基于硫代胆碱调控金纳米粒子生长的电化学传感器
作者机构：
[张爱东; 丁家旺; 杜丹] 农药与化学生物学教育部重点实验室,华中师范大学
会议名称：
中国化学会第十四届有机分析及生物分析学术研讨会
会议时间：
2007-10-01
会议地点：
武汉
会议论文集名称：
中国化学会第十四届有机分析及生物分析学术研讨会论文集
关键词：
硫代胆碱;金纳米粒子;电化学传感器;纳米生物;多巴胺;电子传导;农药测定
摘要：
<正>利用生物催化合成金属纳米粒子,调控金属纳米粒子的生长已用于纳米生物科技领域。进来研究显示:硫代胆碱,过氧化氢,烟碱腺嘌呤二核苷酸(磷酸盐),葡萄糖与葡萄糖氧化酶,以及一些活性小分子可以作为还原剂,在纳米金种存在的情况下,促使纳米金的长大。而且一系列的神经传递素如多巴胺,肾上腺素等在无纳米金种存在的情况下,亦可以调控纳米金的生成和长大。基于不同粒径的纳米金表现出不同的紫外吸收, 构建了光学传感
语种：
中文
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Comparison of pesticide sensitivity by electrochemical test based on acetylcholinesterase biosensor
作者：
Du, Dan
* ;Huang, Xi;Cai, He;Zhang, Aidong
（ 期刊：
Biosensors and Bioelectronics ,2007年23(2):285-289 ISSN：0956-5663
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minist Educ, Key Lab Pest & Chem Biol, Wuhan 430079, Peoples R China.;Wuhan Iron & Steel Co, Ctr Technol, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pest & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Biosensor;Electrochemical test;Immobilization;Pesticide sensitivity
摘要：
Based on the change in electrochemical behavior of enzymatic activity induced by pesticide, a novel electrochemical method has been devised for investigation of pesticide sensitivity using acetylcholinesterase (AChE) biosensor. Because of the excellent biocompatibility and good stability of chitosan matrix, it prevented leakage of the AChE from electrode. Multiwall carbon nanotube (MWNT) promoted electron transfer reaction at a lower potential and catalyzed the electro-oxidation of thiocholine, thus amplifying the sensitivity and amperometric response of the biosensor. Four pesticides of carbaryl, malathion, dimethoate and monocrotophos were selected to discuss their inhibition efficiencies to AChE. The inhibition curves were similar to Michealis-Menten and the Michealis-Menten constants (Km) were calculated to be 0.96 μM, 1.78 μM, 1.97 μM and 4.28 μM, respectively. Ninety-five percent reactivation of the inhibited AChE could be regenerated using pralidoxime iodide within 8 min. The proposed electrochemical pesticide sensitivity test exhibited high sensitivity, low cost and simplified procedures, which is a promising new tool for comparison of pesticide sensitivity and for selection of the most efficient enzyme inhibitors. © 2007 Elsevier B.V. All rights reserved.
语种：
英文
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One-step electrochemically deposited interface of chitosan–gold nanoparticles for acetylcholinesterase biosensor design
作者：
Du, Dan
* ;Ding, Jiawang;Cai, Jie;Zhang, Aidong
（ 期刊：
Journal of Electroanalytical Chemistry ,2007年605(1):53-60 ISSN：1572-6657
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Wuhan Iron & Steel Co, Ctr Technol, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Biosensor;Chitosan;Electrodeposition;Gold nanoparticles;Organophosphate pesticides
摘要：
A novel interface embedded in situ gold nanoparticles (GNPs) in chitosan hydrogel was constructed by one-step electrochemical deposition in solution containing tetrachloroauric (III) acid and chitosan. This deposited interface possessed excellent biocompatibility and good stability by characterization of attenuated total reflection Fourier-transform infrared spectra (ATR-FTIR), atomic force microscopy (AFM) and UV-vis spectra. The immobilized AChE, as a model, showed excellent activity to its substrate and provided a quantitative measurement of organophosphate pesticides involved in the inhibition action. Operational parameters, including the deposition time, tetrachloroauric (III) acid concentration have been optimized. Under the optimal electrodeposition, an amperometric sensor for the fast determination of malathion and monocrotophos, respectively was developed with detection limit of 0.001 μg mL-1. The simple method showed good fabrication reproducibility and acceptable stability, which provided a new avenue for electrochemical biosensor design. © 2007 Elsevier B.V. All rights reserved.
语种：
英文
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Reagentless amperometric carbohydrate antigen 19-9 immunosensor based on direct electrochemistry of immobilized horseradish peroxidase
作者：
Du Dan
* ;Xu Xiaoxing;Wang Shengfu;Zhang Aidong
（ 期刊：
Talanta ,2007年71(3):1257-1262 ISSN：0039-9140
通讯作者：
Du Dan
作者机构：
[Du Dan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Changshu Inst Technol, Dept Chem, Changshu 215500, Peoples R China.;Minist Educ, Key Lab Synth & Applicat Organ Funct Mol, Wuhan 430062, Peoples R China.
通讯机构：
[Du Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Carbohydrate antigen 19-9 (CA19-9);Colloidal gold nanoparticles;Electrochemical sensors;Enzyme labeled antibody;Immunosensor
摘要：
A reagentless immunosensor for rapid determination of carbohydrate antigen 19-9 (CA19-9) in human serum was proposed. This strategy was based on the immobilization of antibody in colloidal gold nanoparticle modified carbon paste electrode and the direct electrochemistry of horseradish peroxidase (HRP) that was labeled to a CA19-9 antibody. The nanoparticles were efficient for preserving the activity of immobilized biomolecules. Thus, the immobilized HRP displayed its direct electrochemistry with a rate constant of 1.02 s<sup>-1</sup>. The incubation of the immunosensor in phosphate buffer solution (PBS) including CA19-9 antigen leading to the formation of antigen-antibody complex, which made the block of electron transfer of HRP toward electrode and resulted in significant peak current decrease of HRP. Under the optimal conditions, the current decrease was proportional to CA19-9 concentrations ranging from 2 to 30 U/ml with a detection limit of 1.37 U/ml at a current decrease by 10%. The immunosensor showed an acceptable accuracy compared with those obtained from immunoradiometric assays, with intra-assay coefficient of 7.3 and 6.9% at CA19-9 concentrations of 5 and 15 U/ml, respectively, and inter-assay coefficient of 9.6% at a CA19-9 concentration of 20 U/ml. The storage stability was acceptable in a pH 7.0 PBS at 4 °C for 10 days. This method avoids the addition of electron transfer mediator, thus simplifies the immunoassay procedure and decreases the analytical time. It provides a new promising platform for clinical immunoassay. ©2006 Elsevier B.V. All rights reserved.
语种：
英文
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An amperometric acetylthiocholine sensor based on immobilization of acetylcholinesterase on a multiwall carbon nanotube-cross-linked chitosan composite
作者：
Du, Dan
* ;Huang, Xi;Cai, Jie;Zhang, Aidong
（ ;Ding, Jiawang;...
期刊：
Analytical and Bioanalytical Chemistry ,2007年387(3):1059-1065 ISSN：1618-2642
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minst Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Technol Ctr Wuhan Iron & Steel Co, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minst Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Acetylthiocholine sensor;Chitosan;Composite film;Multiwall carbon nanotube
摘要：
A simple method has been devised for immobilization of acetylcholinesterase (AChE)-covalent bonding to a multiwall carbon nanotube (MWNT)-cross-linked chitosan composite (CMC)-and a sensitive amperometric sensor for rapid detection of acetylthiocholine (ATCl) has been based on this. Fourier-transform infrared spectroscopy proved that the native structure of the immobilized enzyme was preserved on this chemically clean and homogeneous composite film, because of the excellent biocompatibility and non-toxicity of chitosan. Glutaraldehyde was used as cross-linker to covalently bond the AChE, and efficiently prevented leakage of the enzyme from the film. Because of the inherent conductive properties of the MWNT, the immobilized AChE had greater affinity for ATCl and excellent catalytic effect in the hydrolysis of ATCl, with a $$K^{{{\text{app}}}}_{{\text{m}}} $$ value of 132 μmol L-1, forming thiocholine, which was then oxidized to produce a detectable and rapid response. Under optimum conditions the amperometric current increased linearly with the increasing concentration of ATCl in the range 2.0-400 μmol L-1, with a detection limit of 0.10 μmol L-1. Fabrication reproducibility of the sensor was good and the stability was acceptable. The sensor is a promising new tool for characterization of enzyme inhibitors and for pesticide analysis. [Figure not available: see fulltext.]. © Springer-Verlag 2007.
语种：
英文
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Recognition of glycoprotein peroxidase via Con A-carrying self-assembly layer on gold
作者：
Liu, Songqin* ;Wang, Kewei;Du, Dan;Sun, Yueming;He, Lin
期刊：
Biomacromolecules ,2007年8(7):2142-2148 ISSN：1525-7797
通讯作者：
Liu, Songqin
作者机构：
[Liu, Songqin] SE Univ, Coll Chem & Chem Engn, Nanjing 210096, Peoples R China.;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;N Carolina State Univ, Dept Chem, Raleigh, NC 27695 USA.
通讯机构：
[Liu, Songqin] S;SE Univ, Coll Chem & Chem Engn, Nanjing 210096, Peoples R China.
摘要：
We have successfully fabricated a self-assembled layer of concanavalin A (Con A) on a gold surface for recognition of glycoproteins. The type IV Con A is covalently bound to 11-mercaptoundecanoic acid (MUA) on gold with a 2-(5-norbornene-2,3-dicarboximido)-1,1,3,3-tetramethyluronium tetrafluoroborate (TNTU) linkage. The binding interaction between glycoproteins and self-assembled Con A is studied using horseradish peroxidase (HRP) as a model glycoprotein. Voltammetric, electrochemical impedance studies, and photometric activity measurements show the presence of both specific and nonspecific bindings of HRP to the Con A interface. The specific binding is attributed to the Con A - sugar interaction where Con A selectively recognizes the glycosylation sites of HRP. The catalytic current of the HRP-loaded electrode, because of catalytic oxidation of thionine in the presence of hydrogen peroxide (H2O2), is found to be proportional to the HRP concentrations in the incubation solution. A linear correlation coefficient of 0.993 was obtained over a wide HRP concentration range of 12.5 μg/mL to 1 mg/mL. The approach described in this study provides a simple yet selective means to immobilize glycoproteins on a solid support. The specific binding achieved is desirable in biosensor fabrication, glycoprotein separation, recognition, and purification as well as in drug-releasing systems. © 2007 American Chemical Society.
语种：
英文
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Recognition of Glycoprotein Peroxidase via Con A-Carrying Self-Assembly Layer on Gold
作者：
Kewei WANG;Dan DU;Songqin LIU
作者机构：
[Kewei WANG; Dan DU] Key Laboratory of Pesticide & Chemical Biology of Ministry of Education,Central China Normal University,Wuhan,430079,P.R.China;[Songqin LIU] School of Chemistry and Chemical Engineering,Southeast University,Nanjing,210096,P.R.China
会议名称：
The Eleventh International & The First Sino-Japan Bilateral Symposium on Electroanalytical Chemistry (11th ISEC & 1st SJBSEC)第十一届国际电分析化学学术会议暨第一届中日双边电分析化学学术会议
会议时间：
2007-8-16
会议地点：
长春
会议主办单位：
中国化学会
会议论文集名称：
The Eleventh International & The First Sino-Japan Bilateral Symposium on Electroanalytical Chemistry (11th ISEC & 1st SJBSEC)第十一届国际电分析化学学术会议暨第一届中日双边电分析化学学术会议论文集
语种：
英文
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Immobilization of acetylcholinesterase on gold nanoparticles embedded in sol–gel film for amperometric detection of organophosphorous insecticide
作者：
Du, Dan
* ;Chen, Shizhen;Cai, Jie;Zhang, Aidong
（ 期刊：
Biosensors and Bioelectronics ,2007年23(1):130-134 ISSN：0956-5663
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Wuhan Iron & Steel Co, Ctr Technol, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Amperometric sensor;Gold nanoparticles;Monocrotophos;Sol-gel
摘要：
A simple method to immobilize acetylcholinesterase (AChE) on silica sol-gel (SiSG) film assembling gold nanoparticles (AuNPs) was proposed, thus a sensitive, fast and stable amperometric sensor for quantitative determination of organophosphorous insecticide was developed. The large quantities of hydroxyl groups in the sol-gel composite provided a biocompatible microenvironment around enzyme molecule and stabilized its biological activity to a large extent. The immobilized AChE could catalyze the hydrolysis of acetylthiocholine chloride (ATCl) with a Kmapp value of 450 μM to form thiocholine, which was then oxidized to produce detectable single with a linear range of 10-1000 μM. AuNPs catalyzed the electro-oxidation of thiocholine, thus increasing detection sensitivity. Based on the inhibition of organophosphorous insecticide on the enzymatic activity of AChE, using monocrotophos as a model compound, the conditions for detection of the insecticide were optimized. The inhibition of monocrotophos was proportional to its concentration ranging from 0.001 to 1 μg/ml and 2 to 15 μg/ml, with the correlation coefficients of 0.9930 and 0.9985, respectively. The detection limit was 0.6 ng/ml at a 10% inhibition. The developed biosensor exhibited good reproducibility and acceptable stability, thus providing a new promising tool for analysis of enzyme inhibitors. © 2007 Elsevier B.V. All rights reserved.
语种：
英文
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Comparison of drug sensitivity using acetylcholinesterase biosensor based on nanoparticles-chitosan sol-gel composite
作者：
Du, Dan* ;Chen, Shizhen;Cai, Jie;Song, Dandan
期刊：
Journal of Electroanalytical Chemistry ,2007年611(1-2):60-66 ISSN：1572-6657
通讯作者：
Du, Dan
作者机构：
[Song, Dandan; Du, Dan; Chen, Shizhen] Cent China Normal Univ, Dept Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Cai, Jie] Wuhan Iron & Steel Co, Ctr Technol, Wuhan 430080, Peoples R China.;[Du, Dan] Cent China Normal Univ, Dept Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Luoyu Rd 2, Wuhan 430079, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Dept Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Luoyu Rd 2, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Biosensor;Drug sensitivity;Electrochemical test;Gold nanoparticles
摘要：
Based on the change in electrochemical behavior of enzymatic activity induced by medicines related to Alzheimer's disease (AD), a simple electrochemical method has been developed for investigation of medicine sensitivity using acetylcholinesterase (AChE) biosensor. The sol-gel-derived silicate network incorporating gold nanoparticles (AuNPs-SiSG) provided a biocompatible microenvironment around the enzyme molecule to stabilize its biological activity and prevent them from leaking out of the interface. AuNPs provided a conductive pathway for electron transfer and improved electrochemical reactions at a lower potential. Typical medicines for treatment of AD such as galantamine and neostigmine were selected for medicine sensitivity test. Due to the inhibitions of medicines, the electrochemical responses of substrate on AChE-sensor decreased greatly. The inhibition curves were similar to Michaelis-Menten and the Michaelis-Menten constants (K-m) were calculated to be 0.14 mu M and 0.19 mu M, respectively. The inhibition curves showed good correspondence with the results by UV spectrophotometry assay. Ninety-six percent reactivation of the inhibited AChE could be regenerated for using pralidoxime iodide within 8 min. The proposed electrochemical drug sensitivity test exhibited high sensitivity, low cost and simplified procedures, which provided a new promising tool for investigation of drug sensitivity. (C) 2007 Published by Elsevier B.V.
语种：
英文
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Amperometric Detection of Triazophos Pesticide Using Acetylcholinesterase Biosensor Based on Multiwall Carbon Nanotube-Chitosan Matrix
作者：
Dan DU;Xi HUANG;Aidong ZHANG
（ 作者机构：
[Aidong ZHANG; Dan DU; Xi HUANG] Key Laboratory of Pesticide & Chemical Biology of Ministry of Education,Central China Normal University,Wuhan 430079,P.R.China
会议名称：
The Eleventh International & The First Sino-Japan Bilateral Symposium on Electroanalytical Chemistry (11th ISEC & 1st SJBSEC)第十一届国际电分析化学学术会议暨第一届中日双边电分析化学学术会议
会议时间：
2007-8-16
会议地点：
长春
会议主办单位：
中国化学会
会议论文集名称：
The Eleventh International & The First Sino-Japan Bilateral Symposium on Electroanalytical Chemistry (11th ISEC & 1st SJBSEC)第十一届国际电分析化学学术会议暨第一届中日双边电分析化学学术会议论文集
语种：
英文
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Determination of carbaryl pesticide using amperometric acetylcholinesterase sensor formed by electrochemically deposited chitosan
作者：
Du, Dan
* ;Ding, Jiawang;Cai, Jie;Zhang, Aidong
（ 期刊：
Colloids and Surfaces B: Biointerfaces ,2007年58(2):145-150 ISSN：0927-7765
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Wuhan Iron & Steel Co, Ctr Technol, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Amperometric sensor;Carbaryl;Chitosan;Electrochemical deposition
摘要：
A sensitive, fast and cheap sensor for quantitative determination of carbaryl pesticide using amperometric acetylcholinesterase (AChE) sensor based on electrochemically deposited chitosan was reported. From a mildly acidic chitosan solution, a chitosan film is electrochemically deposited on Au electrode surface via a negative voltage bias, leading to a stable AChE sensor. The characteristics of the deposited layer were observed to be dependent upon the deposition time, pH, and the chitosan concentration. Fourier-transform infrared spectra proved that the immobilized enzyme could preserve their native structure due to the excellent biocompatibility and non-toxicity of chitosan. Under the optimal experimental conditions, the carbaryl inhibition on AChE-CHIT/Au was proportional to its concentration in two ranges, from 0.005 to 0.1 μg/ml and 0.5 to 5 μg/ml, with the correlation coefficients of 0.9966 and 0.9982, respectively. The detection limit was 0.003 μg/ml taken as the concentration equivalent to a 10% decrease in signal. The determination of carbaryl in garlic samples obtained from export of farm base showed acceptable accuracy. The developed sensor exhibited good fabrication reproducibility and acceptable stability, which provided a new promising tool for pesticide analysis. © 2007 Elsevier B.V. All rights reserved.
语种：
英文
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Amperometric detection of triazophos pesticide using acetylcholinesterase biosensor based on multiwall carbon nanotube–chitosan matrix
作者：
Du, Dan
* ;Huang, Xi;Cai, Jie;Zhang, Aidong
（ 期刊：
Sensors and Actuators B-Chemical ,2007年127(2):531-535 ISSN：0925-4005
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430070, Peoples R China.;Technol Ctr Wuhan Iron & Steel Co, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430070, Peoples R China.
关键词：
acetylcholinesterase;biosensor;chitosan;multiwall carbon nanotube;triazophos
摘要：
A simple method for immobilization of acetylcholinesterase (AChE) on multiwall carbon nanotubes (MWNTs)-chitosan (MC) composite was proposed and thus a sensitive, fast and stable amperometric, sensor for quantitative determination of organophosphorous insecticide was developed. Atomic force microscopy showed that this matrix possessed homogeneously netlike structure, which prevented enzyme from leaving out of the electrode. MWNTs promoted electron transfer reactions at a lower potential and catalyzed the electro-oxidation of thiocholine, thus increasing detection sensitivity. Based on the inhibition of organophosphorous insecticide to the enzymatic activity of AChE, using triazophos as a model compound, the conditions for detection of the insecticide were explored. Under optimal conditions, the inhibition of triazophos was proportional to its concentration in two ranges, from 0.03 to 7.8 mu M and 7.8 to 32 mu M with a detection limit of 0.01 mu M. A 95% reactivation of the inhibited AChE could be regenerated for using pralidoxime iodide within 8 min. The constructed biosensor processing prominent characteristics and performance such as good precision and reproducibility, acceptable stability and accuracy, fast response and low detection limit has potential application in the characterization of enzyme inhibitors and detection of toxic compounds against to enzyme. (c) 2007 Elsevier B.V. All rights reserved.
语种：
英文
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Electrochemical thiocholine inhibition sensor based on biocatalytic growth of Au nanoparticles using chitosan as template
作者：
Du, Dan
* ;Ding, Jiawang;Cai, Jie;Zhang, Aidong
（ 期刊：
Sensors and Actuators B-Chemical ,2007年127(2):317-322 ISSN：0925-4005
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Key Lab Pesticide & Chem Biol Minist Educ, Wuhan 430079, Peoples R China.;Technol Ctr Wuhan Iron & Steel Co, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol Minist Educ, Wuhan 430079, Peoples R China.
关键词：
acetylcholinesterase;biocatalytic growth;electrochemical sensor;electrodeposition;gold nanoparticles
摘要：
A simple strategy for the design of an electrochemical sensor based on enzyme-induced growth of gold nanoparticles (AuNPs) without adding gold nano-seeds was proposed with acetylcholinesterase (AChE) as a model. Owing to the excellent biocompatibility of chitosan film for the subsequent assembly of biological molecules, chitosan film was first electrochemically deposited on Au electrode surface from a mildly acidic chitosan solution via a negative voltage bias. Based on immobilization of AChE, a stable AChE sensor (AChE-CS/Au) was constructed. The immobilized AChE mediated hydrolysis of ATCl and yielded a reducing agent thiocholine. In the presence of AuCl4-, AuNPs were formed without addition of any metal nanoparticles. Atomic force microscopy (AFM) images proved that small spherical nanoparticles could grow on the enzyme-modified surface after incubation in HAuCl4 and ATCl solution. [Fe(CN)(6)](3-/4-) redox system was used as a probe to indicate the process of electron transfer across the interface and also analyze the enzyme inhibitor quantitatively. The inhibition of malathion on AChE was proportional to its concentration in the range of 0.1-500 ng ml(-1) (R= 0.9989), with detection limit of 0.03 ng ml(-1). (c) 2007 Elsevier B.V All rights reserved.
语种：
英文
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Rapid determination of triazophos using acetylcholinesterase biosensor based on sol-gel interface assembling muldwall carbon nanotubes
作者：
Du, Dan
* ;Cai, Jie;Song, Dandan;Zhang, Aidong
（ 期刊：
Journal of Applied Electrochemistry ,2007年37(8):893-898 ISSN：0021-891X
通讯作者：
Du, Dan
作者机构：
[Du, Dan] Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Technol Ctr Wuhan Iron & Steel Co, Wuhan 430080, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词：
Acetylcholinesterase;Amperometric determination;Biosensor;Multiwall carbon nanotube;Sol-gel;Triazophos
摘要：
A sensitive and stable amperometric sensor has been devised for rapid determination of triazophos based on efficient immobilization of acetylcholinesterase (AChE) on silica sol-gel (SiSG) film assembling multiwall carbon nanotubes (MWNTs). The sol-gel matrix provided a biocompatible microenvironment around the enzyme and efficiently prevented leakage of the enzyme from the film. In the presence of acetylthiocholine chloride (ATCl) as a substrate, MWNTs promoted electron transfer reactions at a lower potential and catalyzed electrochemical oxidation of enzymatically formed thiocholine, thus increasing detection sensitivity. Based on the inhibition of organophosphorous compound on the enzymatic activity of AChE, using triazophos as a model compound, the effects of pH, temperature, and MWNTs contents were explored. Under optimum conditions, the inhibition of triazophos was proportional to its concentration from 0.02 μM to 1 μM and from 5 μM to 30 μM, with a detection limit of 0.005 μM. The determination of triazophos in garlic samples showed acceptable accuracy. Fabrication reproducibility of the sensor was good and stability was acceptable. The sensor is a promising new tool for pesticide analysis. ©Springer Science+Business Media B.V. 2007.
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英文
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