表面,采用电化学方法定量测定了水相中 ATC(?)的浓度,利用循环伏安法表征了氨基甲酸酯类农药西维因对酶活性的抑制作用。在最优条件下测定峰电流与 ATC(?)浓度在2.0～400 μM范围内呈现良好的线性关系,检测限为0.80 μM。修饰电极具有良好的重现性和稳定性。
语种：
中文
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Immobilization of acetylcholinesterase on gold nanoparticles embedded in sol–gel film for amperometric detection of organophosphorous insecticide
作者：
Du, Dan
* ;Chen, Shizhen;Cai, Jie;Zhang, Aidong
（ 期刊：
Biosensors and Bioelectronics ,2007年23(1):130-134 ISSN：0956-5663
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Wuhan Iron & Steel Co, Ctr Technol, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Amperometric sensor;Gold nanoparticles;Monocrotophos;Sol-gel
摘要：
A simple method to immobilize acetylcholinesterase (AChE) on silica sol-gel (SiSG) film assembling gold nanoparticles (AuNPs) was proposed, thus a sensitive, fast and stable amperometric sensor for quantitative determination of organophosphorous insecticide was developed. The large quantities of hydroxyl groups in the sol-gel composite provided a biocompatible microenvironment around enzyme molecule and stabilized its biological activity to a large extent. The immobilized AChE could catalyze the hydrolysis of acetylthiocholine chloride (ATCl) with a Kmapp value of 450 μM to form thiocholine, which was then oxidized to produce detectable single with a linear range of 10-1000 μM. AuNPs catalyzed the electro-oxidation of thiocholine, thus increasing detection sensitivity. Based on the inhibition of organophosphorous insecticide on the enzymatic activity of AChE, using monocrotophos as a model compound, the conditions for detection of the insecticide were optimized. The inhibition of monocrotophos was proportional to its concentration ranging from 0.001 to 1 μg/ml and 2 to 15 μg/ml, with the correlation coefficients of 0.9930 and 0.9985, respectively. The detection limit was 0.6 ng/ml at a 10% inhibition. The developed biosensor exhibited good reproducibility and acceptable stability, thus providing a new promising tool for analysis of enzyme inhibitors. © 2007 Elsevier B.V. All rights reserved.
语种：
英文
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Ligand exchange of manganese(II) macrocyclic pentaamine complexes: Synthesis, characterization, and single-crystal X-ray structure determination
作者：
Tu, Hai-Yang;Liu, Ping;Wang, Cheng-Jin;Gao, Meng-Xiang;Zhang, Ai-Dong
* （ 期刊：
Molecular Crystals and Liquid Crystals ,2007年469(1):79-87 ISSN：1542-1406
通讯作者：
Zhang, Ai-Dong
（ 作者机构：
[Zhang, Ai-Dong; Gao, Meng-Xiang; Liu, Ping; Wang, Cheng-Jin; Zhang, AD; Tu, Hai-Yang] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Zhang, Ai-Dong] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
ligand exchange;manganese(II);X-ray crystallography
摘要：
[Mn(C23H34ClN5)] (manganese(II) {24-chloro-2,21-dimethyl-(4R,9R,14R,19R)-3,10,13,20,26-pentaazatetracyclo[20.3.1.04,9.014,19]hexacosa-1(26),2(3),20(21),22(23),24-pentaene}, ([Mn(PAHP)]) has been synthesized and characterized by FT-IR spectroscopy, UV-vis, and MS spectra. Single crystals of [Mn(CH3CH2OH)Cl(C23H34ClN5)]Cl·H2O and [Mn(H2O)2(C23H34ClN5)]Cl2·CH2Cl2 were obtained by slow evaporation from ethanol-dichloromethane and 1,2-dichloromethane-DMF solution, respectively. X-ray crystallographic study of the complex reveals that the chloride ligand on manganese(II) can be substituted by oxygen-containing ligands, such as methanol.
语种：
英文
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以自组装单层为模型研究生物材料表面化学和生物相容性的关系
作者：
罗祥林（综述）;邱笛（综述）;罗娟（综述）;王立坚 （综述）;张爱东（审校）
（ 期刊：
生物医学工程学杂志 ,2007年24(3):697-700 ISSN：1001-5515
通讯作者：
Luo, X.
作者机构：
[罗祥林（综述）; 邱笛（综述）; 罗娟（综述）; 王立坚 （综述）] 四川大学,高分子科学与工程学院;[张爱东（审校）] 华中师范大学,化学学院
关键词：
生物材料;生物相容性;表面化学性质;自组装单层;模型
摘要：
生物材料表面性质对生物相容性而言是至关重要的.生物材料表面组成和结构的复杂性,使得人们很难确切弄清楚材料表面的化学性质和结构是怎样控制表面与蛋白质、细胞间的生物学反应.自组装单层(Self-assembled monolayers,SAMs)具有表面结构确定、表面化学性质能系统性改变的特点,而且可以用多种表征手段定性或定量确定自组装单层本身及同蛋白质、细胞的作用,因而成为研究生物材料表面化学性质与生物相容性关系的合适模型.
语种：
中文
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化学专业词汇记忆方法探讨
期刊：
科教文汇(下旬刊) ,2007年(11):227 ISSN：1672-7894
作者机构：
华中师范大学化学学院,湖北·武汉,430079
关键词：
双语学习;化学;词汇记忆
摘要：
本文根据化学专业词汇数量大,记忆困难的实际情况,结合大学双语课程教与学的经验与体会,探讨了化学双语学习中专业词汇的记忆方法。
语种：
中文
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Amperometric Detection of Triazophos Pesticide Using Acetylcholinesterase Biosensor Based on Multiwall Carbon Nanotube-Chitosan Matrix
作者：
Dan DU;Xi HUANG;Aidong ZHANG
（ 作者机构：
[Aidong ZHANG; Dan DU; Xi HUANG] Key Laboratory of Pesticide & Chemical Biology of Ministry of Education,Central China Normal University,Wuhan 430079,P.R.China
会议名称：
The Eleventh International & The First Sino-Japan Bilateral Symposium on Electroanalytical Chemistry (11th ISEC & 1st SJBSEC)第十一届国际电分析化学学术会议暨第一届中日双边电分析化学学术会议
会议时间：
2007-8-16
会议地点：
长春
会议主办单位：
中国化学会
会议论文集名称：
The Eleventh International & The First Sino-Japan Bilateral Symposium on Electroanalytical Chemistry (11th ISEC & 1st SJBSEC)第十一届国际电分析化学学术会议暨第一届中日双边电分析化学学术会议论文集
语种：
英文
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Determination of carbaryl pesticide using amperometric acetylcholinesterase sensor formed by electrochemically deposited chitosan
作者：
Du, Dan
* ;Ding, Jiawang;Cai, Jie;Zhang, Aidong
（ 期刊：
Colloids and Surfaces B: Biointerfaces ,2007年58(2):145-150 ISSN：0927-7765
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Wuhan Iron & Steel Co, Ctr Technol, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Amperometric sensor;Carbaryl;Chitosan;Electrochemical deposition
摘要：
A sensitive, fast and cheap sensor for quantitative determination of carbaryl pesticide using amperometric acetylcholinesterase (AChE) sensor based on electrochemically deposited chitosan was reported. From a mildly acidic chitosan solution, a chitosan film is electrochemically deposited on Au electrode surface via a negative voltage bias, leading to a stable AChE sensor. The characteristics of the deposited layer were observed to be dependent upon the deposition time, pH, and the chitosan concentration. Fourier-transform infrared spectra proved that the immobilized enzyme could preserve their native structure due to the excellent biocompatibility and non-toxicity of chitosan. Under the optimal experimental conditions, the carbaryl inhibition on AChE-CHIT/Au was proportional to its concentration in two ranges, from 0.005 to 0.1 μg/ml and 0.5 to 5 μg/ml, with the correlation coefficients of 0.9966 and 0.9982, respectively. The detection limit was 0.003 μg/ml taken as the concentration equivalent to a 10% decrease in signal. The determination of carbaryl in garlic samples obtained from export of farm base showed acceptable accuracy. The developed sensor exhibited good fabrication reproducibility and acceptable stability, which provided a new promising tool for pesticide analysis. © 2007 Elsevier B.V. All rights reserved.
语种：
英文
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Amperometric detection of triazophos pesticide using acetylcholinesterase biosensor based on multiwall carbon nanotube–chitosan matrix
作者：
Du, Dan
* ;Huang, Xi;Cai, Jie;Zhang, Aidong
（ 期刊：
Sensors and Actuators B-Chemical ,2007年127(2):531-535 ISSN：0925-4005
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430070, Peoples R China.;Technol Ctr Wuhan Iron & Steel Co, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430070, Peoples R China.
关键词：
acetylcholinesterase;biosensor;chitosan;multiwall carbon nanotube;triazophos
摘要：
A simple method for immobilization of acetylcholinesterase (AChE) on multiwall carbon nanotubes (MWNTs)-chitosan (MC) composite was proposed and thus a sensitive, fast and stable amperometric, sensor for quantitative determination of organophosphorous insecticide was developed. Atomic force microscopy showed that this matrix possessed homogeneously netlike structure, which prevented enzyme from leaving out of the electrode. MWNTs promoted electron transfer reactions at a lower potential and catalyzed the electro-oxidation of thiocholine, thus increasing detection sensitivity. Based on the inhibition of organophosphorous insecticide to the enzymatic activity of AChE, using triazophos as a model compound, the conditions for detection of the insecticide were explored. Under optimal conditions, the inhibition of triazophos was proportional to its concentration in two ranges, from 0.03 to 7.8 mu M and 7.8 to 32 mu M with a detection limit of 0.01 mu M. A 95% reactivation of the inhibited AChE could be regenerated for using pralidoxime iodide within 8 min. The constructed biosensor processing prominent characteristics and performance such as good precision and reproducibility, acceptable stability and accuracy, fast response and low detection limit has potential application in the characterization of enzyme inhibitors and detection of toxic compounds against to enzyme. (c) 2007 Elsevier B.V. All rights reserved.
语种：
英文
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A novel detection strategy for odorant molecules based on controlled bioengineering of rat olfactory receptor I7
作者：
Hou, Yanxia
* ;Jaffrezic-Renault, Nicole;Martelet, Claude;Zhang, Aidong
（ ;Minic-Vidic, Jasmina;...
期刊：
Biosensors and Bioelectronics ,2007年22(7):1550-1555 ISSN：0956-5663
通讯作者：
Hou, Yanxia
作者机构：
[Hou, Yanxia] Ecole Cent Lyon, Ctr Genie Elect Lyon, CEGELY, F-69134 Ecully, France.;Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.;INRA, NOPA, RCC, F-78352 Jouy En Josas, France.;Univ Lecce, Dipartimento Ingn Innovaz, I-73100 Lecce, Italy.;CNR, INFM, Natl Nanotechnol Lab, I-73100 Lecce, Italy.
通讯机构：
[Hou, Yanxia] E;Ecole Cent Lyon, Ctr Genie Elect Lyon, CEGELY, F-69134 Ecully, France.
关键词：
Biosensor;Electrochemical impedance spectroscopy;G protein-coupled receptors;Olfactory receptor I7;Self-assembled multilayer
摘要：
In this study, we report a dose-dependent detection of odorant molecules in solution by rat olfactory receptor I7 (OR I7) in its membrane fraction. The OR I7 is immobilized on a gold electrode by multilayer bioengineering based on a mixed self-assembled monolayer and biotin/avidin system, which allows for a well-controlled immobilization of the bioreceptor within its lipid environment. The odorant detection is electronically performed in a quantitative manner by electrochemical impedance spectroscopy (EIS) measurements on samples and controls. © 2006 Elsevier B.V. All rights reserved.
语种：
英文
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化学专业词汇记忆方法探讨
期刊：
科协论坛(下半月) ,2007年(07):146 ISSN：1007-3973
作者机构：
华中师范大学化学学院;华中师范大学化学学院 湖北·武汉430079
摘要：
<正>化学专业词汇量大,专业性强,可分为专业术语和化合物名称两部分。这类词汇与生活联系少,记忆较困难。但其中仍有规律可循,掌握了这些规律,可起到事半功倍的效果。1专业术语的记忆:
语种：
中文
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基于乙酰胆碱酯酶传感器的农药药效检测新方法
作者机构：
[张爱东; 陈世桢; 杜丹] 农药与化学生物学教育部重点实验室,华中师范大学
会议名称：
中国化学会第十四届有机分析及生物分析学术研讨会
会议时间：
2007-10-01
会议地点：
武汉
会议论文集名称：
中国化学会第十四届有机分析及生物分析学术研讨会论文集
关键词：
乙酰胆碱酯酶;电化学检测;金纳米;农药药效;药效检测;玻碳电极;循环伏安法
摘要：
本文将乙酰胆碱酯酶(AChE)固定于金纳米/溶胶凝胶复合膜修饰的玻碳电极表面,利用循环伏安法表征了3种不同的有机磷农药以及氨基甲酸酯类农药西维因对酶活性的抑制作用,并评价了这3种农药的灵敏度。
语种：
中文
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Electrochemical thiocholine inhibition sensor based on biocatalytic growth of Au nanoparticles using chitosan as template
作者：
Du, Dan
* ;Ding, Jiawang;Cai, Jie;Zhang, Aidong
（ 期刊：
Sensors and Actuators B-Chemical ,2007年127(2):317-322 ISSN：0925-4005
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Key Lab Pesticide & Chem Biol Minist Educ, Wuhan 430079, Peoples R China.;Technol Ctr Wuhan Iron & Steel Co, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol Minist Educ, Wuhan 430079, Peoples R China.
关键词：
acetylcholinesterase;biocatalytic growth;electrochemical sensor;electrodeposition;gold nanoparticles
摘要：
A simple strategy for the design of an electrochemical sensor based on enzyme-induced growth of gold nanoparticles (AuNPs) without adding gold nano-seeds was proposed with acetylcholinesterase (AChE) as a model. Owing to the excellent biocompatibility of chitosan film for the subsequent assembly of biological molecules, chitosan film was first electrochemically deposited on Au electrode surface from a mildly acidic chitosan solution via a negative voltage bias. Based on immobilization of AChE, a stable AChE sensor (AChE-CS/Au) was constructed. The immobilized AChE mediated hydrolysis of ATCl and yielded a reducing agent thiocholine. In the presence of AuCl4-, AuNPs were formed without addition of any metal nanoparticles. Atomic force microscopy (AFM) images proved that small spherical nanoparticles could grow on the enzyme-modified surface after incubation in HAuCl4 and ATCl solution. [Fe(CN)(6)](3-/4-) redox system was used as a probe to indicate the process of electron transfer across the interface and also analyze the enzyme inhibitor quantitatively. The inhibition of malathion on AChE was proportional to its concentration in the range of 0.1-500 ng ml(-1) (R= 0.9989), with detection limit of 0.03 ng ml(-1). (c) 2007 Elsevier B.V All rights reserved.
语种：
英文
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Rapid determination of triazophos using acetylcholinesterase biosensor based on sol-gel interface assembling muldwall carbon nanotubes
作者：
Du, Dan
* ;Cai, Jie;Song, Dandan;Zhang, Aidong
（ 期刊：
Journal of Applied Electrochemistry ,2007年37(8):893-898 ISSN：0021-891X
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Technol Ctr Wuhan Iron & Steel Co, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Amperometric determination;Biosensor;Multiwall carbon nanotube;Sol-gel;Triazophos
摘要：
A sensitive and stable amperometric sensor has been devised for rapid determination of triazophos based on efficient immobilization of acetylcholinesterase (AChE) on silica sol-gel (SiSG) film assembling multiwall carbon nanotubes (MWNTs). The sol-gel matrix provided a biocompatible microenvironment around the enzyme and efficiently prevented leakage of the enzyme from the film. In the presence of acetylthiocholine chloride (ATCl) as a substrate, MWNTs promoted electron transfer reactions at a lower potential and catalyzed electrochemical oxidation of enzymatically formed thiocholine, thus increasing detection sensitivity. Based on the inhibition of organophosphorous compound on the enzymatic activity of AChE, using triazophos as a model compound, the effects of pH, temperature, and MWNTs contents were explored. Under optimum conditions, the inhibition of triazophos was proportional to its concentration from 0.02 μM to 1 μM and from 5 μM to 30 μM, with a detection limit of 0.005 μM. The determination of triazophos in garlic samples showed acceptable accuracy. Fabrication reproducibility of the sensor was good and stability was acceptable. The sensor is a promising new tool for pesticide analysis. ©Springer Science+Business Media B.V. 2007.
语种：
英文
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自由基酰化反应合成4-氯-2，6-二酰基吡啶
期刊：
化学试剂 ,2006年28(1):53-54 ISSN：0258-3283
作者机构：
[张爱东; 汪成进; 涂海洋; 潘克华] 华中师范大学化学学院
关键词：
4-氯吡啶;自由基酰化;4-氯-2,6-二乙酰基吡啶;4-氯-2,6-二丙酰基吡啶
摘要：
由4-氯吡啶通过自由基酰化反应合成4-氯-2，6-二乙酰基吡啶和4-氯-2，6-二丙酰基吡啶，方法简单，产率较文献方法高，分别达到25．7％和51．7％。
语种：
中文
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