摘要：
目的：探索非致炎浓度的***对小鼠皮肤辣椒素受体（vanilloid receptor type 1，VR1）的激活作用。方法：选用KM小鼠随机分为3组：***（formalin，FM）组、辣椒素（capsaicin，CAP）组和Capsazepine（CPZ）组。采用注射后5min内小鼠舔舐注射脚掌的时间作为评价疼痛反应的指标，对各组小鼠的痛反应进行观测。结果：0．025％FM和200μmol／LCAP是理想的实验浓度，既能引起显著疼痛反应，又不引起炎性损伤的致痛浓度，适于研究受体拮抗剂的抗疼痛作用；1μmol／LCPZ分别与200μmol／LCAP和0．025％FM共注射能有效的拮抗CAP和FM引起的疼痛反应。结论：非致炎浓度FM所致的疼痛反应在外周可能由VR1介导。
摘要：
为研究类香草素受体（vanilloid receptor type 1，VR1）作为刺激性室内空气污染物的触发性生物靶分子的生理作用，在VR1-cDNA质粒基础上。应用分子克隆和细胞转染技术构建了体外培养的VR1^＋-HEK293细胞模型，应用此细胞模型进行辣椒素和甲醛致VR1^＋-HEK293细胞死亡实验．结果表明，VR1^＋-HEK293细胞模型构建成功．VR1^＋细胞死亡实验是一种简便有效的鉴定VR1体外表达的实验方法；甲醛能够激活VR1，且激活反应能被VR1特异性拮抗剂capsazepine所阻断，从而推断甲醛对气道的刺激作用也是通过VR1所介导的．用体外细胞学实验证实了VR1是甲醛作用的生物靶分子，以实验证实了Nielsen假说的正确性．
摘要：
目的研究小鼠吸入气态甲醛后,甲醛对小鼠的脑、肾组织蛋白造成氧化损伤和脂质的过氧化及其发生机制。方法用不同剂量的气态甲醛对小鼠进行连续动态染毒处理72 h,用2,4-二硝基苯肼比色法测定小鼠脑、肾组织蛋白质的羰基含量,以判断蛋白质的氧化损伤程度;用M DA（丙二醛）试剂盒测定组织中M DA含量,来判断脂质的过氧化程度。结果只有吸入3.0 m g/m3的气态甲醛时,才会对脑、肾组织中的蛋白质造成明显的氧化损伤;而在0.5 m g/m3时,脑组织中脂质的过氧化比肾组织中显著。结论0.5 m g/m3的气态甲醛不造成蛋白质氧化损伤,而对脑组织中脂质的过氧化作用却很显著,可见0.5 m g/m3甲醛对生物体存在着直接的神经毒性;3.0 m g/m3的气态甲醛对脑、肾组织中的蛋白质和脂质会造成明显的氧化损伤,且肾组织比脑组织敏感。
摘要：
The prevalence of asthma keeps on increasing worldwide, especially in western societies over last 40 years. The mechanism of asthma is unclear. Recently, concern about indoor air pollution as a risk factor for asthma has been arisen. In present study, 25 Kun Ming male mice were placed in an air chamber containing respective formaldehyde ( FA) concentration of 0, 0.5, 1.0, 3.0 mg/m(3), and 3.0 mg/m3 with Capsazepine ( CPZ, a specific antagonist of vanilloid receptor)-pretreatment in five testing groups ( n= 5 per group) for inhale experiments. The inhaled groups were exposed to gaseous FA for 6 hours each day in 10 successive days. After exposure, the concentrations of IL4 in blood serum and broncho alveolar lavage fluid (BALF) were measured. Experimental results showed that the IL4 level in serum was too low to be detected; and the concentrations of IL4 in BALF increased in a dose-dependent manner. However, for the CPZ-pretreated group the IL4 level in BALF decreased significantly ( compared with 3.0 mg/m(3) FA inhaled group, p < 0.01). This paper describes experimental animal methods to probe IL4 level, an important indicator for IgE response. The studies in this paper indicated that gaseous FA might induce acquired atopy by type II VR1 signaling system. These findings suggested that indoor air pollutants such as FA might be key risk factors for the rise in asthma cases, and type II VR1 signaling system might be one of the mechanisms for the rise.
期刊：
INDOOR AIR 2005: PROCEEDINGS OF THE 10TH INTERNATIONAL CONFERENCE ON INDOOR AIR QUALITY AND CLIMATE, VOLS 1-5,2005年15:3920-3923 ISSN：0908-5920
通讯作者：
Yang, X
作者机构：
[Duan, LJ; Lu, ZS; Yan, Y; Cao, Y; Yang, X; Wang, GX] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Yang, X] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
第十届室内空气品质和质量国际学术会议(Proceedings of the 10th International Conference on Indoor Air Quality and Climate)
会议时间：
2005-09-04
会议地点：
北京
会议主办单位：
Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
会议论文集名称：
第十届室内空气品质和质量国际学术会议
关键词：
formaldehyde;hormesis;biphasic;dual effects
摘要：
Hormesis is a dose-response phenomenon, which is characterized by a counterintuitive switchover from low-dose stimulation to high-dose inhibition that is not infrequently encountered in the course of a toxicity assay. In this paper we provided various data, mainly our findings, to show that hormesis is a common phenomenon in the study of formaldehyde toxicity and these findings have great implications affecting study design, animal model, and endpoint selection as well as clinical applications. In the central nervous system, it has been noticed that formaldehyde can do harm to learning and memory abilities. Our findings in mice exposure to I and 3 mg/m(3) formaldehyde testified this. It is interested to notice, however, mice exposure to 0.5 mg/m(3) formaldehyde have better memory ability than control group. It has also been noticed that formaldehyde can induce the damage of supermolecules such as DNA and proteins, because formaldehyde is highly chemically active.
摘要：
The purpose of the present study was to determine gene expression pattern of chemosensitive receptor VR1 in the brain stem of the neonatal rat after low-level gaseous formaldehyde exposure. Male Sprague-Dawley newborn rats were exposed to clean air or 1.8 mg/m(3) formaldehyde for consecutive 72 h and the brain stems were dissected for RNA extraction. The mRNA levels of VR1 and cytokine NGF gene were determined with semiquantitative RT-PCR. We observed that the mRNA expression of VR1 and NGF was significantly increased in the brain of the tested neonatal rats. These findings suggest that successive formaldehyde exposure may increase cytokine NGF expression and then NGF up-regulates VR1 gene expression synchronously. This may have implications for the nervous central neuro-immunological mechanisms mediating multiple chemical sensitivity (MCS), sick building syndromes (SBS), bronchial hyperresponsiveness (BHR) and asthma in humans.
