摘要：
目的检测经不同浓度气态甲醛暴露后所致小鼠脑细胞的遗传损伤。方法用不同剂量气态甲醛（0.5,1.0和3.0 m g/m3）对小鼠进行连续动态染毒处理72 h,利用单细胞凝胶电泳技术（又称彗星实验）检测脑细胞的DNA损伤。结果吸入浓度为0.5和1.0 m g/m3的甲醛后,可造成脑细胞DNA的严重断裂,而3.0 m g/m3的甲醛则引起显著的交联作用。结论气态甲醛可造成脑细胞DNA的断裂和交联。
摘要：
Formaldehyde (FA) is a genotoxic and mutagenic substance. In 2004, IARC (International Agency for Research on Cancer) concluded that FA is carcinogenic in humans after reevaluating the available evidence on the carcinogenicity of FA. Although many studies have shown that FA had extensive genotoxicity including DNA-protein crosslinks (DPC) and DNA single strand breaks (DSSB), most of these studies only discussed the effects of FA at high levels. In this study, KCl-SDS assay and single cell gel electrophoresis (SCGE) were used to investigate the formation and repair process of FA-induced DPC and DSSB in human peripheral blood lymphocytes and Hela cell lines. KCl-SDS assay was applied to detect DPC induced by liquid FA in human peripheral blood lymphocytes in vitro. The results showed that FA could induce DPC at high levels (≥50 micro M). By combining the results of KCl-SDS assay and SCGE, it could be determined that FA would induce DNA-DNA crosslinks (DDC) when FA concentration was more than 25 microM. The repair process of FA-induced DPC was studied with KCl-SDS assay in Hela cell lines and the results demonstrated that FA-induced DPC could be significantly repaired after 18 hours. The SCGE was also used to determine FA-induced DSSB and its repair process in Hela cell lines. The results demonstrated that DNA breakages, which is capable of being induced by FA at a low level (<30 microM), enabled to be repaired completely in 90 minutes.
作者：
Li Ping;Liu De-li*;Nahimana Liberat;Chen Shu-li;Yang Xi;...
期刊：
环境科学学报(英文版),2006年18(3):525-529 ISSN：1001-0742
通讯作者：
Liu De-li
作者机构：
[Li Ping; Chen Shu-li; Liu De-li; Yang Xi; Zhao Li; Liu, DL; Nahimana Liberat] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Liu De-li] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
关键词：
high nitrogen removal;aerobic denitrification;wastewater treatment;identification
摘要：
Three new bacteria HS-03, HS-043 and HS-047 isolated from different ecosystems were found capable of aerobic denitrification. The potential application of these strains in wastewater treatment under aerobic conditions was investigated. These three bacteria all presented high nitrogen removal from wastewater that more than 98% of 10 mmol/L nitrate could be removed in 12-24 h by adding cheap external carbon source and low concentration of iron as well as molybdate. The mechanism at molecular level was analyzed. The success of this aerobic denitrification applied to wastewater treatment may serve as an alternative to enhance the practical nitrogen removal from wastewater. Main biochemical and physiological features of these strains were characterized. The 16S rDNA sequences were compared with the published data in GenBank by using BLAST. The results of phenotype and genotype proved that strain HS-03 and HS-047 belonged to Pseudomonas stutzeri and Pseudomonas pseudoalcaligenes respectively. Strain HS-043 was identified as Delftia acidovorans of which denitrifying activity has not previously been explored.
关键词：
small interfering RNA;adhesion molecule CD146;vascular endothelial cell
摘要：
Our previous study has demonstrated that CD146 molecule is a biomarker on vascular endothelium, which is involved in angiogenesis and tumor growth. However the mechanism behind is not clear. Here we have for the first time developed a novel CD146 blockade system using CD146 siRNA to study its function on endothelial cells. Our data showed that CD146 siRNA specifically blocked the expression of CD146 on both mRNA and protein levels, leading to the significant suppression of HUVEC proliferation, adhesion and migration. These results demonstrate that CD146 plays a key role in vascular endothelial cell activity and angiogenesis, and CD146 siRNA can be used as a new inhibitor for anti-angiogenesis therapy.
摘要：
目的从分子水平研究中等浓度气态甲醛环境暴露对呼吸系统中类香草素受体（van illo id recep-tor sub type 1,VR 1）基因表达的影响。方法用WH-2型环境气候舱释放的甲醛和净空气,分别通入2只各放有5只幼年大鼠的染毒缸中连续灌流72 h后,迅速取肺,用RT-PCR方法检测大鼠在中等浓度甲醛环境暴露下肺VR 1和管家基因G 3PD表达水平。结果中等浓度甲醛3.0 m g/m3灌流比清洁空气灌流导致幼年大鼠肺VR 1-mRNA表达增加。结论中等浓度甲醛灌流导致幼年大鼠肺部VR 1-mRNA表达量增加。
