Rational design and application of molecularly imprinted sol–gel polymer for the electrochemically selective and sensitive determination of Sudan I
作者：
Chen, Shizhen;Du, Dan* ;Huang, Jing;Zhang, Aiqing;Tu, Haiyang;...
期刊：
Talanta ,2011年84(2):451-456 ISSN：0039-9140
通讯作者：
Du, Dan
作者机构：
[Zhang, Aidong; Du, Dan; Huang, Jing; Tu, Haiyang; Chen, Shizhen] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Zhang, Aiqing] S Cent Univ Nationalities, Minist Educ, Wuhan 430074, Peoples R China.;[Zhang, Aiqing] S Cent Univ Nationalities, Minist Educ, Key Lab Catalysis & Mat Sci State Ethn Affairs Co, Wuhan 430074, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Molecularly imprinted sol-gel;Electrochemical sensor;Gold nanoparticles;Sudan I
摘要：
An electrochemical strategy on the basis of rationally designed molecularly imprinted sol-gel polymer embedded with gold nanoparticles (AuNPs) is developed for the specific and sensitive determination of Sudan I. The rationally designed sensing Sudan I imprinted sol-gel was prepared by mixing Sudan I with 3-aminopropyltriethoxysilane, tetraethoxysilane, chitosan, and AuNPs, followed by copolymerization and extraction of the template molecules. The hybrid forming membrane was characterized by SEM and FTIR-ATR, and used for the linear sweep voltammetric (LSV) determination of Sudan I in water/ethanol solutions. The LSV responses exhibited high sensitivity and selectivity, as discriminated from Sudan I analogues. Under optimal experimental conditions, LSV peak currents were linearly proportional to the concentrations of Sudan I in the range from 0.1 ×10<sup>-7</sup> to 1.0 ×10<sup>-5</sup> M, with a detection limit of 2.0 ×10<sup>-9</sup> M. The strategy is generally applicable in developing sensitive, selective, and moreover, reusable electrochemical sensors for quantitative determination of electroactive species. ©2011 Elsevier B.V. All rights reserved.
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英文
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Electropolymerized multiwalled carbon nanotubes/polypyrrole fiber for solid-phase microextraction and its applications in the determination of pyrethroids
作者：
Chen, Liangbi;Chen, Wenfeng;Ma, Chunhua;Du, Dan* ;Chen, Xi
期刊：
Talanta ,2011年84(1):104-108 ISSN：0039-9140
通讯作者：
Du, Dan
作者机构：
[Chen, Liangbi; Ma, Chunhua] Wuyi Univ, Dept Chem & Environm Engn, Wuyishan 354300, Fujian, Peoples R China.;[Du, Dan] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Chen, Xi; Chen, Wenfeng] Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China.;[Chen, Wenfeng] State Ocean Adm, Inst Oceanog 3, Xiamen, Fujian, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
关键词：
Gas chromatography;Multiwalled carbon nanotubes;Polypyrrole;Pyrethroids;Solid-phase microextraction
摘要：
A novel solid-phase microextraction (SPME) fiber coated with multiwalled carbon nanotubes/polypyrrole (MWCNTs/Ppy) was prepared with an electrochemical method and used for the extraction of pyrethroids in natural water samples. The results showed that the MWCNTs/Ppy coated fiber had high organic stability, and remarkable acid and alkali resistance. In addition, the MWCNTs/Ppy coated fiber was more effective and superior to commercial PDMS and PDMS/DVD fibers in extracting pyrethroids in natural water samples. Under optimized conditions, the calibration curves were found to be linear from 0.001 to 10 μg mL <sup>-1</sup> for five of the six pyrethroids studied, the exception being fenvalerate (which was from 0.005 to 10 μg mL<sup>-1</sup>), and detection limits were within the range 0.12-0.43 ng mL<sup>-1</sup>. The recoveries of the pyrethroids spiked in water samples at 10 ng mL<sup>-1</sup> ranged from 83 to 112%. ©2011 Elsevier B.V. All rights reserved.
语种：
英文
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Self assembly of acetylcholinesterase on a gold nanoparticles-graphene nanosheet hybrid for organophosphate pesticide detection using polyelectrolyte as a linker
作者：
Wang, Ying;Zhang, Sheng;Du, Dan;Shao, Yuyan;Li, Zhaohui;...
期刊：
Journal of Materials Chemistry ,2011年21(14):5319-5325 ISSN：0959-9428
通讯作者：
Li, Jinghong
作者机构：
[Li, Jinghong; Wang, Ying] Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China.;[Shao, Yuyan; Du, Dan; Wang, Ying; Zhang, Sheng; Lin, Yuehe; Wang, Jun; Engelhard, Mark H.; Li, Zhaohui] Pacific NW Natl Lab, Richland, WA 99352 USA.;[Du, Dan] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Li, Jinghong] T;Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China.
摘要：
A nanohybrid of gold nanoparticles (Au NPs) and chemically reduced graphene oxide nanosheets (cr-Gs) was synthesized by in situ growth of Au NPs on the surface of graphene nanosheets in the presence of poly(diallyldimethylammonium chloride) (PDDA), which not only improved the dispersion of Au NPs but also stabilized cholinesterase with high activity and loading efficiency. The obtained nanohybrid was characterized by TEM, XRD, XPS, and electrochemistry. Then an enzyme nanoassembly (AChE/Au NPs/cr-Gs) was prepared by self-assembling acetylcholinesterase (AChE) on Au NP/cr-Gs nanohybrid. An electrochemical sensor based on AChE/Au NPs/cr-Gs was further developed for ultrasensitive detection of organophosphate pesticide. The results demonstrate that the developed approach provides a promising strategy to improve the sensitivity and enzyme activity of electrochemical biosensors.
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英文
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Electrochemical Biosensor Based on Graphene and Carbon nanotubes
作者：
Dan Du
作者机构：
[Dan Du] Key Laboratory of Pesticide and Chemical Biology of Ministry of Education,College of Chemistry,Central China Normal University
会议名称：
The 1st International Congress on Advanced Materials 2011
会议时间：
2011-05-13
会议地点：
中国山东济南
摘要：
Nanomaterials, particularly carbon nanomaterials, have a significant role to play in new developments in each of the biosensor size domains.They have shown great promise in many applications, such as
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英文
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Development of a specific enzyme-linked immunosorbent assay (ELISA) for the analysis of the organophosphorous pesticide fenthion in real samples based on monoclonal antibody
作者：
Wang, Limin;Zhang, Qi;Chen, Defeng;Liu, Yang;Li, Chuanyong;...
期刊：
Analytical Letters ,2011年44(9):1591-1601 ISSN：0003-2719
通讯作者：
Liu, Fengquan
作者机构：
[Zhang, Qi; Li, Chuanyong; Wang, Limin; Liu, Fengquan; Chen, Defeng; Liu, Yang; Hu, Baishi] Nanjing Agr Univ, Minist Agr, Coll Plant Protect, Dept Plant Pathol,Key Lab Monitoring & Management, Nanjing 210095, Peoples R China.;[Du, Dan] Cent China Normal Univ, Minist Educ, Coll Chem, Key Lab Pesticide & Chem, Wuhan, Peoples R China.
通讯机构：
[Liu, Fengquan] N;Nanjing Agr Univ, Minist Agr, Coll Plant Protect, Dept Plant Pathol,Key Lab Monitoring & Management, Nanjing 210095, Peoples R China.
关键词：
Antibody/coating hapten combination;Cross reactivity;Elisa;Monoclonal antibody
摘要：
A new specific monoclonal antibody against the organophosphorous pesticide fenthion was produced based on our previous study. A sensitive and specific Enzyme-Linked Immunosorbent Assay (ELISA) for fenthion based on the new immunizing/coating hapten combination was developed. In this study, the H1 which attempts to expose the aromatic ring group was conjugated with bovine serum albumin (BSA) for the immunogen. All of the haptens that have been synthesized were conjugated with ovalbumin (OVA) for the coating antigen. The efficient antibody/coating conjugated combinations were selected, and the optimized ELISA was developed. In the optimized system, the IC50 value was 5.8 ng mL-1 with the detection limit (IC20) of 0.028 ng mL-1. The cross reactivity with all of the tested pesticides was lower than the 0.5%. Also, the system can detect the fenthion addition to the real samples such as soil, water, rice, and Chinese cabbages. © Taylor & Francis Group, LLC.
语种：
英文
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Nanoparticle-based immunosensor with apoferritin templated metallic phosphate label for quantification of phosphorylated acetylcholinesterase
作者：
Du, Dan
* ;Chen, Aiqiong;Xie, Yunying;Zhang, Aidong
（ ;Lin, Yuehe
期刊：
Biosensors and Bioelectronics ,2011年26(9):3857-3863 ISSN：0956-5663
通讯作者：
Du, Dan
作者机构：
[Zhang, Aidong; Chen, Aiqiong; Du, Dan; Xie, Yunying] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.;[Lin, Yuehe] Pacific NW Natl Lab, Richland, WA 99352 USA.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Amplification;Apoferritin;Biomarker;Immunosensor;Organophosphate pesticides
摘要：
A new sandwich-like electrochemical immunosensor has been developed for quantification of organophosphorylated acetylcholinesterase (OP-AChE), an exposure biomarker of organophosphate pesticides and nerve agents. Zirconia nanoparticles (ZrO2 NPs) were anchored on a screen printed electrode (SPE) to preferably capture OP-AChE adducts by metal chelation with phospho-moieties, which was selectively recognized by lead phosphate-apoferritin labeled anti-AChE antibody (LPA-anti-AChE). The sandwich-like immunoreactions were performed among ZrO2 NPs, OP-AChE and LPA-anti-AChE to form ZrO2/OP-AChE/LPA-anti-AChE complex and the released lead ions were detected on a disposable SPE. The binding affinity was investigated by both square wave voltammetry (SWV) and quartz crystal microbalance (QCM) measurements. The proposed immunosensor yielded a linear response current over a broad OP-AChE concentrations range from 0.05nM to 10nM, with detection limit of 0.02nM, which has enough sensitivity for monitoring of low-dose exposure to OPs. This method avoids the drawback of unavailability of commercial OP-specific antibody as well as amplifies detection signal by using apoferritin encoded metallic phosphate nanoparticle tags. This nanoparticle-based immunosensor offers a new method for rapid, sensitive, selective and inexpensive quantification of phosphorylated adducts for monitoring of OP pesticides and nerve agents exposures. © 2011 Elsevier B.V.
语种：
英文
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Graphene-based immunosensor for electrochemical quantification of phosphorylated p53 (S15)
作者：
Xie, Yunying;Chen, Aigiong;Du, Dan* ;Lin, Yuehe
期刊：
Analytica Chimica Acta ,2011年699(1):44-48 ISSN：0003-2670
通讯作者：
Du, Dan
作者机构：
[Du, Dan; Chen, Aigiong; Xie, Yunying] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Lin, Yuehe] Pacific NW Natl Lab, Richland, WA 99352 USA.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Electrochemistry;Graphene;Immunosensor;Phosphorylated p53
摘要：
We reported a graphene-based immunosensor for electrochemical quantification of phosphorylated p53 on serine 15 (phospho-p5315), a potential biomarker of gamma-radiation exposure. The principle is based on sandwich immunoassay and the resulting immunocomplex is formed among phospho-p53 capture antibody, phospho-p5315 antigen, biotinylated phospho-p5315 detection antibody and horseradish peroxidase (HRP)-labeled streptavidin. The introduced HRP results in an electrocatalytic response to reduction of hydrogen peroxide in the presence of thionine. Graphene served as sensor platform not only promotes electron transfer, but also increases the surface area to introduce a large amount of capture antibody, thus increasing the detection sensitivity. The experimental conditions including blocking agent, immunoreaction time and substrate concentration have been optimized. Under the optimum conditions, the increase of response current is proportional to the phospho-p5315 concentration in the range of 0.2-10ngmL-1, with the detection limit of 0.1ngmL-1. The developed immunosensor exhibits acceptable stability and reproducibility and the assay results for phospho-p5315 are in good correlation with the known values. This easily fabricated immunosensor provides a new promising tool for analysis of phospho-p5315 and other phosphorylated proteins. © 2011 Elsevier B.V.
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英文
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Magnetic electrochemical sensing platform for biomonitoring of exposure to organophosphorus pesticides and nerve agents based on simultaneous measurement of total enzyme amount and enzyme activity
作者：
Du, Dan;Wang, Jun* ;Wang, Limin;Lu, Donglai;Smith, Jordan N.;...
期刊：
Analytical Chemistry ,2011年83(10):3770-3777 ISSN：0003-2700
通讯作者：
Wang, Jun
作者机构：
[Smith, Jordan N.; Wang, Limin; Timchalk, Charles; Du, Dan; Lin, Yuehe; Wang, Jun; Lu, Donglai] Pacific NW Natl Lab, Richland, WA 99352 USA.;[Du, Dan] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, Jun] P;Pacific NW Natl Lab, Richland, WA 99352 USA.
摘要：
We report a new approach for electrochemical quantification of enzymatic inhibition and phosphorylation for biomonitoring of exposure to organophosphorus (OP) pesticides and nerve agents based on a magnetic bead (MB) immunosensing platform. The principle of this approach is based on the combination of MB immunocapture-based enzyme activity assay and competitive immunoassay of the total amount of enzyme for simultaneous detection of enzyme inhibition and phosphorylation in biological fluids. Butyrylcholinesterase (BChE) was chosen as a model enzyme. In competitive immunoassay, the target BChE in a sample competes with the BChE immobilized on the MBs to bind to the limited sites of anti-BChE antibody labeled with quantum dots (QD-anti-BChE), followed by stripping voltammetric analysis of the bound QD conjugate on the MBs. This assay shows a linear response over the total BChE concentration range of 0.1-20 nM. Simultaneous real time BChE activity was measured on an electrochemical carbon nanotube-based sensor coupled with a microflow injection system after immunocapture by the MB-anti-BChE conjugate. Therefore, the formed phosphorylated BChE adduct (OP-BChE) can be estimated by the difference values of the total amount of BChE (including active and OP-inhibited) and active BChE from established calibration curves. This approach not only eliminates the difficulty in screening of low-dose OP exposure (less than 20% inhibition of BChE) because of individual variation of BChE values but also avoids the drawback of the scarce availability of OP-BChE antibody. It is sensitive enough to detect 0.5 nM OP-BChE, which is less than 2% BChE inhibition. This method offers a new method for rapid, accurate, selective, and inexpensive quantification of OP-BChE and enzyme inhibition for biomonitoring of OP and nerve agent exposures. © 2011 American Chemical Society.
语种：
英文
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Detection of organophosphate pesticide using polyaniline and carbon nanotubes composite based on acetylcholinesterase inhibition
作者：
Chen, Daiqin;Chen, Chuanhuizi;Du, Dan*
期刊：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY ,2010年10(9):5662-5666 ISSN：1533-4880
通讯作者：
Du, Dan
作者机构：
[Du, Dan; Chen, Daiqin; Chen, Chuanhuizi] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;PANI-MWCNTs Composite Film;Malathion
摘要：
Acetylcholinesterase (AChE) activity may be useful biomarker for detecting of organophosphate pesticides (OP). Thus a sensitive biosensor for quantitative determination of OP based on AChE biomonitoring was developed. Multi-walled carbon nanotube (MWCNT)/polyaniline (PANI) composite film was prepared by electrochemical polymerization. The immobilized AChE catalyzed the hydrolysis of acetylthiocholine chloride to produce thiocholine, which engendered an irreversible oxidation peak. The enzyme activity was monitored by measuring the oxidation current of thiocholine and further detection of OP. The developed sensor provided a new promising tool for pesticide analysis and assay of enzyme activity.
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英文
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碳纳米管修饰的玻碳电极测定蕃茄制品中苏丹红Ⅰ
作者：
张剑德;杜丹
期刊：
分析科学学报 ,2010年26(5):617-620 ISSN：1006-6144
作者机构：
[张剑德] 湖北职业技术学院;[杜丹] 华中师范大学化学学院
关键词：
玻碳电极;苏丹红Ⅰ;循环伏安法;线性扫描法
摘要：
利用吸附法将苏丹红Ⅰ固定在碳纳米管修饰的玻碳电极(CNT)表面,制成稳定的固载苏丹红Ⅰ碳纳米管修饰电极,再利用循环伏安法(CV),线性扫描伏安法(LSV)研究了苏丹红Ⅰ在CNT上的电化学行为.在pH=2.0的乙醇-B-R缓冲溶液中,苏丹红Ⅰ在-0.16V附近产生一灵敏的峰.在优化的最佳实验条件下,溶液中苏丹红Ⅰ峰电流iP与其浓度在8.0×10-7至1.6×10-5mol/L范围内有良好的线性关系,回归方程为iP=2.939+9.963c(×10-6mol/L),R=0.9963.检出限为9.0×10-8mol/L.CNT电极无毒,无污染,灵敏度高,为检测苏丹红Ⅰ提供了一种安全有效的新方法
语种：
中文
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Sensitive immunosensor for cancer biomarker based on dual signal amplification strategy of graphene sheets and multienzyme functionalized carbon nanospheres
作者：
Du, Dan;Zou, Zhexiang;Shin, Yongsoon;Wang, Jun;Wu, Hong;...
期刊：
Analytical Chemistry ,2010年82(7):2989-2995 ISSN：0003-2700
通讯作者：
Lin, Yuehe
作者机构：
[Du, Dan; Zou, Zhexiang; Wu, Hong; Lin, Yuehe; Shin, Yongsoon; Wang, Jun; Engelhard, Mark H.; Liu, Jun] Pacific NW Natl Lab, Richland, WA 99352 USA.;[Aksay, Ilhan A.] Princeton Univ, Dept Chem Engn, Princeton, NJ 08544 USA.;[Du, Dan] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Lin, Yuehe] P;Pacific NW Natl Lab, Richland, WA 99352 USA.
摘要：
A novel electrochemical immunosensor for sensitive detection of cancer biomarker α-fetoprotein (AFP) is described that uses a graphene sheet sensor platform and functionalized carbon nanospheres (CNSs) labeled with horseradish peroxidase-secondary antibodies (HRP-Ab2). Greatly enhanced sensitivity for the cancer biomarker is based on a dual signal amplification strategy: first, the synthesized CNSs yielded a homogeneous and narrow size distribution, which allowed several binding events of HRP-Ab2 on each nanosphere. Enhanced sensitivity was achieved by introducing the multibioconjugates of HRP-Ab2-CNSs onto the electrode surface through "sandwich" immunoreactions. Second, functionalized graphene sheets used for the biosensor platform increased the surface area to capture a large amount of primary antibodies (Ab1), thus amplifying the detection response. On the basis of the dual signal amplification strategy of graphene sheets and the multienzyme labeling, the developed immunosen-sor showed a 7-fold increase in detection signal compared to the immunosensor without graphene modification and CNSs labeling. The proposed method could respond to 0.02 ng mL-1 AFP with a linear calibration range from 0.05 to 6 ng mL-1. This amplification strategy is a promising platform for clinical screening of cancer biomarkers and point-of-care diagnostics. © 2010 American Chemical Society.
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英文
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One-step synthesis of multiwalled carbon nanotubes-gold nanocomposites for fabricating amperometric acetylcholinesterase biosensor
作者：
Du, Dan
* ;Wang, Minghui;Cai, Jie;Qin, Yuehua;Zhang, Aidong
（ 期刊：
Sensors and Actuators B-Chemical ,2010年143(2):524-529 ISSN：0925-4005
通讯作者：
Du, Dan
作者机构：
[Zhang, Aidong; Du, Dan; Qin, Yuehua; Wang, Minghui] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Cai, Jie] Wuhan Iron & Steel Co, Ctr Technol, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Multiwalled carbon nanotubes;Nanocomposites;Acetylcholinesterase;Malathion;Biosensoer
摘要：
A facile, one-step synthesis of nanocomposites using multiwalled carbon nanotube coating gold nanoparticles (MWCNTs-Au) was presented. Scanning electron microscopy and UV-vis spectroscopy confirmed that more than 97% of gold nanoparticles have been loaded on the surface of carbon nanotubes without congregation. The formed MWCNTs-Au nanocomposites offered an extremely hydrophilic surface for biomolecule adhesion, leading to a stable acetylcholinesterase (AChE) biosensor. Due to the excellent conductivity of the nanocomposites, the immobilized AChE showed favorable affinity to acetylthiocholine (ATCl) and could catalyze the hydrolysis of ATCl with a K<inf>m</inf><sup>app</sup> value of 268 μM to form thiocholine, which was then oxidized to produce a detectable and fast response. Based on the inhibition of organophosphates (OPs) on the enzymatic activity of AChE, the magnitude of peak current from thiocholine on the biosensor is a simple and effective way to biomonitoring of OPs exposure. Using malathion as a model compound, the inhibition of malathion was proportional to its concentration ranging from 1.0 to 1000 ng mL<sup>-1</sup> and from 2 to 15 μg mL<sup>-1</sup>, with a detection limit 0.6 ng mL<sup>-1</sup>. The developed biosensor exhibited good reproducibility and acceptable stability, thus providing a new promising tool for analysis of enzyme inhibitors. ©2009 Elsevier B.V. All rights reserved.
语种：
英文
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Acetylcholinesterase biosensor design based on carbon nanotube-encapsulated polypyrrole and polyaniline copolymer for amperometric detection of organophosphates
作者：
Du, Dan
* ;Ye, Xiaoxue;Cai, Jie;Liu, Juan;Zhang, Aidong
（ 期刊：
Biosensors and Bioelectronics ,2010年25(11):2503-2508 ISSN：0956-5663
通讯作者：
Du, Dan
作者机构：
[Zhang, Aidong; Liu, Juan; Du, Dan; Ye, Xiaoxue] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Cai, Jie] Wuhan Iron & Steel Co, Ctr Technol, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Biosensor;Carbon nanotubes;Organophosphates;Polypyrrole-polyaniline copolymer
摘要：
A simple method to immobilize acetylcholinesterase (AChE) on polypyrrole (PPy) and polyaniline (PANI) copolymer doped with multi-walled carbon nanotubes (MWCNTs) was proposed. The synthesized PAn-PPy-MWCNTs copolymer presented a porous and homogeneous morphology which provided an ideal size to entrap enzyme molecules. Due to the biocompatible microenvironment provided by the copolymer network, the obtained composite was devised for AChE attachment, resulting in a stable AChE biosensor for screening of organophosphates (OPs) exposure. MWCNTs promoted electron-transfer reactions at a lower potential and catalyzed the electro-oxidation of thiocholine, thus increasing detection sensitivity. Based on the inhibition of OPs on the AChE activity, using malathion as a model compound, the inhibition of malathion was proportional to its concentration ranging from 0.01 to 0.5. and mu;g/mL and from 1 to 25. and mu;g/mL, with a detection limit of 1.0. ng/mL. The developed biosensor exhibited good reproducibility and acceptable stability, thus providing a new promising tool for analysis of enzyme inhibitors. © 2010 Elsevier B.V.
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英文
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One-Step Electrochemically Deposited Gold Nanoparticles Interface Grafted with Avidin for Acetylcholinesterase Biosensor Design
作者：
Zhang, Weiying;Ding, Jiawang;Qin, Yuehua;Liu, Deli* ;Du, Dan
期刊：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY ,2010年10(9):5685-5691 ISSN：1533-4880
通讯作者：
Liu, Deli
作者机构：
[Zhang, Weiying; Liu, Deli; Du, Dan; Qin, Yuehua; Ding, Jiawang] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Liu, Deli] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
关键词：
Electrodeposition;Gold Nanoparticles;Biotin Labeled Acetylcholinesterase;Dimethoate;Biosensor
摘要：
In this study, an interface embedded in situ gold nanoparticles (AuNPs) and biotin in chitosan hydro-gel was constructed by one-step electrochemical deposition in solution containing tetrachloroauric (III) acid, biotin and chitosan. This deposited interface acts as biosensing platforms and provides specific binding sites for avidin, which are further capable of attaching any biotinylated bimolec-ular for biosensor design. Atomic force microscopy (AFM), A.C. impedance and surface plasmon resonance were used to characterize this interface. The immobilized acetylcholinesterase (AChE), as a model, showed excellent activity to its substrate and provided a quantitative measurement of organophosphate pesticide. Under the optimal experimental conditions, the inhibition of dimethoate was proportional to its concentrations in the range of 0.05 to 15 μg mL<sup>-1</sup> with detection limit of 0.001 μg mL <sup>-1</sup>. The simple method showed good fabrication reproducibility and acceptable stability, which provided a new avenue for electrochemical biosensor design. ©2010 American.
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英文
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Covalent coupling of organophosphorus hydrolase loaded quantum dots to carbon nanotube/Au nanocomposite for enhanced detection of methyl parathion
作者：
Du, Dan* ;Chen, Wenjuan;Zhang, Weiying;Liu, Deli;Li, Haibing;...
期刊：
Biosensors and Bioelectronics ,2010年25(6):1370-1375 ISSN：0956-5663
通讯作者：
Du, Dan
作者机构：
[Zhang, Weiying; Liu, Deli; Du, Dan; Li, Haibing; Chen, Wenjuan] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Du, Dan; Lin, Yuehe] Pacific NW Natl Lab, Richland, WA 99352 USA.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Amplification;CdTe quantum dots;Methyl parathion;Methyl parathion degrading enzyme;Multiwalled carbon nanotube
摘要：
An amperometric biosensor for highly selective and sensitive determination of methyl parathion (MP) was developed based on dual-signal amplification: (1) a large amount of introduced enzyme on the electrode surface and (2) synergistic effects of nanoparticles towards enzymatic catalysis. The fabrication process includes (1) electrochemical deposition of gold nanoparticles by a multi-potential step technique at multiwalled carbon nanotube (MWCNT) film pre-cast on a glassy carbon electrode and (2) immobilization of methyl parathion degrading enzyme (MPDE) onto a modified electrode through CdTe quantum dots (CdTe QDs) covalent attachment. The introduced MWCNT and gold nanoparticles significantly increased the surface area and exhibited synergistic effects towards enzymatic catalysis. CdTe QDs are further used as carriers to load a large amount of enzyme. As a result of these two important enhancement factors, the proposed biosensor exhibited extremely sensitive, perfectly selective, and rapid response to methyl parathion in the absence of a mediator. The detection limit was 1.0 ng/mL. Moreover, since MPDE hydrolyzes pesticides containing the P-S bond, it showed high selectivity for detecting MP and many interfering compounds, such as carbamate pesticides. Other organophosphorous pesticides and oxygen-containing inorganic ions (SO42-, NO3-) did not interfere with the determination. The proposed MPDE biosensor presents good reproducibility and stability, and the MPDE is not poisoned by organophosphate pesticides. Unlike cholinesterase-based biosensor, the MPDE biosensor can be potentially reused and is suitable for continuous monitoring. © 2009 Elsevier B.V. All rights reserved.
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One-step electrochemical deposition of Prussian Blue-multiwalled carbon nanotube nanocomposite thin-film: preparation, characterization and evaluation for H2O2 sensing
作者：
Du, Dan* ;Wang, Minghui;Qin, Yuehua;Lin, Yuehe
期刊：
Journal of Materials Chemistry ,2010年20(8):1532-1537 ISSN：0959-9428
通讯作者：
Du, Dan
作者机构：
[Du, Dan; Qin, Yuehua; Wang, Minghui] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Du, Dan; Lin, Yuehe] Pacific NW Natl Lab, Richland, WA 99352 USA.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
摘要：
1.IntroductionCarbon nanotubes (CNTs) with single and multiple walls have attracted considerable interest since their discovery in 19911because of their unique electronic properties, high chemical stability, and extremely high mechanical strength and stiffness.2–4These advantages suggest that CNTs have a significant potential application in nanodevices, particularly field-effect transistors (FETs),5,6nanoprobes,7bioelectronics, and sensors.8–11It was reported that CNT-modified electrodes have been widely used in the catalytic electrochemistry of some interesting biomolecules, such as ascorbic acid,12cytochrome c,13β-nicotinamide adenine dinucleotide (NADH),14dopamine,15adenine,16guanine,16and especially hydrogen peroxide (H2O2).17The successful application of CNT-based composite materials usually requires chemical modification of the CNTs.18Modifications of the CNTs with polymers or biomolecules are usually used to improve their dispersion and orientation in aqueous solutions.19,20Potassium iron(iii) hexacyanoferrate(ii) or Prussian Blue (PB) is an inorganic polycrystal with well known electrochromic and electrocatalytical properties.21Because of its high activity and selectivity toward the reduction of hydrogen peroxide and oxygen, PB is usually considered as an “artificial enzyme peroxidase”22and has been extensively used in constructing electrochemical biosensors.23–26Many methods have been used to prepare PB-modified electrodes. Recent reports indicate that PB can be photochemically or electrochemically deposited on electrode surfaces from an acidic ferricyanide solution.27,28The combination of PB and CNTs has received more attention,29–31but in most studies, PB particles were pre-synthesized and then mixed with CNTs.32There are only very few papers reporting the synthesis of PB crystallites on CNTs matrix by electrochemical co-deposition method.H2O2, which is a product of enzymatic reactions, is a product of industry and atomic power stations. It is a chemical threat to the environment and is recognized as one of the major factors in the progression of important diseases. However, the problem of H2O2amperometric detection is well known to all the researchers involved in the biosensor field since H2O2is the side product of a classic reaction catalyzed by oxidase enzymes,33The concentration of H2O2is directly proportional to the concentration of the enzymatic substrate. The capability of PB to reduce H2O2has attracted the attention of the biosensor community.In the present study, PB crystallites were synthesized on the surface of multiwalled carbon nanotubes (MWCNTs) with an one-step electrochemical co-deposition method to fabricate a PB–MWCNT/Au electrode without binders (Scheme 1). The MWCNTs not only act as a carrier of PB, but also as a modifier with a catalytic function. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy revealed that PB-coated MWCNTs in these films facilitated improved electron and ion transfer relative to pure PB films. They also exhibited larger electrode-specific capacitance than PB alone. Compared with a PB film, the PB–MWCNT composite film showed a larger response current to the reduction of H2O2due to the synergistic effects between the MWCNTs and PB particles. The method presented here demonstrates the potential of using CNTs and other inorganic or metal nanoparticles to construct bioelectronic devices and biosensors.Schematic representation of PB–MWCNT/Au nanocomposite and the electrocatalytic mechanism for H2O2reduction.
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Sensitive acetylcholinesterase biosensor based on assembly of β-cyclodextrins onto multiwall carbon nanotubes for detection of organophosphates pesticide
作者：
Du, Dan
* ;Wang, Minghui;Cai, Jie;Zhang, Aidong
（ 期刊：
Sensors and Actuators B-Chemical ,2010年146(1):337-341 ISSN：0925-4005
通讯作者：
Du, Dan
作者机构：
[Zhang, Aidong; Du, Dan; Wang, Minghui] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei Province, Peoples R China.;[Cai, Jie] Technol Ctr Wuhan Iron & Steel Co, Wuhan 430080, Peoples R China.;[Du, Dan] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Luoyu Rd 152, Wuhan 430079, Hubei Province, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Luoyu Rd 152, Wuhan 430079, Hubei Province, Peoples R China.
关键词：
MWCNTs-beta-CD composite;Acetylcholinesterase;Dimethoate;Biosensor
摘要：
This work described a highly sensitive amperometric biosensor for organophosphates (OPs) pesticides based on immobilization of acetylcholinesterase (AChE) on multiwall carbon nanotubes (MWCNTs)-β-cyclodextrin (β-CD) composite modified glassy carbon electrode. The synthesized composite through polymer wrapping strategy has been characterized by attenuated total reflection Fourier-transform infrared spectra (ATR-FTIR) measurement and scanning electron microscopy (SEM) images. Due to the good dispersibility and porous structures of MWCNTs-β-CD composite, the resulting surface provided a favorable microenvironment for acetylcholinesterase biosensor fabrication and maintained the bioactivity of AChE for screening of OPs exposure. MWCNTs promoted electron-transfer reactions at a lower potential and catalyzed the electro-oxidation of thiocholine, thus increasing detection sensitivity. Based on the inhibition of OPs on the AChE activity, using dimethoate as a model compound, the inhibition of dimethoate was proportional to its concentration ranging from 0.01 to 2.44 and 2.44 to 10.00 μM, with a detection limit was 2 nM (S/N = 3). The developed biosensor exhibited good reproducibility and acceptable stability, thus providing a new promising tool for analysis of enzyme inhibitors. ©2010 Elsevier B.V. All rights reserved.
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Quantum dot-based immunochromatographic fluorescent biosensor for biomonitoring trichloropyridinol, a biomarker of exposure to chlorpyrifos
作者：
Zou, Zhexiang;Du, Dan;Wang, Jun* ;Smith, Jordan N.;Timchalk, Charles;...
期刊：
Analytical Chemistry ,2010年82(12):5125-5133 ISSN：0003-2700
通讯作者：
Wang, Jun
作者机构：
[Smith, Jordan N.; Timchalk, Charles; Du, Dan; Zou, Zhexiang; Lin, Yuehe; Wang, Jun] Pacific NW Natl Lab, Richland, WA 99352 USA.;[Li, Yaoqun; Zou, Zhexiang] Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China.;[Li, Yaoqun; Zou, Zhexiang] Xiamen Univ, Coll Chem & Chem Engn, Key Lab Analyt Sci, Xiamen 361005, Peoples R China.;[Du, Dan] Cent China Normal Univ, Coll Chem, Wuhan 430039, Peoples R China.
通讯机构：
[Wang, Jun] P;Pacific NW Natl Lab, Richland, WA 99352 USA.
摘要：
A novel and portable fluorescent sensor that integrates an immunochromatographic test strip assay (ITSA) with a quantum dot (QD) label and a test strip reader was described in this study for simple, rapid, and sensitive biomonitoring of an organophosphorus pesticide metabolite. The principle of this sensor is based on a competitive immunoreaction that was performed on an immunochromatographic test strip, where analytes compete with competitors (QD-conjugated analogs) to bind to antibodies on a test zone. Captured QDs serve as signal vehicles for fluorescent readout. In this work, 3,5,6- trichloropyridinol (TCP) is used as a model analyte to demonstrate the performance of the immunosensor. QD-TCP conjugates were synthesized and characterized with X-ray photoelectron spectroscopy (XPS) and fluorescence spectroscopy. Some parameters (e.g., the amount of QD-modified TCP and immunoreaction time) that govern sensitivity and reproducibility of ITSA were optimized. Under optimal conditions, the sensor has a wide dynamic range and is capable of detecting a minimum 1.0 ng/mL TCP standard analyte in 15 min. The sensor has been successfully applied for detection of TCP spiked in rat plasma with average recovery of 102.0%. Results demonstrate that this sensor provides a rapid, clinically accurate, and quantitative tool for TCP detection and shows great promise for in-field and point-of-care (POC) quantitative testing and screening for metabolite biomarkers, e.g., TCP, for humans exposed to pesticides. © 2010 American Chemical Society.
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Rhodamine-based ratiometric fluorescence sensing for the detection of mercury(II) in aqueous solution
作者：
Liu, Haizhu;Yu, Ping;Du, Dan;He, Chunyan;Qiu, Bin;...
期刊：
Talanta ,2010年81(1-2):433-437 ISSN：0039-9140
通讯作者：
Chen, Xi
作者机构：
[Chen, Xi; He, Chunyan; Qiu, Bin; Liu, Haizhu; Chen, Guonan; Yu, Ping] Fuzhou Univ, Dept Chem, Key Lab Anal & Detect Technol Food Safety, Minist Educ, Fuzhou 350002, Peoples R China.;[Chen, Xi] Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China.;[Du, Dan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Chen, Xi] X;Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China.
关键词：
Silica nanoparticles;Mercury ion;Rhodamine 6G;Ratiometric fluorescence
摘要：
Novel ratiometric fluorescent silica nanoparticles with high selectivity towards Hg<sup>2+</sup> were synthesized for the detection of Hg<sup>2+</sup>. Hg<sup>2+</sup> promoted the ring opening of spirolactam in the rhodamine moiety grafted onto the silica nanoparticles, resulting in a change in the fluorescence intensity. The fluorescence intensity was proportional to the Hg<sup>2+</sup> concentration, and the detection limit (S/N = 3) for Hg<sup>2+</sup> was found to be 2.59 ×10<sup>-9</sup> mol L<sup>-1</sup> with a linear range from 0.4 to 8 ×10<sup>-7</sup> mol L<sup>-1</sup>. In addition, the morphology of the silica nanoparticles, the effects of pH and co-existing substances, and the reversibility were investigated. The proposed approach was successfully applied to the determination of Hg<sup>2+</sup> in water samples. ©2010 Elsevier B.V. All rights reserved.
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