摘要：
In order to improve the efficiency of comet assay, which can be used in epidemiological research to detect genotoxicity of air pollutants, we undertook this study. Formaldehyde at 50 mu M was employed as contaminating agent to poison the human buccal cell before comet assay. During the process of comet assay, each pair of contaminated samples was treated with proteinase K of three different concentrations at 0.1mg/ml, 1mg/ml and 10mg/ml, respectively while other steps are kept the same. The result turned out to be that the 1mg/ml proteinase K could lead to an ideal incubating effect. It was also proved that a 45-minute lysis had almost the same effect as the 120-min one. A further try was the reduction of the enzyme-incubation period to 45 min; the result showed that with the treatment of 1mg/ml proteinase K, a 45-minute lysis and a 45-minute enzyme-incubation as well can achieve a relatively ideal result.
摘要：
A simple and sensitive electrochemical immunosensor with impedance labelless detection and novel data processing method was investigated. One-step copolymerization was used to electrochemically deposit an antibody impregnated polypyrrole film on a glassy carbon electrode surface for the immunosensor. Impedance measurements provided a labelless or reporterless method to detect antibody (Ab)-antigen (Ag) interactions. Dimensionless analysis was employed to successfully process the measured impedance data. Since the method derived unit impedance change to eliminate or reduce the variation of the bulk electronic properties of Ab/polypyrrole films, the signal to noise ratio (S/N) was significantly improved for high sensitivity and specificity. Nonspecific binding effect was studied by array electrode chips and was found out that the polypyrrole electrode without antibody attachment had much stronger nonspecific binding effect than the Ab/polypyrrole electrode; incubation followed by thoroughly washing significantly reduced the nonspecific interference. 10 pg/ml detection limit and superior specificity were achieved by the method, demonstrating a highly sensitive labelless immunosensor in comparison with the detection limit of ng -microgram/ml for the reported polypyrrole based immunosensors. The electrochemical immunosensors presented in this paper, due to its simplicity, low cost, high sensitivity and superior specificity, could be an invaluable tool for clinical diagnostics and could have potential applications in drug discovery, environmental and food analysis.
摘要：
Genomic DNA methylation is one of the most important epigenetic mechanisms. Existing data of indoor air formaldehyde effect on DNA methylation patterns is limited. S-adenosylmethionine, the sole methyl donor in this reaction, is generally connected to the tetrahydrofolate and the methionine-homocysteine cycle and one carbon metabolism. In mammalian systems, as a normal intermediary metabolite, endogenous formaldehyde remains in equilibrium in blood and tissues. There are two ways that endogenous FA can be involved in one carbon pool. One is the production of 5, 10-methlene-THF formed by FA and THF. The second way is through the formation of 10-formyl-THF. Experiments indicate that the oxidation of 10-formyl-THF to 10-formyl-dihydrofol ate takes place and 10-formyl-dihydrofolate is subsequently converted to dihydrofolate. Therefore, in light of those circles, we hypothesize that endogenous FA can act as one carbon group to participate in the process of DNA methylation or may cause demethylation through free radical injures.
期刊：
INDOOR AIR 2005: PROCEEDINGS OF THE 10TH INTERNATIONAL CONFERENCE ON INDOOR AIR QUALITY AND CLIMATE, VOLS 1-5,2005年15:3813-3816 ISSN：0908-5920
通讯作者：
Yang, X
作者机构：
[Qiao, Y; Lu, ZS; Yan, Y; Li, CM; Gan, YK; Hou, LL; Yang, X] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Yang, X] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
10th International Conference on Indoor Air Quality and Climate (Indoor Air 2005)
会议时间：
SEP 04-09, 2005
会议地点：
Beijing, PEOPLES R CHINA
会议主办单位：
Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
关键词：
formaldehyde;oxidative damage;SOD;GSK;MDA
摘要：
Few researches concern about oxidative damage of inhaled formaldehyde in medium or low concentrations. Therefore, we treated three groups of mice with 1.0 mg/m(3) 3.0 mg/m(3) formaldehyde and the control group inhaled fresh air. All of the mice were treated 6 hours per day for 10 days. After exposure, the activities of total superoxide dismutase (SOD), content of glutathione (GSH) and malondialdehyde (MDA) in five organs-brain, heart, lung, liver and kidney were measured. The results showed that inhaled formaldehyde caused the obvious decrease of SOD activities in five organs (P<0.01) in 3.0 mg/m(3) group, and the level of GSH in all treated groups were decreased signficantly in various organs (P<0.05) compared with fresh air group. The level of MDA in all organs of 3.0 mg/m(3) were increased significantly (p<0.05) compared with fresh air group. In conclusion, medium concentration formaldehyde gas induces significantly oxidative damage of various organs.
期刊：
INDOOR AIR 2005: PROCEEDINGS OF THE 10TH INTERNATIONAL CONFERENCE ON INDOOR AIR QUALITY AND CLIMATE, VOLS 1-5,2005年15:3556-3559 ISSN：0908-5920
通讯作者：
Li, R
作者机构：
[Chen, WG; Wei, F; Peng, JS; Wu, L; Hu, JC; Zhou, X; Q Z; Yao, DS; Liu, MQ; Yang, X; Li, R] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Li, R] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
10th International Conference on Indoor Air Quality and Climate (Indoor Air 2005)
会议时间：
SEP 04-09, 2005
会议地点：
Beijing, PEOPLES R CHINA
会议主办单位：
Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
关键词：
formaldehyde;genotoxicity;comet assay;buccal cell;epidemiological study
摘要：
In this study, 60 families in the same community were investigated. Amnong these families, 30 were decorated within one year while the corresponding ones decorated at least 4 years ago. Buccal cells donated by people available in these families were employed to carry out single cell gel electrophoresis (SCGE, Comet assay, for genotoxicity testing). Analysis of the comet images showed that the presenting rate of "comet" as well as the tail moments of the buccal cells' DNA from people living in apartments decorated within a year were obviously higher (P < 0.01) than in those from people who lived in the apartments decorated several years ago, which lends strong support to our conclusion that people living in newly-decorated apartments are much more liable to be harmed by the formaldehyde released from their indoor decoration. In addition, high-quality decoration material tends to be less harmful to people.
摘要：
To explore protein oxidative damage induced by gaseous formaldehyde and its mechanism we carried out this study. Mice were treated with gaseous formaldehyde at different levels (0 mg.m(-3) 0.68 mg.m(-3) and 3.0 mg.m(-3)) for 72 hr and the protein carbonyl content was mensurated using spectrophotometric DNPH assay to reflect the degree of protein oxidative damage. The results show that the protein carbonyl content of brain, heart and liver tissue in mice decrease in the group of 0.68 mg.m(-3) (p < 0.01, p < 0.01, p < 0.05), while increase significantly in heart and liver tissue in the group of 3.0 mg.m(-3) (P < 0.01, P < 0.01) and protein carbonyl content in brain does not have notable difference compared with the control (p > 0.05). These results indicate that the protein oxidative damage induced by gaseous formaldehyde depends on the concentration of gaseous formaldehyde. Medium concentration formaldehyde may not induce protein oxidative damage, while high concentration formaldehyde can cause protein oxidative damage in heart and liver tissue markedly and has little effect on protein of brain.
期刊：
INDOOR AIR 2005: PROCEEDINGS OF THE 10TH INTERNATIONAL CONFERENCE ON INDOOR AIR QUALITY AND CLIMATE, VOLS 1-5,2005年:3831-3835
通讯作者：
Yang, X
作者机构：
[Qiao, Y; Liu, Y; Duan, L; Yan, Y; Lu, Z; Cao, Y; Yang, X] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Yang, X] C;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
第十届室内空气品质和质量国际学术会议(Proceedings of the 10th International Conference on Indoor Air Quality and Climate)
会议时间：
2005-09-04
会议地点：
北京
会议论文集名称：
第十届室内空气品质和质量国际学术会议
关键词：
Formaldehyde;Toxicological study;Physiological study
摘要：
This paper is a review on recent developments of the toxicological and physiological studies on formaldehyde. Hotspots involved in this topic include: (1) Formaldehyde-induced gene expression; (2) Formaldehyde-induced asthma; (3) Airway neurogenic inflammation (4) formaldehyde working as signaling molecule in body; (5) Epigenesis: methylation of DNA and proteins; (6) DNA-protein crosslink; (7) Lysine-DPC repair enzyme; (8) Formaldehyde-induced Apoptosis.
