期刊：
Biosensors and Bioelectronics,2017年89(Pt 1):43-55 ISSN：0956-5663
通讯作者：
Du, Dan;Lin, Yuehe
作者机构：
[Zhu, Chengzhou; Du, Dan; Lin, Yuehe; Du, D; Lin, YH] Minist Educ PR China, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Zhu, Chengzhou; Du, Dan; Lin, Yuehe; Du, D; Lin, YH] Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.;[Zhu, Chengzhou; Du, Dan; Lin, Yuehe] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.
通讯机构：
[Du, D; Lin, YH] M;[Du, D; Lin, YH] C;Minist Educ PR China, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.
作者机构：
[Cai, Xiaoli; Du, Dan; Lin, Yuehe; Luo, Yanan; Du, D; Lin, YH] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Du, Dan; Lin, Yuehe; Luo, Yanan; Du, D; Lin, YH] Washington State Univ, Sch Mech & Mat Engn, POB 642920, Pullman, WA 99164 USA.;[Zhang, Weiying] Jianghan Univ, Inst Interdisciplinary Res, Wuhan 430056, Peoples R China.;[Zhang, Weiying] Jianghan Univ, Key Lab Optoelect Chem Mat & Devices, Minist Educ, Wuhan 430056, Peoples R China.;[Du, Dan; Lin, Yuehe; Du, D; Lin, YH] Washington State Univ, Paul G Allen Sch Global Anim Hlth, POB 647090, Pullman, WA 99164 USA.
通讯机构：
[Du, D; Lin, YH] C;[Du, D; Lin, YH] W;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;Washington State Univ, Sch Mech & Mat Engn, POB 642920, Pullman, WA 99164 USA.;Washington State Univ, Paul G Allen Sch Global Anim Hlth, POB 647090, Pullman, WA 99164 USA.
作者机构：
[Liu, Xiangtao; Jiang, Tao] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Anim Virol,Minist Agr, Lanzhou 730046, Gansu, Peoples R China.;[Du, Dan; Jiang, Tao; Song, Yang; Lin, Yuehe] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.;[Lin, Yuehe] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA.;[Du, Dan] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticides & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Liu, Xiangtao] C;[Lin, Yuehe] W;Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Anim Virol,Minist Agr, Lanzhou 730046, Gansu, Peoples R China.;Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.;Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA.
关键词：
H2O2;immunochromatographic test strip;mesoporous Pt-Pd nanoparticles;peroxidase-like activity;TMB
作者机构：
[Cai, Xiaoli; Du, Dan; Luo, Yanan] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticides & Chem Biol, Wuhan 430079, Peoples R China.;[Li, He; Du, Dan; Lin, YH; Du, D; Lin, Yuehe] Washington State Univ, Sch Mech & Mat Engn, POB 642920, Pullman, WA 99164 USA.;[Du, Dan; Lin, YH; Du, D; Lin, Yuehe; Luo, Yanan] Washington State Univ, Paul G Allen Sch Global Anim Hlth, POB 647090, Pullman, WA 99164 USA.
通讯机构：
[Du, Dan] C;[Lin, YH; Du, D] W;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticides & Chem Biol, Wuhan 430079, Peoples R China.;Washington State Univ, Sch Mech & Mat Engn, POB 642920, Pullman, WA 99164 USA.;Washington State Univ, Paul G Allen Sch Global Anim Hlth, POB 647090, Pullman, WA 99164 USA.
期刊：
Biosensors and Bioelectronics,2016年76:195-212 ISSN：0956-5663
通讯作者：
Du, Dan
作者机构：
[Du, Dan; Song, Yang; Lin, Yuehe; Luo, Yanan] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Coll Chem, Wuhan 430079, Hubei Province, Peoples R China.;[Zhu, Chengzhou; Li, He; Du, Dan; Song, Yang; Lin, Yuehe; Luo, Yanan] Washington State Univ, Dept Mech & Mat Engn, Pullman, WA 99164 USA.;[Du, Dan; Lin, Yuehe] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA.;[Du, Dan] Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Hubei Province, Peoples R China.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Hubei Province, Peoples R China.
关键词：
DNA sensors;Electrochemical biosensor;Enzyme-based biosensors;Graphene;Immunosensors;Two-dimensional nanomaterials
作者机构：
[Yang, Enjian; Kang, Caiyan; Liang, Pei] Cent China Normal Univ, Coll Chem, Key Lab Pesticide Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Du, Dan; Lin, Yuehe; Ge, Xiaoxiao] Washington State Univ, Sch Mech & Mat Engn, POB 642920, Pullman, WA 99164 USA.
通讯机构：
[Liang, Pei] C;[Du, Dan] W;Cent China Normal Univ, Coll Chem, Key Lab Pesticide Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;Washington State Univ, Sch Mech & Mat Engn, POB 642920, Pullman, WA 99164 USA.
摘要：
We developed a new magnetic nanoparticle sandwich-like immunoassay using protein cage nanoparticles (PCN) for signal amplification together with graphite furnace atomic absorption spectrometry (GFAAS) for the quantification of an organophosphorylated acetylcholinesterase adduct (OP-AChE), the biomarker of exposure to organophosphate pesticides (OPs) and nerve agents. OP-AChE adducts were firstly captured by titanium dioxide coated magnetic nanoparticles (TiO2-MNPs) from the sample matrixes through metal chelation with phospho-moieties, and then selectively recognized by anti-AChE antibody labeled on PCN which was packed with lead phosphate in its cavity (PCN-anti-AChE). The sandwich-like immunoreaction was performed among TiO2-MNPs, OP-AChE and PCN-anti-AChE to form a TiO2-MNP/OP-AChE/PCN-anti-AChE immunocomplex. The complex could be easily isolated from the sample solution with the help of magnet, and the released lead ions from PCN were detected by GFAAS for the quantification of OP-AChE. Greatly enhanced sensitivity was achieved because PCN increased the amount of metal ions in the cavity of each apoferritin. The proposed immunoassay yielded a linear response over a broad range of OP-AChE concentrations from 0.01 nM to 2 nM, with a detection limit of 2 pM, which has enough sensitivity for monitoring of low-dose exposure to OPs. This new method showed an acceptable stability and reproducibility and was validated with OP-AChE spiked human plasma.
作者：
Ye, Ranfeng;Zhu, Chengzhou;Song, Yang;Lu, Qian;Ge, Xiaoxiao;...
期刊：
Small,2016年12(23):3094-3100 ISSN：1613-6810
通讯作者：
Du, Dan;Li, He;Lin, Yuehe
作者机构：
[Ye, Ranfeng; Du, Dan; Yang, Xu] Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.;[Ye, Ranfeng; Du, Dan; Yang, Xu] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Ye, Ranfeng; Zhu, Chengzhou; Lu, Qian; Li, He; Du, Dan; Song, Yang; Lin, Yuehe; Du, D; Lin, YH; Ge, Xiaoxiao] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99163 USA.;[Zhu, Mei-Jun] Washington State Univ, Sch Food Sci, Pullman, WA 99163 USA.;[Li, He] Univ Jinan, Sch Biol Sci & Technol, Jinan 250022, Peoples R China.
通讯机构：
[Du, Dan] C;[Du, D; Li, H; Lin, YH] W;[Li, He] U;Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
An innovatively bioinspired method has been developed for the green synthesis of multifunctional all-inone organic?inorganic hybrid nanoflowers integrating the functions of biorecognition and signal amplification. The synthesis of Con A-GOx-CaHPO<inf>4</inf>hybrid nanoflowers was carried out in a 1.5 mL eppendorf tube. In a typical synthesis, 0.2769 mg GOx and 0.0231 mg Con A were dissolved in 1 mL buffer solution. The products were purified by three repeated steps with centrifuging at 10 000 rpm for 5 min and then washing with DI water. For SEM observation, the suspension of the prepared nanoflowers was filtered and dried on a membrane and sputter coated with gold. For TEM observation, a drop of the suspension of the prepared nanofl owers was added to a carbon grid and dried at room temperature. Because the molecular weight of Cy5 and FITC is small and could be negligible compared with GOx and Con A, the encapsulation efficiency of GOx-Cy5 and Con A-FITC could be deemed as the encapsulation effi ciency of GOx and Con A, respectively.
摘要：
Currently, nano-biotechnology in the field of electrochemical biosensors has been a crucially novel strategy to construct simple and reliable monitoring systems for food safety. Due to the diversity of molecular species related to food safety, the characteristics of sensors must be designed according to concentration distribution level of target analyte, the specific reaction, the food source, and ease of operation. Therefore, the classification and characteristics of analytes for food safety (e.g., pesticides, veterinary drug residues, additives, inorganic and organic contaminants, pathogens and toxins) are clarified in this article. It focuses on an overview of electrochemical biosensors based on carbon nanotubes (CNTs), graphene (GR) and its derivatives, various metal nanoparticles, and polymers in food analyses. With the help of nanomaterials, the traditional advantages of electrochemical biosensors, such as rapidity, ease of fabrication and field applicability can be further improved. In addition, nanomaterials endow electrochemical biosensors with device miniaturization and high sensitivity and specificity, giving them great potential to assess the food safety on-site.
摘要：
Immunoassay is a common analytic method with great practicability. Recently, there is a growing interest in applying graphene to modern biosensor research due to its distinct physical and chemical properties, such as excellent conductivity, high surface area, ease of functionalization, and so on. In this review, we summarized the recent researches on the development of electrochemical immunoassays based on graphene. We focus on the different roles of graphene in constructing various electrochemical immunosensors and provide a perspective about this research field.
期刊：
Biosensors and Bioelectronics,2016年80:201-207 ISSN：0956-5663
通讯作者：
Du, Dan
作者机构：
[Zhang, Aidong; Du, Dan; Lin, Yuehe; Ge, Xiaoxiao] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Du, Dan; Lin, Yuehe; Ge, Xiaoxiao] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.;[Du, Dan; Lin, Yuehe] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
作者机构：
[Ye, Ranfeng; Li, He; Du, Dan; Yang, Xu] Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.;[Ye, Ranfeng; Li, He; Du, Dan; Yang, Xu] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Ye, Ranfeng; Zhu, Chengzhou; Li, He; Lu, Qian; Song, Junhua; Du, Dan; Song, Yang; Fu, Shaofang; Lin, Yuehe; Du, D; Lin, YH] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.;[Zhu, Mei-Jun] Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA.
通讯机构：
[Du, Dan] C;[Du, D; Lin, YH] W;Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.
作者机构：
[Yang, Haipeng; Zhu, Chengzhou; Du, Dan; Song, Yang; Lin, Yuehe; Shi, Qiurong] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.;[Du, Dan; Lin, Yuehe] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA.;[Du, Dan; Lin, Yuehe] Cent China Normal Univ, Coll Chem, Minist Educ PR China, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Lin, Yuehe] W;Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.
期刊：
Biosensors and Bioelectronics,2015年72:348-354 ISSN：0956-5663
通讯作者：
Du, Dan
作者机构：
[Zhao, Yuting; Du, Dan] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Du, Dan; Lin, Yuehe] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.;[Du, Dan; Lin, Yuehe] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA.
通讯机构：
[Du, Dan] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
关键词：
Glucometer;Glucose encapsulating liposome;Immunoassay;Phosphorylated protein
期刊：
CHEMICAL REVIEWS,2015年115(16):8896-8943 ISSN：0009-2665
通讯作者：
Lin, Yuehe
作者机构：
[Zhu, Chengzhou; Du, Dan; Lin, Yuehe] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.;[Du, Dan] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Eychmueller, Alexander] Tech Univ Dresden, Phys Chem, D-01062 Dresden, Germany.;[Lin, Yuehe] Pacific NW Natl Lab, Richland, WA 99352 USA.
通讯机构：
[Lin, Yuehe] W;Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.
摘要：
The use of nanoparticles (NPs) as building blocks and their assembly into functional architectures is of special relevance for realistic development and represents a rapidly growing branch of research. These fascinating porous noble metal nanostructures (PNMNs) provide a wealth of opportunities in multidisciplinary environments for promoting the rapid development of different research fields. These 3D porous architectures are able to provide higher specific surface areas and larger pore volumes, not only maximizing the availability of electron transfer within nanosized electrocatalyst surface area but also providing better mass transport of reactants to the electrocatalyst. Yamauchi and co-workers recently demonstrated a new approach, electrochemical micelle assembly, for constructing mesoporous Pt-based films through potentiostatic deposition. In this procedure, surfactant micelles can be effectively formed when the surfactant concentration added is above the CMC. Metal-aqua complexes, derived from the coordination between metal precursors and water, can usually interact with ethylene oxide groups of the surfactant micelles. Therefore, due to these structural directing agents, well-defined mesoporous films can be directly obtained on electrode surface via electrochemical deposition. Few reports have demonstrated the production of well-designed PNMNs with building blocks that are rich in high-index facets. With respect to electrochemical applications, PNMNs with hierarchically porous nanostructures hold great promise.
作者机构：
[Zhu, Junjie; Yang, Guohai] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China.;[Zhu, Chengzhou; Du, Dan; Lin, Yuehe; Yang, Guohai] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.;[Du, Dan; Lin, Yuehe] Cent China Normal Univ, Key Lab Pesticides & Chem Biol, Minist Educ PR China, Wuhan 430079, Peoples R China.
通讯机构：
[Zhu, Junjie] N;Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China.
摘要：
The development of nanotechnology provides promising opportunities for various important applications. The recent discovery of atomically-thick two-dimensional (2D) nanomaterials can offer manifold perspectives to construct versatile devices with high-performance to satisfy multiple requirements. Many studies directed at graphene have stimulated renewed interest on graphene-like 2D layered nanomaterials (GLNs). GLNs including boron nitride nanosheets, graphitic-carbon nitride nanosheets and transition metal dichalcogenides (e.g. MoS2 and WS2) have attracted significant interest in numerous research fields from physics and chemistry to biology and engineering, which has led to numerous interdisciplinary advances in nano science. Benefiting from the unique physical and chemical properties (e.g. strong mechanical strength, high surface area, unparalleled thermal conductivity, remarkable biocompatibility and ease of functionalization), these 2D layered nanomaterials have shown great potential in biochemistry and biomedicine. This review summarizes recent advances of GLNs in applications of biosensors and nanomedicine, including electrochemical biosensors, optical biosensors, bioimaging, drug delivery and cancer therapy. Current challenges and future perspectives in these rapidly developing areas are also outlined. It is expected that they will have great practical foundation in biomedical applications with future efforts.
